scholarly journals Functional Analysis of Intergenic Regions for Gene Discovery

10.5772/21402 ◽  
2011 ◽  
Author(s):  
Li M.
Keyword(s):  
Functional Analysis ◽  
Gene Discovery ◽  
Intergenic Regions

scholarly journals Chromosome substitution strains: gene discovery, functional analysis, and systems studies

2012 ◽  
Vol 23 (9-10) ◽  
pp. 693-705 ◽  
Author(s):  
Joseph H. Nadeau ◽  
Jiri Forejt ◽  
Toyoyuki Takada ◽  
Toshihiko Shiroishi
Keyword(s):  
Functional Analysis ◽  
Gene Discovery ◽  
Chromosome Substitution ◽  
Chromosome Substitution Strains ◽  
Systems Studies

Balanced-Size and Long-Size Cloning of Full-Length, Cap-Trapped cDNAs into Vectors of the Novel λ-FLC Family Allows Enhanced Gene Discovery Rate and Functional Analysis

Genomics ◽  
2001 ◽  
Vol 77 (1-2) ◽  
pp. 79-90 ◽  
Author(s):  
Piero Carninci ◽  
Yuko Shibata ◽  
Norihito Hayatsu ◽  
Masayoshi Itoh ◽  
Toshiyuki Shiraki ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Gene Discovery ◽  
Full Length ◽  
The Novel

REP-elements of the Escherichia coli Genome and Transcription Signals: Positional and Functional Analysis

2015 ◽  
Vol 10 (1) ◽  
pp. 245-259
Author(s):  
Н.Ю. Маркелова ◽  
N.Yu. Markelova
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Rna Synthesis ◽  
Transcription Activation ◽  
Specific Distribution ◽  
Preferential Localization ◽  
Intergenic Regions ◽  
Functional Autonomy ◽  
The Many ◽  
Transcription Signals

In the intergenic regions of the Escherichia coli genome there are 356 REP- elements, containing 1-12 repeated sequences with degenerated consensus. Their biological role is poorly understood, but multiplicity in the genome, preferential localization between convergent genes and ability to form hairpin structures have led to the assumption that REP-elements participate in the transcription termination and processes affecting stability of the corresponding RNAs. Though the direct experiments did not confirm the ability of the model REP-sequence to stop RNA synthesis leaving some ambiguity regarding their primary function. In this study, positional and functional analysis was undertaken for the entire set of annotated REP-sequences and the reduced efficiency of RNA synthesis behind the many REP-modules was observed. However, some REP-modules had no effect on the transcription processivity, assuming the inclusion of REP-sequences into RNA and the possibility of their regulatory action. We also observed REP-associated transcription activation and found overlapping promoters. The most unexpected was specific distribution of REP-sequences nearby promoter islands, which indicates their insulator-like action maintaining functional autonomy of the “islands” and assumes functional significance of “island”-born RNAs.

Sequence and Functional Analysis of the Intergenic Regions Separating Babesial Rhoptry-Associated Protein-1 (rap-1)Genes

1998 ◽  
Vol 90 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Carlos E. Suarez ◽  
Guy H. Palmer ◽  
Isidro Hötzel ◽  
Stephen A. Hines ◽  
Terry F. McElwain
Keyword(s):  
Functional Analysis ◽  
Intergenic Regions
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