Balanced-Size and Long-Size Cloning of Full-Length, Cap-Trapped cDNAs into Vectors of the Novel λ-FLC Family Allows Enhanced Gene Discovery Rate and Functional Analysis

Genomics ◽  
2001 ◽  
Vol 77 (1-2) ◽  
pp. 79-90 ◽  
Author(s):  
Piero Carninci ◽  
Yuko Shibata ◽  
Norihito Hayatsu ◽  
Masayoshi Itoh ◽  
Toshiyuki Shiraki ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Gene Discovery ◽  
Full Length ◽  
The Novel

scholarly journals Full-length transcriptome analysis reveals the mechanism of acupuncture at PC6 improves cardiac function in myocardial ischemia model

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Jing Yuan ◽  
Jun-Meng Wang ◽  
Zhi-Wei Li ◽  
Cheng-Shun Zhang ◽  
Bin Cheng ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Cardiac Function ◽  
Acupuncture Treatment ◽  
Pathological Process ◽  
Full Length ◽  
The Novel ◽  
New Genes ◽  
Myocardial Infraction ◽  
Differential Expression Genes ◽  
Multi Level

Abstract Background The pathological process of myocardial ischemia (MI) is very complicated. Acupuncture at PC6 has been proved to be effective against MI injury, but the mechanism remains unclear. This study investigated the mechanism that underlies the effect of acupuncture on MI through full-length transcriptome. Methods Adult male C57/BL6 mice were randomly divided into control, MI, and PC6 groups. Mice in MI and PC6 group generated MI model by ligating the left anterior descending (LAD) coronary artery. The samples were collected 5 days after acupuncture treatment. Results The results showed that treatment by acupuncture improved cardiac function, decreased myocardial infraction area, and reduced the levels of cTnT and cTnI. Based on full-length transcriptome sequencing, 5083 differential expression genes (DEGs) and 324 DEGs were identified in the MI group and PC6 group, respectively. These genes regulated by acupuncture were mainly enriched in the inflammatory response pathway. Alternative splicing (AS) is a post-transcriptional action that contributes to the diversity of protein. In all samples, 8237 AS events associated with 1994 genes were found. Some differential AS-involved genes were enriched in the pathway related to heart disease. We also identified 602 new genes, 4 of which may the novel targets of acupuncture in MI. Conclusions Our findings suggest that the effect of acupuncture on MI may be based on the multi-level regulation of the transcriptome.

scholarly journals Full-length and C-terminal neurogranin in Alzheimer’s disease cerebrospinal fluid analyzed by novel ultrasensitive immunoassays

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Annika Öhrfelt ◽  
Julien Dumurgier ◽  
Henrik Zetterberg ◽  
Agathe Vrillon ◽  
Nicholas J. Ashton ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Single Molecule ◽  
Full Length ◽  
The Novel ◽  
Drug Candidates ◽  
Postsynaptic Protein ◽  
Coefficients Of Variation ◽  
Assay Precision ◽  
Novel Drug

Abstract Background Neurogranin (Ng) is a neuron-specific and postsynaptic protein that is abundantly expressed in the brain, particularly in the dendritic spine of the hippocampus and cerebral cortex. The enzymatic cleavage of Ng produces fragments that are released into cerebrospinal (CSF), which have been shown to be elevated in Alzheimer’s disease (AD) patients and predict cognitive decline. Thus, quantification of distinctive cleavage products of Ng could elucidate different features of the disease. Methods In this study, we developed novel ultrasensitive single molecule array (Simoa) assays for measurement of full-length neurogranin (FL-Ng) and C-terminal neurogranin (CT-Ng) fragments in CSF. The Ng Simoa assays were evaluated in CSF samples from AD patients (N = 23), mild cognitive impairment due to AD (MCI-AD) (N = 18), and from neurological controls (N = 26). Results The intra-assay repeatability and inter-assay precision of the novel methods had coefficients of variation below 7% and 14%, respectively. CSF FL-Ng and CSF CT-Ng median concentrations were increased in AD patients (6.02 ng/L, P < 0.00001 and 452 ng/L, P = 0.00001, respectively) and in patients with MCI-AD (5.69 ng/L, P < 0.00001 and 566 ng/L, P < 0.00001) compared to neurological controls (0.644 ng/L and 145 ng/L). The median CSF ratio of CT-Ng/FL-Ng were decreased in AD patients (ratio = 101, P = 0.008) and in patients with MCI-AD (ratio = 115, P = 0.016) compared to neurological controls (ratio = 180). CSF of FL-Ng, CT-Ng, and ratio of CT-Ng/FL-Ng could each significantly differentiate AD patients from controls (FL-Ng, AUC = 0.907; CT-Ng, AUC = 0.913; CT-Ng/FL-Ng, AUC = 0.775) and patients with MCI-AD from controls (FL-Ng, AUC = 0.937; CT-Ng, AUC = 0.963; CT-Ng/FL-Ng, AUC = 0.785). Conclusions Assessments of the FL-Ng and CT-Ng levels in CSF with the novel sensitive immunoassays provide a high separation of AD from controls, even in early phase of the disease. The novel Ng assays are robust and highly sensitive and may be valuable tools to study synaptic alteration in AD, as well as to monitor the effect on synaptic integrity of novel drug candidates in clinical trials.

Comic Collisions: Convention, Rage, and Order

1987 ◽  
Vol 3 (12) ◽  
pp. 303-316 ◽  
Author(s):  
Susan Carlson
Keyword(s):  
Twentieth Century ◽  
Henry James ◽  
Associate Professor ◽  
Full Length ◽  
The Novel ◽  
State University ◽  
Modern Drama ◽  
Happy Ending ◽  
Critical Aspects

How can the socially critical aspects of comedy be reconciled with a ‘happy ending’ which seems to affirm the existing order of things? This perennial problem has become acute in a period when both playwrights and comic performers are increasingly conscious of the dangers inherent in the stereotyping – racial, sexual, and hierarchical – on which so much comedy depends. In this article, Susan Carlson looks at some recent ‘meta-comedies’ which have used the form, as it were, to expose itself – notably, Trevor Griffiths's Comedians, Peter Barnes's Laughter, Susan Hayes's Not Waving, and Caryl Churchill's Cloud Nine – and analyzes their responses to comedy, which range from the despairing to the affirmative. She concludes that only Churchill has found a positive way of ‘connecting the painful recognitions of twentieth-century dissociations to comic hope’. Susan Carlson is Associate Professor of English at lowa State University. In addition to numerous articles on modern drama and the novel, she has published a full-length study of the plays of Henry James, and is currently working on a book about women in comedy.

scholarly journals Chromosome substitution strains: gene discovery, functional analysis, and systems studies

2012 ◽  
Vol 23 (9-10) ◽  
pp. 693-705 ◽  
Author(s):  
Joseph H. Nadeau ◽  
Jiri Forejt ◽  
Toyoyuki Takada ◽  
Toshihiko Shiroishi
Keyword(s):  
Functional Analysis ◽  
Gene Discovery ◽  
Chromosome Substitution ◽  
Chromosome Substitution Strains ◽  
Systems Studies

THE CHEMOKINE C10: IMMUNOLOGICAL AND FUNCTIONAL ANALYSIS OF THE SEQUENCE ENCODED BY THE NOVEL SECOND EXON

Cytokine ◽  
1996 ◽  
Vol 8 (6) ◽  
pp. 439-447 ◽  
Author(s):  
Mark S. Berger ◽  
Dennis D. Taub ◽  
Amos Orlofsky ◽  
Thomas R. Kleyman ◽  
Brigitte Coupaye-Gerard ◽  
...  
Keyword(s):  
Functional Analysis ◽  
The Novel

Isolation, characterization and functional analysis of full length p53 cDNA from Bubalus bubalis

Gene ◽  
2015 ◽  
Vol 568 (2) ◽  
pp. 146-154 ◽  
Author(s):  
Minu Singh ◽  
Suruchi Aggarwal ◽  
Ashok K. Mohanty ◽  
Tapas Mukhopadhyay
Keyword(s):  
Functional Analysis ◽  
Bubalus Bubalis ◽  
Full Length

scholarly journals Gardos channelopathy: functional analysis of a novel KCNN4 variant

2020 ◽  
Vol 4 (24) ◽  
pp. 6336-6341
Author(s):  
Elisa Fermo ◽  
David Monedero-Alonso ◽  
Polina Petkova-Kirova ◽  
Asya Makhro ◽  
Laurent Pérès ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Diagnostic Markers ◽  
The Novel ◽  
Clinical Heterogeneity ◽  
Gain Of Function ◽  
Key Points

Key Points We show that the novel KCNN4 variant p.S314P is a gain-of-function mutation but is less severe than the previously reported p.R352H variant. The clinical heterogeneity, blurred symptoms, and absence of specific diagnostic markers make the diagnosis of Gardos channelopathy challenging.

scholarly journals Positive selection of ORF3a and ORF8 genes drives the evolution of SARS-CoV-2 during the 2020 COVID-19 pandemic

2020 ◽  
Author(s):  
Lauro Velazquez-Salinas ◽  
Selene Zarate ◽  
Samantha Eberl ◽  
Douglas P. Gladue ◽  
Isabel Novella ◽  
...  
Keyword(s):  
Public Health ◽  
Positive Selection ◽  
Evolutionary Dynamics ◽  
Full Length ◽  
Accessory Proteins ◽  
The Novel ◽  
Phylogenetic Groups ◽  
Epistatic Interactions ◽  
Genetic Mechanisms ◽  
Selection Of

AbstractIn this study, we analyzed full-length SARS-CoV-2 genomes from multiple countries to determine early trends in the evolutionary dynamics of the novel COVID-19 pandemic. Results indicated SARS-CoV-2 evolved early into at least three phylogenetic groups, characterized by positive selection at specific residues of the accessory proteins OFR3a and ORF8a. We also report evidence of epistatic interactions among sites in the genome that may be important in the generation of variants adapted to humans. These observations might impact not only public health, but also suggest more studies are needed to understand the genetic mechanisms that may affect the development of therapeutic and preventive tools, like antivirals and vaccines.

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