scholarly journals Hide and Go Seek: Activation of the Secretory-Specific Poly (A) Site of Igh by Transcription Elongation Factors

10.5772/21186 ◽  
2011 ◽  
Author(s):  
Christine Milcarek
Keyword(s):  
Transcription Elongation ◽  
Elongation Factors ◽  
A Site

CHAPTER 4. RNA Polymerase-associated Transcription Elongation Factors

2021 ◽  
pp. 72-99
Author(s):  
Shun-ichi Sekine ◽  
Tamami Uejima ◽  
Haruhiko Ehara
Keyword(s):  
Rna Polymerase ◽  
Transcription Elongation ◽  
Elongation Factors

scholarly journals HIV-1 Tat and Host AFF4 Recruit Two Transcription Elongation Factors into a Bifunctional Complex for Coordinated Activation of HIV-1 Transcription

2010 ◽  
Vol 38 (3) ◽  
pp. 428-438 ◽  
Author(s):  
Nanhai He ◽  
Min Liu ◽  
Joanne Hsu ◽  
Yuhua Xue ◽  
Seemay Chou ◽  
...  
Keyword(s):  
Transcription Elongation ◽  
Elongation Factors ◽  
Hiv 1

Structural insight into nucleosome transcription by RNA polymerase II with elongation factors

Science ◽  
2019 ◽  
Vol 363 (6428) ◽  
pp. 744-747 ◽  
Author(s):  
Haruhiko Ehara ◽  
Tomoya Kujirai ◽  
Yuka Fujino ◽  
Mikako Shirouzu ◽  
Hitoshi Kurumizaka ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Elongation ◽  
Chromosomal Dna ◽  
Elongation Factors ◽  
Cryo Electron Microscopy ◽  
Structural Insight ◽  
Insight Into

RNA polymerase II (RNAPII) transcribes chromosomal DNA that contains multiple nucleosomes. The nucleosome forms transcriptional barriers, and nucleosomal transcription requires several additional factors in vivo. We demonstrate that the transcription elongation factors Elf1 and Spt4/5 cooperatively lower the barriers and increase the RNAPII processivity in the nucleosome. The cryo–electron microscopy structures of the nucleosome-transcribing RNAPII elongation complexes (ECs) reveal that Elf1 and Spt4/5 reshape the EC downstream edge and intervene between RNAPII and the nucleosome. They facilitate RNAPII progression through superhelical location SHL(–1) by adjusting the nucleosome in favor of the forward progression. They suppress pausing at SHL(–5) by preventing the stable RNAPII-nucleosome interaction. Thus, the EC overcomes the nucleosomal barriers while providing a platform for various chromatin functions.

scholarly journals Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis inEscherichia coli

2012 ◽  
Vol 87 (2) ◽  
pp. 382-393 ◽  
Author(s):  
Mikhail Bubunenko ◽  
Donald L. Court ◽  
Abdalla Al Refaii ◽  
Shivalika Saxena ◽  
Alexey Korepanov ◽  
...  
Keyword(s):  
Transcription Elongation ◽  
Inescherichia Coli ◽  
Elongation Factors ◽  
Rnase Iii

scholarly journals Synthetic transcription elongation factors license transcription across repressive chromatin

Science ◽  
2017 ◽  
Vol 358 (6370) ◽  
pp. 1617-1622 ◽  
Author(s):  
Graham S. Erwin ◽  
Matthew P. Grieshop ◽  
Asfa Ali ◽  
Jun Qi ◽  
Matthew Lawlor ◽  
...  
Keyword(s):  
Transcription Elongation ◽  
Elongation Factors ◽  
Repressive Chromatin

scholarly journals Evidence that Spt2/Sin1, an HMG-Like Factor, Plays Roles in Transcription Elongation, Chromatin Structure, and Genome Stability in Saccharomyces cerevisiae

2006 ◽  
Vol 26 (4) ◽  
pp. 1496-1509 ◽  
Author(s):  
Amine Nourani ◽  
Francois Robert ◽  
Fred Winston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genome Stability ◽  
Transcription Elongation ◽  
Coding Region ◽  
Elongation Factors ◽  
Chromatin Dynamics ◽  
Coding Regions ◽  
Genome Wide ◽  
Genes Encoding ◽  
Approaches To Study

ABSTRACT Spt2/Sin1 is a DNA binding protein with HMG-like domains that has been suggested to play a role in chromatin-mediated transcription in Saccharomyces cerevisiae. Previous studies have suggested models in which Spt2 plays an inhibitory role in the initiation of transcription of certain genes. In this work, we have taken several approaches to study Spt2 in greater detail. Our results have identified previously unknown genetic interactions between spt2Δ and mutations in genes encoding transcription elongation factors, including members of the PAF and HIR/HPC complexes. In addition, genome-wide and gene-specific chromatin immunoprecipitation analyses suggest that Spt2 is primarily associated with coding regions in a transcription-dependent fashion. Furthermore, our results show that Spt2, like other elongation factors, is required for the repression of transcription from a cryptic promoter within a coding region and that Spt2 is also required for repression of recombination within transcribed regions. Finally, we provide evidence that Spt2 plays a role in regulating the levels of histone H3 over transcribed regions. Taken together, our results suggest a direct link for Spt2 with transcription elongation, chromatin dynamics, and genome stability.

scholarly journals Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae

2005 ◽  
Vol 25 (22) ◽  
pp. 10122-10135 ◽  
Author(s):  
Donald Prather ◽  
Nevan J. Krogan ◽  
Andrew Emili ◽  
Jack F. Greenblatt ◽  
Fred Winston
Keyword(s):  
Synthetic Lethality ◽  
Transcription Elongation ◽  
Elongation Factor ◽  
Regulation Of Transcription ◽  
Elongation Factors ◽  
Genome Wide ◽  
Genes Encoding ◽  
Active Transcription ◽  
Identification And Characterization

ABSTRACT In order to identify previously unknown transcription elongation factors, a genetic screen was carried out to identify mutations that cause lethality when combined with mutations in the genes encoding the elongation factors TFIIS and Spt6. This screen identified a mutation in YKL160W, hereafter named ELF1 (elongation factor 1). Further analysis identified synthetic lethality between an elf1Δ mutation and mutations in genes encoding several known elongation factors, including Spt4, Spt5, Spt6, and members of the Paf1 complex. Genome-wide synthetic lethality studies confirmed that elf1Δ specifically interacts with mutations in genes affecting transcription elongation. Chromatin immunoprecipitation experiments show that Elf1 is cotranscriptionally recruited over actively transcribed regions and that this association is partially dependent on Spt4 and Spt6. Analysis of elf1Δ mutants suggests a role for this factor in maintaining proper chromatin structure in regions of active transcription. Finally, purification of Elf1 suggests an association with casein kinase II, previously implicated in roles in transcription. Together, these results suggest an important role for Elf1 in the regulation of transcription elongation.

Sign in / Sign up

Export Citation Format

Share Document