scholarly journals Use of the Micronucleus Test on Tradescantia (Trad-MCN) to Evaluate the Genotoxic Effects of Air Pollution

10.5772/17959 ◽  
2011 ◽  
Author(s):  
Jose Roberto Cardoso Meireles ◽  
Eneida de Moraes Marcilio Cerqueir
Keyword(s):  
Air Pollution ◽  
Micronucleus Test ◽  
Genotoxic Effects

Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays

2007 ◽  
Vol 33 (7) ◽  
pp. 859-866 ◽  
Author(s):  
M. Škarek ◽  
J. Janošek ◽  
P. Čupr ◽  
J. Kohoutek ◽  
A. Novotná-Rychetská ◽  
...  
Keyword(s):  
Air Pollution ◽  
Genotoxic Effects ◽  
In Vitro Bioassays

scholarly journals Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo

2014 ◽  
Vol 50 (2) ◽  
pp. 251-256
Author(s):  
Igor Vivian de Almeida ◽  
Giovana Domingues ◽  
Lilian Capelari Soares ◽  
Elisângela Düsman ◽  
Veronica Elisa Pimenta Vicentini
Keyword(s):  
Rattus Norvegicus ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Term Treatment ◽  
Genotoxic Effects ◽  
Short Term ◽  
Short Term Treatment ◽  
Chromosomal Aberration Test

Flunitrazepam (FNZ) is a sedative benzodiazepine prescribed for the short-term treatment of insomnia. However, there are concerns regarding possible carcinogenic or genotoxic effects of this medicine. Thus, the aim of this study was to evaluate the cytotoxic, clastogenic and aneugenic effects of FNZ in hepatoma cells from Rattus norvegicus (HTC) in vitro and in bone marrow cells of Wistar rats in vivo. These effects were examined in vitro following treatment with 0.2, 1.0, 5.0 or 10 μg/mL FNZ using a micronucleus test with a cytokinesis block or in vivo using a chromosomal aberration test following treatment with 7, 15 or 30 μg/mL/kg body weight. The results showed that the benzodiazepine concentrations tested were not cytotoxic, aneugenic or clastogenic. However, considering the adverse effects of using this benzodiazepine, more studies are required.

scholarly journals Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2217
Author(s):  
Fany Renata Aguilera-Rodríguez ◽  
Ana Lourdes Zamora-Perez ◽  
Clara Luz Galván-Moreno ◽  
Rosalinda Gutiérrez-Hernández ◽  
Claudia Araceli Reyes Estrada ◽  
...  
Keyword(s):  
Murine Model ◽  
Leaf Extract ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Cytotoxic Effects ◽  
Blood Smears ◽  
Polychromatic Erythrocytes ◽  
Aqueous Leaf Extract ◽  
Peripheral Blood Smears ◽  
Sampling Times

Crataegus oxyacantha has been mainly used in traditional medicine for the treatment of cardiovascular diseases. However, its safety profile has not been fully established, since only the genotoxic effects of C. oxyacantha fruit have been described. Therefore, the objective of this work was evaluating the cytotoxicity and genotoxicity of the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha by means of the micronucleus test in a murine model. Doses of 2000, 1000, and 500 mg/kg of both extracts were administered orally for 5 days in mice of the Balb-C strain. Peripheral blood smears were performed at 0, 24, 48, 72, and 96 h after each administration. The number of polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MNPCEs), and micronucleated erythrocytes (MNEs) was determined at the different sampling times. Our results showed that the leaf and bark of C. oxyacantha increase the number of MNEs at the 2000 mg/kg dose, and only the aqueous leaf extract decreases the number of PCEs at the same dose. Therefore, the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha showed genotoxic effects, and only the aqueous leaf extract exhibited cytotoxic effects.

scholarly journals Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

2013 ◽  
Vol 2 (1) ◽  
pp. 26 ◽  
Author(s):  
Tânia Kawasaki de Araujo ◽  
Roseane Lopes da Silva-Grecco ◽  
Flora Margarida Barra Bisinotto ◽  
Nilson Camargos Roso ◽  
Cristina Wide Pissetti ◽  
...  
Keyword(s):  
Operating Room ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Operating Room Personnel

Evaluation of the genotoxic effects of Erythrina velutina (Fabaceae) using the in vivo micronucleus test

2018 ◽  
pp. 53-58
Author(s):  
B. Oliveira ◽  
F.S. Matos ◽  
D.S.B.S. Silva ◽  
B. Barboza ◽  
R. Scher ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Genotoxic Effects

scholarly journals In Vivo Evaluation of the Genotoxic Effects of Poly (Butylene adipate-co-terephthalate)/Polypyrrole with Nanohydroxyapatite Scaffolds for Bone Regeneration

Materials ◽  
2019 ◽  
Vol 12 (8) ◽  
pp. 1330 ◽  
Author(s):  
Conceição de Maria Vaz Elias ◽  
Antônio Luiz Martins Maia Filho ◽  
Laryssa Roque da Silva ◽  
Fabrício Pires de Moura do Amaral ◽  
Thomas J. Webster ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Infusion Pump ◽  
Tail Length ◽  
Positive Control ◽  
Negative Control ◽  
Electrospinning Process ◽  
Genotoxic Effects ◽  
In Vivo Evaluation ◽  
In Vivo Assays

Here, butylene adipate-co-terephthalate/polypyrrole with nanohydroxyapatite (PBAT/PPy/nHAp) scaffolds were fabricated and characterized. The electrospinning process was carried out using 12 kV, a needle of 23 G, an infusion pump set at 0.3 mL/h, and 10 cm of distance. Afterwards, nHAp was directly electrodeposited onto PBAT/PPy scaffolds using a classical three-electrode apparatus. For in vivo assays (comet assay, acute and chronic micronucleus), 60 male albino Wistar rats with 4 groups were used in each test (n = 5): PBAT/PPy; PBAT/PPy/nHAp; positive control (cyclophosphamide); and the negative control (distilled water). Peripheral blood samples were collected from the animals to perform the comet test after 4 h (for damage) and 24 h (for repair). In the comet test, it was shown that the scaffolds did not induce damage to the % DNA tail and neither for tail length. After the end of 48 h (for acute micronucleus) and 72 h (for chronic micronucleus), bone marrow was collected from each rat to perform the micronucleus test. All of the produced scaffolds did not present genotoxic effects, providing strong evidence for the biological application of PBAT/PPy/nHAp scaffolds.

scholarly journals Study of in vitro and in vivo genotoxic effects of air pollution fine (PM2.5-0.18) and quasi-ultrafine (PM0.18) particles on lung models

2020 ◽  
Vol 711 ◽  
pp. 134666 ◽  
Author(s):  
A. Platel ◽  
K. Privat ◽  
S. Talahari ◽  
A. Delobel ◽  
G. Dourdin ◽  
...  
Keyword(s):  
Air Pollution ◽  
Genotoxic Effects

scholarly journals Evaluation of Anti-Cytotoxic and Anti-Genotoxic Effects of Nigella sativa through a Micronucleus Test in BALB/c Mice

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1317
Author(s):  
Raúl S. Franco-Ramos ◽  
Carlos A. López-Romero ◽  
Hugo Torres-Ortega ◽  
Darío Oseguera-Herrera ◽  
Jose P. Lamoreaux-Aguayo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Single Dose ◽  
Micronucleus Test ◽  
Nigella Sativa ◽  
Pharmacological Effects ◽  
Sterile Water ◽  
Healthy Male ◽  
Genotoxic Effects ◽  
Similar Frequency ◽  
Polychromatic Erythrocytes

Nigella sativa (N. sativa) is a medicinal plant used for its therapeutic pharmacological effects such as anti-inflammatory, antioxidant, anticancer, antidiabetic, and immunomodulation. This study explored the anti-cytotoxic and anti-genotoxic effect of N. sativa through a micronucleus test (MNT) of BALB/c mice peripheral blood. Using 6-to-8-week-old healthy male BALB/c mice, four groups were formed: (1) Control (sterile water), single-dose 2 mg/kg/intraperitoneal (i.p); (2) N. sativa oil, 500 mg/kg/24 h/7 days/i.p; (3) Cisplatin (CP), single-dose 2 mg/kg/subcutaneous (s.c); (4) N. sativa + CP with their respective dosage. When evaluating polychromatic erythrocytes (PCE), a biomarker of cytotoxicity, the group treated with N. sativa + CP experienced an increase in the frequency of PCE, which demonstrated the recovery of bone marrow and modulation of cell proliferation. The analysis of micronucleated polychromatic erythrocytes (MNPCE), an acute genotoxicity biomarker, showed similar frequency of MNPCE within the groups except in CP, but, in the N. sativa + CP group, the frequency of MNPCE decreased and then regulated. Finally, the frequency of micronucleated erythrocytes (MNE), a biomarker of genotoxicity, the supplementation of N. sativa oil did not induce genotoxic damage in this model. Thus, we conclude that N. sativa has both cytoprotective, genoprotective effects and modulates cell proliferation in BALB/c mice.

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