scholarly journals Evaluation of Anti-Cytotoxic and Anti-Genotoxic Effects of Nigella sativa through a Micronucleus Test in BALB/c Mice

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1317
Author(s):  
Raúl S. Franco-Ramos ◽  
Carlos A. López-Romero ◽  
Hugo Torres-Ortega ◽  
Darío Oseguera-Herrera ◽  
Jose P. Lamoreaux-Aguayo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Single Dose ◽  
Micronucleus Test ◽  
Nigella Sativa ◽  
Pharmacological Effects ◽  
Sterile Water ◽  
Healthy Male ◽  
Genotoxic Effects ◽  
Similar Frequency ◽  
Polychromatic Erythrocytes

Nigella sativa (N. sativa) is a medicinal plant used for its therapeutic pharmacological effects such as anti-inflammatory, antioxidant, anticancer, antidiabetic, and immunomodulation. This study explored the anti-cytotoxic and anti-genotoxic effect of N. sativa through a micronucleus test (MNT) of BALB/c mice peripheral blood. Using 6-to-8-week-old healthy male BALB/c mice, four groups were formed: (1) Control (sterile water), single-dose 2 mg/kg/intraperitoneal (i.p); (2) N. sativa oil, 500 mg/kg/24 h/7 days/i.p; (3) Cisplatin (CP), single-dose 2 mg/kg/subcutaneous (s.c); (4) N. sativa + CP with their respective dosage. When evaluating polychromatic erythrocytes (PCE), a biomarker of cytotoxicity, the group treated with N. sativa + CP experienced an increase in the frequency of PCE, which demonstrated the recovery of bone marrow and modulation of cell proliferation. The analysis of micronucleated polychromatic erythrocytes (MNPCE), an acute genotoxicity biomarker, showed similar frequency of MNPCE within the groups except in CP, but, in the N. sativa + CP group, the frequency of MNPCE decreased and then regulated. Finally, the frequency of micronucleated erythrocytes (MNE), a biomarker of genotoxicity, the supplementation of N. sativa oil did not induce genotoxic damage in this model. Thus, we conclude that N. sativa has both cytoprotective, genoprotective effects and modulates cell proliferation in BALB/c mice.

scholarly journals Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2217
Author(s):  
Fany Renata Aguilera-Rodríguez ◽  
Ana Lourdes Zamora-Perez ◽  
Clara Luz Galván-Moreno ◽  
Rosalinda Gutiérrez-Hernández ◽  
Claudia Araceli Reyes Estrada ◽  
...  
Keyword(s):  
Murine Model ◽  
Leaf Extract ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Cytotoxic Effects ◽  
Blood Smears ◽  
Polychromatic Erythrocytes ◽  
Aqueous Leaf Extract ◽  
Peripheral Blood Smears ◽  
Sampling Times

Crataegus oxyacantha has been mainly used in traditional medicine for the treatment of cardiovascular diseases. However, its safety profile has not been fully established, since only the genotoxic effects of C. oxyacantha fruit have been described. Therefore, the objective of this work was evaluating the cytotoxicity and genotoxicity of the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha by means of the micronucleus test in a murine model. Doses of 2000, 1000, and 500 mg/kg of both extracts were administered orally for 5 days in mice of the Balb-C strain. Peripheral blood smears were performed at 0, 24, 48, 72, and 96 h after each administration. The number of polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MNPCEs), and micronucleated erythrocytes (MNEs) was determined at the different sampling times. Our results showed that the leaf and bark of C. oxyacantha increase the number of MNEs at the 2000 mg/kg dose, and only the aqueous leaf extract decreases the number of PCEs at the same dose. Therefore, the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha showed genotoxic effects, and only the aqueous leaf extract exhibited cytotoxic effects.

PHENYTOIN SODIUM INDUCED MICRONUCLEI IN THE POLYCHROMATIC ERYTHROCYTES OF CD-1 MOUSE PERIPHERAL BLOOD

2018 ◽  
Vol 11 (41-42) ◽  
Author(s):  
Norberto Alarcón-Herrera ◽  
Saúl Flores-Maya
Keyword(s):  
Mus Musculus ◽  
Peripheral Blood ◽  
Seizure Control ◽  
Cell Kinetics ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Polychromatic Erythrocytes ◽  
Phenytoin Sodium ◽  
Neural Disorders ◽  
Higher Doses

Seizures are one of the most common neural disorders, and may be sporadic or recurrent (epilepsy) crisis. In the case of people with epilepsy, these must be treated throughout their lives, being a major phenytoin prescription for seizure control medicines out. However, despite having several decades in the market there is very little information about their genotoxic effects. Therefore in this study to evaluate the ability of phenytoin to induce genotoxic damage for 30 days using the micronucleus test in mice <em>Mus musculus</em> CD-1 strain. It was determined that the three doses of phenytoin used (2.8, 4.2 and 6.64 mg/kg) induced clastogenicity in mouse chromosomes, that at higher doses the damage is greater. Furthermore, also inhibiting cytotoxic damage induced cell kinetics for doses of 4.2 and 6.64 mg/kg.

Studies of the Toxicological Potential of Capsinoids: VI. Single-Dose Toxicity Study and Micronucleus Test of Commercial-Grade Dihydrocapsiate

2008 ◽  
Vol 27 (3_suppl) ◽  
pp. 73-77 ◽  
Author(s):  
Eri Watanabe ◽  
Terutaka Kodama ◽  
Takeshi Masuyama ◽  
Shoji Tsubuku ◽  
Madoka Nakajima ◽  
...  
Keyword(s):  
Single Dose ◽  
Micronucleus Test ◽  
Observation Interval ◽  
Lethal Dose ◽  
Toxicity Study ◽  
Spontaneous Movement ◽  
Oral Toxicity ◽  
Commercial Grade ◽  
Polychromatic Erythrocytes

A single-dose oral toxicity study was conducted to examine the qualitative and quantitative toxicity of a commercial-grade batch of dihydrocapsiate (4-hydroxy-3-methoxybenzyl 8-methylnonanoate; CAS No. 205687-03-2). Dihydrocapsiate was administered once by gavage to ICR mice at dose levels of 0 (vehicle) or 5000 mg/kg/day. No mortality was observed during the 14 day observation period following test article administration. During the 2 h immediately following dosing, mice of both sexes treated with dihydrocapsiate were observed to exhibit one or more of the following: staggered gait, decreased spontaneous movement, increased time in the prone position, tremors, gasping, or red-brownish urine. All mice had completely recovered by the 6 h observation interval. No effects on body weights or necropsy findings were observed as a result of dihydrocapsiate administration. These results suggested that the lethal dose of dihydrocapsiate was >5000 mg/kg. In an in vivo micronucleus test using BDF1 male mice, a commercial grade of dihydrocapsiate neither increased the incidence of micronucleated polychromatic erythrocytes (MNPCEs) nor decreased the ratio of polychromatic erythrocytes (PCEs) in any of the treatment groups. The results suggest that commercial-grade dihydrocapsiate is unlikely to be an in vivo clastogen.

scholarly journals Evaluation of (anti)genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test

2013 ◽  
Vol 49 (1) ◽  
pp. 135-148 ◽  
Author(s):  
Fernando Márlisson de Queiroz ◽  
Kayo Wanderson de Oliveira Matias ◽  
Mylena Mylana Freire da Cunha ◽  
Aline Schwarz
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Weight Gain ◽  
Micronucleus Test ◽  
Body Weight Gain ◽  
Tap Water ◽  
Control Group ◽  
Cytotoxic Activities ◽  
Phyllanthus Niruri ◽  
Polychromatic Erythrocytes

Phyllanthus niruri L. (Euphorbiaceae), known as "quebra-pedra" (Portuguese for "stonebreaker"), is an herb used for kidney disorders. In light of its frequent use by the population, the present study aimed to investigate the genotoxic, antigenotoxic and cytotoxic activities of a standardized P. niruri extract in bone marrow rats. Three groups of 12 animals were treated daily by gavage over a period of 30 days, with 50, 150 or 250 mg/kg of P. niruri extract aqueous solution. The control group (n = 12) received tap water. At the end of treatment (day 31), groups were divided into two minor subgroups (n=6/group) and received cyclophosphamide (50 mg/kg, i.p.) or saline 0.9% (i.p.). After 24 hours, we evaluated the frequency of micronucleated polychromatic erythrocytes for each animal (MNPCE) at 1000 PCE. Cytotoxicity was evaluated with the PCE/NCE ratio (NEC = normochromatic erythrocytes). General toxicity was assessed during treatment using the parameters of body weight gain, ration and water consumption. The dry extract did not provoke changes in body weight, weight gain, ration and water intake or changes in the frequency of MNPCE or cytotoxicity in bone marrow. We propose that the P. niruri extract used here showed no genotoxic, antigenotoxic and cytotoxic activities under the experimental conditions.

scholarly journals Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo

2014 ◽  
Vol 50 (2) ◽  
pp. 251-256
Author(s):  
Igor Vivian de Almeida ◽  
Giovana Domingues ◽  
Lilian Capelari Soares ◽  
Elisângela Düsman ◽  
Veronica Elisa Pimenta Vicentini
Keyword(s):  
Rattus Norvegicus ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Term Treatment ◽  
Genotoxic Effects ◽  
Short Term ◽  
Short Term Treatment ◽  
Chromosomal Aberration Test

Flunitrazepam (FNZ) is a sedative benzodiazepine prescribed for the short-term treatment of insomnia. However, there are concerns regarding possible carcinogenic or genotoxic effects of this medicine. Thus, the aim of this study was to evaluate the cytotoxic, clastogenic and aneugenic effects of FNZ in hepatoma cells from Rattus norvegicus (HTC) in vitro and in bone marrow cells of Wistar rats in vivo. These effects were examined in vitro following treatment with 0.2, 1.0, 5.0 or 10 μg/mL FNZ using a micronucleus test with a cytokinesis block or in vivo using a chromosomal aberration test following treatment with 7, 15 or 30 μg/mL/kg body weight. The results showed that the benzodiazepine concentrations tested were not cytotoxic, aneugenic or clastogenic. However, considering the adverse effects of using this benzodiazepine, more studies are required.

scholarly journals Study of the correlation between blood cholinesterases activity, urinary dialkyl phosphates, and the frequency of micronucleated polychromatic erythrocytes in rats exposed to disulfoton

2013 ◽  
Vol 49 (1) ◽  
pp. 149-154 ◽  
Author(s):  
Mariane Gonçalves Santos ◽  
Ricardo Vilela Vitor ◽  
Maurício Gustavo Nakamura ◽  
Luana de Souza Morelini ◽  
Rafaela Scalco Ferreira ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Pesticide Exposure ◽  
Micronucleus Test ◽  
Urinary Metabolites ◽  
Chromosomal Damage ◽  
Polychromatic Erythrocytes ◽  
Induce Mutagenesis ◽  
Diethyl Dithiophosphate ◽  
Bone Marrow Micronucleus

Organophosphates (OPs) are widely used as pesticides, and its urinary metabolites as well as the blood cholinesterases (ChEs) activity have been reported as possible biomarkers for the assessment of this pesticide exposure. Moreover, the OPs can induce mutagenesis, and the bone marrow micronucleus test is an efficient way to assess this chromosomal damage. This paper reports a study carried out to verify the correlation among the disulfoton exposure, blood ChEs activity, urinary diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP), as well as micronucleated polychromatic erythrocytes (MNPCEs) frequency. Four groups of rats (n=12) were exposed to disulfoton at 0, 2.8, 4.7, and 6.6 mg kg-1 body weight. The blood ChEs activity, urinary DETP and DEDTP concentrations, and MNPCEs frequency were determined. It was observed that the plasmatic and erythrocytary ChEs activity decreased from 2.9% to 0.5% and from 35.9 to 3.3%, respectively, when the disulfoton dose was increased from 0 to 6.6 mg kg-1 (correlation of 0.99). Urinary DETP and DEDTP concentrations, as well as the MNPCEs frequency, increased from 0 to 6.58 µg mL-1, from 0 to 0.04 µg mL-1, and from 0 to 1.4%, respectively, when the disulfoton dose was increased from 0 to 6.58 mg kg-1 body weight.

Suppression of Mesangial-Cell Proliferation by Trapidil in Glomerulonephritis Induced by Anti-Thymocyte Serum in Rats

1995 ◽  
Vol 23 (6) ◽  
pp. 458-466 ◽  
Author(s):  
M S Razzaque ◽  
M Cheng ◽  
T Taguchi
Keyword(s):  
Cell Proliferation ◽  
Body Weight ◽  
Single Dose ◽  
Growth Factor ◽  
Mesangial Cell ◽  
Platelet Derived Growth Factor ◽  
Group Iii ◽  
Group I ◽  
Mesangial Cell Proliferation ◽  
Group Ii

Trapadil (Mochida Pharmaceuticals, Japan), an antiplatelet drug, suppresses the growth of several cell types and is thought to antagonize platelet-derived growth factor. The effects of trapidil on mesangial-cell proliferation in glomerulonephritis induced by anti-thymocyte serum in Wistar rats were investigated. Control rats were treated with phosphate-buffered saline (group I); group II rats were injected with a single dose of anti-thymocyte serum (8 ml/kg body weight), and group III rats were treated with both a single dose of anti-thymocyte serum (8 ml/kg body weight) and with trapidil (5 mg/kg body weight/day). Three rats in each group were killed on day 3, and the other three on day 10. Control rats showed no significant histological changes on day 3 or day 10. In group II, on day 3, there was a marked decrease in glomerular cell numbers, with mesangiolysis. Histologically severe mesangial-cell proliferation with expansion of mesangial areas was noted on day 10. None of the rats in group III showed mesangial alterations, histologically, indicating that mesangial-cell proliferation was suppressed by trapidil. This suppression may result from antagonism of the binding of platelet derived growth factor to the specific surface receptors in the mesangial cells. Trapidil may have clinical value in the treatment of mesangial-cell proliferative glomerular diseases.

Biological response genes after single dose administration of interferon β-1b to healthy male volunteers

2008 ◽  
Vol 199 (1-2) ◽  
pp. 115-125 ◽  
Author(s):  
Jan Hilpert ◽  
Johanna M. Beekman ◽  
Susanne Schwenke ◽  
Kristin Kowal ◽  
David Bauer ◽  
...  
Keyword(s):  
Single Dose ◽  
Biological Response ◽  
Healthy Male ◽  
Interferon Β ◽  
Dose Administration ◽  
Single Dose Administration ◽  
Healthy Male Volunteers ◽  
Male Volunteers

scholarly journals Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

2013 ◽  
Vol 2 (1) ◽  
pp. 26 ◽  
Author(s):  
Tânia Kawasaki de Araujo ◽  
Roseane Lopes da Silva-Grecco ◽  
Flora Margarida Barra Bisinotto ◽  
Nilson Camargos Roso ◽  
Cristina Wide Pissetti ◽  
...  
Keyword(s):  
Operating Room ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Operating Room Personnel
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