scholarly journals Orchidectomy Upregulates While Testosterone Treatment Downregulates the Expression of Ornithine Aminotransferase Gene in the Mouse Kidney

10.5772/16583 ◽  
2011 ◽  
Author(s):  
Olivier Levillain ◽  
Cyril Degletagne ◽  
Dominique Letexier ◽  
Henri Dechau
Keyword(s):  
Mouse Kidney ◽  
Ornithine Aminotransferase ◽  
Testosterone Treatment ◽  
Ornithine Aminotransferase Gene

Heterogenous expression of ornithine decarboxylase gene in the proximal tubule of the mouse kidney following testosterone treatment

1993 ◽  
Vol 100 (5) ◽  
pp. 325-330 ◽  
Author(s):  
Noriyuki Koibuchi ◽  
Shigeru Matsuzaki ◽  
Mikako Sakai ◽  
Hideki Ohtake ◽  
Sadao Yamaoka
Keyword(s):  
Proximal Tubule ◽  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Testosterone Treatment

Sex-differential expression of ornithine aminotransferase in the mouse kidney

2007 ◽  
Vol 292 (3) ◽  
pp. F1016-F1027 ◽  
Author(s):  
Olivier Levillain ◽  
Gabrielle Ventura ◽  
Henri Déchaud ◽  
Maya Hobeika ◽  
Anna Meseguer ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Differential Expression ◽  
Mouse Kidney ◽  
Plasma Testosterone ◽  
Ornithine Aminotransferase ◽  
Male And Female ◽  
Protein Levels ◽  
Ovariectomized Mice ◽  
Specific Distribution ◽  
Chain Acyl

The mouse kidney expresses the gene of ornithine aminotransferase ( Oat). Previous works suggest that Oat is differentially expressed in female and male mouse kidney (Alonso E, Rubio V. Biochem J 259: 131–138, 1989; Levillain O, Diaz JJ, Blanchard O, Dechaud H. Endocrinology 146: 950–959, 2005; Manteuffel-Cymborowska M, Chmurzynska W, Peska M, Grzelakowska-Sztabert B. Int J Biochem Cell Biol 27: 287–295, 1995; Natesan S, Reddy SR. Comp Biochem Physiol B Biochem Mol Biol 130: 585–595, 2001; Yu H, Yoo PK, Aguirre CC, Tsoa RW, Kern RM, Grody WW, Cederbaum SD, Iyer RK. J Histochem Cytochem 51: 1151–1160, 2003). This study was designed to provide a detailed description of the sexual dimorphism of Oat expression in the mouse kidney and to test the influence of sex hormones on its regulation. Experiments were performed on male and female Swiss OF1 mice during their postnatal development, at adulthood, and in orchidectomized and ovariectomized mice. Kidneys, dissected renal zones, and mitochondria were used to analyze OAT mRNA and protein levels and measure OAT activity. The results revealed that before puberty, Oat expression was similar between female and male kidneys whereas from puberty until adulthood Oat expression increased in the female kidney, becoming ≈2.5-fold higher than in the male kidney. This sex-differential expression of Oat was associated with a sex-specific distribution of Oat along the corticopapillary axis and within the nephron. OAT was three- to fourfold more expressed in the female than the male cortex. In males, Oat was highly expressed in the medulla, mainly in the thick ascending limbs. Renal Oat distribution in orchidectomized mice resembled that in the females. Ovariectomy did not influence Oat expression. Sex differences are explained by the physiological increase in plasma testosterone in males. Expression of medium-chain acyl-CoA synthetase protein confirmed this finding. We report sexual dimorphism of Oat expression in the mouse kidney and show that Oat is naturally downregulated in the presence of testosterone.

Induction of ornithine decarboxylase immunoreactivity in the male mouse kidney following testosterone treatment: an axial heterogeneity in the proximal tubule

1993 ◽  
Vol 136 (1) ◽  
pp. 85-NP ◽  
Author(s):  
N. Koibuchi ◽  
S. Matsuzaki ◽  
H.-T. Ma ◽  
M. Sakai ◽  
S. Yamaoka
Keyword(s):  
Proximal Tubule ◽  
Ornithine Decarboxylase ◽  
Protein Level ◽  
Male Mouse ◽  
Subcutaneous Injection ◽  
Mouse Kidney ◽  
Luminal Surface ◽  
Testosterone Treatment ◽  
Pars Recta ◽  
Pars Convoluta

ABSTRACT The effect of testosterone on the activity of ornithine decarboxylase (ODC), its protein level and immunocytochemical distribution were examined in the mouse kidney. Male BALB C mice at 8 weeks of age were used throughout. Fourteen hours before death, they received a subcutaneous injection of testosterone (1 mg/animal) or solvent to measure renal ODC activity or to detect the distribution of ODC immunoreactivity in the kidney. Renal ODC activity and the content of the enzyme were markedly increased after testosterone treatment. Histologically, few cells that were obviously immunoreactive to ODC were observed in the control animals and in the testosterone-treated animals a marked increase in ODC immunoreactivity was observed only in the cortex. ODC immunoreactive cells were located diffusely in the proximal tubule. In the pars recta, cells were stained weakly and homogeneously, while in the pars convoluta, the luminal surface of the cells showed stronger immunoreactivity. Moreover, many granule-like particles that were strongly ODC immunoreactive were observed inside the lumen of the pars convoluta. These results show that testosterone treatment induces an increase in ODC content in certain cells located in the proximal tubule of the cortex. Journal of Endocrinology (1993) 136, 85–89

scholarly journals Mono- and poly-ADP-ribosylation of proteins in mouse kidney after castration and testosterone treatment

1981 ◽  
Vol 198 (1) ◽  
pp. 37-44 ◽  
Author(s):  
A Gartemann ◽  
R Bredehorst ◽  
K Wielckens ◽  
W H Strätling ◽  
H Hilz
Keyword(s):  
Enzyme Activity ◽  
Total Protein ◽  
Nuclear Protein ◽  
Mouse Kidney ◽  
Testosterone Treatment ◽  
Control Value ◽  
Protein Conjugates ◽  
Testosterone Substitution ◽  
Testosterone Administration ◽  
Adp Ribosyltransferase

Protein-bound mono(ADP-ribose) and poly(ADP-ribose) residues were determined in mouse kidney after castration and testosterone substitution. After these treatments, the mouse kidney undergoes significant alterations in the extent and pattern of transcription without changes in the amount of DNA and nuclear protein. The amount of mono(ADP-ribose)-protein conjugates (the hydroxylamine-sensitive and -resistant subfractions) decreased by 40% after castration, and returned to normal within 1 week after daily testosterone injections. Polymeric ADP-ribose residues, which amounted to less than 0.3% of the total protein-bound monomeric ADP-ribose, increased after castration and rapidly decreased on testosterone administration. The magnitude of these effects indicates that the decrease in mono(ADP-ribose) was not caused by a shift of monomeric residues into the polymer form. Nuclear ADP-ribosyltransferase activity showed a retarded decrease after castration, reaching 60% of the control value by day 20. After testosterone injections, enzyme activity rose to normal within 3-4 days. The amounts of the substrate NAD+ as well as of NAD+ + NADH also declined after castration, and rapidly returned to values slightly above normal when the androgen was substituted. The differential response of monomeric and polymeric ADP-ribose residues to castration and testosterone treatment suggests that the two modifications serve different functions.

Mapping of ornithine aminotransferase gene sequences to mouse Chromosomes 7, X, and 3

1992 ◽  
Vol 3 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Vijaya Ramesh ◽  
Shirley V. Cheng ◽  
Christine A. Kozak ◽  
Bruce J. Herron ◽  
Vivian E. Shih ◽  
...  
Keyword(s):  
Ornithine Aminotransferase ◽  
Gene Sequences ◽  
Ornithine Aminotransferase Gene

scholarly journals A deletion in the ornithine aminotransferase gene in gyrate atrophy.

1992 ◽  
Vol 267 (18) ◽  
pp. 12950-12954
Author(s):  
Y Akaki ◽  
Y Hotta ◽  
Y Mashima ◽  
A Murakami ◽  
N.G. Kennaway ◽  
...  
Keyword(s):  
Ornithine Aminotransferase ◽  
Gyrate Atrophy ◽  
Ornithine Aminotransferase Gene
Sign in / Sign up

Export Citation Format

Share Document