Heterogenous expression of ornithine decarboxylase gene in the proximal tubule of the mouse kidney following testosterone treatment

1993 ◽  
Vol 100 (5) ◽  
pp. 325-330 ◽  
Author(s):  
Noriyuki Koibuchi ◽  
Shigeru Matsuzaki ◽  
Mikako Sakai ◽  
Hideki Ohtake ◽  
Sadao Yamaoka
Keyword(s):  
Proximal Tubule ◽  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Testosterone Treatment

Induction of ornithine decarboxylase immunoreactivity in the male mouse kidney following testosterone treatment: an axial heterogeneity in the proximal tubule

1993 ◽  
Vol 136 (1) ◽  
pp. 85-NP ◽  
Author(s):  
N. Koibuchi ◽  
S. Matsuzaki ◽  
H.-T. Ma ◽  
M. Sakai ◽  
S. Yamaoka
Keyword(s):  
Proximal Tubule ◽  
Ornithine Decarboxylase ◽  
Protein Level ◽  
Male Mouse ◽  
Subcutaneous Injection ◽  
Mouse Kidney ◽  
Luminal Surface ◽  
Testosterone Treatment ◽  
Pars Recta ◽  
Pars Convoluta

ABSTRACT The effect of testosterone on the activity of ornithine decarboxylase (ODC), its protein level and immunocytochemical distribution were examined in the mouse kidney. Male BALB C mice at 8 weeks of age were used throughout. Fourteen hours before death, they received a subcutaneous injection of testosterone (1 mg/animal) or solvent to measure renal ODC activity or to detect the distribution of ODC immunoreactivity in the kidney. Renal ODC activity and the content of the enzyme were markedly increased after testosterone treatment. Histologically, few cells that were obviously immunoreactive to ODC were observed in the control animals and in the testosterone-treated animals a marked increase in ODC immunoreactivity was observed only in the cortex. ODC immunoreactive cells were located diffusely in the proximal tubule. In the pars recta, cells were stained weakly and homogeneously, while in the pars convoluta, the luminal surface of the cells showed stronger immunoreactivity. Moreover, many granule-like particles that were strongly ODC immunoreactive were observed inside the lumen of the pars convoluta. These results show that testosterone treatment induces an increase in ODC content in certain cells located in the proximal tubule of the cortex. Journal of Endocrinology (1993) 136, 85–89

Investigation of structure and rate of synthesis of ornithine decarboxylase protein in mouse kidney

Biochemistry ◽  
1984 ◽  
Vol 23 (16) ◽  
pp. 3777-3783 ◽  
Author(s):  
Lo Persson ◽  
James E. Seely ◽  
Anthony E. Pegg
Keyword(s):  
Ornithine Decarboxylase ◽  
Mouse Kidney

scholarly journals Studies of mammalian ornithine decarboxylase using a monoclonal antibody

1984 ◽  
Vol 217 (1) ◽  
pp. 123-128 ◽  
Author(s):  
A E Pegg ◽  
J E Seely ◽  
L Persson ◽  
M Herlyn ◽  
K Ponsell ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ornithine Decarboxylase ◽  
Mouse Kidney ◽  
Immunoglobulin M ◽  
Active Enzyme ◽  
Sodium Thiocyanate ◽  
Immunoaffinity Column ◽  
Rapid Turnover ◽  
Cytosolic Protein

A monoclonal antibody of the immunoglobulin M class was produced against mouse kidney ornithine decarboxylase. Screening for the antibody was carried out using alpha-difluoromethyl[5-3H]ornithine-labelled ornithine decarboxylase. The antibody reacted with this antigen and with native ornithine decarboxylase. The antibody attached to Sepharose could be used to form an immunoaffinity column that retained mammalian ornithine decarboxylase. The active enzyme could then be eluted in a highly purified form by 1.0M-sodium thiocyanate. The monoclonal antibody could also be used to precipitate labelled ornithine decarboxylase from homogenates of kidneys from androgen-treated mice given [35S]methionine. Only one band, corresponding to Mr of about 55000, was observed. The extensive labelling of this band is consistent with the rapid turnover of ornithine decarboxylase protein, since this enzyme represents only about 1 part in 10000 of the cytosolic protein.

scholarly journals Enzymatic histochemistry of mouse kidney in plastic.

1987 ◽  
Vol 35 (4) ◽  
pp. 483-487 ◽  
Author(s):  
T P Pretlow ◽  
A S Lapinsky ◽  
L C Flowers ◽  
R W Grane ◽  
T G Pretlow
Keyword(s):  
Alkaline Phosphatase ◽  
Proximal Tubule ◽  
Leucine Aminopeptidase ◽  
Kidney Diseases ◽  
Mouse Kidney ◽  
Frozen Sections ◽  
Gamma Glutamyl Transpeptidase ◽  
Glutamyl Transpeptidase ◽  
Bowman's Capsule

Two-micrometer sections of methacrylate-embedded kidney were used to investigate the enzymatic activities of mouse kidney where the proximal tubule and Bowman's capsule from the same corpuscle were viewed in the same section. Alkaline phosphatase, acid phosphatase, 5'-nucleotidase, gamma-glutamyl transpeptidase, N-acetyl-beta-glucosaminidase, leucine aminopeptidase, alpha-naphthyl butyrate esterase, and adenosine triphosphatase activities were observed in the proximal tubule, but only 5'-nucleotidase, alpha-naphthyl butyrate esterase, and alkaline phosphatase were observed in the squamous portion of the parietal epithelium of Bowman's capsule. The use of methacrylate-embedded tissue allowed more precise localization of enzymatic activity than is possible with most frozen sections. This may provide interesting applications not only for characterization of kidney diseases but also for characterization of other normal and abnormal tissues.

Involvement of the MAPK-kinase pathway in the PTH-mediated regulation of the proximal tubule type IIa Na+/Pi cotransporter in mouse kidney

2003 ◽  
Vol 446 (1) ◽  
pp. 52-60 ◽  
Author(s):  
Desa Bacic ◽  
Nicole Schulz ◽  
Jürg Biber ◽  
Brigitte Kaissling ◽  
Heini Murer ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Mouse Kidney ◽  
Mapk Kinase ◽  
Kinase Pathway

A hypertonicity-activated nonselective conductance in single proximal tubule cells isolated from mouse kidney

2003 ◽  
Vol 192 (3) ◽  
pp. 191-201 ◽  
Author(s):  
K. J. D. Balloch ◽  
J. A. Hartley ◽  
I. D. Millar ◽  
J. D. Kibble ◽  
L. Robson
Keyword(s):  
Proximal Tubule ◽  
Mouse Kidney ◽  
Proximal Tubule Cells ◽  
Tubule Cells
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