scholarly journals Hematopoietic Differentiation from Embryonic Stem Cells

10.5772/16086 ◽  
2011 ◽  
Author(s):  
Yasuhisa Yokoyama ◽  
Hidekazu Nishikii ◽  
Shigeru Chib
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation

scholarly journals In vitro hematopoietic differentiation of mouse embryonic stem cells requires the tumor suppressor menin and is mediated by Hoxa9

2009 ◽  
Vol 126 (7) ◽  
pp. 517-522 ◽  
Author(s):  
Elizabeth Novotny ◽  
Sheila Compton ◽  
P. Paul Liu ◽  
Francis S. Collins ◽  
Settara C. Chandrasekharappa
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Tumor Suppressor ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Hematopoietic Differentiation

Cells Extract from Fetal Liver Promotes the Hematopoietic Differentiation of Human Embryonic Stem Cells

2009 ◽  
Vol 11 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Yu-Xiao Liu ◽  
Lei Ji ◽  
Wen Yue ◽  
Zhi-Feng Yan ◽  
Jing Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Fetal Liver ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation

scholarly journals RUNX1a enhances hematopoietic lineage commitment from human embryonic stem cells and inducible pluripotent stem cells

Blood ◽  
2013 ◽  
Vol 121 (15) ◽  
pp. 2882-2890 ◽  
Author(s):  
Dan Ran ◽  
Wei-Jong Shia ◽  
Miao-Chia Lo ◽  
Jun-Bao Fan ◽  
David A. Knorr ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Ex Vivo ◽  
Ectopic Expression ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Lineage Commitment ◽  
Hematopoietic Differentiation

Abstract Advancements in human pluripotent stem cell (hPSC) research have potential to revolutionize therapeutic transplantation. It has been demonstrated that transcription factors may play key roles in regulating maintenance, expansion, and differentiation of hPSCs. In addition to its regulatory functions in hematopoiesis and blood-related disorders, the transcription factor RUNX1 is also required for the formation of definitive blood stem cells. In this study, we demonstrated that expression of endogenous RUNX1a, an isoform of RUNX1, parallels with lineage commitment and hematopoietic emergence from hPSCs, including both human embryonic stem cells and inducible pluripotent stem cells. In a defined hematopoietic differentiation system, ectopic expression of RUNX1a facilitates emergence of hematopoietic progenitor cells (HPCs) and positively regulates expression of mesoderm and hematopoietic differentiation-related factors, including Brachyury, KDR, SCL, GATA2, and PU.1. HPCs derived from RUNX1a hPSCs show enhanced expansion ability, and the ex vivo–expanded cells are capable of differentiating into multiple lineages. Expression of RUNX1a in embryoid bodies (EBs) promotes definitive hematopoiesis that generates erythrocytes with β-globin production. Moreover, HPCs generated from RUNX1a EBs possess ≥9-week repopulation ability and show multilineage hematopoietic reconstitution in vivo. Together, our results suggest that RUNX1a facilitates the process of producing therapeutic HPCs from hPSCs.

scholarly journals Hematopoietic Differentiation of Embryonic Stem Cells: An In Vitro Model to Study Gene Regulation during Megakaryocytopoiesis

Stem Cells ◽  
1996 ◽  
Vol 14 (S1) ◽  
pp. 194-199 ◽  
Author(s):  
Georges Uzan ◽  
Marie‐Hélène Prandini ◽  
Jean‐Philippe Rosa ◽  
Rolande Berthier
Keyword(s):  
Stem Cells ◽  
Gene Regulation ◽  
Embryonic Stem Cells ◽  
In Vitro Model ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation ◽  
Vitro Model ◽  
Study Gene Regulation

scholarly journals OP9 Stroma Augments Survival of Hematopoietic Precursors and Progenitors During Hematopoietic Differentiation from Human Embryonic Stem Cells

Stem Cells ◽  
2008 ◽  
Vol 26 (12) ◽  
pp. 3267-3267
Author(s):  
Junfeng Ji ◽  
Kausalia Vijayaragavan ◽  
Marc Bosse ◽  
Pablo Menendez ◽  
Katja Weisel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation

scholarly journals Quantitative Oct4 Overproduction in Mouse Embryonic Stem Cells Results in Prolonged Mesoderm Commitment During Hematopoietic Differentiation In Vitro

Stem Cells ◽  
2006 ◽  
Vol 24 (8) ◽  
pp. 1937-1945 ◽  
Author(s):  
Valérie Camara-Clayette ◽  
Françoise Le Pesteur ◽  
William Vainchenker ◽  
Françoise Sainteny
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Hematopoietic Differentiation

scholarly journals Dysregulated Gene Expression During Hematopoietic Differentiation From Human Embryonic Stem Cells

2011 ◽  
Vol 19 (4) ◽  
pp. 768-781 ◽  
Author(s):  
Gautam Dravid ◽  
Yuhua Zhu ◽  
Jessica Scholes ◽  
Denis Evseenko ◽  
Gay M Crooks
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation
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