scholarly journals Association of DNA Methylation Levels with Tissue-specific Expression of Adipogenic and Lipogenic Genes in Longissimus dorsi Muscle of Korean Cattle

2014 ◽  
Vol 27 (10) ◽  
pp. 1493-1498 ◽  
Author(s):  
M. Baik ◽  
T. T. T. Vu ◽  
M. Y. Piao ◽  
H. J. Kang
Keyword(s):  
Dna Methylation ◽  
Longissimus Dorsi ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Korean Cattle ◽  
Longissimus Dorsi Muscle ◽  
Lipogenic Genes ◽  
Dorsi Muscle

scholarly journals DNA Methylation of Alternative Promoters Directs Tissue Specific Expression of Epac2 Isoforms

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e67925 ◽  
Author(s):  
Erling A. Hoivik ◽  
Solveig L. Witsoe ◽  
Inger R. Bergheim ◽  
Yunjian Xu ◽  
Ida Jakobsson ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Alternative Promoters ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Bridging sequence diversity and tissue-specific expression by DNA methylation in genes of the mouse prolactin superfamily

2011 ◽  
Vol 23 (5-6) ◽  
pp. 336-345 ◽  
Author(s):  
Koji Hayakawa ◽  
Momo O. Nakanishi ◽  
Jun Ohgane ◽  
Satoshi Tanaka ◽  
Mitsuko Hirosawa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Sequence Diversity ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Role of DNA methylation in the tissue-specific expression of the CYP17A1 gene for steroidogenesis in rodents

2009 ◽  
Vol 202 (1) ◽  
pp. 99-109 ◽  
Author(s):  
Elika Missaghian ◽  
Petra Kempná ◽  
Bernhard Dick ◽  
Andrea Hirsch ◽  
Rasoul Alikhani-Koupaei ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Histone Deacetylase Inhibitors ◽  
Cpg Island ◽  
Specific Expression ◽  
Tissue Specific ◽  
Adrenal Cells ◽  
Tissue Specific Expression ◽  
As Species ◽  
Species Specific

The CYP17A1 gene is the qualitative regulator of steroidogenesis. Depending on the presence or absence of CYP17 activities mineralocorticoids, glucocorticoids or adrenal androgens are produced. The expression of the CYP17A1 gene is tissue as well as species-specific. In contrast to humans, adrenals of rodents do not express the CYP17A1 gene and have therefore no P450c17 enzyme for cortisol production, but produce corticosterone. DNA methylation is involved in the tissue-specific silencing of the CYP17A1 gene in human placental JEG-3 cells. We investigated the role of DNA methylation for the tissue-specific expression of the CYP17A1 gene in rodents. Rats treated with the methyltransferase inhibitor 5-aza-deoxycytidine excreted the cortisol metabolite tetrahydrocortisol in their urine suggesting that treatment induced CYP17 expression and 17α-hydroxylase activity through demethylation. Accordingly, bisulfite modification experiments identified a methylated CpG island in the CYP17 promoter in DNA extracted from rat adrenals but not from testes. Both methyltransferase and histone deacetylase inhibitors induced the expression of the CYP17A1 gene in mouse adrenocortical Y1 cells which normally do not express CYP17, indicating that the expression of the mouse CYP17A1 gene is epigenetically controlled. The role of DNA methylation for CYP17 expression was further underlined by the finding that a reporter construct driven by the mouse −1041 bp CYP17 promoter was active in Y1 cells, thus excluding the lack of essential transcription factors for CYP17 expression in these adrenal cells.

scholarly journals Hypermethylation of human DNA: Fine-tuning transcription associated with development

2017 ◽  
Author(s):  
Carl Baribault ◽  
Kenneth C. Ehrlich ◽  
V. K. Chaithanya Ponnaluri ◽  
Sriharsa Pradhan ◽  
Michelle Lacey ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Regulatory Elements ◽  
Fine Tuning ◽  
Ctcf Binding ◽  
Specific Gene ◽  
Dna Hypermethylation ◽  
Specific Expression ◽  
Lncrna Gene ◽  
Tissue Specific ◽  
Tissue Specific Expression

AbstractTissue-specific gene transcription can be affected by DNA methylation in ways that are difficult to discern from studies focused on genome-wide analyses of differentially methylated regions (DMRs). We studied 95 genes in detail using available epigenetic and transcription databases to detect and elucidate less obvious associations between development-linked hypermethylated DMRs in myoblasts (Mb) and cell-and tissue-specific expression. Many of these genes encode developmental transcription factors and display DNA hypermethylation also in skeletal muscle (SkM) and a few heterologous samples (e.g., aorta, mammary epithelial cells, or brain) among the 38 types of human cell cultures or tissues examined. Most of the DMRs overlapped transcription regulatory elements, including canonical, alternative, or cryptic promoters; enhancers; CTCF binding sites; and long-noncoding RNA (lncRNA) gene regions. Among the prominent relationships between DMRs and expression was promoter-region hypermethylation accompanying repression in Mb but not in many other repressed samples (26 genes). Another surprising relationship was down-modulated (but not silenced) expression in Mb associated with DNA hypermethylation at cryptic enhancers in Mb although such methylation was absent in both non-expressing samples and highly expressing samples (24 genes). The tissue-specificity of DNA hypermethylation can be explained for many of the genes by their roles in prenatal development or by the tissue-specific expression of neighboring genes. Besides elucidating developmental epigenetics, our study provides insights into the roles of abnormal DNA methylation in disease, e.g., cancer, Duchenne muscular dystrophy, and congenital heart malformations.

Sign in / Sign up

Export Citation Format

Share Document