scholarly journals Effect of High Dietary Carbohydrate on the Growth Performance and Physiological Responses of Juvenile Wuchang Bream, <italic>Megalobrama amblycephala</italic>

2013 ◽  
Vol 26 (11) ◽  
pp. 1598-1608 ◽  
Author(s):  
C. P. Zhou ◽  
X. P. Ge ◽  
B. Liu ◽  
J. Xie ◽  
L. H. Miao
Keyword(s):  
Growth Performance ◽  
Physiological Responses ◽  
Megalobrama Amblycephala ◽  
Dietary Carbohydrate ◽  
Wuchang Bream

Interactions between dietary carbohydrate and thiamine: Implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala

2021 ◽  
pp. 1-38
Author(s):  
Chao Xu ◽  
Yuan-You Li ◽  
Paul B. Brown ◽  
Wen-Bin Liu ◽  
Liu-Ling Gao ◽  
...  
Keyword(s):  
Protein Expression ◽  
Growth Performance ◽  
Mitochondrial Biogenesis ◽  
Complex I ◽  
Megalobrama Amblycephala ◽  
Dietary Carbohydrate ◽  
High Carbohydrate ◽  
Carbohydrate Levels ◽  
And Function ◽  
Cox 1

Abstract A 12-week experiment was conducted to evaluate the influences of thiamine on growth performance and intestinal mitochondrial biogenesis and function of M. amblycephala fed a high-carbohydrate (HC) diet. Fish (24.73 ± 0.45 g) were randomly assigned one of four diets; two carbohydrate levels (30 and 45%) and two thiamine levels (0 and 1.5 mg kg-1). HC diets significantly decreased DGC, GRMBW, FIMBW, intestinal activities of amylase, lipase, Na+, K+-ATPase, CK, complex I, III and IV, intestinal microvilli length, number of mitochondrial per field, ΔΨm, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, TFAM, Opa-1, ND-1 and COX-1 and 2, while the opposite was true for ATP, AMP and ROS, and the transcriptions of Drp-1, Fis-1 and Mff. Dietary thiamine concentrations significantly increased DGC, GRMBW, intestinal activities of amylase, Na+, K+-ATPase, CK, complex I and IV, intestinal microvilli length, number of mitochondrial per field, ΔΨm, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, ND-1, COX-1 and 2, SGLT-1 and GLUT-2. Furthermore, a significant interaction between dietary carbohydrate and thiamine was observed in DGC, GRMBW, intestinal activities of amylase, CK, complex I and IV, ΔΨm, the AMP/ATP ratio, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, COX-1 and 2, SGLT-1 and GLUT-2. Overall, thiamine supplementation improved growth performance, and intestinal mitochondrial biogenesis and function of M. amblycephala fed HC diets.

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