scholarly journals Effect of Total Mixed Ration with Fermented Feed on Ruminal In vitro Fermentation, Growth Performance and Blood Characteristics of Hanwoo Steers

2011 ◽  
Vol 25 (2) ◽  
pp. 213-223 ◽  
Author(s):  
S. H. Kim ◽  
M. J. Alam ◽  
M. J. Gu ◽  
K. W. Park ◽  
C. O. Jeon ◽  
...  
Keyword(s):  
Growth Performance ◽  
In Vitro Fermentation ◽  
Total Mixed Ration ◽  
Fermented Feed ◽  
Blood Characteristics

scholarly journals Effect of exogenous glucoamylase inclusion on in vitro fermentation and growth performance of feedlot steers fed a dry-rolled corn-based diet

2021 ◽  
Author(s):  
A M Pittaluga ◽  
S Yu ◽  
W Li ◽  
J C McCann
Keyword(s):  
Growth Performance ◽  
Batch Culture ◽  
Dry Matter ◽  
Carcass Characteristics ◽  
Feed Conversion ◽  
Muscle Area ◽  
In Vitro Fermentation ◽  
Molar Proportion ◽  
Feedlot Steers

Abstract The objective was to evaluate the effect of adding an exogenous glucoamylase (GA) enzyme from the fungus Trichoderma reesei on in vitro fermentation, growth performance, and carcass characteristics of feedlot steers fed a dry-rolled corn-based diet. Experiment 1 evaluated 3 levels of added enzyme (0, 0.24, and 0.72 GA enzyme units) and 2 corn particle sizes (CPS; 2 and 4 mm) in a factorial arrangement using a 7 h in vitro batch culture fermentation. Addition of GA increased (P < 0.01) in vitro dry matter disappearance by 13% and decreased final pH (P < 0.01). Molar proportion of propionate increased with GA inclusion (P < 0.01). A smaller CPS increased (P < 0.01) in vitro dry matter disappearance and total volatile fatty acid and decreased final pH (P < 0.01). A smaller CPS also decreased (P < 0.01) the molar proportion of acetate and increased (P < 0.01) the molar proportion of butyrate. In Experiment 2, Angus × Simmental steers (n = 105; initial BW = 329 ± 38 kg) were used to evaluate the inclusion of an exogenous GA on growth performance and carcass characteristics. Steers were fed a basal diet consisting of 60% dry-rolled corn, 17.5% modified distillers grains with solubles, 12.5% corn silage and 10% dry supplement on a dry matter basis for 136 d. Steers were blocked by weight and allotted to pens. Pens were randomly assigned to one of 3 treatments (5 pens/treatment): diet with no GA (CON), low inclusion of GA (122 enzyme units/kg DM; LGA), or high inclusion of GA (183 enzyme units/kg DM; HGA). Inclusion of GA did not affect (P ≥ 0.23) final BW, DMI, or ADG for the 136-d feeding period. Feed conversion was affected (P = 0.02) by treatment with steers fed HGA having ~8% greater G:F compared with LGA and CON. Treatment did not affect (P = 0.32) fecal starch. Inclusion of GA did not affect (P ≥ 0.19) carcass traits including HCW, 12 th rib fat thickness, yield grade, longissimus muscle area, or marbling score. Overall, results suggest inclusion of exogenous GA enzyme increased in vitro dry matter disappearance in batch culture and improved feed conversion in steers fed 183 enzyme units/kg DM during the finishing phase.

scholarly journals The Effects of Aqueous Fibrolytic Enzyme Products Supplementation on In vitro Fermentation and Growth Performance of Hanwoo Steers

2009 ◽  
Vol 51 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Kil Kook ◽  
Wan-Tae Chung ◽  
Hong-Seok Park ◽  
Ki-Yeoung Jang ◽  
Ji-Wung Lee ◽  
...  
Keyword(s):  
Growth Performance ◽  
In Vitro Fermentation

Effect of water quality on in vitro fermentation of sorghum and barley for poultry diets

2007 ◽  
Vol 2007 ◽  
pp. 243-243
Author(s):  
A.T. Niba ◽  
J.D. Beal ◽  
A.C. Kudi ◽  
P.H. Brooks
Keyword(s):  
Water Quality ◽  
Lactic Acid ◽  
Acid Concentration ◽  
Lactic Acid Concentration ◽  
In Vitro Fermentation ◽  
Effect Of Water ◽  
Fermented Feed ◽  
Fermentation Pattern ◽  
Poultry Diets

Low pH and high lactic acid concentration of fermented feed has been reported to be responsible for the antimicrobial activity of fermented feeds (Brooks et al., 2001). For example, to prevent the growth of Salmonella spp. in liquid feeds, a threshold lactic acid concentration of 75mM is required (Beal et al., 2002). Therefore, factors that are likely to affect the production of lactic acid during fermentation will have important implications for the ability of such feeds to withstand colonisation by pathogens. The objective of the present study was to investigate the effect of water quality on the fermentation pattern of sorghum and barley.

scholarly journals Preservation of Human Gut Microbiota Inoculums for In Vitro Fermentations Studies

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Nelson Mota de Carvalho ◽  
Diana Luazi Oliveira ◽  
Mayra Anton Dib Saleh ◽  
Manuela Pintado ◽  
Ana Raquel Madureira
Keyword(s):  
Gut Microbiota ◽  
Metabolic Profile ◽  
Bacterial Count ◽  
Glycerol Solution ◽  
Metabolic Profiles ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro Fermentation ◽  
Fecal Samples

The use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at −20 °C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at −20 °C for at least 3 months, without interfering with the normal course of colonic fermentation.

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