scholarly journals Effect of a Mixture of Conjugated Linoleic Acid (CLA) Isomers on T Cell Subpopulation and Responsiveness to Mitogen in Splenocytes of Male Broiler Chicks

2007 ◽  
Vol 20 (6) ◽  
pp. 954-961 ◽  
Author(s):  
Kazuaki Takahashi ◽  
Kenji Kawamata ◽  
Yukio Akiba
Keyword(s):  
Linoleic Acid ◽  
T Cell ◽  
Conjugated Linoleic Acid ◽  
Cell Subpopulation ◽  
Broiler Chicks ◽  
Cla Isomers

Conjugated linoleic acid (CLA) isomers in human adipose tissue

1997 ◽  
Vol 205 (6) ◽  
pp. 415-418 ◽  
Author(s):  
J. Fritsche ◽  
Magdi M. Mossoba ◽  
Martin P. Yurawecz ◽  
John A. G. Roach ◽  
Najibullah Sehat ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Human Adipose Tissue ◽  
Cla Isomers

scholarly journals Conjugated linoleic acid enhanced the immune function in broiler chicks

2005 ◽  
Vol 94 (5) ◽  
pp. 746-752 ◽  
Author(s):  
Haijun Zhang ◽  
Yuming Guo ◽  
Jianmin Yuan
Keyword(s):  
Linoleic Acid ◽  
Growth Performance ◽  
Conjugated Linoleic Acid ◽  
Antibody Production ◽  
Peripheral Blood ◽  
Lysozyme Activity ◽  
Control Group ◽  
Prostaglandin E ◽  
Broiler Chicks ◽  
Dietary Treatments

This study was undertaken to investigate the growth performance and immune responses of broiler chicks fed diets supplemented with conjugated linoleic acid (CLA). Two hundred and forty day-old Arbor Acre male broiler chicks were randomly allotted into four dietary treatments with different inclusion levels of CLA (0, 2·5, 5·0 or 10·0g pure CLA/kg) for 6 weeks. Growth performance, lysozyme activity, peripheral blood mononuclear cell (PBMC) proliferation, prostaglandin E2 (PGE2) synthesis and antibody production were investigated. There were no significant differences in growth performance among treatments (P>0·05). Chicks fed 10·0g CLA/kg diet produced 40% and 49% more lysozyme activity in serum and spleen than the control group at 21d of age (P<0·05). Dietary CLA enhanced the PBMC proliferation in response to concanavalin A at the age of 21 and 42d (P<0·05). Systemic and peripheral blood lymphocytic synthesis of PGE2 in chicks fed 10·0g CLA/kg diet was significantly decreased by 57% and 42% compared to chicks fed control diet (P<0·05). Antibody production to sheep red blood cell and bovine serum albumin were elevated in either 2·5 or 10·0g CLA/kg dietary treatments (P<0·05). The results indicated dietary CLA could enhance the immune response in broiler chicks, but did not alter the growth performance.

scholarly journals  A new internal standard for HPLC assay of conjugated linoleic acid in animal tissues and milk

2011 ◽  
Vol 56 (No. 1) ◽  
pp. 23-29 ◽  
Author(s):  
M. Czauderna ◽  
J. Kowalczyk ◽  
M. Marounek ◽  
J.P. Michalski ◽  
A.J. Rozbicka-Wieczorek
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Biological Samples ◽  
Mobile Phase ◽  
Sorbic Acid ◽  
Internal Standard ◽  
Silver Ion ◽  
Animal Origin ◽  
Cla Isomers

A new method for the quantification of underivatized conjugated linoleic acid (CLA) isomers and CLA-metabolites by silver ion liquid chromatography (Ag<sup>+</sup>-HPLC) with photodiode array detection (DAD) is described. Conjugated fatty acids (CFA) and sorbic acid as the internal standard (IS) were separated on two 5 &mu;m Chrompac ChromSpher Lipids columns (250 &times; 4.6 mm). Biological samples were hydrolyzed with 1M KOH in methanol and 2M KOH in water at room temperature for 12 h. Hydrolyzates were acidified and the free fatty acids were extracted with dichloromethane. The organic solvent was removed and then the residue was re-dissolved in hexane and centrifuged. The supernatant was injected onto the columns. The mobile phase of 1.6% acetic acid and 0.0125% acetonitrile in hexane was chosen as the optimum mobile phase for fractionation of IS, CLA isomers and CLA-metabolites in all assayed biological samples. The use of two silver ion-exchange columns with direct UV detection (Ag<sup>+</sup>-HPLC-DAD) offers satisfactory precision of the IS quantification and low limits of detection of IS and CLA isomers (0.60 and 0.21&ndash;0.35 ng, respectively). The presented simple Ag<sup>+</sup>-HPLC-DAD method with sorbic acid as the IS can be used for direct determination of underivatized CLA isomers in specimens of animal origin. &nbsp;

Comparison of Methylation Methods for the Quantitation of Conjugated Linoleic Acid Isomers

1993 ◽  
Vol 76 (3) ◽  
pp. 644-649 ◽  
Author(s):  
Nalur Chandrasekaran Shantha ◽  
Eric Andrew Decker ◽  
Bernhard Hennig
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Sodium Methoxide ◽  
Boron Trifluoride ◽  
Acetyl Chloride ◽  
Octadecadienoic Acid ◽  
Methyl Esters ◽  
Low Concentrations ◽  
Cla Isomers ◽  
In Cis

Abstract Four methylation methods were evaluated for use in the gas chromatographic (GC) quantitation of conjugated linoleic acid (CLA) isomers, which are potential anticarcinogen. The methods were (1) sodium methoxide in methanol (NaOMe-MeOH), (2) American Oil Chemists' Society (AOCS) procedure Ce 2-66, which involves methanolic sodium hydroxide followed by boron trifluoride in methanol, (3) tetramethylguanidine in methanol (TMG-MeOH), and (4) direct transesterification with methanolbenzene- acetyl chloride (DAC). Purified methyl esters of isomerized linoleic acid containing 86% CLA isomers were methylated and analyzed by GC. The AOCS and DAC methods resulted in 3 and 50% losses in cis-9,trans-11-octadecadienoic acid (9c, 111 CLA isomer) and trans-10,cis-12 octadecadienoic acid (10t, 12c CLA isomers), respectively. Compared with the control, the AOCS and DAC methods increased the yield of the trans,trans CLA isomers (trans-9,trans-11- and trans-10, trans-12-octadecadienoic acid) by 1.07-fold and a 10-fold, respectively. A non-CLA artifact that eluted close to CLA peaks was formed during methylation by the AOCS and DAC methods. Thus, the DAC and AOCS methods are not suitable for quantitation of CLA isomers. The NaOMe-MeOH and TMG-MeOH methods, however, are suitable for quantitation of CLA isomers in fats containing low concentrations of free fatty acids.

scholarly journals Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster

1999 ◽  
Vol 82 (4) ◽  
pp. 309-317 ◽  
Author(s):  
Emile A. M. de Deckere ◽  
Johan M. M. van Amelsvoort ◽  
Gerald P. McNeill ◽  
Penny Jones
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Hdl Cholesterol ◽  
Liver Weight ◽  
Peroxisome Proliferation ◽  
Lipid Levels ◽  
Fat Pad ◽  
Active Isomer ◽  
Carnitine Acetyl Transferase ◽  
Cla Isomers

Effects of the conjugated linoleic acid (CLA) isomers cis-9, trans-11 (c9, t11 CLA) and trans-10, cis-12 (t10, c12 CLA) on lipid metabolism and markers of peroxisome proliferation were investigated in hamsters fed on purified diets containing 30 % energy as fat and 0·1 g cholesterol/kg for 8 weeks. Four groups (n 32 each) received diets without CLA (control), with a mixture of equal amounts of c9, t11 and t10, c12 CLA (CLA mix), with c9, t11 CLA, and with t10, c12 CLA. The total amount of CLA isomers was 1·5 % energy or 6·6 g/kg diet. CLA was incorporated into glycerides and exchanged for linoleic acid in the diet. Compared with the control, the CLA mix and t10, c12 CLA decreased fasting values of LDL- (21 and 18 % respectively) and HDL-cholesterol (8 and 11 %), increased VLDL-triacylglycerol (80 and 61 %), and decreased epididymal fat pad weights (9 and 16 %), whereas c9, t11 CLA had no significant effects. All CLA preparations increased liver weight, but not liver lipids. However, the increase in liver weight was much less in the c9, t11 CLA group (8 %) than in the other two groups (25 %) and might have been caused by the small amount of t10, c12 CLA present in the c9, t11 CLA preparation. Liver histology revealed that increased weight was due to hypertrophy. Markers of peroxisome proliferation, such as cyanide-insensitive palmitoyl CoA oxidase (EC 1.3.3.6) and carnitine acetyl transferase (EC 2.3.1.7) activities, were not increased by CLA. Both c9, t11 CLA and t10, c12 CLA were incorporated into phospholipids and triacylglycerols, but t10, c12 CLA only about half as much as c9, t11 CLA. In addition, linoleic acid and linolenic acid concentrations were lower in lipids of the t10, c12 CLA group compared with the c9, t11 CLA group. These data suggest that t10, c12 CLA stimulated the oxidation of all C18 polyunsaturated fatty acids. The results indicate that the t10, c12 CLA isomer, and not the so-called natural CLA isomer (c9, t11), is the active isomer affecting lipid levels in hamsters.

The effect of different conjugated linoleic acid (CLA) isomers on fat distribution in women

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Marianne Raff ◽  
Tine Tholstrup ◽  
Søren Toubro ◽  
Pia Lund ◽  
Ellen Marie Straarup
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Fat Distribution ◽  
Cla Isomers
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