scholarly journals Insertional Variations of Two Porcine Endogenous Retroviruses (PERVs) in Korean Native Pigs and Asian Wild Boars

2007 ◽  
Vol 20 (4) ◽  
pp. 461-465 ◽  
Author(s):  
K. C. Jung ◽  
S. L. Yu ◽  
T. H. Kim ◽  
J. T. Jeon ◽  
C. Rogel-Gaillard ◽  
...  
Keyword(s):  
Endogenous Retroviruses ◽  
Wild Boars ◽  
Porcine Endogenous Retroviruses ◽  
Korean Native Pigs ◽  
Native Pigs

scholarly journals Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars

Viruses ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Luise Krüger ◽  
Milena Stillfried ◽  
Carolin Prinz ◽  
Vanessa Schröder ◽  
Lena Katharina Neubert ◽  
...  
Keyword(s):  
Wild Boar ◽  
Copy Number ◽  
Digital Pcr ◽  
Endogenous Retroviruses ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Copy Numbers ◽  
Cellular Genes ◽  
Porcine Endogenous Retroviruses ◽  
Different Populations

Porcine endogenous retroviruses (PERVs) are integrated in the genome of pigs and are transmitted like cellular genes from parents to the offspring. Whereas PERV-A and PERV-B are present in all pigs, PERV-C was found to be in many, but not all pigs. When PERV-C is present, recombination with PERV-A may happen and the PERV-A/C recombinants are characterized by a high replication rate. Until now, nothing has been known about the copy number of PERVs in wild boars and little is known about the prevalence of the phylogenetically youngest PERV-C in ancient wild boars. Here we investigated for the first time the copy number of PERVs in different populations of wild boars in and around Berlin using droplet digital PCR. Copy numbers between 3 and 69 per genome have been measured. A lower number but a higher variability was found compared to domestic pigs, including minipigs reported earlier (Fiebig et al., Xenotransplantation, 2018). The wild boar populations differed genetically and had been isolated during the existence of the Berlin wall. Despite this, the variations in copy number were larger in a single population compared to the differences between the populations. PERV-C was found in all 92 analyzed animals. Differences in the copy number of PERV in different organs of a single wild boar indicate that PERVs are also active in wild boars, replicating and infecting new cells as has been shown in domestic pigs.

scholarly journals Characteristic features of porcine endogenous retroviruses in Vietnamese native pigs

2020 ◽  
Vol 91 (1) ◽  
Author(s):  
Shinya Ishihara ◽  
Thanh Q. Dang‐Nguyen ◽  
Kazuhiro Kikuchi ◽  
Aisaku Arakawa ◽  
Satoshi Mikawa ◽  
...  
Keyword(s):  
Endogenous Retroviruses ◽  
Porcine Endogenous Retroviruses ◽  
Characteristic Features ◽  
Native Pigs

scholarly journals Determination of the copy numbers of type A porcine endogenous retroviruses in domestic pigs and wild boars

2016 ◽  
Vol 20 (6) ◽  
pp. 756-761 ◽  
Author(s):  
R. B. Aitnazarov ◽  
N. S. Yudin ◽  
R. S. Kiril’chuk ◽  
N. N. Kochnev ◽  
S. P. Knyazev ◽  
...  
Keyword(s):  
Type A ◽  
Endogenous Retroviruses ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Copy Numbers ◽  
Porcine Endogenous Retroviruses

Unexpected low expression of porcine endogenous retroviruses (PERVs) in porcine expanded potential stem cells (EPSCs)

2021 ◽  
pp. 198295
Author(s):  
Luise Krüger ◽  
Monika Nowak-Imialek ◽  
Yannick Kristiansen ◽  
Doris Herrmann ◽  
Björn Petersen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endogenous Retroviruses ◽  
Porcine Endogenous Retroviruses ◽  
Low Expression

scholarly journals Absence of Replication-Competent Human-Tropic Porcine Endogenous Retroviruses in the Germ Line DNA of Inbred Miniature Swine

2004 ◽  
Vol 78 (5) ◽  
pp. 2502-2509 ◽  
Author(s):  
Linda Scobie ◽  
Samantha Taylor ◽  
James C. Wood ◽  
Kristen M. Suling ◽  
Gary Quinn ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Germ Line ◽  
Human Cells ◽  
Endogenous Retroviruses ◽  
Open Reading Frames ◽  
Miniature Swine ◽  
Dna Libraries ◽  
Porcine Endogenous Retroviruses ◽  
Perv Transmission

ABSTRACT The potential transmission of porcine endogenous retroviruses (PERVs) has raised concern in the development of porcine xenotransplantation products. Our previous studies have resulted in the identification of animals within a research herd of inbred miniature swine that lack the capacity to transmit PERV to human cells in vitro. In contrast, other animals were capable of PERV transmission. The PERVs that were transmitted to human cells are recombinants between PERV-A and PERV-C in the post-VRA region of the envelope (B. A. Oldmixon, J. C. Wood, T. A. Ericsson, C. A. Wilson, M. E. White-Scharf, G. Andersson, J. L. Greenstein, H. J. Schuurman, and C. Patience, J. Virol. 76:3045-3048, 2002); these viruses we term PERV-A/C. This observation prompted us to determine whether these human-tropic replication-competent (HTRC) PERV-A/C recombinants were present in the genomic DNA of these miniature swine. Genomic DNA libraries were generated from one miniature swine that transmitted HTRC PERV as well as from one miniature swine that did not transmit HTRC PERV. HTRC PERV-A/C proviruses were not identified in the germ line DNAs of these pigs by using genomic mapping. Similarly, although PERV-A loci were identified in both libraries that possessed long env open reading frames, the Env proteins encoded by these loci were nonfunctional according to pseudotype assays. In the absence of a germ line source for HTRC PERV, further studies are warranted to assess the mechanisms by which HTRC PERV can be generated. Once identified, it may prove possible to generate animals with further reduced potential to produce HTRC PERV.

scholarly journals Sequence-based characterization of the eight SLA loci in Korean native pigs

2008 ◽  
Vol 35 (4-5) ◽  
pp. 333-334 ◽  
Author(s):  
Y. J. Lee ◽  
K. H. Cho ◽  
M. J. Kim ◽  
D. M. Smith ◽  
C. S. Ho ◽  
...  
Keyword(s):  
Korean Native Pigs ◽  
Native Pigs

Establishment of a resource population of SLA haplotype-defined Korean native pigs

2010 ◽  
Vol 29 (5) ◽  
pp. 493-499 ◽  
Author(s):  
Han-Ok Cho ◽  
Chak-Sum Ho ◽  
Yu-Joo Lee ◽  
In-Cheol Cho ◽  
Sung-Soo Lee ◽  
...  
Keyword(s):  
Resource Population ◽  
Korean Native Pigs ◽  
Native Pigs
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