scholarly journals Sexing Goat Embryos by PCR Amplification of X- and Y- chromosome Specific Sequence of the Amelogenin Gene

2007 ◽  
Vol 20 (11) ◽  
pp. 1689-1693 ◽  
Author(s):  
A-qin Chen ◽  
Zi-rong Xu ◽  
Song-dong Yu
Keyword(s):  
Y Chromosome ◽  
Pcr Amplification ◽  
Specific Sequence ◽  
Amelogenin Gene

Trypanosomatidae:PhytomonasDetection in Plants and Phytophagous Insects by PCR Amplification of a Genus-Specific Sequence of the Spliced Leader Gene

1999 ◽  
Vol 91 (3) ◽  
pp. 268-279 ◽  
Author(s):  
Myrna G. Serrano ◽  
Luiz R. Nunes ◽  
Marta Campaner ◽  
Gregory A. Buck ◽  
Erney P. Camargo ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Phytophagous Insects ◽  
Specific Sequence ◽  
Spliced Leader

214 PRE-IMPLANTATION EMBRYO SEX DETERMINATION BY AMPLIFICATION OF AMELOGENIN GENE IN WATER BUFFALOES (BUBALUS BUBALIS)

2016 ◽  
Vol 28 (2) ◽  
pp. 238
Author(s):  
J.-H. Shang ◽  
C.-Y. Yang ◽  
H.-Y. Zheng ◽  
J. Qin ◽  
Y.-P. Gu ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Natural Science ◽  
Water Buffalo ◽  
Tooth Enamel ◽  
Mammalian Species ◽  
Early Embryo ◽  
Specific Pattern ◽  
Specific Sequence ◽  
Embryo Sex

Sex-specific sequence variability of the amelogenin (AMEL) gene has been observed in a variety of mammalian species. AMEL is a multi-copy gene expressed in abundance in the development of mammalian tooth enamel matrix. Its homologous genes are located on the X- or Y-chromosome, respectively. In our study, a pair of primers was designed to allow amplification of a single fragment of 312 bp from buffalo cows. Because the gene is slightly differently encoded on the Y-chromosome, the same primers were expected to produce 2 fragments of 312 and 249 bp from bulls. The primers were successfully applied to single, in vitro-produced Day-6 to Day-8 water buffalo blastocysts by direct lysis of the whole blastocysts, followed by PCR. The amplification on presumed female and male embryos consistently displayed the same sex-specific pattern. Of 100 blastocysts, 43 were determined to be females, 51 males, and 6 unknown (samples missed). We concluded that PCR amplification of the AMEL gene can be used for early embryo sex identification of water buffalo. This work was supported by the National Natural Science Foundation of China under Grant No. 31160456 and the Guangxi Natural Science Foundation of China under Grants No. 2013GXNSFAA019075 and 2014GXNSFAA118116.

RAPD analysis of jasmine rice-specific genomic structure

Genome ◽  
2006 ◽  
Vol 49 (6) ◽  
pp. 716-719 ◽  
Author(s):  
Y J Wu ◽  
Y Chen ◽  
J Wang ◽  
C X Zhu ◽  
B L Xu
Keyword(s):  
Oryza Sativa L ◽  
Genomic Structure ◽  
Pcr Amplification ◽  
Disease Resistance Gene ◽  
Japonica Rice ◽  
Rice Seed ◽  
Specific Sequence ◽  
First Intron ◽  
Jasmine Rice ◽  
True Relation

Total genomic DNA was extracted from 29 samples of rice seed, including jasmine rice Oryza sativa L. subsp indica 'KDML105', 'KDML105'-derived varieties, nonaromatic Thailand rice, and japonica rice. Polymorphism in RAPD profiles was analyzed to explore the genomic structure specific to jasmine rice. The degree of band sharing was used to evaluate genetic distance between varieties and to construct a phylogenetic tree. RD15, CNTLR85033, and CNT87040 were found to be closest to 'KDML105', which was consistent with the true relation among them. Four RAPD fragments that cooperatively distinguished jasmine rice from others were cloned and sequenced. PCR amplification using pairs of primers designed specifically further confirmed the credibility of the RAPD result. Comparison through Genbank revealed that a 454 bp RAPD band was similar to the first intron of a putative Cf2/Cf5 disease resistance gene and a 1107 bp RAPD band similar to a wall-associated kinase (wak) gene sequence.Key words: Jasmine rice, RAPD, specific sequence.

Use of Ethnicity-Specific Sequence Tag Site Markers for Y Chromosome Microdeletion Studies

2011 ◽  
Vol 15 (6) ◽  
pp. 451-459 ◽  
Author(s):  
Kabir Sachdeva ◽  
Renu Saxena ◽  
Abha Majumdar ◽  
Sudhir Chadda ◽  
Ishwar Chander Verma
Keyword(s):  
Y Chromosome ◽  
Specific Sequence ◽  
Y Chromosome Microdeletion ◽  
Site Markers

scholarly journals A sexing protocol for wild ruminants based on PCR amplification of amelogenin genes AMELX and AMELY (short communication)

2007 ◽  
Vol 50 (5) ◽  
pp. 442-446 ◽  
Author(s):  
G. Pajares ◽  
I. Álvarez ◽  
I. Fernández ◽  
L. Pérez-Pardal ◽  
F. Goyache ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Y Chromosome ◽  
X Chromosome ◽  
Short Communication ◽  
Pcr Amplification ◽  
Sex Identification ◽  
Protocol Design ◽  
Wild Ruminant ◽  
Wild Ruminants ◽  
Ruminant Species

Abstract. Based on the sequences of the bovine amelogenin genes, we have designed a protocol for sexing DNA samples of wild ruminants. Basically the protocol consists on the co-amplification of two specific fragments, one from Y-chromosome and one for the X chromosome, making the use of a PCR control unnecessary. It has been demonstrated to be useful for sex identification in a total of 164 samples belonging to six different wild ruminant species. We propose adding to the census procedure commonly based in faecal groups counting, the faecal sampling and application of the protocol design here, to estimate the sex ratio.

scholarly journals Chromosomal abnormalities and Y chromosome microdeletions in infertile men with azoospermia and oligozoospermia in Eastern China

2019 ◽  
Vol 48 (4) ◽  
pp. 030006051989671
Author(s):  
Jing Sha ◽  
Guiping Huang ◽  
Bei Zhang ◽  
Xia Wang ◽  
Zaochun Xu ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Assisted Reproduction ◽  
Sex Chromosome ◽  
Chromosomal Abnormalities ◽  
Eastern China ◽  
Peripheral Blood Lymphocytes ◽  
Specific Sequence ◽  
Sequence Tagged Sites ◽  
Infertile Men ◽  
Y Chromosome Microdeletions

Objective The objective was to investigate the frequency and type of chromosomal abnormalities and Y chromosome microdeletions in infertile men with azoospermia and oligozoospermia to ensure appropriate genetic counseling before assisted reproduction in Eastern China. Methods A total of 201 infertile men (148 with azoospermia and 53 with oligozoospermia) were enrolled. Real-time PCR using six Y-specific sequence-tagged sites of the azoospermia factor (AZF) region was performed to screen for microdeletions. Karyotype analyses were performed on peripheral blood lymphocytes with standard G-banding. Results Out of 201 infertile patients, 22 (10.95%) had Y microdeletions [17/148 (11.49%) men with azoospermia and 5/53 (9.43%) men with oligozoospermia]. The most frequent microdeletions were in the AZFc region, followed by the AZFa+b + c, AZFb+c, AZFa, and AZFb regions. Chromosomal abnormalities were detected in 18.91% (38/201) of patients, 34 of which were sex chromosome abnormalities (16.92%) and 4 of which were autosomal abnormalities (1.99%). Chromosomal abnormalities were more prevalent in men with azoospermia (22.97%) than in those with oligozoospermia (7.55%). Conclusions We detected a high incidence of chromosomal abnormalities and Y chromosomal microdeletions in infertile Chinese men with azoospermia and oligozoospermia. These findings suggest the need for genetic testing before the use of assisted reproduction techniques.

scholarly journals Phenotypic and molecular characterization of HA-MRSA in Taif hospitals, Saudi Arabia

2015 ◽  
Vol 9 (03) ◽  
pp. 298-303 ◽  
Author(s):  
Emad M Eed ◽  
Mabrouk M Ghonaim ◽  
Yousry M Hussein ◽  
Taiser M Saber ◽  
Amany S Khalifa
Keyword(s):  
Saudi Arabia ◽  
Gene Polymorphism ◽  
Pcr Amplification ◽  
Clinical Samples ◽  
Molecular Characteristics ◽  
Polymorphism Analysis ◽  
Specific Sequence ◽  
Mrsa Strains ◽  
Hospital Acquired ◽  
Sccmec Types

Introduction: Methicillin-resistant S. aureus (MRSA) is one of the most important organisms causing hospital-acquired infections worldwide. Molecular analysis of MRSA strains from Taif, Saudi Arabia, had not been previously done. Phenotypic and molecular characteristics of MRSA isolated from Taif hospitals were investigated. Methodology: This study involved S. aureus strains isolated from different clinical samples from Taif hospitals. MRSA strains were identified and antimicrobial susceptibility profiles were determined. Multiplex polymerase chain reaction (PCR) was used to identify S. aureus-specific sequence, mecA genes, and type of staphylococcal cassette chromosome mec (SCCmec). MRSA strains were typed using coagulase gene polymorphism. Results: In total, 390 strains of S. aureus were isolated, and 58 MRSA strains – 40 hospital-acquired  MRSA (HA-MRSA) and 18 community-acquired MRSA (CA-MRSA) – were detected. HA-MRSA strains included three SCCmec types, while CA-MRSA strains included two SCCmec types. PCR amplification and restriction of the coagulase gene of the 58 MRSA isolates showed nine different patterns, while three strains were non-typable. HA-MRSA strains showed seven distinct restriction fragment length polymorphism (RFLP) patterns; the most frequent was pattern 2 (15 isolates), followed by patterns 1 and 4 (5 isolates each). CA-MRSA showed five RFLP patterns; the most frequent was pattern 3 (7 isolates) followed by pattern 8 (6 isolates). Conclusions: HA-MRSA strains were more common than CA-MRSA strains. SCCmec typing and coagulase gene polymorphism analysis may be useful methods for studying clonal relatedness of isolates and for discriminating between HA-MRSA and CA-MRSA.

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