scholarly journals Phenotypic and molecular characterization of HA-MRSA in Taif hospitals, Saudi Arabia

2015 ◽  
Vol 9 (03) ◽  
pp. 298-303 ◽  
Author(s):  
Emad M Eed ◽  
Mabrouk M Ghonaim ◽  
Yousry M Hussein ◽  
Taiser M Saber ◽  
Amany S Khalifa
Keyword(s):  
Saudi Arabia ◽  
Gene Polymorphism ◽  
Pcr Amplification ◽  
Clinical Samples ◽  
Molecular Characteristics ◽  
Polymorphism Analysis ◽  
Specific Sequence ◽  
Mrsa Strains ◽  
Hospital Acquired ◽  
Sccmec Types

Introduction: Methicillin-resistant S. aureus (MRSA) is one of the most important organisms causing hospital-acquired infections worldwide. Molecular analysis of MRSA strains from Taif, Saudi Arabia, had not been previously done. Phenotypic and molecular characteristics of MRSA isolated from Taif hospitals were investigated. Methodology: This study involved S. aureus strains isolated from different clinical samples from Taif hospitals. MRSA strains were identified and antimicrobial susceptibility profiles were determined. Multiplex polymerase chain reaction (PCR) was used to identify S. aureus-specific sequence, mecA genes, and type of staphylococcal cassette chromosome mec (SCCmec). MRSA strains were typed using coagulase gene polymorphism. Results: In total, 390 strains of S. aureus were isolated, and 58 MRSA strains – 40 hospital-acquired  MRSA (HA-MRSA) and 18 community-acquired MRSA (CA-MRSA) – were detected. HA-MRSA strains included three SCCmec types, while CA-MRSA strains included two SCCmec types. PCR amplification and restriction of the coagulase gene of the 58 MRSA isolates showed nine different patterns, while three strains were non-typable. HA-MRSA strains showed seven distinct restriction fragment length polymorphism (RFLP) patterns; the most frequent was pattern 2 (15 isolates), followed by patterns 1 and 4 (5 isolates each). CA-MRSA showed five RFLP patterns; the most frequent was pattern 3 (7 isolates) followed by pattern 8 (6 isolates). Conclusions: HA-MRSA strains were more common than CA-MRSA strains. SCCmec typing and coagulase gene polymorphism analysis may be useful methods for studying clonal relatedness of isolates and for discriminating between HA-MRSA and CA-MRSA.

scholarly journals Prevalence Rate and Molecular Characteristics of Oestrus ovis L. (Diptera, Oestridae) in Sheep and Goats from Riyadh, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 689
Author(s):  
Dina M. Metwally ◽  
Shurug A. Albasyouni ◽  
Ibrahim A.H. Barakat ◽  
Isra M. Al-Turaiki ◽  
Amal M. Almuhanna ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Gene Sequence ◽  
Morphological Characteristics ◽  
Pcr Amplification ◽  
Infestation Rate ◽  
Molecular Characteristics ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Partial Fragment ◽  
Mtcoi Gene

Heads of sheep (n = 600) and goats (n = 800) slaughtered at Al-Aziziah Abattoir in Riyadh, Saudi Arabia, were inspected for the presence of O. ovis larvae (L). Heads were split along the longitudinal axes, and larvae (L1, L2, and L3) were gathered. The infestation rate was significantly higher in goats (44.5%; 356/800) than that in sheep (22.3%; 134/600). Out of the 151 collected larvae from sheep, 0% were L1, 1.3% were L2, and 98.7% were L3. Out of the total of 468 larvae from goats, 0% were L1, 1.2% were L2, and 98.8% were L3. The infestation rate was significantly higher in males than that in females. Myiasis-causing larvae collected from Riyadh, Saudi Arabia, were authenticated as O. ovis, according to morphological characteristics. Polymerase chain reaction (PCR) amplification of a partial fragment (600 bp) of the mitochondrial cytochrome c oxidase subunit I (mtCOI) gene further confirmed the species. Phylogenetic analysis based on the partial mtCOI gene sequence demonstrated that 23 unique sequences showed high similarity based on nucleotide pairs of O. ovis accessions retrieved from GenBank.

scholarly journals Molecular Structure and Transferability of Tn1546-Like Elements in Enterococcus faecium Isolates from Clinical, Sewage, and Surface Water Samples in Iran

2008 ◽  
Vol 74 (5) ◽  
pp. 1350-1356 ◽  
Author(s):  
M. Talebi ◽  
M. R. Pourshafie ◽  
M. Katouli ◽  
R. Möllby
Keyword(s):  
Molecular Structure ◽  
Surface Water ◽  
Molecular Analysis ◽  
Enterococcus Faecium ◽  
Pcr Amplification ◽  
Molecular Size ◽  
Municipal Sewage ◽  
Clinical Samples ◽  
Polymorphism Analysis ◽  
Hospital Sewage

ABSTRACT The molecular structure and transferability of Tn1546 in 143 vancomycin-resistant Enterococcus faecium (VREF) isolates obtained from patients (n = 49), surface water (n = 28), and urban and hospital sewage (n = 66) in Tehran, Iran, were investigated. Molecular characterization of Tn1546 elements in vanA VREF was performed using a combination of restriction fragment length polymorphism analysis and DNA sequencing of the internal PCR fragments of vanA transposons. Long-PCR amplification showed that the molecular size of Tn1546 elements varied from 10.8 to 12.8 kb. The molecular analysis of Tn1546 showed that 45 isolates (31.5%) harbored a deletion/mutation upstream from nucleotide 170. No horizontal transfer of Tn1546 was observed following filter-mating conjugation with these isolates. Nevertheless, the rates of transferability for other isolates were 10−5 to 10−6 per donor. Insertion sequences IS1216V and IS1542 were present in 103 (72%) and 138 (96.5%) of the isolates, respectively. The molecular analysis of Tn1546 elements resulted in three genomic organizations. The genomic organization lineage 1 was dominated by the isolates from clinical samples (3.4%), lineage 2 was dominated mostly by sewage isolates (24.5%), and lineage 3 contained isolates obtained from all sources (72.1%). The genetic diversity determined using pulsed-field gel electrophoresis (PFGE) revealed a single E. faecium clone, designated 44, which was common to the samples obtained from clinical specimens and hospital and municipal sewage. Furthermore, the results suggest that lineage 3 Tn1546 was highly disseminated among our enterococcal isolates in different PFGE patterns.

scholarly journals Tracking methicillin-resistant Staphylococcus aureus clones in a hospital in Southern Mexico

2020 ◽  
Vol 62 (2, Mar-Abr) ◽  
pp. 186
Author(s):  
Miguel Ángel Ortíz-Gil ◽  
Maria Elena Velazquez-Meza ◽  
Gabriela Echániz-Aviles ◽  
Javier Paul Mora-Domínguez ◽  
María Noemi Carnalla-Barajas ◽  
...  
Keyword(s):  
New York ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Hospital Environment ◽  
Molecular Characteristics ◽  
Southern Mexico ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Sequence Types ◽  
Sccmec Types

Objective. This study describes the clinical and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains that were collected in the Hospital Regional de Alta Especialidad de Veracruz (HRV). Material and Methods. A total of 107 MRSA strains from individual patients were examined in the present study. The strains examined were collected between September 2009 and September 2010. The clinical and demographic characteristics of patients were analyzed; molecular typing by pulsed-field gel electrophoresis (PFGE), staphylococcal chromosomal cassette mec (SCCmec) typing and multilocus sequence typing (MLST) were used to characterize the isolates. Results. Two PFGE patterns (NY/J and IB) were identified with 4 and 3 subtypes respectively. The isolates analyzed showed two SCCmec types (I and II) and two sequence types (ST), ST247 and ST5 related with the Iberian and New York/Japan clones respectively. Conclusion. This study establishes the presence of two very important clonal lineages of MRSA: New York/Japan and Iberian clone in hospital environment.  

MRSA/MSSA causing infections: prevalence of mecA gene

2020 ◽  
Vol 30 (Supplement_2) ◽  
Author(s):  
G Vieira ◽  
N Leal ◽  
A Rodrigues ◽  
C Chaves ◽  
F Rodrigues ◽  
...  
Keyword(s):  
Wide Spectrum ◽  
Meca Gene ◽  
Public Health Problem ◽  
Penicillin Binding Protein ◽  
Genetic Determinants ◽  
Tissue Samples ◽  
University Center ◽  
Mrsa Strains ◽  
Human Flora ◽  
Hospital Acquired

Abstract Introduction Staphylococcus aureus is part of the human flora, present in the skin and mucous membranes but can become pathogenic, causing a wide spectrum of infections that were initially treated with penicillin. However, were observed some strains with resistance to this antibiotic and, therefore was developed a new antibiotic, the methicillin. After its introduction, arose the first S. aureus with resistance to methicillin (MRSA) due to the presence of a gene known as mecA that encodes an altered penicillin binding protein (PBP2a). In Europe, it is estimated that MRSA are associated to 44% of hospital acquired infections and its mortality rate is around 20%. Objectives Prevalence of MRSA strains in different types of infection in Coimbra district. Methodology Were analysed a total of 539 isolates of S. aureus previously characterized to the antibiotic susceptibility profile in the Hospital and University Center of Coimbra. Through the minimum inhibitory concentration (MIC) of oxacillin we classified our strains into MRSA and S. aureus methicillin-sensitive (MSSA); simultaneously, the mecA gene was detected by Polymerase Chain Reaction (PCR). Results Of the 539 isolates, 49% were considered MRSA and 51% MSSA. All MRSA isolates express the mecA gene, but from the total of 276 MSSA, 191 show this gene but do not express it. MRSA isolates were mostly from respiratory tract samples (48%) and blood cultures (21%) while MSSA were isolated in skin and soft tissue samples (35%). Conclusion MRSA are considered one of the primary pathogens for the development of pneumonia and septicaemia due to its highly virulent potential and the increasing expression of genetic determinants of antimicrobial resistance. Therefore, infections caused by MRSA continue with highly representability in the clinical context and their dissemination is a public health problem.

Clinical application of cytokine-related gene polymorphism analysis using a newly developed DNA chip in critically ill patients

2009 ◽  
Vol 42 (13-14) ◽  
pp. 1387-1393 ◽  
Author(s):  
Shunsuke Otani ◽  
Shigeto Oda ◽  
Tomohito Sadahiro ◽  
Masataka Nakamura ◽  
Eizo Watanabe ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Critically Ill ◽  
Clinical Application ◽  
Critically Ill Patients ◽  
Dna Chip ◽  
Polymorphism Analysis ◽  
Related Gene

scholarly journals Genetic Characterization of Methicillin Resistant and Sensitive, Vancomycin Intermediate Staphylococcus aureus Strains Isolated from Different Iranian Hospitals

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Seyed Asghar Havaei ◽  
Amir Azimian ◽  
Hosein Fazeli ◽  
Mahmood Naderi ◽  
Kiarash Ghazvini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Analysis ◽  
Clinical Samples ◽  
Different Types ◽  
Health Care Policies ◽  
Global Health Care ◽  
Vancomycin Resistant ◽  
Epidemiological Evaluation ◽  
Mrsa Strains

Background. Global concerns have been raised due to upward trend of Vancomycin Intermediate Staphylococcus aureus (VISA) and Vancomycin Resistant Staphylococcus aureus (VRSA) reports which mean casting doubt on the absolute effectiveness of the last line of antibiotic treatment for S. aureus, vancomycin. Hence, epidemiological evaluation can improve global health care policies. Methodology. 171 Isolates of Staphylococcus aureus were collected from different types of clinical samples in selected hospitals in Isfahan, Mashhad, and Tehran, Iran. Then, they were evaluated by agar screening, disk diffusion, and MIC method to determine their resistance to vancomycin and methicillin. The isolated VISA strains were then confirmed with genetic analysis by the evaluation of mecA and vanA genes, SCCmec, agr, and spa type, and also toxin profiles. MLST was also performed. Results and Conclusion. Our data indicated that 67% of isolated S. aureus strains were resistant to methicillin. Furthermore, five isolates (2.9%) had intermediate resistance to vancomycin (VISA). In contrast to usual association of VISA with MRSA strains, we found two isolates of MSSA-VISA. Therefore, our data suggests a probable parallel growing trend of VISA towards MSSA, along with MRSA strains. However, more samples are required to confirm these primarily data. Moreover, genetic analysis of the isolated VISA strains revealed that these strains are endemic Asian clones.

Trypanosomatidae:PhytomonasDetection in Plants and Phytophagous Insects by PCR Amplification of a Genus-Specific Sequence of the Spliced Leader Gene

1999 ◽  
Vol 91 (3) ◽  
pp. 268-279 ◽  
Author(s):  
Myrna G. Serrano ◽  
Luiz R. Nunes ◽  
Marta Campaner ◽  
Gregory A. Buck ◽  
Erney P. Camargo ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Phytophagous Insects ◽  
Specific Sequence ◽  
Spliced Leader

scholarly journals Novel Type of Staphylococcal Cassette Chromosome mec Identified in Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains

2002 ◽  
Vol 46 (4) ◽  
pp. 1147-1152 ◽  
Author(s):  
Xiao Xue Ma ◽  
Teruyo Ito ◽  
Chuntima Tiensasitorn ◽  
Mantana Jamklang ◽  
Piriyaporn Chongtrakool ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance Genes ◽  
Gene Complex ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Class B ◽  
New Type ◽  
Mrsa Strains ◽  
Hospital Acquired ◽  
Staphylococcal Cassette Chromosome

ABSTRACT We identified a new type of staphylococcal cassette chromosome mec (SCCmec) from two community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. The novel element, designated type IV SCCmec, had a unique combination of the class B mec gene complex and the type 2 ccr gene complex and was much smaller in size (21 to 24 kb) than previously identified SCCmec elements of hospital-acquired MRSA. Consistent with the strains' susceptibilities to various non-β-lactam antibiotics, the type IV SCCmec was devoid of any antibiotic resistance genes other than the mecA gene.

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