scholarly journals Detection and Characterization of PCR-SSCP Markers of the Bovine Lactoferrin Gene for Clinical Mastitis

2006 ◽  
Vol 19 (10) ◽  
pp. 1399-1403 ◽  
Author(s):  
Zhou Lei ◽  
Yuan-Yuan Yang ◽  
Zhong-Hao Li ◽  
Li-Juan Kong ◽  
Guan-Dong Xing ◽  
...  
Keyword(s):  
Clinical Mastitis ◽  
Bovine Lactoferrin ◽  
Lactoferrin Gene

Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle

2015 ◽  
Vol 18 (3) ◽  
pp. 465-471 ◽  
Author(s):  
A. Chopra ◽  
I.D. Gupta ◽  
A. Verma ◽  
A.K. Chakravarty ◽  
V. Vohra
Keyword(s):  
Regulatory Region ◽  
Gene Promoter ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
North India ◽  
Bovine Lactoferrin ◽  
Crossbred Cattle ◽  
Single Nucleotide ◽  
Lactoferrin Gene ◽  
Karan Fries

Abstract Lactoferrin (Lf) gene promoter was screened for the presence of single nucleotide polymphism in indigenous and crossbred cattle from North India and to evaluate its association with Mastitis. Study revealed the presence of genetic variation in regulatory region of bovine Lactoferrin gene using PCR-RFLP technique. Three genotypes namely GG, GH and HH were identified. A single nucleotide change, from guanine to adenine at 25th position was found to be significantly associated (p<0.05) with clinical mastitis in indigenous Sahiwal and crossbred Karan Fries cattle maintained at organised herd of National Dairy Research Institute, Karnal. A non-significant association was observed between subclinical mastitis, somatic cell score (SCS), and GG genotype in Karan Fries cattle, however, a lower SCS was observed in animals having GG genotype. Overall a lower incidence of clinical mastitis was recorded in those animals having GG genotype of Lf in Sahiwal and Karan Fries (KF) cattle. The SNP identified in the promoter region may effect expression lactoferrin protein, which may lead to different levels of antibacterial and anti-inflammatory activity of Lf gene. Results from this study indicated the probable role played by Lactoferrin promoter to serve as candidate gene for mastitis susceptibility among indigenous and crossbred milch cattle.

scholarly journals Characterization of Streptococcus lutetiensis isolated from clinical mastitis of dairy cows

2021 ◽  
Vol 104 (1) ◽  
pp. 702-714
Author(s):  
Peng Chen ◽  
Yun Qiu ◽  
Gang Liu ◽  
Xi Li ◽  
Jia Cheng ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Clinical Mastitis

Characterization of the infection-responsive bovine lactoferrin promoter

Gene ◽  
2005 ◽  
Vol 353 (1) ◽  
pp. 107-117 ◽  
Author(s):  
Jiamao Zheng ◽  
Jennifer L. Ather ◽  
Tad S. Sonstegard ◽  
David E. Kerr
Keyword(s):  
Bovine Lactoferrin

Bovine lactoferrin and its functions in animals -A review

2015 ◽  
Vol 36 (4) ◽  
Author(s):  
Rajan Sharma ◽  
Dibyendu Chakraborty ◽  
Parul Gupta
Keyword(s):  
Bos Taurus ◽  
Bovine Milk ◽  
Regulatory Elements ◽  
Bovine Lactoferrin ◽  
Milk Whey ◽  
Lactoferrin Gene ◽  
Exon 1 ◽  
Variable Content ◽  
Differential Gene ◽  
Strong Antimicrobial Activity

Lactoferrin (Lf) was discovered in 1939 as “red protein from milk whey”. Bovine lactoferrin (bLf) gene is located on <italic>Bos taurus</italic> autosome, long arm of chromosome no.22 (BTA 22q24) in cattle. Its size varies from 23-35kbp among different species. The lactoferrin gene consists of 17 exons and 16 introns ranging from 82bp (exon-1) to 225bp (exon-17). The presence of multiple regulatory elements within lactoferrin promoter contributes differential gene expression and variable content of lactoferrin in milk. The concentration of lactoferrin in normal bovine milk is about 0.02-0.2 mg/ml. The primary function of Lf lies in its role in iron metabolism including iron transport, storage and chelation. Lf exhibits strong antimicrobial activity against a broad spectrum of bacteria (gram-positive & negative), fungi, yeasts, viruses and parasites. Lf exerts bacteriostatic and bactericidal activity. Its main contribution to antiviral defence consists in its binding to the cell membrane glycosaminoglycan, thus lactoferrin prevents viruses from entering cells and infection is stopped at an early age. More than 140 SNPs in this gene have been identiûed. Such a high variability in Lactoferrin gene implies that it may be used as candidate gene for screening animals also a marker of milk yield.

Molecular characterization of lactoferrin gene and its association with mastitis in crossbred cattle

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
Vaibhav Patel ◽  
Bharat Bhushan ◽  
Vishnu Kumar ◽  
Manjit Panigrahi ◽  
Sanjeev Ranjan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Genomic Dna ◽  
Least Square ◽  
Crossbred Cattle ◽  
Cloning And Sequencing ◽  
Brown Swiss ◽  
Lactoferrin Gene ◽  
Exon 4

Genomic DNA was isolated from the blood of 100 crossbred cattle (HF, Jersey, Brown Swiss x Hariana cattle). Exon 4 and intron 6 of lactoferrin (Lf) gene was characterized by PCR-SSCP. Two alleles of exon 4, namely E & F showing two genotypes EE and EF and two alleles of intron 6, namely A and B showing two genotypes AA and AB were found in studied population. Cloning and sequencing results revealed that in exon 4, there was one SNP in allele E and two SNPs in allele F, which changed amino acid isoleucine to valine. In intron 6 there was one SNP in allele A and three SNPs in allele B. Least square analysis for SCC and FLMY revealed that genotype AA was significantly associated with low SCC, while genotype AB was significantly associated with high SCC. However, genotype EE was significantly associated with FLMY as compared to genotype EF.

Characterization of promoter region of CD14 gene and association with clinical mastitis in Karan Fries cattle

2014 ◽  
Vol 48 (6) ◽  
pp. 545 ◽  
Author(s):  
A. Sakthivel Selvan ◽  
I.D. Gupta ◽  
Archana Verma ◽  
Mahesh V. Chaudhari ◽  
Shishir Kanungo ◽  
...  
Keyword(s):  
Promoter Region ◽  
Clinical Mastitis ◽  
Karan Fries ◽  
Cd14 Gene

Molecular Characterization of Multidrug-ResistantEscherichia coliIsolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia

2018 ◽  
Vol 24 (1) ◽  
pp. 95-103 ◽  
Author(s):  
Dalibor Todorović ◽  
Maja Velhner ◽  
Edita Grego ◽  
Dejan Vidanović ◽  
Dubravka Milanov ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Mastitis ◽  
Vojvodina Province
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