scholarly journals Effect of storage time and the level of formic acid on fermentation characteristics, epiphytic microflora, carbohydrate components and in vitro digestibility of rice straw silage

2021 ◽  
Vol 34 (6) ◽  
pp. 1038-1048
Author(s):  
Jie Zhao ◽  
Siran Wang ◽  
Zhihao Dong ◽  
Junfeng Li ◽  
Yushan Jia ◽  
...  
Keyword(s):  
Formic Acid ◽  
Rice Straw ◽  
Dry Matter ◽  
Storage Time ◽  
Gas Production ◽  
Water Soluble ◽  
In Vitro Digestibility ◽  
Neutral Detergent Fibre ◽  
Carbohydrate Components

Objective: The study aimed to evaluate the effect of storage time and formic acid (FA) on fermentation characteristics, epiphytic microflora, carbohydrate components and <i>in vitro</i> digestibility of rice straw silage.Methods: Fresh rice straw was ensiled with four levels of FA (0%, 0.2%, 0.4%, and 0.6% of fresh weight) for 3, 6, 9, 15, 30, and 60 d. At each time point, the silos were opened and sampled for chemical and microbial analyses. Meanwhile, the fresh and 60-d ensiled rice straw were further subjected to <i>in vitro</i> analyses.Results: The results showed that 0.2% and 0.6% FA both produced well-preserved silages with low pH value and undetected butyric acid, whereas it was converse for 0.4% FA. The populations of enterobacteria, yeasts, moulds and aerobic bacteria were suppressed by 0.2% and 0.6% FA, resulting in lower dry matter loss, ammonia nitrogen and ethanol content (p<0.05). The increase of FA linearly (p<0.001) decreased neutral detergent fibre and hemicellulose, linearly (p<0.001) increased residual water soluble carbohydrate, glucose, fructose and xylose. The <i>in vitro</i> gas production of rice straw was decreased by ensilage but the initial gas production rate was increased, and further improved by FA application (p<0.05). No obvious difference of FA application on <i>in vitro</i> digestibility of dry matter, neutral detergent fibre, and acid detergent fibre was observed (p>0.05).Conclusion: The 0.2% FA application level promoted lactic acid fermentation while 0.6% FA restricted all microbial fermentation of rice straw silages. Rice straw ensiled with 0.2% FA or 0.6% FA improved its nutrient preservation without affecting digestion, with the 0.6% FA level best.

scholarly journals Comparison Effects of Ruminal Crabtree-Negative Yeasts and Crabtree-Positive Yeasts for Improving Ensiled Rice Straw Quality and Ruminal Digestion Using In Vitro Gas Production

2020 ◽  
Vol 6 (3) ◽  
pp. 109 ◽  
Author(s):  
Chanon Suntara ◽  
Anusorn Cherdthong ◽  
Suthipong Uriyapongson ◽  
Metha Wanapat ◽  
Pin Chanjula
Keyword(s):  
Rice Straw ◽  
Dry Matter ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Control Group ◽  
In Vitro Digestibility ◽  
Pichia Kudriavzevii ◽  
Yeast Strains ◽  
In Vitro Gas Production

The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility.

Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

scholarly journals The Impact of Ammoniation Treatment on the Chemical Composition and In Vitro Digestibility of Rice Straw in Chinese Holsteins

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1854
Author(s):  
Yulin Ma ◽  
Xu Chen ◽  
Muhammad Zahoor Khan ◽  
Jianxin Xiao ◽  
Shuai Liu ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutritive Value ◽  
Corn Steep Liquor ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
The Impact

The current study was conducted to explore the ammoniation treatment effects on the chemical composition and in vitro digestibility of rice straw in Chinese Holsteins. For this purpose, rice straw was stored in polyethylene bags (35 × 25 cm, 350 g per bag) including (i) no additives (RS); (ii) 5% urea (5U, dry matter (DM) basis); (iii) 9% corn steep liquor + 5% urea (9C5U, DM basis); (iv) 9C2.5U; and (v) 9C2.5U + 3% molasses (9C2.5U3M, DM basis). The air-dry matter of the mixture was kept at the same level at 55% for all treatments. Fifteen bags (5 treatments × 3 repeats) were prepared and stored at ambient temperature (25 ± 3 °C). The chemical composition and in vitro digestibility were measured at day 60 after storage. Our analysis revealed that all the four ammoniation treatments improved the in vitro DM and neutral detergent fiber (IVNDFD) digestibility. In addition, all the four ammoniation treatments significantly (P < 0.001) increased the levels of crude protein (CP), gas production (GP), acetic acid (AA), butyric acid (BA) and total volatile fatty acid (TVFA) contents of the rice straw and decreased the neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the rice straw compared to the control. Within four treated groups, the 9C5U treatment was most effective. Finally, we concluded that ammoniation treatments increased the nutritive value of rice straw. In addition the 9C5U treatment could be an effective ammoniation treatment for the better utilization of rice straw.

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

scholarly journals Nutrient Quality and in Vitro Digestibility of Bio-Ammoniated Rice Straw Bales

2020 ◽  
Vol 44 (3) ◽  
Author(s):  
Cuk Tri Noviandi ◽  
Dibya Ratnopama ◽  
Ali Agus ◽  
Ristianto Utomo
Keyword(s):  
Rice Straw ◽  
Crude Protein ◽  
Production Method ◽  
Proximate Analysis ◽  
Gas Production ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Nutrient Quality ◽  
Randomized Design

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.

scholarly journals Effect of Substitution of Urea With Different Type and Levels of Ruminant Manure on Nutritive Value of Rice Straw Silage

2019 ◽  
Vol 9 (2) ◽  
pp. 206-214
Author(s):  
Ali Ameen Saeed ◽  
Saja Intisar Abid
Keyword(s):  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutritive Value ◽  
In Vitro Digestibility ◽  
N Content ◽  
Aerobic Stability

This study was conducted in Nutrition Lab. to investigate the effect of the type and level of substitution of urea with ruminant manure, M (sheep, cow and buffalo) on basis of nitrogen (N) content on the nutritive value of rice straw silage (RSS). Accordingly silages were nominated as, S-RSS, C-RSS and B-RSS. Urea (U) was substituted with dried manure at 6 combinations, 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50 of U:M. Silage samples were prepared by treating chopped straw with pre-treated solution contained 10% low quality debis and 2% urea. Results showed that lower (P˂0.01) DM loss (11.4%) was observed in S-RSS, and with addition of urea only (3.6%).        Samples of S-RSS and C-RSS recorded higher (P˂0.01) Fleig points (Fp) as compared with those prepared by the addition of B-RSS, 60.42, 55.58 and 49.59 respectively. Reduction (P˂0.01) in this parameter was noticed in samples prepared with a combination of 100:0. Aerobic stability (AS) was a reduced (P˂0.01) in samples prepared by addition of S-RSS by 15 and 13 hours in comparison with samples of C-RSS and B-RSS respectively. Samples prepared with combination of 100:0 were prior (P˂0.01) as compared with other samples.        Results also showed an increase (P˂0.01) in in vitro digestibility of organic matter (IVOMD) in samples of S-RSS in comparison with samples prepared by addition of C-RSS and B-RSS, 49.99, 44.59 and 42.77% respectively. Samples prepared with combination of 100:0 recorded lower (P˂0.05) in vitro digestibility of dry matter (IVDMD) as compared with combinations of 70:30 and 60:40 of U: M, 40.52, 45.36 and 45.94% respectively.

scholarly journals Chemical composition and ruminal degradation kinetics of white oat (Avena sativa L.) IPR 126

2017 ◽  
Vol 18 (1) ◽  
pp. 50-61 ◽  
Author(s):  
Silvonei Tiago RICACHESKI ◽  
Douglas Sampaio HENRIQUE ◽  
Lilian Regina Rothe MAYER ◽  
Jhone Gleison de OLIVEIRA ◽  
Jucemara Aparecida ROSLER ◽  
...  
Keyword(s):  
Avena Sativa ◽  
Dry Matter ◽  
Degradation Kinetics ◽  
Gas Production ◽  
Repeated Measurements ◽  
Chemical Components ◽  
In Vitro Digestibility ◽  
Estimation Of Parameters ◽  
Ruminal Degradation

SUMMARY The present study aiming to determine the nutritional quality of oat (Avena sativa L.) IPR 126 in order to produce forage for ruminants. Four periods between harvests were used: 14, 21, 28 and 35 days, distributed in randomized blocks with four replicates for each treatment. The variables evaluated were: the concentration of dry matter (DM), neutral detergent insoluble fiber (NDF), crude potein (CP), lignin, dry matter (DM), ash, ether extract (EE) and in vitro digestibility of dry matter obtained by measurement of gas production. The profile of gas production was adjusted to the logistic bicompartimental mathematical model. The variables and the parameters of the adjusted gas production curves were analyzed as repeated measurements through the PROC MIXED of SAS (version 9.0) and the restricted maximum likelihood (REML) as the method of estimation of parameters. Regression analysis was performed for the variables: DM, CP, EE, NDF, ash, and for the parameter k2 of the bicompartimental model. DM and NDF concentrations increased linearly, CP, ash, and the estimations of the parameter k2 reduced linearly and the EE concentration showed a cubic behavior in function of the age of harvest. Lignin and other parameters of the Schofield model were not influenced by the age of harvest. The harvest interval influenced some chemical components and degradation rate of fiber carbohydrates, but do not interfere in lignin concentration. The forage with 21 days of cutting interval has the high nutritional value.

Single-cut red clover combined with late timothy may be valuable in short-term rotations

2000 ◽  
Vol 80 (2) ◽  
pp. 309-313 ◽  
Author(s):  
H. T. Kunelius ◽  
K. B. McRae ◽  
S. A. E. Fillmore ◽  
G. Dürr
Keyword(s):  
Dry Matter ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Phleum Pratense ◽  
Water Soluble ◽  
Nutrient Concentrations ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Short Term

Late-maturing cultivars of red clover (Trifolium pratense L.) and timothy (Phleum pratense L.) may be grown in short-term rotations with other crops and harvested for hay and silage. Harvesting forage in mid-summer is often advantageous for field curing, but the quality of mature forage may be low. We studied how harvests at different developmental stages affected yield, composition of herbage, and species persistence. The late-maturing single-cut red clover cultivar Altaswede and late timothy cultivar Farol were grown alone and in combination with and without applied N. Total forage, and red clover and timothy dry matter yields, increased between the first (27 June) and third (11 July) dates of harvest. Timothy grown alone or combined with red clover and fertilized with N produced greater forage yields than red clover alone or red clover + timothy without applied N. The in vitro digestibility of dry matter declined from 670 to 625 g kg−1 and crude protein from 134 to 109 g kg−1 between 27 June and 11 July. Water soluble carbohydrates in forage were low (36–66 g kg−1) in cuts 1 and 2. Macronutrient and micronutrient concentrations were generally highest at the first date of harvest on 27 June. Nutrient concentrations in cut 2 were similar for the three harvest schedules. We conclude that growing late-maturing single-cut red clover combined with late timothy produced high yields with good nutritional quality even at advanced stages of maturity. Single-cut red clover persisted for the first production year making this combination suited for short-term rotations that involve late harvesting of the primary growth. Key words: Red clover, Trifolium pratense, timothy, Phleum pratense, composition

Prediction of Digestibility and Intake of Sheep Fed Leguminosae or GRAMINAE Hays: Comparison Between the in Vitro Digestibility, Kinetics of Gas Production or Dry Matter Degradation

1994 ◽  
Vol 1994 ◽  
pp. 103-103
Author(s):  
M.T. Dentinho ◽  
K. Khazaal ◽  
J.M. Ribeiro ◽  
E.R. Ørskov
Keyword(s):  
Dry Matter ◽  
Lolium Multiflorum ◽  
Daily Intake ◽  
Gas Production ◽  
Italian Ryegrass ◽  
In Vitro Digestibility ◽  
Kinetics Of

By using separated values of kinetics of in situ dry matter (DM) degradation or in vitro gas production (Menke and Steingass, 1988) of leguminosae hays, Khazaal et al, (1993) reported high correlation with intake (r= 0.88; r= 0.79) and in vivo DM digestibility (DMD) (r= 0.94; r= 0.88). The aim of the present study was to extend the range of samples used and compare the ability of the 2 stages in vitro digestibility (Tilley and Terry, 1963), the in situ DM degradation or the gas production techniques to predict daily intake (g DM/ kgW0.75) and in vivo DM digestibility (DMD) of 19 leguminous and graminaceous hays fed to sheep.Three harvesting stages (early bloom EB, mid bloom MB or in seed IS) made from lucerne (Medicago sativa), sweet clover (Melilotus segetalis), Persian clover (Trifolium resupinatum), Rye (Secale cereale), Triticale (Triticale hexaploid), oat (Avena stativa) and a pre-bloom (PB) Italian ryegrass (Lolium multiflorum ). Each hay was fed ad libitum to 4 Merino male sheep and their intake and in vivo DMD recorded. Gas production (ml/ 200 mg DM) or in situ DM degradation (g/ 100 g DM) were determined as described by Khazaal et al, (1993) after 6, 12, 24, 48, 72 or 96 h incubation. Measured gas production or DM degradation values were fitted to the equation p=a+b(l-e-ct)(McDonald, 1981) where p is gas production or DM degradation at time t and a, b and c are constants. For nylon bag the washing loss (soluble fraction) was defined as A, the insoluble but fermentable matter was defined as B=(a+b)-A, and c is the rate of fermentation or degradation (Ørskov and Ryle, 1990).

scholarly journals Mixed sugarcane and elephant grass silages with or without bacterial inoculant

2010 ◽  
Vol 39 (3) ◽  
pp. 462-470 ◽  
Author(s):  
Jucilene Cavali ◽  
Odilon Gomes Pereira ◽  
Sebastião de Campos Valadares Filho ◽  
Marlos Oliveira Porto ◽  
Francisco Eden Paiva Fernandes ◽  
...  
Keyword(s):  
Dry Matter ◽  
Linear Models ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Elephant Grass ◽  
Acid Detergent Fiber ◽  
Total N

The effects of different ratios of sugarcane and elephant grass (0:100, 25:75, 50:50, 75:25 and 100:0% of the natural basis) were assessed on the chemical composition and losses in silages treated with a bacterial inoculant, using laboratory silos. A 2 × 5 factorial arrangement (with and without inoculant and five elephant grass ratios) in a randomized blocks design with three replications was used. Interaction was observed in the sugarcane and elephant grass ratio × bacterial inoculant for crude protein (CP) and pH. The other variables were influenced only by the increasing proportions of elephant grass. The contents of dry matter, neutral detergent fiber corrected for ashes and protein, acid detergent fiber, insoluble protein in acid detergent fiber, hemicellulose and lignin in the silages increased linearly with the proportions of elephant grass. The water soluble carbohydrate contents and dry matter in vitro digestibility of the silages decreased linearly with the increase in the proportion of elephant grass. The mean value of ammonia nitrogen in relation to total N was 7.0% (% of DM). The CP values of the inoculated and non-inoculated silage fitted linear models. The highest CP content was observed in the silage treated with inoculant. The pH values of the silages, with and without inoculant, fitted quadratic and linear models, respectively. The lactic acid, propionic acid and butyric acid contents were not influenced by the elephant grass ratios, while the acetic acid content, for the non-inoculated silages, and ethanol decreased linearly with the increase in elephant grass. The ratio of 25% sugarcane and 75% elephant grass improves the nutritional value and increases the silage DM recovery, because of the lower effluent and gas productions. The homofermentative bacterial inoculant does not affect the sugarcane silage.

Sign in / Sign up

Export Citation Format

Share Document