scholarly journals Metabolomics comparison of rumen fluid and milk in dairy cattle using proton nuclear magnetic resonance spectroscopy

2021 ◽  
Vol 34 (2) ◽  
pp. 213-222
Author(s):  
Jun Sik Eom ◽  
Eun Tae Kim ◽  
Hyun Sang Kim ◽  
You Young Choi ◽  
Shin Ja Lee ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Dairy Cattle ◽  
Metabolic Diseases ◽  
Rumen Fluid ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Nuclear Magnetic

Objective: The metabolites that constitute the rumen fluid and milk in dairy cattle were analyzed using proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy and compared with the results obtain for other dairy cattle herds worldwide. The aim was to provide basic dataset for facilitating research on metabolites in rumen fluid and milk.Methods: Six dairy cattle were used in this study. Rumen fluid was collected using a stomach tube, and milk was collected using a pipeline milking system. The metabolites were determined by <sup>1</sup>H-NMR spectroscopy, and the obtained data were statistically analyzed by principal component analysis, partial least squares discriminant analysis, variable importance in projection scores, and metabolic pathway data using Metaboanalyst 4.0.Results: The total numbers of metabolites in rumen fluid and milk were measured to be 186 and 184, and quantified as 72 and 109, respectively. Organic acid and carbohydrate metabolites exhibited the highest concentrations in rumen fluid and milk, respectively. Some metabolites that have been associated with metabolic diseases (acidosis and ketosis) in cows were identified in rumen fluid, and metabolites associated with ketosis, somatic cell production, and coagulation properties were identified in milk.Conclusion: The metabolites measured in rumen fluid and milk could potentially be used to detect metabolic diseases and evaluate milk quality. The results could also be useful for metabolomic research on the biofluids of ruminants in Korea, while facilitating their metabolic research.

scholarly journals Metabolic Profiling of Rumen Fluid and Milk in Lactating Dairy Cattle Influenced by Subclinical Ketosis Using Proton Nuclear Magnetic Resonance Spectroscopy

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2526
Author(s):  
Jun-Sik Eom ◽  
Hyun-Sang Kim ◽  
Shin-Ja Lee ◽  
You-Young Choi ◽  
Seong-Uk Jo ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dairy Cattle ◽  
Metabolic Diseases ◽  
Rumen Fluid ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Subclinical Ketosis ◽  
Lactating Dairy Cattle ◽  
Nuclear Magnetic

Ketosis metabolic research on lactating dairy cattle has been conducted worldwide; however, there have been very few Korean studies. Biofluids from lactating dairy cattle are necessary to study ketosis metabolic diseases. Six Holstein cows were divided into two groups (healthy (CON) and subclinical ketosis diagnosed (SCK)). Rumen fluid and milk samples were collected using a stomach tube and a pipeline milking system, respectively. Metabolites were determined using proton nuclear magnetic resonance (NMR) spectroscopy and they were identified and quantified using the Chenomx NMR Suite 8.4 software and Metaboanalyst 5.0. In the rumen fluid of the SCK group, butyrate, sucrose, 3-hydroxybutyrate, maltose, and valerate levels were significantly higher than in the CON group, which showed higher levels of N,N-dimethylformamide, acetate, glucose, and propionate were significantly higher. Milk from the SCK group showed higher levels of maleate, 3-hydroxybutyrate, acetoacetate, galactonate, and 3-hydroxykynurenine than that from the CON group, which showed higher levels of galactitol, 1,3-dihydroxyacetone, γ-glutamylphenylalanine, 5-aminolevulinate, acetate, and methylamine. Some metabolites are associated with ketosis diseases and the quality of rumen fluid and milk. This report will serve as a future reference guide for ketosis metabolomics studies in Korea.

Serum analysis by1H Nuclear Magnetic Resonance spectroscopy: a new tool for distinguishing neuromyelitis optica from multiple sclerosis

2013 ◽  
Vol 20 (5) ◽  
pp. 558-565 ◽  
Author(s):  
F-M Moussallieh ◽  
K Elbayed ◽  
JB Chanson ◽  
G Rudolf ◽  
M Piotto ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Neuromyelitis Optica ◽  
Demyelinating Diseases ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Nuclear Magnetic

Background:Neuromyelitis optica (NMO) and multiple sclerosis (MS), two inflammatory demyelinating diseases, are characterized by different therapeutic strategies. Currently, the only biological diagnostic tool available to distinguish NMO from MS is the specific serum autoantibody that targets aquaporin 4, but its sensitivity is low.Objective:To assess the diagnostic accuracy of metabolomic biomarker profiles in these two neurological conditions, compared to control patients.Methods:We acquired serum spectra (47 MS, 44 NMO and 42 controls) using proton nuclear magnetic resonance (1H-NMR) spectroscopy. We used multivariate pattern recognition analysis to identify disease-specific metabolic profiles.Results:The1H-NMR spectroscopic analysis evidenced two metabolites, originating probably from astrocytes, scyllo-inositol and acetate, as promising serum biomarkers of MS and NMO, respectively. In 87.8% of MS patients, scyllo-inositol increased 0.15 to 3-fold, compared to controls and in 74.3% of NMO patients, acetate increased 0.4 to 7-fold, compared to controls. Using these two metabolites simultaneously, we can discriminate MS versus NMO patients (sensitivity, 94.3%; specificity, 90.2%).Conclusion:This study demonstrates the potential of1H-NMR spectroscopy of serum as a novel, promising analytical tool to discriminate populations of patients affected by NMO or MS.

Proton nuclear magnetic resonance spectroscopy of body fluids in the field of inborn errors of metabolism

2003 ◽  
Vol 40 (1) ◽  
pp. 16-24 ◽  
Author(s):  
S.H. Moolenaar ◽  
U.F.H. Engelke ◽  
R.A. Wevers
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Inborn Errors Of Metabolism ◽  
Metabolic Diseases ◽  
Body Fluids ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Inborn Errors ◽  
Nuclear Magnetic

Proton nuclear magnetic resonance (NMR) spectroscopy of body fluids has been successfully applied to the field of inborn errors of metabolism. This technique has the advantage of minimal sample pretreatment not requiring extraction or derivatization steps. Moreover, the spectrum provides a comprehensive metabolic profile of proton-containing, low-molecular-weight metabolites. The sensitivity limit is in the low micromolar range. This allows diagnosis of many inborn errors of metabolism. This review explains the key features of the NMR spectrum and reviews the available literature on metabolic diseases. Three novel diseases have been delineated with the technique. Relevant parts of the spectra from the urine samples of patients with these diseases are shown. NMR spectroscopy may develop to become a key tool in a metabonomics approach in clinical biochemistry.

Use of high-resolution proton nuclear magnetic resonance spectroscopy for rapid multi-component analysis of urine.

1984 ◽  
Vol 30 (3) ◽  
pp. 426-432 ◽  
Author(s):  
J R Bales ◽  
D P Higham ◽  
I Howe ◽  
J K Nicholson ◽  
P J Sadler
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
High Resolution ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Simple Method ◽  
Nuclear Magnetic

Abstract Numerous low-Mr metabolites--including creatinine, citrate, hippurate, glucose, ketone bodies, and various amino acids--have been identified in 400- and 500-MHz proton nuclear magnetic resonance (1H NMR) spectra of intact human urine. The presence of many of these was related to the specific condition of the donors: humans in different physiological states (resting, fasting, or post-exercise) and pathological conditions (e.g., diabetes mellitus, cadmium-induced renal dysfunction). We have also monitored the metabolism of simple nonendogenous compounds (methanol and ethanol) and of acetaminophen. The pH-dependencies of the NMR chemical shifts of some urine components are reported. Our studies show that high-resolution 1H NMR spectroscopy provides a fast, simple method for "fingerprint" identification of urinary compounds. In some cases, analytes can be quantified by standard additions or by comparing integrated peak areas for the metabolites with those for creatinine. Determinations of creatinine by 1H NMR spectroscopy compared well with those by an independent chemical assay based on the Jaffé reaction.

scholarly journals 1H Nuclear Magnetic Resonance: A Future Approach to the Metabolic Profiling of Psychedelics in Human Biofluids?

2021 ◽  
Vol 12 ◽  
Author(s):  
Sylvana Vilca-Melendez ◽  
Malin V. Uthaug ◽  
Julian L. Griffin
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Metabolic Profiling ◽  
Proton Nuclear Magnetic Resonance ◽  
Future Directions ◽  
H Nmr ◽  
Nuclear Magnetic ◽  
Psychedelic Research

While psychedelics may have therapeutic potential for treating mental health disorders such as depression, further research is needed to better understand their biological effects and mechanisms of action when considering the development of future novel therapy approaches. Psychedelic research could potentially benefit from the integration of metabonomics by proton nuclear magnetic resonance (1H NMR) spectroscopy which is an analytical chemistry-based approach that can measure the breakdown of drugs into their metabolites and their metabolic consequences from various biofluids. We have performed a systematic review with the primary aim of exploring published literature where 1H NMR analysed psychedelic substances including psilocin, lysergic acid diethylamide (LSD), LSD derivatives, N,N-dimethyltryptamine (DMT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) and bufotenin. The second aim was to assess the benefits and limitations of 1H NMR spectroscopy-based metabolomics as a tool in psychedelic research and the final aim was to explore potential future directions. We found that the most current use of 1H NMR in psychedelic research has been for the structural elucidation and analytical characterisation of psychedelic molecules and that no papers used 1H NMR in the metabolic profiling of biofluids, thus exposing a current research gap and the underuse of 1H NMR. The efficacy of 1H NMR spectroscopy was also compared to mass spectrometry, where both metabonomics techniques have previously shown to be appropriate for biofluid analysis in other applications. Additionally, potential future directions for psychedelic research were identified as real-time NMR, in vivo1H nuclear magnetic resonance spectroscopy (MRS) and 1H NMR studies of the gut microbiome. Further psychedelic studies need to be conducted that incorporate the use of 1H NMR spectroscopy in the analysis of metabolites both in the peripheral biofluids and in vivo to determine whether it will be an effective future approach for clinical and naturalistic research.

scholarly journals Paraquat-induced renal injury studied by 1H nuclear magnetic resonance spectroscopy of urine

1998 ◽  
Vol 44 (6) ◽  
pp. 1256-1261 ◽  
Author(s):  
Eleni Bairaktari ◽  
Kostas Katopodis ◽  
Kostas C Siamopoulos ◽  
Orestes Tsolas
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
1H Nmr ◽  
Renal Injury ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Renal Tubules ◽  
Endogenous Metabolites ◽  
Nuclear Magnetic

Abstract The herbicide paraquat (1,1′-dimethyl-4,4′-bipyridylium dichloride; PQ), is a poison known to cause delayed mortality due to lung and kidney injuries. High-resolution proton nuclear magnetic resonance (1H NMR) spectroscopy has been extensively applied in evaluating nephrotoxicity by the characteristic perturbations in the excretion pattern of low molecular weight endogenous metabolites. The application of the method allows the rapid localization of the renal injury noninvasively. In this study, we report 1H NMR and conventional clinical chemistry urinalysis in two patients suffering from paraquat intoxication after overdose with suicidal intent. The alterations in the urine NMR spectrum suggest necrosis of the pars recta of the proximal renal tubules. The molecule of paraquat is also clearly detected in the same spectrum. In conclusion, the rapid screening of urine by NMR spectroscopy provides information about both the identity of the poison and the abnormal pattern of endogenous metabolites that characterize the location of the injury in renal tubules and reveals alterations in unusual metabolites that are not commonly measured.

Analysis of volatile fatty acids in rumen fluid by proton NMR spectroscopy

1999 ◽  
Vol 79 (3) ◽  
pp. 401-404 ◽  
Author(s):  
M. A. Attaelmannan ◽  
A. A. Dahl ◽  
R. S. Reid
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Magnetic Resonance ◽  
Volatile Fatty Acid ◽  
Rumen Fluid ◽  
Spectroscopic Method ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Nuclear Magnetic

A study was conducted to quantify the volatile fatty acid (VFA) concentrations in aqueous and rumen fluid samples using a standard additions based quantitative proton nuclear magnetic resonance (NMR) spectroscopic method. Standard plots for acetic, propionic and butyric acids were all found to be linear (R2 > 0.99). Results obtained by NMR were compared with those obtained using the conventional gas chromatographic method. No significant differences were found between the means and precisions of the two techniques (P < 0.05). The method is well-suited for the routine quantification of these acids, requires no sample pretreatment and is more rapid than gas chromatography. Key words: Volatile fatty acid determination, rumen fluid, gas chromatography, nuclear magnetic resonance spectroscopy

scholarly journals Metabolomics Comparison of Hanwoo (Bos taurus coreanae) Biofluids Using Proton Nuclear Magnetic Resonance Spectroscopy

Metabolites ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 333
Author(s):  
Jun Sik Eom ◽  
Shin Ja Lee ◽  
Hyun Sang Kim ◽  
You Young Choi ◽  
Sang Ho Kim ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Bos Taurus ◽  
Rumen Fluid ◽  
Relevant Information ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Metabolic Pathway Analysis ◽  
Serum And Urine ◽  
Nuclear Magnetic

The aim of this study was to identify the metabolomic profiles of rumen fluid, serum, and urine from Hanwoo (Bos taurus coreanae), using proton nuclear magnetic resonance (1H-NMR) spectroscopy. In all, 189, 110, and 188 metabolites were identified in rumen fluid, serum, and urine, and 107, 49, and 99 were quantified, respectively. Organic acids, carbohydrates, and aliphatic acyclic compound metabolites were present at the highest concentrations in rumen fluid, serum, and urine, respectively. In addition, acetate, glucose, and urea were the most highly concentrated individual metabolites in rumen fluid, serum, and urine, respectively. In all, 77 metabolites were commonly identified, and 19 were quantified across three biofluids. Metabolic pathway analysis showed that the common quantified metabolites could provide relevant information about three main metabolic pathways, phenylalanine, tyrosine, and tryptophan biosynthesis; caffeine metabolism; and histidine metabolism. These results can be useful as reference values for future metabolomic research on Hanwoo biofluids in Korea.

Synthesis and biological activities of maleated rosin-based dithiourea compounds

Holzforschung ◽  
2014 ◽  
Vol 68 (5) ◽  
pp. 549-554 ◽  
Author(s):  
Gui-Shan Lin ◽  
Su-Qiu Dong ◽  
Wen-Gui Duan ◽  
Bo Cen ◽  
Xue-Tang Xu ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Biological Activities ◽  
Herbicidal Activity ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Nuclear Magnetic

Abstract In search of new bioactive compounds, a series of maleated rosin-based dithiourea compounds were designed and synthesised with rosin as starting material. All the title compounds were characterised by Fourier transform infra-red spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (1H NMR), and 13C nuclear magnetic resonance spectroscopy (13C NMR). The preliminary bioassay showed that the target compounds exhibited a certain fungicidal activity against Physalospora piricola at a concentration of 50 μg ml-1 and a certain growth herbicidal activity against the root of rape (Brassica campestris L.) at a concentration of 100 μg ml-1.

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