scholarly journals Metabolic Profiling of Rumen Fluid and Milk in Lactating Dairy Cattle Influenced by Subclinical Ketosis Using Proton Nuclear Magnetic Resonance Spectroscopy

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2526
Author(s):  
Jun-Sik Eom ◽  
Hyun-Sang Kim ◽  
Shin-Ja Lee ◽  
You-Young Choi ◽  
Seong-Uk Jo ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dairy Cattle ◽  
Metabolic Diseases ◽  
Rumen Fluid ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Subclinical Ketosis ◽  
Lactating Dairy Cattle ◽  
Nuclear Magnetic

Ketosis metabolic research on lactating dairy cattle has been conducted worldwide; however, there have been very few Korean studies. Biofluids from lactating dairy cattle are necessary to study ketosis metabolic diseases. Six Holstein cows were divided into two groups (healthy (CON) and subclinical ketosis diagnosed (SCK)). Rumen fluid and milk samples were collected using a stomach tube and a pipeline milking system, respectively. Metabolites were determined using proton nuclear magnetic resonance (NMR) spectroscopy and they were identified and quantified using the Chenomx NMR Suite 8.4 software and Metaboanalyst 5.0. In the rumen fluid of the SCK group, butyrate, sucrose, 3-hydroxybutyrate, maltose, and valerate levels were significantly higher than in the CON group, which showed higher levels of N,N-dimethylformamide, acetate, glucose, and propionate were significantly higher. Milk from the SCK group showed higher levels of maleate, 3-hydroxybutyrate, acetoacetate, galactonate, and 3-hydroxykynurenine than that from the CON group, which showed higher levels of galactitol, 1,3-dihydroxyacetone, γ-glutamylphenylalanine, 5-aminolevulinate, acetate, and methylamine. Some metabolites are associated with ketosis diseases and the quality of rumen fluid and milk. This report will serve as a future reference guide for ketosis metabolomics studies in Korea.

scholarly journals Metabolomics comparison of rumen fluid and milk in dairy cattle using proton nuclear magnetic resonance spectroscopy

2021 ◽  
Vol 34 (2) ◽  
pp. 213-222
Author(s):  
Jun Sik Eom ◽  
Eun Tae Kim ◽  
Hyun Sang Kim ◽  
You Young Choi ◽  
Shin Ja Lee ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Dairy Cattle ◽  
Metabolic Diseases ◽  
Rumen Fluid ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Nuclear Magnetic

Objective: The metabolites that constitute the rumen fluid and milk in dairy cattle were analyzed using proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy and compared with the results obtain for other dairy cattle herds worldwide. The aim was to provide basic dataset for facilitating research on metabolites in rumen fluid and milk.Methods: Six dairy cattle were used in this study. Rumen fluid was collected using a stomach tube, and milk was collected using a pipeline milking system. The metabolites were determined by <sup>1</sup>H-NMR spectroscopy, and the obtained data were statistically analyzed by principal component analysis, partial least squares discriminant analysis, variable importance in projection scores, and metabolic pathway data using Metaboanalyst 4.0.Results: The total numbers of metabolites in rumen fluid and milk were measured to be 186 and 184, and quantified as 72 and 109, respectively. Organic acid and carbohydrate metabolites exhibited the highest concentrations in rumen fluid and milk, respectively. Some metabolites that have been associated with metabolic diseases (acidosis and ketosis) in cows were identified in rumen fluid, and metabolites associated with ketosis, somatic cell production, and coagulation properties were identified in milk.Conclusion: The metabolites measured in rumen fluid and milk could potentially be used to detect metabolic diseases and evaluate milk quality. The results could also be useful for metabolomic research on the biofluids of ruminants in Korea, while facilitating their metabolic research.

Proton nuclear magnetic resonance spectroscopy of body fluids in the field of inborn errors of metabolism

2003 ◽  
Vol 40 (1) ◽  
pp. 16-24 ◽  
Author(s):  
S.H. Moolenaar ◽  
U.F.H. Engelke ◽  
R.A. Wevers
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Inborn Errors Of Metabolism ◽  
Metabolic Diseases ◽  
Body Fluids ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Inborn Errors ◽  
Nuclear Magnetic

Proton nuclear magnetic resonance (NMR) spectroscopy of body fluids has been successfully applied to the field of inborn errors of metabolism. This technique has the advantage of minimal sample pretreatment not requiring extraction or derivatization steps. Moreover, the spectrum provides a comprehensive metabolic profile of proton-containing, low-molecular-weight metabolites. The sensitivity limit is in the low micromolar range. This allows diagnosis of many inborn errors of metabolism. This review explains the key features of the NMR spectrum and reviews the available literature on metabolic diseases. Three novel diseases have been delineated with the technique. Relevant parts of the spectra from the urine samples of patients with these diseases are shown. NMR spectroscopy may develop to become a key tool in a metabonomics approach in clinical biochemistry.

Analysis of volatile fatty acids in rumen fluid by proton NMR spectroscopy

1999 ◽  
Vol 79 (3) ◽  
pp. 401-404 ◽  
Author(s):  
M. A. Attaelmannan ◽  
A. A. Dahl ◽  
R. S. Reid
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Magnetic Resonance ◽  
Volatile Fatty Acid ◽  
Rumen Fluid ◽  
Spectroscopic Method ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Nuclear Magnetic

A study was conducted to quantify the volatile fatty acid (VFA) concentrations in aqueous and rumen fluid samples using a standard additions based quantitative proton nuclear magnetic resonance (NMR) spectroscopic method. Standard plots for acetic, propionic and butyric acids were all found to be linear (R2 > 0.99). Results obtained by NMR were compared with those obtained using the conventional gas chromatographic method. No significant differences were found between the means and precisions of the two techniques (P < 0.05). The method is well-suited for the routine quantification of these acids, requires no sample pretreatment and is more rapid than gas chromatography. Key words: Volatile fatty acid determination, rumen fluid, gas chromatography, nuclear magnetic resonance spectroscopy

scholarly journals Metabolomics Comparison of Hanwoo (Bos taurus coreanae) Biofluids Using Proton Nuclear Magnetic Resonance Spectroscopy

Metabolites ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 333
Author(s):  
Jun Sik Eom ◽  
Shin Ja Lee ◽  
Hyun Sang Kim ◽  
You Young Choi ◽  
Sang Ho Kim ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Bos Taurus ◽  
Rumen Fluid ◽  
Relevant Information ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Metabolic Pathway Analysis ◽  
Serum And Urine ◽  
Nuclear Magnetic

The aim of this study was to identify the metabolomic profiles of rumen fluid, serum, and urine from Hanwoo (Bos taurus coreanae), using proton nuclear magnetic resonance (1H-NMR) spectroscopy. In all, 189, 110, and 188 metabolites were identified in rumen fluid, serum, and urine, and 107, 49, and 99 were quantified, respectively. Organic acids, carbohydrates, and aliphatic acyclic compound metabolites were present at the highest concentrations in rumen fluid, serum, and urine, respectively. In addition, acetate, glucose, and urea were the most highly concentrated individual metabolites in rumen fluid, serum, and urine, respectively. In all, 77 metabolites were commonly identified, and 19 were quantified across three biofluids. Metabolic pathway analysis showed that the common quantified metabolites could provide relevant information about three main metabolic pathways, phenylalanine, tyrosine, and tryptophan biosynthesis; caffeine metabolism; and histidine metabolism. These results can be useful as reference values for future metabolomic research on Hanwoo biofluids in Korea.

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