scholarly journals Dietary lysophospholipids supplementation inhibited the activity of lipolytic bacteria in forage with high oil diet: an in vitro study

2020 ◽  
Vol 33 (10) ◽  
pp. 1590-1598
Author(s):  
Hanbeen Kim ◽  
Byeongwoo Kim ◽  
Seongkeun Cho ◽  
Inhyuk Kwon ◽  
Jakyeom Seo
Keyword(s):  
Microbial Diversity ◽  
Corn Oil ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Annual Ryegrass ◽  
Linear Effect ◽  
Significant Difference

Objective: The objective of this study was to evaluate the effects of lysophospholipids (LPL) supplementation on rumen fermentation, degradability, and microbial diversity in forage with high oil diet in an in vitro system.Methods: Four experimental treatments were used: i) annual ryegrass (CON), ii) 93% annual ryegrass +7% corn oil on a dry matter (DM) basis (OiL), iii) OiL with a low level (0.08% of dietary DM) of LPL (LLPL), and iv) OiL with a high level (0.16% of dietary DM) of LPL (HLPL). An in vitro fermentation experiment was performed using strained rumen fluid for 48 h incubations. In vitro DM degradability (IVDMD), in vitro neutral detergent fiber degradability, pH, ammonia nitrogen (NH3-N), volatile fatty acid (VFA), and microbial diversity were estimated.Results: There was no significant change in IVDMD, pH, NH3-N, and total VFA production among treatments. The LPL supplementation significantly increased the proportion of butyrate and valerate (Linear effect [Lin], p = 0.004 and <0.001, respectively). The LPL supplementation tended to increase the total bacteria in a linear manner (p = 0.089). There were significant decreases in the relative proportions of cellulolytic (Fibrobacter succinogenes and Ruminococcus albus) and lipolytic (Anaerovibrio lipolytica and Butyrivibrio proteoclasticus) bacteria with increasing levels of LPL supplementation (Lin, p = 0.028, 0.006, 0.003, and 0.003, respectively).Conclusion: The LPL supplementation had antimicrobial effects on several cellulolytic and lipolytic bacteria, with no significant difference in nutrient degradability (DM and neutral detergent fiber) and general bacterial counts, suggesting that LPL supplementation might increase the enzymatic activity of rumen bacteria. Therefore, LPL supplementation may be more effective as an antimicrobial agent rather than as an emulsifier in the rumen.

scholarly journals The potential of tropical grass as a feed in ruminant by using an in vitro gas production

2018 ◽  
Vol 197 ◽  
pp. 06005
Author(s):  
Nevyani Asikin ◽  
Anuraga Jayanegara ◽  
Muhamad Ridla ◽  
Anjas Asmara Samsudin
Keyword(s):  
Animal Feed ◽  
Block Design ◽  
In Vitro Study ◽  
Proximate Analysis ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
In Vitro Gas Production ◽  
Significant Difference ◽  
Tropical Grass

Pennisetum purpoides is one of the most widely cultivated tropical forages and it is often used as animal feed in Indonesia. However, grass feeding could not fulfill the need of nutrients if served as single feed because it contained of high crude fiber and low protein. In terms of nutritional adequacy, animal feed should contain complete nutritional value in order to achieve an optimum productivity. This experiment aimed to elucidate the potential of tropical grass as a feed in ruminant by using an in vitro gas production technicque. The grasses were determined for proximate analysis, Van Soest fiber fractions (neutral detergent fiber, acid detergent fiber and acid detergent lignin), and in vitro rumen fermentation parameters. This study was conducted from January until April 2018 at Nutrition Laboratory, Department of Animal Science, Faculty of Agriculture, University Putra Malaysia, Serdang Selangor Darul Ehsan, Malaysia. This study used a randomized block design with 3 replications. There were three treatments tested in this study, i.e. (1) Pennisetum purpoides (T1), (2) Setaria splendida (T2), (3) Setaria anceps (T3). Parameters measured in the in vitro study is total gas production. Data were tested using analysis of variance (ANOVA) and continued with Duncan test if there was a significant difference among treatments. The data showed that gas production is not significantly different (P>0,05).

scholarly journals Effects of Using Mentha pulegium and Ziziphora clinopodioides Essential Oils as Additive on in vitro study

2018 ◽  
Vol 15 (1) ◽  
pp. 217-227
Author(s):  
M. Edalati Nasab ◽  
A.A. Naserian ◽  
A.R. Vakili ◽  
A.M. Tahmasbi
Keyword(s):  
Essential Oils ◽  
Production Rate ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Production Method ◽  
Gas Production ◽  
Mentha Pulegium ◽  
Production Values ◽  
Significant Difference

The purpose of Present study was conducted to evaluate the effects of adding essential oils (Ziziphora clinopodioides and Mentha pulegium) on alfalfa silage on the rumen degradation parameters with in vitro technique. Present study was performed by utilizing an in vitro gas production method at various incubation intervals. Rumen fluid taken from three lactation, rumen-fistulated Holstein cows. The gas production rate was measured at standard times from 0, to 96 hours. The outcomes of this experiment show that Ziziphora clinopodioides and also Mentha pulegium essential oils had a positive influence on gas production rate. Silage content with Mentha pulegium had more decrease effects than Ziziphora clinopodioides in Gas production compare with control silage and it was significant. Gas production values (at 96 h incubation) in silage with no added essential oils, 30ML of Ziziphora clinopodioides and 30ML of Mentha pulegium were 68.82, 56.12 and 49.74, respectively. Compared with control, aerobic stability had a significant difference and it was developed in silage treated with essential oils. The findings of their findings showed that these essential oils could be used to increase the performance of ruminants. In addition, adding essential oils could change the rumen fermentation in ruminant, however, more research is still needed to proving this conclusion.

scholarly journals Effects of Wormwood (Artemisia montana) Essential Oils on Digestibility, Fermentation Indices, and Microbial Diversity in the Rumen

2020 ◽  
Vol 8 (10) ◽  
pp. 1605
Author(s):  
Seong Shin Lee ◽  
Dong Hyeon Kim ◽  
Dimas Hand Vidya Paradhipta ◽  
Hyuk Jun Lee ◽  
Hee Yoon ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Short Chain Fatty Acid ◽  
Rumen Fluid ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Dry Matter Digestibility ◽  
Different Doses ◽  
Better Than

This study investigated the effects of essential oil (EO) from three Korean wormwood (Artemisia Montana) plants on in vitro ruminal digestibility, fermentation, and microbial diversity. Dried (0.5 g) soybean meal (SBM) or bermudagrass hay (BGH) were incubated in buffered rumen fluid (40 mL) for 72 h with or without EO (5 mg/kg) from Ganghwa (GA), Injin (IN), or San (SA) wormwood (Experiment 1). Both SA and IN improved (p < 0.05) dry matter digestibility (DMD) of BGH, while GA reduced (p < 0.05) total short-chain fatty acid of BGH and SBM. Besides, SA increased (p < 0.05) numbers of Ruminococcus albus and Streptococcus bovis in SBM. Experiment 2 examined different doses (0, 0.1, 1, and 10 mg/kg) of SA, the most promising EO from Experiment 1. Applying SA at 10 mg/kg gave the highest DMD (L; p < 0.01) and neutral detergent fiber (Q; p < 0.05) digestibility for BGH. Applying SA at 1 mg/kg gave the highest R. albus population (Q; p < 0.05) in SBM. Therefore, SA was better than GA and IN at improving rumen fermentation, and the 0.1 to 1 and 10 mg/kg doses improved ruminal fermentation and in vitro digestibility of SBM and BGH, respectively.

scholarly journals Elephant grass silages with or without wilting, with cassava meal in silage production

2017 ◽  
Vol 18 (3) ◽  
pp. 417-429 ◽  
Author(s):  
Aline Cardoso OLIVEIRA ◽  
Rasmo GARCIA ◽  
Aureliano José Vieira PIRES ◽  
Hellenn Cardoso OLIVEIRA ◽  
Vitor Visintin Silva de ALMEIDA ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Free Acid ◽  
Organic Matter Content ◽  
Ammonia Nitrogen ◽  
Matter Content ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Elephant Grass ◽  
Linear Effect

SUMMARY The experiment was developed to evaluate the effects of wilting and inclusion of cassava meal level on nutritive value, fermentative characteristics and losses of elephant grass silage. The 4 x 2 factorial scheme was adopted, with four repetitions, in a completely randomized design. The factors evaluated were wilting (without and with) and cassava meal level (0; 7.5; 15 and 22.5% of natural matter, DN). PVC silos with 50 cm height and 10 cm diameter were used for silages production, which were opened after 60 days. The wilting and cassava meal addition had increasing linear effect on DM content. Silages with cassava meal showed reduction of total nitrogen, ether extract, acid detergent insoluble nitrogen, neutral detergent fiber ash and protein free, acid detergent fiber, celluloses, hemicelluloses and lignin contents and increase of organic matter content, DM in vitro digestibility and total digestible nutrients. Relative to variables inherent to silages’ fermentative characteristics, pH presented quadratic behavior. Concerning to ammonia nitrogen, it was observed quadratic and decreasing linear behavior for silages without and with wilting, respectively. Wilting and cassava meal inclusion provided smaller effluent losses.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals Marginal and Internal Fit of Ceramic Prostheses Fabricated from Different Chairside CAD/CAM Systems: An In Vitro Study

2021 ◽  
Vol 11 (2) ◽  
pp. 857
Author(s):  
Keunbada Son ◽  
Kyu-Bok Lee
Keyword(s):  
Computer Aided Design ◽  
In Vitro Study ◽  
Vitro Study ◽  
Marginal Gap ◽  
Significant Difference ◽  
Cad Cam ◽  
Ceramic Crowns ◽  
Internal Fit ◽  
Cam Systems

The purpose of this in vitro study was to evaluate marginal and internal fits of ceramic crowns fabricated with chairside computer-aided design and manufacturing (CAD/CAM) systems. An experimental model based on ISO 12836:2015 was digitally scanned with different intraoral scanners (Omnicam (CEREC), EZIS PO (DDS), and CS3500 (Carestream)). Ceramic crowns were fabricated using the CAD/CAM process recommended by each system (CEREC, EZIS, and Carestream systems; N = 15). The 3-dimensional (3D) marginal and internal fit of each ceramic crown was measured using a 3D inspection software (Geomagic control X). Differences among the systems and various measurements were evaluated using the Kruskal–Wallis test. Statistically significant differences were validated using pairwise comparisons (α = 0.05). Occlusal gaps in the CEREC, EZIS, and Carestream groups were 113.0, 161.3, and 438.2 µm, respectively (p < 0.001). The axial gaps were 83.4, 78.0, and 107.9 µm, respectively. The marginal gaps were 77.8, 99.3, and 60.6 µm, respectively, and the whole gaps were 85.9, 107.3, and 214.0 µm, respectively. Significant differences were observed with the EZIS system compared with the other two systems in terms of the marginal gap sizes. The CEREC system showed no significant differences among the four measured regions. However, the EZIS and Carestream systems did show a statistically significant difference (p < 0.05). All three systems were judged to be capable of fabricating clinically acceptable prostheses, because the marginal gap, which is the most important factor in the marginal fit of prostheses, was recorded to be below 100 µm in all three systems.

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

A novel intracranial exchange guidewire improves the navigation of various endovascular devices: An in vitro study of challenging situations

2021 ◽  
pp. 159101992110573
Author(s):  
Naoki Kaneko ◽  
Ariel Takayanagi ◽  
Hamidreza Saber ◽  
Lea Guo ◽  
Satoshi Tateshima
Keyword(s):  
Giant Aneurysm ◽  
In Vitro Study ◽  
Target Vessel ◽  
Success Rates ◽  
Intracranial Stenting ◽  
Aneurysm Neck ◽  
Significant Difference ◽  
Aneurysm Model ◽  
Endovascular Devices

Objective Neuroendovascular procedures rely on successful navigation and stable access to the target vessel. The Stabilizer is a 300 cm long exchange wire with a 0.014 diameter and a soft, flexible stent at the distal end designed to assist with navigation and device delivery. This study aims to assess the efficacy of the Stabilizer for navigation in a variety of challenging environments. Methods The efficacy of the Stabilizer was evaluated using three challenging vascular models: a giant aneurysm model, a severe tortuosity model, and an M1 stenosis model. The Stabilizer was compared with a conventional wire during navigation in each model. Results In the giant aneurysm model, there was no significant difference of success during straightening of a looped wire and significantly higher success rates when advancing an intermediate catheter with the Stabilizer beyond the aneurysm neck compared to a conventional guidewire. The Stabilizer also significantly increased success rates when advancing an intermediate catheter through a model with severe tortuosity compared to a conventional guidewire, as well as exchange maneuver for intracranial stenting in a stenosis model compared to an exchange wire. Conclusions In our experimental model, the Stabilizer significantly improved navigation and device delivery in a variety of challenging settings compared to conventional wires.

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals Ruminal parameters and ruminal degradability of feedlot sheep fed safflower grains

2018 ◽  
Vol 19 (3) ◽  
pp. 324-335
Author(s):  
Jefferson Leonardo Rocha Alves ◽  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Antônio Campanha Martinez ◽  
Aguinaldo Yoshio Nakamura ◽  
Jefferson Rodrigues Gandra ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Asymptotic Model ◽  
Ruminal Fermentation ◽  
Mean Values ◽  
Ruminal Ph ◽  
Significant Difference ◽  
The Times

SUMMARY This study aimed to evaluate the rumen degradability of dry matter (DM), disappearance of neutral detergent fiber (NDF) of Tifton 85 hay, and fermentation parameters (pH and ruminal ammonia nitrogen) of sheep fed diets with inclusion of safflower grains (0, 7.50 and 15%). Three mixed breed lambs with average body weight of 35.50± 1.50 kg were used. The rumen degradability of dry matter and NDF of the Tifton 85 haywas analyzedby the disappearance of DM and NDF, and the kinetic parameters adjusted according to the first order asymptotic model. The samples were incubated in the rumen in descending order (96, 48, 24, 12, 6, 3, 0 hours). The determination of ruminal pH and ruminal ammonia nitrogen (RAN) was performed before feeding and 2, 4, 6 and 8 hours after feeding. Data of ruminal fermentation were tested by analysis of variance with repeated measures in time, at 5% probability. Ruminal pH values were not statistically different. When evaluating the RAN, there was a significant difference between the times and treatments for the diet with 15% safflower. The diets presented low effective degradability of DM and NDF with mean values of 18.02% and 47.40%, respectively. The safflower grain can be used up to 7.50% in the diets without altering the degradability and ruminal fermentation.

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