scholarly journals Community of natural lactic acid bacteria and silage fermentation of corn stover and sugarcane tops in Africa

2020 ◽  
Vol 33 (8) ◽  
pp. 1252-1264 ◽  
Author(s):  
Yimin Cai ◽  
Zhumei Du ◽  
Seishi Yamasaki ◽  
Damiao Nguluve ◽  
Benedito Tinga ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Corn Stover ◽  
Animal Feed ◽  
Dry Season ◽  
Neutral Detergent Fiber ◽  
Agricultural Field ◽  
Fresh Matter ◽  
Silage Fermentation ◽  
Dominant Bacteria

Objective: To effectively utilize crop by-product resources to address the shortage of animal feed during the dry season in Africa, the community of natural lactic acid bacteria (LAB) of corn stover and sugarcane tops and fermentation characteristics of silage were studied in Mozambique.Methods: Corn stover and sugarcane tops were obtained from agricultural field in Mozambique. Silage was prepared with LAB inoculant and cellulase enzyme and their fermentation quality and microbial population were analyzed.Results: Aerobic bacteria were the dominant population with 10<sup>7</sup> colony-forming unit/g of fresh matter in both crops prior to ensiling, while 10<sup>4</sup> to 10<sup>7</sup> LAB became the dominant bacteria during ensiling. <i>Lactobacillus plantarum</i> was more than 76.30% of total isolates which dominated silage fermentation in the LAB-treated sugarcane top silages or all corn stover silages. Fresh corn stover and sugarcane tops contain 65.05% to 76.10% neutral detergent fiber (NDF) and 6.52% to 6.77% crude protein (CP) on a dry matter basis, and these nutrients did not change greatly during ensiling. Corn stover exhibits higher LAB counts and watersoluble carbohydrates content than sugarcane top, which are naturally suited for ensiling. Meanwhile, sugarcane tops require LAB or cellulase additives for high quality of silage making.Conclusion: This study confirms that both crop by-products contain certain nutrients of CP and NDF that could be well-preserved in silage, and that they are potential roughage resources that could cover livestock feed shortages during the dry season in Africa.

scholarly journals Effects of Phenyllactic Acid, Lactic Acid Bacteria, and Their Mixture on Fermentation Characteristics and Microbial Community Composition of Timothy Silage

2021 ◽  
Vol 12 ◽  
Author(s):  
Ping Li ◽  
Yongxiang Lu ◽  
Man Zhao ◽  
Liangyin Chen ◽  
Changbin Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Microbial Community ◽  
Lactic Acid Bacteria ◽  
Community Composition ◽  
Crude Protein ◽  
Microbial Community Composition ◽  
Phenyllactic Acid ◽  
Fresh Matter ◽  
Silage Fermentation ◽  
Protein Preservation

This study investigated the effects of phenyllactic acid (PL), lactic acid bacteria (LAB), and their mixture on fermentation characteristics and microbial community composition of timothy silage. Timothy silages were treated without (CK) or with PL [10 mg/kg fresh matter (FM) basis], LAB inoculant (IN; a mixture of Lactobacillus plantarum and L.buchneri, 105 cfu/g FM), and their mixture (PI) and stored at ambient temperature (5°C∼15°C) in a dark room for 60 days. Compared with CK, all treated silages showed lower (P &lt; 0.05) levels of butyric acid and ammonia-N. Treatment with PL enhanced (P &lt; 0.05) the crude protein preservation of silage by favoring the growth of L. curvatus and Saccharomyces cerevisiae and inhibition of lactic acid–assimilating yeast belonging to Issatchenkia during ensiling. In particular, treatment with PL advanced (P &lt; 0.05) the productions of lactic acid and volatile fatty acid in IN-treated silage. Therefore, PL used as a new additive exhibited potential for improving silage fermentation when it is combined with LAB IN during ensiling.

scholarly journals Effects of Ensiling Oxytropis glabra with Whole-Plant Corn at Different Proportions on Fermentation Quality, Alkaloid Swainsonine Content, and Lactic Acid Bacteria Populations

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1733
Author(s):  
Ya Tao ◽  
Dongze Niu ◽  
Feng Li ◽  
Sasa Zuo ◽  
Qizhong Sun ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Removal Rate ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrate ◽  
Residual Water ◽  
Fresh Matter ◽  
Whole Plant ◽  
Water Soluble Carbohydrate

Oxytropis glabra (OG) is a leguminous forage that is potentially valuable for solving the shortage of feed for livestock production, while, in large quantities, it may be toxic because of its swainsonine (SW) content. In this study, OG was ensiled with whole-plant corn (Zea mays L.) at 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, and 0:10 ratios on a fresh matter basis, and, after 60 d of ensiling, the chemical composition, fermentation characteristic, SW removal rate, lactic acid bacteria (LAB) populations, and their capabilities for SW removal were analyzed. As the proportion of corn in the silage increased, the pH, as well as the propionic acid, ammonia-N, dry matter, crude protein, and SW contents, decreased linearly, while the lactic acid, neutral detergent fiber, and residual water-soluble carbohydrate contents increased linearly. Lactobacillus plantarum was the most common microorganism present in all mixture silages. Lactobacillus amylovorus and Lactobacillusbrevis were prevalent at lower ratios of corn to OG. Meanwhile, the LAB strains belong to L. amylovorus and L. plantarum had a higher SW removal rate. Our results suggested that ensiling OG with whole-plant corn improves fermentation and decreases SW content, and that 5:5 is the optimal ratio, so this type of mixed silage could make OG useable for ruminant production.

scholarly journals The Effects of Lactic Acid Bacteria and Enzyme Mixture Inoculants on Silage Fermentation Characteristics and Feed Values of Silage Prepared from Alfalfa Harvested at Different Maturities

2021 ◽  
Vol 9 (6) ◽  
pp. 1062-1069
Author(s):  
Berrin Okuyucu ◽  
Selma Büyükkılıç Beyzi ◽  
Mehmet Levent Özdüven
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Metabolic Energy ◽  
Plastic Bags ◽  
Silage Fermentation ◽  
Feed Value ◽  
High Doses

This study was carried out to determine the effects of lactic acid bacteria+ enzyme (LAB+E) inoculants on the fermentation characteristics and feed values of silages prepared from alfalfa harvested at three maturity stages. Alfalfa was harvested at the early, middle and late flowering stages. Sil-All (Alltech, UK) were used as LAB+E inoculants. Inoculants were applied to the silages at the rates of 1×105, 5×105 and 1×106 cfu/g levels in 1 liter capacity plastic bags. The bags were stored at 20±2°C under the laboratory conditions. Three bags from each group were sampled for chemical and microbiological analyses on the 45th day after ensiling. The results showed that LAB+E inoculants reduced pH values and ammonia-nitrogen content, whereas increased lactic acid contents and lactobacillus count of alfalfa silages. High doses LAB+E inoculant decreased neutral detergent fiber and acid detergent fiber content, increased in vitro organic matter digestibility and metabolic energy of alfalfa silages. It has been demonstrated that the most effective application dose of LAB+E inoculant to improve fermentation and feed value of alfalfa silage was 1×106 cfu/g, but 1x105 and 5×105 cfu/g level can also be considered as effective dose.

scholarly journals PENGARUH LAMA ENSILASE TERHADAP KUALITAS FRAKSI SERAT KASAR SILASE LIMBAH PUCUK TEBU (Saccharum officinarum) YANG DIINOKULASI DENGAN BAKTERI ASAM LAKTAT TERSELEKSI

2016 ◽  
Vol 13 (1) ◽  
pp. 85
Author(s):  
A. Fariani ◽  
S. Akhadiarto
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Animal Feed ◽  
Saccharum Officinarum ◽  
Fiber Fraction ◽  
Silage Fermentation ◽  
Randomized Design ◽  
Fermentation Quality ◽  
Completely Randomized Design

Tujuan penelitian ini adalah untuk mengetahui pengaruh lama ensilase terhadap kualitas fraksi serat kasar silase limbah pucuk tebu (Saccharum officinarum) yang diinokulasi dengan bakteri asam laktat yang dipilih. Penelitian ini dilakukan dalam dua Tahap. Tahap pertama adalah pengayakan dan seleksi bakteri asam laktat dari pucuk tebu dan tahap kedua adalah pembuatan silase pucuk tebu dengan bakteri asam laktat hasil penelitian tahap pertama. Penelitian dilakukan di laboratorium Nutrisi dan Makanan Ternak Fakultas Pertanian Universitas Sriwijaya, Palembang. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 4 ulangan : P1 (lama fermentasi 7 hari), P2 (lama fermentasi 14 hari), P3 (lama fermentasi 21 hari), dan P4 (lama fermentasi 28 hari). Parameter yang diamati adalah NDF, ADF, selulosa, lignin, pHsilase dan populasi bakteri asam laktat. Hasil penelitian menunjukkan, perlakuan yang memberikan pengaruh signifikan pada NDF (P1 66.90%, P2 70.79%, P3 70.83% and P4 69.26%) dan ADF (P1 62.78%, P2 63.41%, P3 63.58% and P4 66.94%, sedangkan untuk selulosa, lignin, dan pH silase adalah non signifikan. Disimpulkan bahwa inokulasi bakteri asam laktat terseleksi dapat meningkatkan kualitas fermentasi silase pucuk tebu dan waktu ensilase dapat dipercepat dari 21 hari menjadi 7 hari. kata kunci : lama ensilase, kualitas fraksi serat kasar, inokulasi, bakteri asam laktat. AbstractThe objective of this research was to know the effect of ensilage time on fiber fraction quality of sugarcane top (Saccharum officinarum) inoculated with selected lactic acid bacteria. This research was conducted in two stage. First stage was lactic acid bacteria isolated from sugarcane top and second sugarcane top ensilage with selected lacticacid bacteria. There were held on Animal feed and Nutritive Laboratory Faculty of Agriculture, Sriwijaya University. This research used Completely Randomized Design with 4 treatments and 4 replications: P1 (7 days ensilage), P2 (14 days ensilage), P3 (21 days ensilage), P4 (28 days ensilage). Observed parameters were NDF, ADF,cellulose, lignin, pH silage and population lactic acid bacteria. The result showed that treatment gave significant effect on NDF (P1 66.90%, P2 70.79%, P3 70.83% and P4 69.26%) and ADF (P1 62.78%, P2 63.41%, P3 63.58% and P4 66.94%, however cellulose, lignin and pH silage were non significant. In conclution, selected lactic acidbacteria could improved silage fermentation quality of sugarcane top and ensilage time were improved from 21 days to 7 days. key Words: Ensilage time, fiber fraction quality, inoculant, lactic acid bactery

Evaluation of lactic acid bacteria isolated from alfalfa for silage fermentation

2018 ◽  
Vol 64 (3) ◽  
pp. 190-198 ◽  
Author(s):  
Dongxia Li ◽  
Yanping Wang ◽  
Yingchao Zhang ◽  
Yanli Lin ◽  
Fuyu Yang
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Silage Fermentation

LACTIC ACID BACTERIA IN FOOD AND HEALTH: A REVIEW WITH SPECIAL REFERENCE TO ENTEROPATHOGENIC ESCHERICHIA COLI AS WELL AS CERTAIN ENTERIC DISEASES AND THEIR TREATMENT WITH ANTIBIOTICS AND LACTOBACILLI1

1972 ◽  
Vol 35 (12) ◽  
pp. 691-702 ◽  
Author(s):  
W. E. Sannine ◽  
K. S. Muralidhara ◽  
P. R. Elliker ◽  
D. C. England
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Animal Feed ◽  
Large Population ◽  
Intestinal Bacteria ◽  
Transfer Factors ◽  
Enteric Diseases ◽  
Human Vagina ◽  
Vaginal Disease

Recent literature concerning enteropathogenesis and drug resistance transfer factors in Escherichia coli are summarized as well as related papers concerning the use of antibiotics in animal feed. E. coli infection in swine (colibacillosis) also is considered, especially citations indicating the similarity between the disease in man and animals. The role of intestinal bacteria in human health is reviewed, emphasizing the importance of (a) a maintained balance of organisms in the adult, (b) breast feeding in infants to establish a large population of bifidobacteria and (c) the presence of Lactobacillus organisms to maintain healthful conditions in the human vagina. The use of Lactobacillus organisms in intestinal and vaginal disease therapy is reviewed as well as the important ecological role that lactic acid bacteria play in the natural scheme where man is concerned.

The voluntary intake and digestibility by sheep of grass silages treated with different silage inoculants

1991 ◽  
Vol 1991 ◽  
pp. 82-82
Author(s):  
J A Rooke ◽  
F Kafilzadeh
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Live Weight ◽  
Random Order ◽  
Animal Performance ◽  
Silage Fermentation ◽  
Silage Inoculants ◽  
Silage Additives ◽  
Forage Harvester ◽  
Silage Inoculant

The selection of lactic acid bacteria for use as silage additives is normally based upon their ability to dominate the silage fermentation and not upon benefits in animal performance. The object of this study was to investigate whether two lactic acid bacteria selected for fermentation characteristics would support the same animal performance as an established silage inoculant.On 5 June 1989, first cut, predominantly perennial ryegrass (Lolium perenne) was ensiled direct with no wilting in plastolene silos of 2 tonne capacity. The herbage was harvested with a precision chop forage harvester and the following additive treatments were applied: None, control (C); Formic acid (Add-F, BP Nutrition, 850g/kg; 3 litres/tonne), (F); inoculant E. (Ecosyl, ICI Pic, 106Lactobacillus plantarum /g): inoculant A, (Pediococcus sp, 10 /g); inoculant B (L. plantarum 106 /g). The silages were fed to 6 wether sheep (Suffolk x Halfbred), initial live-weight, 40.1 kg (s.d. 2.22kg). Because the silages were unstable aerobically and restricted quantities of each silage were available, all sheep were fed the silages in the same (random) order.

Effects of adding a Lactobacillus Plantarum inoculant to grass ensiled at different dry matter concentrations and offered to beef cattle

1994 ◽  
Vol 1994 ◽  
pp. 115-115 ◽  
Author(s):  
P. O'Kiely
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Beef Cattle ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Nutritive Value ◽  
Silage Fermentation ◽  
Different Levels

Silage fermentation is progressively restricted as the extent of pre-wilting increases (O'Kiely et_al., 1988). The magnitude of the improvement in silage nutritive value in response to a lactic acid bacteria inoculant could be related to the extent of the fermentation in the untreated silage. The objective of this experiment was to determine if the response in silage nutritive value to a Lactobacillus plantarum inoculant was similar at different levels of dry matter (DM) concentration.

Epiphytic lactic acid bacteria succession during the pre-ensiling and ensiling periods of alfalfa and maize

1992 ◽  
Vol 1992 ◽  
pp. 155-155
Author(s):  
Chunjian Lin ◽  
K. K. Bolsen ◽  
B. E. Brent ◽  
D.Y.C. Fung ◽  
W. R. Aimutis
Keyword(s):  
Zea Mays ◽  
Lactic Acid ◽  
North America ◽  
Lactic Acid Bacteria ◽  
Limited Information ◽  
Maize Hybrids ◽  
Silage Fermentation ◽  
Whole Plant ◽  
Three Stages ◽  
Second Year

Epiphytic LAB, e.g., lactobacilli, lactococci, enterococci, pediococci, streptococci, and leuconostocs, play a major role in silage fermentation. Their numbers and populations have become a concern in predicting the adequacy of silage fermentation and in determining whether or not to apply a bacterial inoculant (Bolsen et al, 1989). Epiphytic LAB counts are usually low and variable on silage crops (Lin et al, 1991), and increases in the LAB counts usually occur coincident to the chopping process. Only limited information is available concerning the succession of epiphytic LAB species during the ensiling period of alfalfa (Medicago sativaL.) and maize (Zea mays L.), the two major silage crops in North America. The present studies investigated the epiphytic LAB succession during the pre-ensiling and ensiling periods for two cuttings of alfalfa, each harvested at three stages of maturity, and three whole-plant maize hybrids.A second-year stand of alfalfa was harvested at the 2nd and 4th cuttings and at the late-bud, 10% bloom, and 50% bloom stages of maturity within each cutting in 1989. Following mowing, the alfalfa was wilted in the windrow for 5 to 6 hours prior to precision chopping.

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