Evaluation of lactic acid bacteria isolated from alfalfa for silage fermentation

2018 ◽  
Vol 64 (3) ◽  
pp. 190-198 ◽  
Author(s):  
Dongxia Li ◽  
Yanping Wang ◽  
Yingchao Zhang ◽  
Yanli Lin ◽  
Fuyu Yang
Author(s):  
J A Rooke ◽  
F Kafilzadeh

The selection of lactic acid bacteria for use as silage additives is normally based upon their ability to dominate the silage fermentation and not upon benefits in animal performance. The object of this study was to investigate whether two lactic acid bacteria selected for fermentation characteristics would support the same animal performance as an established silage inoculant.On 5 June 1989, first cut, predominantly perennial ryegrass (Lolium perenne) was ensiled direct with no wilting in plastolene silos of 2 tonne capacity. The herbage was harvested with a precision chop forage harvester and the following additive treatments were applied: None, control (C); Formic acid (Add-F, BP Nutrition, 850g/kg; 3 litres/tonne), (F); inoculant E. (Ecosyl, ICI Pic, 106Lactobacillus plantarum /g): inoculant A, (Pediococcus sp, 10 /g); inoculant B (L. plantarum 106 /g). The silages were fed to 6 wether sheep (Suffolk x Halfbred), initial live-weight, 40.1 kg (s.d. 2.22kg). Because the silages were unstable aerobically and restricted quantities of each silage were available, all sheep were fed the silages in the same (random) order.


Author(s):  
P. O'Kiely

Silage fermentation is progressively restricted as the extent of pre-wilting increases (O'Kiely et_al., 1988). The magnitude of the improvement in silage nutritive value in response to a lactic acid bacteria inoculant could be related to the extent of the fermentation in the untreated silage. The objective of this experiment was to determine if the response in silage nutritive value to a Lactobacillus plantarum inoculant was similar at different levels of dry matter (DM) concentration.


Author(s):  
Chunjian Lin ◽  
K. K. Bolsen ◽  
B. E. Brent ◽  
D.Y.C. Fung ◽  
W. R. Aimutis

Epiphytic LAB, e.g., lactobacilli, lactococci, enterococci, pediococci, streptococci, and leuconostocs, play a major role in silage fermentation. Their numbers and populations have become a concern in predicting the adequacy of silage fermentation and in determining whether or not to apply a bacterial inoculant (Bolsen et al, 1989). Epiphytic LAB counts are usually low and variable on silage crops (Lin et al, 1991), and increases in the LAB counts usually occur coincident to the chopping process. Only limited information is available concerning the succession of epiphytic LAB species during the ensiling period of alfalfa (Medicago sativaL.) and maize (Zea mays L.), the two major silage crops in North America. The present studies investigated the epiphytic LAB succession during the pre-ensiling and ensiling periods for two cuttings of alfalfa, each harvested at three stages of maturity, and three whole-plant maize hybrids.A second-year stand of alfalfa was harvested at the 2nd and 4th cuttings and at the late-bud, 10% bloom, and 50% bloom stages of maturity within each cutting in 1989. Following mowing, the alfalfa was wilted in the windrow for 5 to 6 hours prior to precision chopping.


2016 ◽  
Vol 51 (5) ◽  
pp. 316-325 ◽  
Author(s):  
Yan-Su Wang ◽  
Tian-Hao Wu ◽  
Yao Yang ◽  
Cen-Ling Zhu ◽  
Cheng-Long Ding ◽  
...  

1994 ◽  
Vol 58 (8) ◽  
pp. 1412-1415 ◽  
Author(s):  
Osamu Tanaka ◽  
Hidetoshi Kimura ◽  
Eiji Takahashi ◽  
Seiya Ogata ◽  
Sadahiro Ohmomo

2021 ◽  
Author(s):  
Qiming Cheng ◽  
Liangyin Chen ◽  
Yulian Chen ◽  
Ping Li ◽  
Chao Chen

Abstract Background: Lactic acid bacteria have been proposed for the control of undesirable fermentation and subsequently aerobic deterioration due to their ability to produce antimicrobial metabolites in silage mass. To investigate the effect of specific LAB on silage fermentation characteristics and bacterial community composition of oat in cold region, silages were treated without additives (CK) or with three LAB strains (LB, Lactobacillus buchneri; nLP, low temperature tolerant Lactobacillus planrtarum; pLP, phenyllactic acid-producing Lactobacillus plantarum), and then stored at ambient temperature (< 20 ℃) for 30, 60 and 90 days. Results: Compared with CK, inoculation of LAB decreased final pH value, butyric acid content, ammonia-N of total N and dry matter loss of silage. Treatments with nLP and pLP increased (P < 0.05) lactic acid content, whereas LB increased (P < 0.05) acetic acid content of silage. Lactobacillus and Leuconstoc dominated in the silages with relative abundance of 68.29~96.63%. A prolonged storage period enhanced growth of Leuconstoc in pLP treated silage. In addition, pLP increased (P < 0.05) aerobic stability of silage as compared with nLP. Conclusions: In conclusion, inoculation of LAB improved silage fermentation and/or delayed aerobic deterioration by shifting bacterial community composition during ensiling. Phenyllactic acid-producing Lactobacillus plantarum as an inoculant exhibited potential for high quality silage production.


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