scholarly journals Effect of single nucleotide polymorphisms on intramuscular fat content in Hungarian Simmental cattle

2018 ◽  
Vol 31 (9) ◽  
pp. 1415-1419 ◽  
Author(s):  
István Anton ◽  
Balázs Húth ◽  
Imre Füller ◽  
László Rózsa ◽  
Gabriella Holló ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Simmental Cattle ◽  
Intramuscular Fat Content

scholarly journals Association of Twelve Candidate Gene Polymorphisms with the Intramuscular Fat Content and Average Backfat Thickness of Chinese Suhuai Pigs

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 858 ◽  
Author(s):  
Wang ◽  
Li ◽  
Zhou ◽  
Gao ◽  
Liu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Gene Polymorphisms ◽  
Genetic Correlations ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Backfat Thickness ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Coefficients Of Variation ◽  
Intramuscular Fat Content

The present study aimed to identify the molecular markers for genes that influence intramuscular fat content (IFC), but not average backfat thickness (ABT). A total of 330 Suhuai pigs were slaughtered, and measurements of IFC and ABT were obtained. Phenotypic and genetic correlations between IFC and ABT were calculated. Thirteen single nucleotide polymorphisms (SNPs) among 12 candidate genes for IFC were analyzed, including FABP3, LIPE, IGF1, IGF2, LEP, LEPR, MC4R, PHKG1, RETN, RYR1, SCD, and UBE3C. Associations of the evaluated SNPs with IFCIFC and ABT were performed. Our results showed that the means of IFC and ABT were 1.99 ± 0.03 % and 26.68 ± 0.28 mm, respectively. The coefficients of variation (CVs) of IFC and ABT were 31.21% and 19.36%, respectively. The phenotypic and genetic correlations between IFC and ABT were moderate. Only the FABP3 (rs1110770079) was associated with IFC (p < 0.05) but not with ABT. Besides, there was a tendency for associations of RYR1 (rs344435545) and SCD (rs80912566) with IFC (p < 0.1). Our results indicated that the FABP3 (rs1110770079) SNP could be used as a marker to improve IFC without changing ABT in the Suhuai pig breeding system.

Single-nucleotide polymorphisms for matrix metalloprotease-1 can affect perimysial strength and intramuscular fat content but not growth rate of cattle

2020 ◽  
Vol 60 (16) ◽  
pp. 1869
Author(s):  
S. Christensen ◽  
C. Monteavaro ◽  
P. P. Purslow
Keyword(s):  
Growth Rate ◽  
Connective Tissue ◽  
Growth Curves ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Matrix Metalloprotease ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Intramuscular Fat Content

Context Single-nucleotide polymorphisms (SNPs) in the gene coding for matrix metalloprotease-1 (MMP-1) are known to affect the level of intramuscular fat found in cattle. As well as a signalling molecule affecting adipogenesis, MMP-1 is a major collagenase involved in the turnover of connective tissue. Aims The aim of the work was to assess whether SNPs in the gene for MMP-1 may affect the mechanical properties of intramuscular connective tissue, and therefore meat texture. Methods Allelic frequencies of three SNPs for MMP-1 were determined in a group of black Aberdeen Angus cattle whose growth characteristics had been traced for 450 days before slaughter. Associations between the alleles of each of the three SNPs and growth rate, killing out percentage, half-carcass weight, intramuscular fat content, cooking loss, strength of perimysium in cooked M. semitendinosus and Warner–Bratzler peak force of cooked M. longissimus dorsi were studied. Key results None of the SNPs studied had any effect on growth curves, and only one SNP (ss77831914) showed differences in half-carcass weight between alleles. Carcass yield and killing out percentage showed a small difference between alleles of ss7783924. No effects were found on the Warner–Bratzler peak force of M. longissimus dorsi cooked to 70°C. Two SNPs (ss77831914 and ss77831924) showed significant differences between alleles in the raw strength of perimysium in M. semitendinosus and the amount of intramuscular fat. Conclusions Commonly occurring SNPs of the major collagenase MMP-1 can affect the strength of intramuscular connective tissue as well as intramuscular fat content. Although these differences in connective tissue strength do not influence Warner–Bratzler measures of toughness at a cooking temperature of 70°C, they may contribute to differences in toughness in low-temperature, long-time cooking. Implications Because none of the SNPs had effects on the growth curves of the cattle studied, selection of animals with the relevant alleles of SNPs ss77831914 ss77831924 could be used to produce more tender meat without affecting carcass yield.

scholarly journals Effect of single-nucleotide polymorphisms on the breeding value of fertility and breeding value of beef in Hungarian Simmental cattle

2018 ◽  
Vol 66 (2) ◽  
pp. 215-225 ◽  
Author(s):  
István Anton ◽  
Balázs Húth ◽  
Imre Füller ◽  
György Gábor ◽  
Gabriella Holló ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Mixed Model ◽  
Breeding Value ◽  
Nucleotide Polymorphisms ◽  
Molecular Tools ◽  
Single Nucleotide ◽  
Chip Data ◽  
Minor Alleles ◽  
Simmental Cattle ◽  
Data Screening

The objective of this study was to estimate the effect of single-nucleotide polymorphisms (SNPs) on the breeding value of fertility (BVF) and the breeding value of beef (BVB) in Hungarian Simmental cattle. Genotypes were determined on a high-density Illumina Bovine DNA Chip. Data screening and data identification were performed by multi-locus mixed-model. Statistical analyses were carried out to find associations between individual genotypes and the investigated quality values. Three loci showed considerable association with BVF (–log10 P = 9.5, 9.9 and 14.5, respectively) on chromosomes 9, 28 and 29, respectively. The frequencies of their minor alleles (MAF) were 0.375, 0.355 and 0.354, respectively. Two loci showed association with BVB (–log10 P = 25.3 and 22.7) on chromosomes 2 and 11, respectively (their MAF were 0.438 and 0.229). The abovementioned loci provide a straightforward possibility to assist selection by molecular tools.

Identification of 14 new single nucleotide polymorphisms in the bovineSLC27A1gene and evaluation of their association with milk fat content

2008 ◽  
Vol 75 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Laura Ordovas ◽  
Pilar Zaragoza ◽  
Juan Altarriba ◽  
Clementina Rodellar
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Milk Fat ◽  
Energy Homeostasis ◽  
Integral Membrane Protein ◽  
Fat Content ◽  
Sequencing Analysis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Isolation And Characterization ◽  
Holstein Friesian

The solute carrier family 27 member 1 (SLC27A1) is an integral membrane protein involved in the transport of long-chain fatty acids across the plasma membrane. This protein has been implicated in diet-induced obesity and is thought to be important in the control of energy homeostasis. In previous reports, our group described the isolation and characterization of the bovineSLC27A1gene. The bovine gene is organized in 13 exons spanning over more than 40 kb of genomic DNA and maps in BTA 7 where several quantitative trait loci for fat related traits have been described. Because of its key role in lipid metabolism and its genomic localization, in the present work the search for variability in the bovineSLC27A1gene was carried out with the aim of evaluating its potential association with milk fat content in dairy cattle. By sequencing analysis of all exons and flanking regions 14 new single nucleotide polymorphisms (SNPs) were identified: 1 in the promoter, 7 in introns and 6 in exons. Allele frequencies of all the SNPs were calculated by minisequencing analysis in two groups of Holstein-Friesian animals with highest and lowest milk-fat content estimated breeding values as well as in animals of two Spanish cattle breeds, Asturiana de los Valles and Menorquina. In the conditions assayed, no significant differences between Holstein-Friesian groups were found for any of the SNPs, suggesting that theSLC27A1gene may have a poor or null effect on milk fat content. In Asturiana and Menorquina breeds all the positions were polymorphic with the exception of SNPs 1 and 8 in which C allele was fixed in both of them.

Lack of an association between single nucleotide polymorphisms in the bovine leptin gene and intramuscular fat in Bos taurus cattle

Meat Science ◽  
2009 ◽  
Vol 81 (4) ◽  
pp. 731-737 ◽  
Author(s):  
L. Pannier ◽  
T. Sweeney ◽  
R.M. Hamill ◽  
F. Ipek ◽  
P.C. Stapleton ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Bos Taurus ◽  
Intramuscular Fat ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Effect of thyroglobulin gene polymorphism on the intramuscular fat content in cattle examined by x-ray computed tomography and Soxhlet methods

2013 ◽  
Vol 56 (1) ◽  
pp. 593-596
Author(s):  
I. Anton ◽  
A. Zsolnai ◽  
I. Holló ◽  
I. Repa ◽  
G. Holló
Keyword(s):  
Positive Association ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Quality Trait ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Longissimus Dorsi Muscle ◽  
X Ray Computed ◽  
Thyroglobulin Gene ◽  
Intramuscular Fat Content

Abstract. Intramuscular fat content or marbling represents a valuable beef quality trait and is an important determinant of palatability due to its contribution to juiciness and flavour. Meat which has little marbling may be dry and flavourless. Higher levels of intramuscular fat have been associated with increased tenderness, juiciness and flavour of beef (Thompson 2004). Whereas this feature is highly appreciated in some markets (by example Australia, China, Korea, Japan) breeders usually try to increase intramuscular fat deposition in cattle by nutrition. However, genetic factors have been shown to affect marbling in cattle. Thyroglobulin (TG) is a glycoproteic hormone synthesised in the thyroid gland and is the precursor of triiodothyronine (T3) and thyroxine (T4) hormones which influence lipid metabolism. T3 and T4 hormones have been associated with marbling in Wagyu cattle (Mears et al. 2001). The effect of a C/T single nucleotide polymorphism in the 5'-untranslated region of TG gene has been concluded to affect intramuscular fat content in cattle (Barendse 1999). High probability of positive association between the T allele of bovine TG gene and marbling was observed by Wood et al. (2006). Thyroglobulin mainly affects the fat content of longissimus dorsi muscle (LD) (Thaller et al. 2003).

scholarly journals Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 150
Author(s):  
Isaac Hyeladi Malgwi ◽  
Veronika Halas ◽  
Petra Grünvald ◽  
Stefano Schiavon ◽  
Ildikó Jócsák
Keyword(s):  
Molecular Mechanisms ◽  
Fat Metabolism ◽  
Gene Interaction ◽  
Acid Synthesis ◽  
Intramuscular Fat ◽  
Genomic Research ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Nutritional Science ◽  
Intramuscular Fat Content

Fat metabolism and intramuscular fat (IMF) are qualitative traits in pigs whose development are influenced by several genes and metabolic pathways. Nutrigenetics and nutrigenomics offer prospects in estimating nutrients required by a pig. Application of these emerging fields in nutritional science provides an opportunity for matching nutrients based on the genetic make-up of the pig for trait improvements. Today, integration of high throughput “omics” technologies into nutritional genomic research has revealed many quantitative trait loci (QTLs) and single nucleotide polymorphisms (SNPs) for the mutation(s) of key genes directly or indirectly involved in fat metabolism and IMF deposition in pigs. Nutrient–gene interaction and the underlying molecular mechanisms involved in fatty acid synthesis and marbling in pigs is difficult to unravel. While existing knowledge on QTLs and SNPs of genes related to fat metabolism and IMF development is yet to be harmonized, the scientific explanations behind the nature of the existing correlation between the nutrients, the genes and the environment remain unclear, being inconclusive or lacking precision. This paper aimed to: (1) discuss nutrigenetics, nutrigenomics and epigenetic mechanisms controlling fat metabolism and IMF accretion in pigs; (2) highlight the potentials of these concepts in pig nutritional programming and research.

scholarly journals Determination of the effect of functional single-nucleotide polymorphisms associated with glycerolipid synthesis on intramuscular fat deposition in Korean cattle steer

2021 ◽  
Vol 64 (1) ◽  
pp. 27-33
Author(s):  
Hyeongrok Kim ◽  
Dong-Yep Oh ◽  
Yoonseok Lee
Keyword(s):  
Lipid Metabolism ◽  
Single Nucleotide Polymorphisms ◽  
Intramuscular Fat ◽  
Fat Deposition ◽  
Seed Region ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Korean Cattle ◽  
Triacylglycerol Synthesis ◽  
Glycerolipid Synthesis

Abstract. Intramuscular fat deposition in the longissimus dorsi muscle (LM) of Korean cattle steer is regulated by several genes related to lipid metabolism. One of these genes encodes the enzyme bovine glycerol-3-phosphate acyltransferase, mitochondrial (GPAM), which is located on the mitochondrial outer membrane and catalyzes the initial and committed step of glycerolipid synthesis in lipid metabolism of cattle. Previous studies have shown that the 3′-untranslated region (UTR) of the GPAM is quite extended and contains a polyadenylation signal site, erythroid 15-lipoxygenase differentiation control elements (15-LOX-DICEs), and cytoplasmic polyadenylation elements (CPEs) that affect the regulation of triacylglycerol synthesis. Therefore, the aim of this study was to identify single-nucleotide polymorphisms (SNPs) related to the regulation of glycerolipid synthesis in the 3′-UTR of GPAM and to verify the function of SNPs affecting the deposition of intramuscular fat in Korean cattle steer. In the present study, 11 SNPs were discovered in the 3′-UTR of GPAM. Among these SNPs, g.54853A>G, g.55441A>G, and g.55930C>T were significantly associated with marbling score in a Korean cattle steer population and were strongly correlated with each other within the GPAM gene. Furthermore, based on the results predicted by the RNAhybrid program, four putative microRNAs (miRNAs) were identified, and the above SNPs were found to present in the seed region of these miRNAs. These miRNAs have a differential binding affinity for each allele of SNPs g.54853A>G, g.55441A>G, and g.55930C>T. The in vivo evidence of intramuscular fat deposition in the LM tissue showed that these SNPs affected the regulation of intramuscular fat deposition in Korean cattle steer. Thus, the g.54853A>G, g.55441A>G, and g.55930C>T could be considered as causal mutations regulating intramuscular fat deposition in Korean cattle steer.

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