scholarly journals Atmospheric Pressure MALDI-IMS Using p-Nitroaniline as the Matrix at High Spatial Resolution in the Positive and Negative Ion Modes

2014 ◽  
Vol 62 (4) ◽  
pp. 29-36 ◽  
Author(s):  
Shoko MATSUSHITA ◽  
Eiji SUGIYAMA ◽  
Takahiro HAYASAKA ◽  
Noritaka MASAKI ◽  
Mitsutoshi SETOU
Keyword(s):  
Spatial Resolution ◽  
Atmospheric Pressure ◽  
High Spatial Resolution ◽  
Negative Ion ◽  
Maldi Ims ◽  
The Matrix ◽  
Atmospheric Pressure Maldi

High-Spatial-Resolution OH Rotational Temperature Measurements in an Atmospheric-Pressure Flame Using an Indium-Based Resonance Ionization Detector

1995 ◽  
Vol 49 (5) ◽  
pp. 655-659 ◽  
Author(s):  
Giuseppe A. Petrucci ◽  
Denise Imbroisl ◽  
Robert D. Guenard ◽  
Benjamin W. Smith ◽  
Jame D. Winefordner
Keyword(s):  
Temperature Profile ◽  
Spatial Resolution ◽  
Atmospheric Pressure ◽  
High Spatial Resolution ◽  
Rotational Temperature ◽  
Resonance Ionization ◽  
Excitation Spectra ◽  
Excitation Beam ◽  
Flame Region ◽  
Collisional Ionization

The use of a resonance ionization photon detector (RID) is described for the measurement of flame temperatures with a spatial resolution of less than 100 μm. The detector, based on the two-step excitation of indium atoms, with subsequent collisional ionization, was used to record rotational excitation scans of OH in an atmospheric-pressure acetylene/air flame. The OH excitation spectra were recorded by scanning an “excitation” laser in the A2σ+ ← X2II i (1, 0) vibronic band in the wavelength range, 281–288 nm, while simultaneously illuminating the same flame region with the “detection” laser, tuned to the 6 p2 P3/2 → 10 d2. D5/2 excited-state transition of In at 786.44 nm. The excitation and detection laser beams were made orthogonal in the flame, defining the resolution to be limited by the waist of the excitation beam (100 μm), whose diameter was always smaller than the detection laser beam. A temperature profile of the flame is recorded with the use of both the RID approach and a more conventional laser-induced fluorescence (LIF) approach for comparison. A more structured temperature profile is recorded with the RID owing to its high spatial resolution, whereas the LIF method, which is inherently a line-of-sight method, produces a rather featureless temperature distribution across the flame. Anomalously high flame temperatures were recorded at the flame edge with the RID. The cause of these high flame temperatures has not been determined.

High-Spatial-Resolution Machining Utilizing Atmospheric Pressure Plasma: Machining Characteristics of Silicon

2006 ◽  
Vol 45 (10B) ◽  
pp. 8281-8285 ◽  
Author(s):  
Kazuya Yamamura ◽  
Kunihito Kato ◽  
Yasuhisa Sano ◽  
Masafumi Shibahara ◽  
Katsuyoshi Endo ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Atmospheric Pressure ◽  
High Spatial Resolution ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma ◽  
Machining Characteristics

scholarly journals Volatile p-Nitroaniline as Matrix for High Spatial Resolution Imaging of Phospholipids in Both Ion Modes by AP-MALDI-IMS

2014 ◽  
Vol 20 (S3) ◽  
pp. 2116-2117
Author(s):  
Shoko Matsushita ◽  
Eiji Sugiyama ◽  
Takahiro Hayasaka ◽  
Noritaka Masaki ◽  
Mitsutoshi Setou
Keyword(s):  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Maldi Ims ◽  
Resolution Imaging ◽  
High Spatial Resolution Imaging

scholarly journals Optimized Workflow for High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone

2021 ◽  
Author(s):  
Christopher J. Good ◽  
Elizabeth K. Neumann ◽  
Casey E. Butrico ◽  
James E. Cassat ◽  
Richard M. Caprioli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Ims ◽  
Fresh Frozen ◽  
Frozen Bone

Bone and bone marrow are vital to mammalian structure, movement, and immunity. These tissues are also commonly subjected to pathological alterations giving rise to debilitating diseases like rheumatoid arthritis, osteoporosis, osteomyelitis, and cancer. Technologies such as matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) enable the discovery of spatially resolved chemical information in biological tissue samples to help elucidate the complex molecular processes underlying pathology. Traditionally, preparation of native osseous tissue for MALDI IMS has been difficult due to the mineralized composition and heterogenous morphology of the tissue, and compensation for these challenges with decalcification and fixation protocols can remove or delocalize molecular species. Here, sample preparation methods were advanced to enable multimodal MALDI IMS of undecalcified, fresh-frozen murine femurs allowing the distribution of endogenous lipids to be linked to specific tissue structures and cell types. Adhesive-bound bone sections were mounted onto ITO coated glass slides with a microscopy-compatible glue and freeze-dried to minimize artificial bone marrow damage. Subliming matrix does not induce further bone marrow cracks, and recrystallizing the deposited matrix improves lipid signal. High spatial resolution (10 μm) MALDI IMS was leveraged to characterize lipid distributions in fresh-frozen bone, and complementary microscopy modalities aided tissue and cell assignments. For example, various phosphatidylcholines localize to bone marrow, adipose tissue, marrow adipose tissue, and muscle. Furthermore, we discovered that [sphingomyelin(42:1) + H]+ was abundant in megakaryocytes, whereas [sphingomyelin(42:2) + H]+ was diminished in this cell type. These data reflect the vast molecular and cellular heterogeneity indicative of the bone marrow and the soft tissue surrounding the femur. Therefore, this application of multimodal MALDI IMS has the potential to advance bone-related biomedical research by offering deep molecular coverage in a preserved native bone microenvironment.

High Spatial Resolution Soft X-Ray Microscopy and Microanalysis of Thick and Hydrated Materials

1998 ◽  
Vol 4 (S2) ◽  
pp. 352-353
Author(s):  
W. Meyer-Ilse ◽  
J. T. Brown ◽  
C. Magowan ◽  
J. Yeung ◽  
K. E. Kurtis ◽  
...  
Keyword(s):  
High Resolution ◽  
Spatial Resolution ◽  
Atmospheric Pressure ◽  
High Spatial Resolution ◽  
Ccd Camera ◽  
Optical Path ◽  
Zone Plate ◽  
Full Field ◽  
X Ray ◽  
Scientific Fields

The Center for X-ray Optics (CXRO) built and operates a high-resolution soft x-ray microscope (XM-1) at the Advanced Light Source in Berkeley. We report on the use of this instrument in a variety of scientific fields, including biology, civil engineering and environmental sciences.The microscope is a conventional (full field) x-ray microscope, which uses zone plate lenses to provide high resolution transmission images. The optical setup is similar to the Göttingen x-ray microscope, operated at the BESSY synchrotron radiation facility in Berlin, Germany. A condenser zone plate, fabricated by the Göttingen group, is illuminating the sample and an objective zone plate, fabricated by Erik Anderson (CXRO), is forming an enlarged image on an x-ray CCD camera. While the optical path of the microscope is in vacuum, the sample is at atmospheric pressure, flushed by helium. The spatial resolution of our microscope is 43 nm, measured as the distance from 10%-90% intensity in the image of a knife-edge.

High spatial resolution AEM observations of yttrium segregation in chromia scales grown on Co-45%Cr

1986 ◽  
Vol 44 ◽  
pp. 518-519
Author(s):  
K. Przybylski ◽  
A. J. Garratt-Reed ◽  
G. J. Yurek
Keyword(s):  
Spatial Resolution ◽  
Outer Surface ◽  
Maximum Concentration ◽  
High Spatial Resolution ◽  
Reactive Elements ◽  
Chromia Scales

The addition of so-called “reactive” elements such as yttrium to alloys is known to enhance the protective nature of Cr2O3 or Al2O3 scales. However, the mechanism by which this enhancement is achieved remains unclear. An A.E.M. study has been performed of scales grown at 1000°C for 25 hr. in pure O2 on Co-45%Cr implanted at 70 keV with 2x1016 atoms/cm2 of yttrium. In the unoxidized alloys it was calculated that the maximum concentration of Y was 13.9 wt% at a depth of about 17 nm. SIMS results showed that in the scale the yttrium remained near the outer surface.

Integration of Core-Edge Spectroscopy Methods for the Study of Polymers

1996 ◽  
Vol 54 ◽  
pp. 154-155
Author(s):  
E. G. Rightor
Keyword(s):  
Excited State ◽  
Polymer Blends ◽  
Gas Phase ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Electronic States ◽  
Core Electron ◽  
Bulk Solids ◽  
Development Application

Core edge spectroscopy methods are versatile tools for investigating a wide variety of materials. They can be used to probe the electronic states of materials in bulk solids, on surfaces, or in the gas phase. This family of methods involves promoting an inner shell (core) electron to an excited state and recording either the primary excitation or secondary decay of the excited state. The techniques are complimentary and have different strengths and limitations for studying challenging aspects of materials. The need to identify components in polymers or polymer blends at high spatial resolution has driven development, application, and integration of results from several of these methods.

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