Genetic Characterization of β-lactamase (VPA0477) in Vibrio parahaemolyticus

2011 ◽  
Vol 44 (6) ◽  
pp. 597-604 ◽  
Author(s):  
Nam-Hyung Lee ◽  
Hyun-Jung Song ◽  
Chang-Soo Park ◽  
Hee-Dai Kim ◽  
Kwon-Sam Park
Author(s):  
Kaknokrat Chonsin ◽  
Neunghatai Supha ◽  
Chie Nakajima ◽  
Yasuhiko Suzuki ◽  
Orasa Suthienkul

Abstract Vibrio parahaemolyticus (VP) is a major cause of gastroenteritis outbreaks in Thailand and other countries due to the consumption of contaminated and undercooked seafood. However, there have been few reports of the molecular epidemiology of VP isolates from asymptomatic seafood handlers. Here, we report the phenotypic and genetic characterization of 61 VP isolates obtained from asymptomatic workers in two seafood processing plants. We found 24 O:K serotypes of which O11:KUT, O1:KUT and O3:KUT were the dominant serotypes. Analysis by PCR showed 12 isolates harbored either tdh or trh genes with the potential to be pathogenic VP strains. The presence of T3SS2α and T3SS2β genes was correlated with the presence of tdh and trh, respectively. Four tdh+ isolates were positive for pandemic marker. In this study, VP isolates were commonly resistant to ampicillin, cephazolin, fosfomycin and novobiocin. Phylogenetic analysis of VP1680 loci in 35 isolates from 17 asymptomatic workers, six gastroenteritis patients, seven environmental samples and five genomes from a database showed 22 different alleles. Gene VP1680 was conserved in tdh+ isolates and pandemic strains, that of trh + isolates was diverse. Asymptomatic workers carrying VP were the most likely source of contamination, which raises concerns over food safety in seafood processing plants.


2016 ◽  
Vol 60 (11) ◽  
pp. 6965-6968 ◽  
Author(s):  
Ruichao Li ◽  
Lianwei Ye ◽  
Zhiwei Zheng ◽  
Edward Wai Chi Chan ◽  
Sheng Chen

ABSTRACTThis report describes the first detection of ablaVEB-2gene in aVibrio parahaemolyticusstrain isolated from a shrimp sample. TheblaVEB-2gene was carried on a novel Inc-type plasmid that was likely to have originated from aquatic organisms, as indicated by a comparison with other known genetic elements in the GenBank database. However, the plasmid contains resistance elements usually harbored by members of the familyEnterobacteriaceae, suggesting that gene transfer events occurred and contributed to the formation of this multidrug resistance-encoding plasmid.


2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.


Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  

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