scholarly journals Genetic Characterization of blaCTX–M–55 -Bearing Plasmids Harbored by Food-Borne Cephalosporin-Resistant Vibrio parahaemolyticus Strains in China

2019 ◽  
Vol 10 ◽  
Author(s):  
Zhiwei Zheng ◽  
Ruichao Li ◽  
Lianwei Ye ◽  
Edward Wai-chi Chan ◽  
Xiaodong Xia ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Genetic Characterization ◽  
Food Borne

Characterization of Vibrio parahaemolyticus strains isolated from clinically asymptomatic seafood workers

2020 ◽  
Author(s):  
Kaknokrat Chonsin ◽  
Neunghatai Supha ◽  
Chie Nakajima ◽  
Yasuhiko Suzuki ◽  
Orasa Suthienkul
Keyword(s):  
Phylogenetic Analysis ◽  
Food Safety ◽  
Vibrio Parahaemolyticus ◽  
Environmental Samples ◽  
Genetic Characterization ◽  
Processing Plants ◽  
Pandemic Strains ◽  
Phenotypic And Genetic Characterization ◽  
Seafood Processing

Abstract Vibrio parahaemolyticus (VP) is a major cause of gastroenteritis outbreaks in Thailand and other countries due to the consumption of contaminated and undercooked seafood. However, there have been few reports of the molecular epidemiology of VP isolates from asymptomatic seafood handlers. Here, we report the phenotypic and genetic characterization of 61 VP isolates obtained from asymptomatic workers in two seafood processing plants. We found 24 O:K serotypes of which O11:KUT, O1:KUT and O3:KUT were the dominant serotypes. Analysis by PCR showed 12 isolates harbored either tdh or trh genes with the potential to be pathogenic VP strains. The presence of T3SS2α and T3SS2β genes was correlated with the presence of tdh and trh, respectively. Four tdh+ isolates were positive for pandemic marker. In this study, VP isolates were commonly resistant to ampicillin, cephazolin, fosfomycin and novobiocin. Phylogenetic analysis of VP1680 loci in 35 isolates from 17 asymptomatic workers, six gastroenteritis patients, seven environmental samples and five genomes from a database showed 22 different alleles. Gene VP1680 was conserved in tdh+ isolates and pandemic strains, that of trh + isolates was diverse. Asymptomatic workers carrying VP were the most likely source of contamination, which raises concerns over food safety in seafood processing plants.

scholarly journals Characterization of a New Plasmid-Like Prophage in a Pandemic Vibrio parahaemolyticus O3:K6 Strain

2009 ◽  
Vol 75 (9) ◽  
pp. 2659-2667 ◽  
Author(s):  
Shih-Feng Lan ◽  
Chung-Ho Huang ◽  
Chuan-Hsiung Chang ◽  
Wei-Chao Liao ◽  
I-Hsuan Lin ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Open Reading Frames ◽  
Host Chromosome ◽  
Food Borne ◽  
Dna Adenine Methylase ◽  
Related Group ◽  
Bacterial Genes

ABSTRACT Vibrio parahaemolyticus is a common food-borne pathogen that is normally associated with seafood. In 1996, a pandemic O3:K6 strain abruptly appeared and caused the first pandemic of this pathogen to spread throughout many Asian countries, America, Europe, and Africa. The role of temperate bacteriophages in the evolution of this pathogen is of great interest. In this work, a new temperate phage, VP882, from a pandemic O3:K6 strain of V. parahaemolyticus was purified and characterized after mitomycin C induction. VP882 was a Myoviridae bacteriophage with a polyhedral head and a long rigid tail with a sheath-like structure. It infected and lysed high proportions of V. parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae strains. The genome of phage VP882 was sequenced and was 38,197 bp long, and 71 putative open reading frames were identified, of which 27 were putative functional phage or bacterial genes. VP882 had a linear plasmid-like genome with a putative protelomerase gene and cohesive ends. The genome does not integrate into the host chromosome but was maintained as a plasmid in the lysogen. Analysis of the reaction sites of the protelomerases in different plasmid-like phages revealed that VP882 and ΦHAP-1 were highly similar, while N15, ΦKO2, and PY54 made up another closely related group. The presence of DNA adenine methylase and quorum-sensing transcriptional regulators in VP882 may play a specific role in this phage or regulate physiological or virulence-associated traits of the hosts. These genes may also be remnants from the bacterial chromosome following transduction.

Genetic Characterization of β-lactamase (VPA0477) in Vibrio parahaemolyticus

2011 ◽  
Vol 44 (6) ◽  
pp. 597-604 ◽  
Author(s):  
Nam-Hyung Lee ◽  
Hyun-Jung Song ◽  
Chang-Soo Park ◽  
Hee-Dai Kim ◽  
Kwon-Sam Park
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Genetic Characterization

scholarly journals Genetic Characterization of ablaVEB-2-Carrying Plasmid in Vibrio parahaemolyticus

2016 ◽  
Vol 60 (11) ◽  
pp. 6965-6968 ◽  
Author(s):  
Ruichao Li ◽  
Lianwei Ye ◽  
Zhiwei Zheng ◽  
Edward Wai Chi Chan ◽  
Sheng Chen
Keyword(s):  
Multidrug Resistance ◽  
Gene Transfer ◽  
Vibrio Parahaemolyticus ◽  
Genetic Characterization ◽  
Aquatic Organisms ◽  
Content Type ◽  
Genetic Elements ◽  
Shrimp Sample ◽  
The Family

ABSTRACTThis report describes the first detection of ablaVEB-2gene in aVibrio parahaemolyticusstrain isolated from a shrimp sample. TheblaVEB-2gene was carried on a novel Inc-type plasmid that was likely to have originated from aquatic organisms, as indicated by a comparison with other known genetic elements in the GenBank database. However, the plasmid contains resistance elements usually harbored by members of the familyEnterobacteriaceae, suggesting that gene transfer events occurred and contributed to the formation of this multidrug resistance-encoding plasmid.

scholarly journals Prevalence and Characterization of Food-Borne Vibrio parahaemolyticus From African Salad in Southern Nigeria

2021 ◽  
Vol 12 ◽  
Author(s):  
Etinosa O. Igbinosa ◽  
Abeni Beshiru ◽  
Isoken H. Igbinosa ◽  
Abraham G. Ogofure ◽  
Kate E. Uwhuba
Keyword(s):  
Foodborne Pathogens ◽  
Vibrio Parahaemolyticus ◽  
Multidrug Resistant ◽  
Minimally Processed ◽  
No Formation ◽  
Food Borne ◽  
Southern Nigeria ◽  
Thermostable Direct Hemolysin ◽  
Minimally Processed Vegetables

The demand for minimally processed vegetables (African salad) has increased partly due to its inclusion in ready-to-eat foods. Nevertheless, the associated risk of the presence of emergent foodborne pathogens, such as Vibrio parahaemolyticus might be underestimated. The present study was designed to isolate and characterize foodborne V. parahaemolyticus from minimally processed vegetables using culture-based methods and molecular approach. A total of 300 samples were examined from retail outlets between November 2018 and August 2019 from Southern Nigeria. The prevalence of vibrios from the overall samples based on the colonial proliferation of yellow, blue-green and/or green colonies on thiosulfate citrate bile salts sucrose agar was 74/300 (24.6%). An average of two green or blue-green colonies from respective plates was screened for V. parahaemolyticus using analytical profile index (API) 20 NE. Polymerase chain reaction further confirmed the identity of positive V. parahaemolyticus. The counts of V. parahaemolyticus ranged from 1.5 to 1,000 MPN/g. A total of 63 recovered V. parahaemolyticus were characterized further. The resistance profile of the isolates include ampicillin 57/63 (90.5%), cefotaxime 41/63 (65.1%), ceftazidime 30/63 (47.6%), amikacin 32/63 (50.8%), kanamycin 15/63 (23.8%), and oxytetracycline 16/63 (25.4%). The multiple antibiotic index ranged from 0–0.81. The formation of biofilm by the isolates revealed the following: strong formation 15/63 (23.8%), moderate formation 31/63 (49.2%), weak formation 12/63 (19.1%), and no formation 5/63 (7.9%). A total of 63/63 (100%), 9/63 (14.3%), and 20/63 (31.8%) of the isolates harbored the tox R gene, TDH-related hemolysin (trh) and thermostable direct hemolysin (tdh) determinants respectively. The isolates with O2 serogroup were most prevalent via PCR. Isolates that were resistant to tetracycline, kanamycin, and chloramphenicol possessed resistant genes. The presence of multidrug-resistant vibrios in the minimally processed vegetables constitutes a public health risk and thus necessitates continued surveillance.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma
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