scholarly journals Genomic Organization, Intronic Duplications, and Promoter Characteristics of the Fast Skeletal Myosin Light Chain-2 Gene (mlc2f) from Javanese Ricefish Oryzias javanicus

2012 ◽  
Vol 15 (4) ◽  
pp. 325-335
Author(s):  
Sang Yoon Lee ◽  
Dong Soo Kim ◽  
Yoon Kwon Nam
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Genomic Organization ◽  
Oryzias Javanicus ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin

scholarly journals Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene.

1988 ◽  
Vol 8 (2) ◽  
pp. 1006-1009 ◽  
Author(s):  
M Shani ◽  
I Dekel ◽  
O Yoffe
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Developmental Regulation ◽  
Endogenous Gene ◽  
Temporal Regulation ◽  
Stage Specificity ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin ◽  
Transgenic Strains

The expression of the rat skeletal myosin light-chain 2 gene in two transgenic strains was tissue specific and stage specific. However, the temporal regulation during development of the transgene was different from that of the endogenous gene. Surprisingly, in one strain, the expression of the transgene was associated with a significant down-regulation of the endogenous gene. The possible mechanisms to account for the suppression of the endogenous gene and the potential implications of this suppression are discussed.

scholarly journals Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation

2001 ◽  
Vol 204 (17) ◽  
pp. 3009-3018 ◽  
Author(s):  
Katerina A. Moutou ◽  
Adelino V. M. Canario ◽  
Zissis Mamuris ◽  
Deborah M. Power
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
White Muscle ◽  
Sparus Aurata ◽  
Developmental Expression ◽  
Sea Bream ◽  
Cdna Clones ◽  
Age Related ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin

SUMMARY Two full-length cDNA clones encoding the skeletal myosin light chain 2 (MLC2; 1452bp) and myosin light chain 3 (MLC3; 972bp) were isolated from a cDNA library prepared from gilthead sea bream Sparus aurata larvae. The MLC2 cDNA encoded a predicted protein of 170 residues that was 79% identical to rabbit MLC2 over the entire length and 87% identical within the Ca2+-binding region. The deduced amino acid sequence of MLC3 was 153 residues in length and was 91% and 69% identical to the zebrafish and rabbit MLC3, respectively. Northern blot analysis revealed that in adults both transcripts were expressed in fast white muscle only. MLC2 appeared earlier in development: MLC2 transcripts were detectable from the beginning of segmentation, whereas MLC3 transcripts did not appear until 27h post-fertilisation. At this developmental stage, a second MLC2 transcript of 0.89 kilobase-pairs was present. MLCs exhibited a different age-related pattern of response to varied thyroidal states, which were experimentally induced by the administration of 1μgg−1bodymass of thyroxine (T4) or triiodothyronine (T3), or 5ngg−1bodymass of the hypothyroidal compound thiourea; MLC3 expression was not significantly affected, whereas levels of MLC2 transcripts were significantly elevated in the white muscle only of juvenile sea bream after administration of T4. Although the mechanism of thyroidal regulation of MLC expression remains unknown, the present results suggest that different regulatory mechanisms exist for different MLCs.

Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene

1988 ◽  
Vol 8 (2) ◽  
pp. 1006-1009
Author(s):  
M Shani ◽  
I Dekel ◽  
O Yoffe
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Developmental Regulation ◽  
Endogenous Gene ◽  
Temporal Regulation ◽  
Stage Specificity ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin ◽  
Transgenic Strains

The expression of the rat skeletal myosin light-chain 2 gene in two transgenic strains was tissue specific and stage specific. However, the temporal regulation during development of the transgene was different from that of the endogenous gene. Surprisingly, in one strain, the expression of the transgene was associated with a significant down-regulation of the endogenous gene. The possible mechanisms to account for the suppression of the endogenous gene and the potential implications of this suppression are discussed.

scholarly journals Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis

1994 ◽  
Vol 269 (24) ◽  
pp. 16961-16970
Author(s):  
S.W. Kubalak ◽  
W.C. Miller-Hance ◽  
T.X. O'Brien ◽  
E. Dyson ◽  
K.R. Chien
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain 2 ◽  
Atrial Myosin

The force dependence of isometric and concentric potentiation in mouse muscle with and without skeletal myosin light chain kinase

2015 ◽  
Vol 93 (1) ◽  
pp. 23-32 ◽  
Author(s):  
William Gittings ◽  
Harish Aggarwal ◽  
James T. Stull ◽  
Rene Vandenboom
Keyword(s):  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Isometric Force ◽  
Conditioning Stimulus ◽  
Mouse Muscle ◽  
Tetanic Force ◽  
Concentric Force ◽  
Skeletal Myosin ◽  
Contraction Type

The isometric potentiation associated with myosin phosphorylation is force dependent. The purpose of this study was to assess the influence of a pre-existing period of isometric force on the concentric force potentiation displayed by mouse muscles with and without the ability to phosphorylate myosin. We tested isometric (ISO) and concentric (CON) potentiation, as well as concentric potentiation after isometric force (ISO-CON), in muscles from wild-type (WT) and skeletal myosin light chain kinase-deficient (skMLCK−/−) mice. A conditioning stimulus increased (i.e., potentiated) mean concentric force in the ISO-CON and CON conditions to 1.31 ± 0.02 and 1.35 ± 0.02 (WT) and to 1.19 ± 0.02 and 1.21 ± 0.01 (skMLCK−/−) of prestimulus levels, respectively (data n = 6–8, p < 0.05). No potentiation of mean isometric force was observed in either genotype. The potentiation of mean concentric force was inversely related to relative tetanic force level (P/Po) in both genotypes. Moreover, concentric potentiation varied greatly within each contraction type and was negatively correlated with unpotentiated force in both genotypes. Thus, although no effect of pre-existing force was observed, strong and inverse relationships between concentric force potentiation and unpotentiated concentric force may suggest an influence of attached and force-generating crossbridges on potentiation magnitude in both WT and skMLCK−/− muscles.

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