Expression of skeletal myosin light chain 2 in gilthead sea bream (Sparus aurata, L)

2013 ◽  
Author(s):  
Styliani Georgiou
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin

Myosin light chain 2 isoforms in gilthead sea bream (Sparus aurata L.): Molecular growth markers at early stages

Aquaculture ◽  
2014 ◽  
Vol 432 ◽  
pp. 434-442 ◽  
Author(s):  
Stella Georgiou ◽  
Pavlos Makridis ◽  
Dimitris Dimopoulos ◽  
Deborah M. Power ◽  
Zissis Mamuris ◽  
...  
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Early Stages ◽  
Myosin Light Chain 2

scholarly journals Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation

2001 ◽  
Vol 204 (17) ◽  
pp. 3009-3018 ◽  
Author(s):  
Katerina A. Moutou ◽  
Adelino V. M. Canario ◽  
Zissis Mamuris ◽  
Deborah M. Power
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
White Muscle ◽  
Sparus Aurata ◽  
Developmental Expression ◽  
Sea Bream ◽  
Cdna Clones ◽  
Age Related ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin

SUMMARY Two full-length cDNA clones encoding the skeletal myosin light chain 2 (MLC2; 1452bp) and myosin light chain 3 (MLC3; 972bp) were isolated from a cDNA library prepared from gilthead sea bream Sparus aurata larvae. The MLC2 cDNA encoded a predicted protein of 170 residues that was 79% identical to rabbit MLC2 over the entire length and 87% identical within the Ca2+-binding region. The deduced amino acid sequence of MLC3 was 153 residues in length and was 91% and 69% identical to the zebrafish and rabbit MLC3, respectively. Northern blot analysis revealed that in adults both transcripts were expressed in fast white muscle only. MLC2 appeared earlier in development: MLC2 transcripts were detectable from the beginning of segmentation, whereas MLC3 transcripts did not appear until 27h post-fertilisation. At this developmental stage, a second MLC2 transcript of 0.89 kilobase-pairs was present. MLCs exhibited a different age-related pattern of response to varied thyroidal states, which were experimentally induced by the administration of 1μgg−1bodymass of thyroxine (T4) or triiodothyronine (T3), or 5ngg−1bodymass of the hypothyroidal compound thiourea; MLC3 expression was not significantly affected, whereas levels of MLC2 transcripts were significantly elevated in the white muscle only of juvenile sea bream after administration of T4. Although the mechanism of thyroidal regulation of MLC expression remains unknown, the present results suggest that different regulatory mechanisms exist for different MLCs.

scholarly journals Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene.

1988 ◽  
Vol 8 (2) ◽  
pp. 1006-1009 ◽  
Author(s):  
M Shani ◽  
I Dekel ◽  
O Yoffe
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Developmental Regulation ◽  
Endogenous Gene ◽  
Temporal Regulation ◽  
Stage Specificity ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin ◽  
Transgenic Strains

The expression of the rat skeletal myosin light-chain 2 gene in two transgenic strains was tissue specific and stage specific. However, the temporal regulation during development of the transgene was different from that of the endogenous gene. Surprisingly, in one strain, the expression of the transgene was associated with a significant down-regulation of the endogenous gene. The possible mechanisms to account for the suppression of the endogenous gene and the potential implications of this suppression are discussed.

Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene

1988 ◽  
Vol 8 (2) ◽  
pp. 1006-1009
Author(s):  
M Shani ◽  
I Dekel ◽  
O Yoffe
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Developmental Regulation ◽  
Endogenous Gene ◽  
Temporal Regulation ◽  
Stage Specificity ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin ◽  
Transgenic Strains

The expression of the rat skeletal myosin light-chain 2 gene in two transgenic strains was tissue specific and stage specific. However, the temporal regulation during development of the transgene was different from that of the endogenous gene. Surprisingly, in one strain, the expression of the transgene was associated with a significant down-regulation of the endogenous gene. The possible mechanisms to account for the suppression of the endogenous gene and the potential implications of this suppression are discussed.

Stress effects of amyloodiniosis in gilthead sea bream Sparus aurata

2018 ◽  
Vol 127 (3) ◽  
pp. 201-211 ◽  
Author(s):  
M Moreira ◽  
M Herrera ◽  
P Pousão-Ferreira ◽  
JI Navas Triano ◽  
F Soares
Keyword(s):  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Stress Effects

scholarly journals Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis

1994 ◽  
Vol 269 (24) ◽  
pp. 16961-16970
Author(s):  
S.W. Kubalak ◽  
W.C. Miller-Hance ◽  
T.X. O'Brien ◽  
E. Dyson ◽  
K.R. Chien
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain 2 ◽  
Atrial Myosin
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