scholarly journals Gas Fermentation of Chemicals

Oleoscience ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 417-424
Author(s):  
Junya KATO ◽  
Yutaka NAKASHIMADA
Keyword(s):  
Gas Fermentation

scholarly journals Metabolic engineering of Moorella thermoacetica for thermophilic bioconversion of gaseous substrates to a volatile chemical

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Junya Kato ◽  
Kaisei Takemura ◽  
Setsu Kato ◽  
Tatsuya Fujii ◽  
Keisuke Wada ◽  
...  
Keyword(s):  
Carbon Flux ◽  
Cost Effective ◽  
Gas Composition ◽  
Autotrophic Growth ◽  
Acetyl Coenzyme A ◽  
Volatile Chemicals ◽  
Moorella Thermoacetica ◽  
Gas Fermentation ◽  
Dry Cell ◽  
Co Gas

AbstractGas fermentation is one of the promising bioprocesses to convert CO2 or syngas to important chemicals. Thermophilic gas fermentation of volatile chemicals has the potential for the development of consolidated bioprocesses that can simultaneously separate products during fermentation. This study reports the production of acetone from CO2 and H2, CO, or syngas by introducing the acetone production pathway using acetyl–coenzyme A (Ac-CoA) and acetate produced via the Wood–Ljungdahl pathway in Moorella thermoacetica. Reducing the carbon flux from Ac-CoA to acetate through genetic engineering successfully enhanced acetone productivity, which varied on the basis of the gas composition. The highest acetone productivity was obtained with CO–H2, while autotrophic growth collapsed with CO2–H2. By adding H2 to CO, the acetone productivity from the same amount of carbon source increased compared to CO gas only, and the maximum specific acetone production rate also increased from 0.04 to 0.09 g-acetone/g-dry cell/h. Our development of the engineered thermophilic acetogen M. thermoacetica, which grows at a temperature higher than the boiling point of acetone (58 °C), would pave the way for developing a consolidated process with simplified and cost-effective recovery via condensation following gas fermentation.

scholarly journals Transcriptional Control of Clostridium autoethanogenum using CRISPRi

2021 ◽  
Author(s):  
Nick Fackler ◽  
James Heffernan ◽  
Alex Juminaga ◽  
Damien Doser ◽  
Shilpa Nagaraju ◽  
...  
Keyword(s):  
Transcriptional Repression ◽  
Transcriptional Control ◽  
Genome Engineering ◽  
Low Cost ◽  
Pathway Engineering ◽  
Industrial Emissions ◽  
Gas Fermentation ◽  
Control Gene Expression ◽  
Clostridium Autoethanogenum ◽  
Commercial Process

Abstract Gas fermentation by Clostridium autoethanogenum is a commercial process for the sustainable biomanufacturing of fuels and valuable chemicals using abundant, low cost C1 feedstocks (CO and CO2) from sources such as inedible biomass, unsorted and non-recyclable municipal solid waste, and industrial emissions. Efforts towards pathway engineering and elucidation of gene function in this microbe have been limited by a lack of genetic tools to control gene expression and arduous genome engineering methods. To increase the pace of progress, here we developed an inducible CRISPR interference (CRISPRi) system for C. autoethanogenum and applied that system towards transcriptional repression of genes with ostensibly crucial functions in metabolism.

Methanol supply speeds up synthesis gas fermentation by methylotrophic-acetogenic bacterium, Eubacterium limosum KIST612

2021 ◽  
Vol 321 ◽  
pp. 124521
Author(s):  
Ji-Yeon Kim ◽  
Sehoon Park ◽  
Jiyeong Jeong ◽  
Mungyu Lee ◽  
Byeongchan Kang ◽  
...  
Keyword(s):  
Synthesis Gas ◽  
Gas Fermentation ◽  
Eubacterium Limosum ◽  
Acetogenic Bacterium ◽  
Synthesis Gas Fermentation

Bioeconomy of municipal solid waste (MSW) using gas fermentation

2020 ◽  
pp. 289-304
Author(s):  
Khushboo ◽  
Ankush ◽  
Karuna Yadav ◽  
Mrinal Kanti Mandal ◽  
Supriya Pal ◽  
...  
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Gas Fermentation

scholarly journals Reconstruction of an Acetogenic 2,3-Butanediol Pathway Involving a Novel NADPH-Dependent Primary-Secondary Alcohol Dehydrogenase

2014 ◽  
Vol 80 (11) ◽  
pp. 3394-3403 ◽  
Author(s):  
Michael Köpke ◽  
Monica L. Gerth ◽  
Danielle J. Maddock ◽  
Alexander P. Mueller ◽  
FungMin Liew ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Secondary Alcohol ◽  
Acetolactate Synthase ◽  
Chemical Production ◽  
Content Type ◽  
E Coli ◽  
Gas Fermentation ◽  
Secondary Alcohol Dehydrogenase ◽  
Clostridium Autoethanogenum ◽  
Butanediol Dehydrogenase

ABSTRACTAcetogenic bacteria use CO and/or CO2plus H2as their sole carbon and energy sources. Fermentation processes with these organisms hold promise for producing chemicals and biofuels from abundant waste gas feedstocks while simultaneously reducing industrial greenhouse gas emissions. The acetogenClostridium autoethanogenumis known to synthesize the pyruvate-derived metabolites lactate and 2,3-butanediol during gas fermentation. Industrially, 2,3-butanediol is valuable for chemical production. Here we identify and characterize theC. autoethanogenumenzymes for lactate and 2,3-butanediol biosynthesis. The putativeC. autoethanogenumlactate dehydrogenase was active when expressed inEscherichia coli. The 2,3-butanediol pathway was reconstituted inE. coliby cloning and expressing the candidate genes for acetolactate synthase, acetolactate decarboxylase, and 2,3-butanediol dehydrogenase. Under anaerobic conditions, the resultingE. colistrain produced 1.1 ± 0.2 mM 2R,3R-butanediol (23 μM h−1optical density unit−1), which is comparable to the level produced byC. autoethanogenumduring growth on CO-containing waste gases. In addition to the 2,3-butanediol dehydrogenase, we identified a strictly NADPH-dependent primary-secondary alcohol dehydrogenase (CaADH) that could reduce acetoin to 2,3-butanediol. Detailed kinetic analysis revealed that CaADH accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone. The high activity of CaADH toward acetone led us to predict, and confirm experimentally, thatC. autoethanogenumcan act as a whole-cell biocatalyst for converting exogenous acetone to isopropanol. Together, our results functionally validate the 2,3-butanediol pathway fromC. autoethanogenum, identify CaADH as a target for further engineering, and demonstrate the potential ofC. autoethanogenumas a platform for sustainable chemical production.

scholarly journals Gas fermentation for commodity chemicals and fuels

2017 ◽  
Vol 10 (5) ◽  
pp. 1167-1170 ◽  
Author(s):  
Frank R. Bengelsdorf ◽  
Peter Dürre
Keyword(s):  
Gas Fermentation ◽  
Commodity Chemicals
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