scholarly journals Control of Lipid Self-Assembled Structures & Assessment of Lipid Membrane Fluidity by Fluorescence Spectroscopy

2020 ◽  
Vol 69 (2) ◽  
pp. 83-91
Author(s):  
Makoto Uyama
Keyword(s):  
Fluorescence Spectroscopy ◽  
Membrane Fluidity ◽  
Lipid Membrane ◽  
Self Assembled

scholarly journals Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes

2021 ◽  
Author(s):  
Alexander Flegler ◽  
Vanessa Kombeitz ◽  
André Lipski
Keyword(s):  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Membrane Fluidity ◽  
Low Temperatures ◽  
Lipid Membrane ◽  
Feedback Inhibition ◽  
Aromatic Amino Acids ◽  
Adaptation Effect ◽  
Membrane Composition ◽  
Lower Growth

AbstractListeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen.

Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 65684-65689 ◽  
Author(s):  
Fan Xu ◽  
Meimei Bao ◽  
Longfei Rui ◽  
Jiaojiao Liu ◽  
Jingliang Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Surface Coating ◽  
Lipid Membrane ◽  
Coating Film ◽  
Silk Fibroin Film ◽  
Bombyx Mori Silk ◽  
Self Assembled

A self-assembled lipid membrane provides a smooth, hydrophilic and biocompatible surface coating film for materials.

Changes of erythrocyte membrane fluidity associated with childhood obesity: A molecular study using fluorescence spectroscopy

1988 ◽  
Vol 40 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Gianna Ferretti ◽  
Maria Dotti ◽  
Edoardo Bartolotta ◽  
Pier Luigi Giorgi ◽  
Giovanna Curatola ◽  
...  
Keyword(s):  
Childhood Obesity ◽  
Fluorescence Spectroscopy ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Molecular Study ◽  
Erythrocyte Membrane Fluidity

An increase in model lipid membrane fluidity as a result of local anesthetic action

2016 ◽  
Vol 10 (4) ◽  
pp. 324-331 ◽  
Author(s):  
S. S. Efimova ◽  
R. Ya. Medvedev ◽  
L. V. Schagina ◽  
O. S. Ostroumova
Keyword(s):  
Membrane Fluidity ◽  
Local Anesthetic ◽  
Lipid Membrane ◽  
Anesthetic Action

scholarly journals A self-assembled cyclodextrin nanocarrier for photoreactive squaraine

2016 ◽  
Vol 12 ◽  
pp. 2535-2542 ◽  
Author(s):  
Ulrike Kauscher ◽  
Bart Jan Ravoo
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Spectroscopy ◽  
Absorption Spectroscopy ◽  
Photochemical Activity ◽  
Oxygen Species ◽  
Significant Potential ◽  
Self Assembled

Photoreactive squaraines produce cytotoxic oxygen species under irradiation and have significant potential for photodynamic therapy. Herein we report that squaraines can be immobilized on a self-assembled nanocarrier composed of amphiphilic cyclodextrins to enhance their photochemical activity. To this end, a squaraine was equipped with two adamantane moieties that act as anchors for the cyclodextrin vesicle surface. The supramolecular immobilization was monitored by using fluorescence spectroscopy and microscopy and the photochemistry of the squaraine was investigated by using absorption spectroscopy.

Supported Bilayer Lipid Membrane Arrays on Photopatterned Self-Assembled Monolayers

2007 ◽  
Vol 13 (28) ◽  
pp. 7957-7964 ◽  
Author(s):  
Xiaojun Han ◽  
Singh N. D. Pradeep ◽  
Kevin Critchley ◽  
Khizar Sheikh ◽  
Richard J. Bushby ◽  
...  
Keyword(s):  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Self Assembled Monolayers ◽  
Bilayer Lipid ◽  
Supported Bilayer ◽  
Assembled Monolayers ◽  
Self Assembled

Biomembrane induced in situ self-assembly of peptide with enhanced antimicrobial activity

2020 ◽  
Vol 8 (7) ◽  
pp. 2031-2039 ◽  
Author(s):  
Zhiwei Shen ◽  
Zhen Guo ◽  
Limin Zhou ◽  
Yujiao Wang ◽  
Jinjin Zhang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Self Assembly ◽  
Lipid Membrane ◽  
Self Assembled

FF8 self-assembled into nanofibers on the negatively charged lipid membrane and induced rupture of the membrane.

scholarly journals Supramolecular exo-functionalized palladium cages: fluorescent properties and biological activity

2016 ◽  
Vol 45 (20) ◽  
pp. 8556-8565 ◽  
Author(s):  
Andrea Schmidt ◽  
Manuela Hollering ◽  
Markus Drees ◽  
Angela Casini ◽  
Fritz E. Kühn
Keyword(s):  
Biological Activity ◽  
Fluorescence Spectroscopy ◽  
Dft Calculations ◽  
Anticancer Activities ◽  
Fluorescent Properties ◽  
Emission Properties ◽  
Coordination Cages ◽  
Self Assembled

Self-assembled Pd(ii) coordination cages exhibit promising anticancer activities, while the emission properties studied by fluorescence spectroscopy and DFT calculations are limited.

scholarly journals Impact of selected polyphenolics on the structural properties of model lipid membranes – a review

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Nataša P. Ulrih ◽  
Ajda Ota ◽  
Veronika Abram
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Fluidity ◽  
Phenolic Acids ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Biological Effects ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Model Lipid Membranes ◽  
Electron Paramagnetic

This review is a presentation of data gathered on the interactions of several polyphenolics (i.e., phenolic acids, stilbenes, flavonoids) with lipid bilayers of different lipid compositions. These polyphenolics have been investigated through a combination of fluorescence spectroscopy, electron paramagnetic resonance spectroscopy, and differential scanning calorimetry, to detect changes in membrane fluidity. Among the investigated phenolic acids, the least polar phenolic acid, p-coumaric acid, has the greatest effect on lipid membrane structure. It appears to have a greater ability to cross membranes by passive transport than more polar phenolic acids. On the other hand, among the flavonoids that have been studied, the anthocyanins cyanidin 3-glucoside and its aglycone are inactive. All of the flavonols tested, except for epigallocatechin-3-gallate, promote small decreases in membrane fluidity. Computer simulation of electron paramagnetic resonance spectra for flavonoids indicated two or three regions in the phosphatidylcholine/ phosphatidylserine (2.4:1) membrane with different fluidity characteristics. The effects of the different flavonoids are correlated to their structural characteristics, whereby even the difference in one -OH group can be important, as can the number of H-bonds they form. The role of membrane composition and flavonoid structure in these interactions with lipid membranes are of great importance for bioavailability of these compounds and for their biological effects in an organism

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