scholarly journals Expression and Characterization of a Class III Alcohol Dehydrogenase Gene from Gluconobacter frateurii in the Presence of Methanol during Glyceric Acid Production from Glycerol

2013 ◽  
Vol 62 (10) ◽  
pp. 835-842 ◽  
Author(s):  
Shun Sato ◽  
Naoki Morita ◽  
Dai Kitamoto ◽  
Hiroshi Habe
Keyword(s):  
Alcohol Dehydrogenase ◽  
Acid Production ◽  
Glyceric Acid ◽  
Class Iii ◽  
Dehydrogenase Gene

scholarly journals Membrane-Bound Alcohol Dehydrogenase Is Essential for Glyceric Acid Production in Acetobacter tropicalis

2011 ◽  
Vol 60 (9) ◽  
pp. 489-494 ◽  
Author(s):  
Hiroshi Habe ◽  
Shun Sato ◽  
Tokuma Fukuoka ◽  
Dai Kitamoto ◽  
Toshiharu Yakushi ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Acid Production ◽  
Glyceric Acid ◽  
Membrane Bound

Cloning and Characterization of a Novel Human Alcohol Dehydrogenase Gene (ADHFe1)

DNA Sequence ◽  
2002 ◽  
Vol 13 (5) ◽  
pp. 301-306 ◽  
Author(s):  
Yingfeng Deng ◽  
Zhao Wang ◽  
Shaohua Gu ◽  
Chaoneng Ji ◽  
Kang Ying ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Gene

scholarly journals Cloning and Expression of ntnD, Encoding a Novel NAD(P)+-Independent 4-Nitrobenzyl Alcohol Dehydrogenase from Pseudomonas sp. Strain TW3

2000 ◽  
Vol 182 (11) ◽  
pp. 3136-3141 ◽  
Author(s):  
Keith D. James ◽  
Michelle A. Hughes ◽  
Peter A. Williams
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Alcohol Dehydrogenase ◽  
Benzyl Alcohol ◽  
Cloning And Expression ◽  
Significant Similarity ◽  
Reverse Transcription Pcr ◽  
Nitrobenzyl Alcohol ◽  
Dehydrogenase Gene

ABSTRACT Pseudomonas sp. strain TW3 is able to metabolize 4-nitrotoluene to 4-nitrobenzoate and toluene to benzoate aerobically via a route analogous to the upper pathway of the TOL plasmids. We report the cloning and characterization of a benzyl alcohol dehydrogenase gene (ntnD) which encodes the enzyme for the catabolism of 4-nitrobenzyl alcohol and benzyl alcohol to 4-nitrobenzaldehyde and benzaldehyde, respectively. The gene is located downstream of the previously reported ntn gene cluster. NtnD bears no similarity to the analogous TOL plasmid XylB (benzyl alcohol dehydrogenase) protein either in its biochemistry, being NAD(P)+ independent and requiring assay via dye-linked electron transfer, or in its deduced amino acid sequence. It does, however, have significant similarity in its amino acid sequence to other NAD(P)+-independent alcohol dehydrogenases and contains signature patterns characteristic of type III flavin adenine dinucleotide-dependent alcohol oxidases. Reverse transcription-PCR demonstrated that ntnD is transcribed during growth on 4-nitrotoluene, although apparently not as part of the same transcript as the other ntn genes. The substrate specificity of the enzyme expressed from the cloned and overexpressed gene was similar to the activity expressed from strain TW3 grown on 4-nitrotoluene, providing evidence that ntnD is the previously unidentified gene in the pathway of 4-nitrotoluene catabolism. Examination of the 14.8-kb region around the ntn genes suggests that one or more recombination events have been involved in the formation of their current organization.

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