scholarly journals Isolation and in silico characterization of full-length cinnamyl alcohol dehydrogenase gene involved in lignin biosynthesis in Neolamarckia cadamba

2018 ◽  
Keyword(s):  
Alcohol Dehydrogenase ◽  
In Silico ◽  
Lignin Biosynthesis ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Full Length ◽  
Cinnamyl Alcohol ◽  
Dehydrogenase Gene ◽  
In Silico Characterization

scholarly journals Isolation and Characterization of Full-Length Phenylalanine Ammonium Lyase and Cinnamyl Alcohol Dehydrogenase Genes Involved in Lignin Biosynthesis of Erianthus Arundinaceus

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 174
Author(s):  
Lakshmi Kasirajan ◽  
Prathima Perumal Thirugnanasambandam ◽  
Agnelo Furtado ◽  
Frikkie C. Botha ◽  
Robert J. Henry
Keyword(s):  
Alcohol Dehydrogenase ◽  
Untranslated Region ◽  
Plant Cell Wall ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Raw Material ◽  
Sequence Information ◽  
Cinnamyl Alcohol ◽  
Isolation And Characterization

Lignocellulosic biomasses available in abundance is the most promising raw material for alternate energy production considering the issues of dwindling oil prices, and global warming. Recently, Erianthus arundinaceous has been identified as a potential target for second generation biofuel crop due to its high biomass production, and adaptability to extreme growth environments. Lignin is a major plant cell wall polymer indispensable for plant growth and development, however it hinders the saccharification of lignocellulosic biomass. Based on the previous transcriptome studies in a set of sugarcane genotypes differing for lignin content, genes encoding cinnamyl alcohol dehydrogenase (CAD), and Phenylalanine ammonia lyase (PAL) genes playing major roles in genetic regulation of lignin production have been cloned and characterized from an Erianthus clone IK 76-81. The genomic region of EriCAD was 3524 bp sequence containing four exons and three introns, among which the exon 1&2 of 88 and 80 bp were conserved with sorghum and Miscanthus CADs. The coding region of CAD was identified with 1086 bp open reading frame (ORF), a 68 bp 5′ untranslated region (UTR), and a 86 bp 3′ untranslated region (UTR). In the PROSITE analysis, a zinc-containing alcohol dehydrogenase signature (GHEVVGEVVEVGPEV) and an NADP-binding domain motif (GLGGLG) was identified. Similarly sequence analysis of PAL showed an ORF of 2106 bp encoding for 702 amino acid residues. It was flanked by 172 bp of 5′ UTR and 121 bp of 3′ UTR. This sequence information on PAL and CAD from Erianthus might be useful for subsequent research on lignin modification for improved biomass conversion.

The cinnamyl alcohol dehydrogenase gene structure in

Trees ◽  
1998 ◽  
Vol 12 (8) ◽  
pp. 453
Author(s):  
R. Schubert ◽  
Christoph Sperisen ◽  
Gerhard Müller-Starck ◽  
Sabina La Scala ◽  
Dieter Ernst ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gene Structure ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol ◽  
Dehydrogenase Gene

Transposon insertion in a cinnamyl alcohol dehydrogenase gene is responsible for a brown midrib1 mutation in maize

2012 ◽  
Vol 80 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Wei Chen ◽  
Nathan VanOpdorp ◽  
Dennis Fitzl ◽  
Jagdish Tewari ◽  
Peter Friedemann ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol ◽  
Transposon Insertion ◽  
Dehydrogenase Gene
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