scholarly journals Characterization of Sterol Lipids in Kluyveromyces lactis Strain M-16 Accumulating a High Amount of Steryl Glucoside

2009 ◽  
Vol 58 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Michiko Sugai ◽  
Naoya Takakuwa ◽  
Masao Ohnishi ◽  
Tadasu Urashima ◽  
Yuji Oda
Keyword(s):  
Kluyveromyces Lactis ◽  
Steryl Glucoside ◽  
Lactis Strain

Partial characterization of cheese-ripening proteinases produced by the yeastKluyveromyces lactis

1983 ◽  
Vol 50 (4) ◽  
pp. 469-480 ◽  
Author(s):  
Paul A. Grieve ◽  
Barry J. Kitchen ◽  
John R. Dulley ◽  
John Bartley
Keyword(s):  
Molecular Weight ◽  
Kluyveromyces Lactis ◽  
Apparent Molecular Weight ◽  
Aminopeptidase Activity ◽  
Carboxypeptidase Y ◽  
Casein Hydrolysis ◽  
Cheese Ripening ◽  
Caseinolytic Activity ◽  
Proteinase A

SUMMARYAn extract ofKluyveromyces lactis416 and a β-galactosidase preparation (Maxilact 40000) contaminated with proteinase, showed similar pH profiles of caseinolytic activity. Similar modes of casein hydrolysis (κ-, > αs-, ≥ β-) were observed at pH 5·0 (the pH of Cheddar cheese), without detection of bitterness. The contaminated Maxilact preparation contained similar proteinase types to those detected in an autolysate ofK. lactis. Both the autolysate and the Maxilact preparation contained acid endopeptidase (proteinase A), serine endopeptidase (proteinase B) and serine exopeptidase (carboxypeptidase Y) activities. Some aminopeptidase activity was also detected in both preparations. There were some differences in apparent molecular weight and charge properties between proteinase A and B and carboxypeptidase Y from the 2 proteinase sources.

scholarly journals Characterization of a Lactococcus lactis Strain That Secretes a Major Epitope of Bovine Beta-Lactoglobulin and Evaluation of Its Immunogenicity in Mice

2003 ◽  
Vol 69 (11) ◽  
pp. 6620-6627 ◽  
Author(s):  
Jean-Marc Chatel ◽  
Sebastien Nouaille ◽  
Karine Adel-Patient ◽  
Yves Le Loir ◽  
Herman Boe ◽  
...  
Keyword(s):  
Immune Response ◽  
Fusion Protein ◽  
Lactococcus Lactis ◽  
Immunoglobulin E ◽  
Specific Ige ◽  
Specific Immune Response ◽  
Lactis Strain ◽  
Beta Lactoglobulin ◽  
Β Lactoglobulin

ABSTRACT Bovine β-lactoglobulin (Blg) is one of the major cow's milk allergens. Peptide 41-60 of Blg (Blg41-60) was described as a murine T-cell determinant and a murine, rat, and human immunoglobulin E (IgE) epitope. The aim of this study was the expression of Blg41-60 as a fusion protein in the food-grade bacterium Lactococcus lactis and the characterization of its immunogenicity in mice. We constructed a recombinant strain of L. lactis capable of inducible production and secretion of Blg41-60::Nuc, a fusion protein between Blg41-60 and the mature part of the staphylococcal nuclease (Nuc). The highest production yield of Blg41-60::Nuc (32.5 mg/liter) was reached 4 h after induction. At this time, up to 75% of Blg41-60::Nuc was secreted. When monoclonal antibodies specific for Blg41-60 were used, purified Blg41-60::Nuc and synthetic Blg41-60 exhibited very similar immunoreactivities. Subcutaneous coadministration of purified Blg41-60::Nuc and killed nonrecombinant L. lactis resulted in the induction of specific anti-Blg41-60 IgG2a and IgG1. The IgG1/IgG2a ratio and the lack of specific IgE suggest a Th1-type immune response, i.e., a nonallergic response. Similar administrations of the killed Blg41-60::Nuc-producing L. lactis strain did not elicit a specific immune response, whereas a transitory mucosal IgA-specific immune response was induced in mice after oral administration of the live Blg41-60::Nuc-producing L. lactis strain.

scholarly journals Separation of Soybean Sterols by Florisil Chromatography and Characterization of Acylated Steryl Glucoside

1966 ◽  
Vol 30 (8) ◽  
pp. 770-778 ◽  
Author(s):  
Toshiko KIRIBUCHI ◽  
Takemitsu MIZUNAGA ◽  
Saburo FUNAHASHI
Keyword(s):  
Steryl Glucoside

scholarly journals Comparative biochemical characterization of soluble and chitosan immobilized β-galactosidase from Kluyveromyces lactis NRRL Y1564

2013 ◽  
Vol 48 (3) ◽  
pp. 443-452 ◽  
Author(s):  
Ariosvana Fernandes Lima ◽  
Kenia Franco Cavalcante ◽  
Maria de Fátima Matos de Freitas ◽  
Tigressa Helena Soares Rodrigues ◽  
Maria Valderez Ponte Rocha ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Kluyveromyces Lactis

Isolation and characterization of mutants of Kluyveromyces lactis defective in lactose transport

1987 ◽  
Vol 208 (1-2) ◽  
pp. 145-151 ◽  
Author(s):  
Michael I. Riley ◽  
K. Sreekrishna ◽  
Srirama Bhairi ◽  
Robert C. Dickson
Keyword(s):  
Kluyveromyces Lactis ◽  
Isolation And Characterization ◽  
Lactose Transport
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