scholarly journals Suppressive Effects of Alloxanthin and Diatoxanthin from Halocynthia roretzi on LPS-induced Expression of Pro-inflammatory Genes in RAW264.7 Cells

2008 ◽  
Vol 57 (3) ◽  
pp. 181-189 ◽  
Author(s):  
Izumi Konishi ◽  
Masashi Hosokawa ◽  
Tokutake Sashima ◽  
Takashi Maoka ◽  
Kazuo Miyashita
Keyword(s):  
Raw264.7 Cells ◽  
Halocynthia Roretzi ◽  
Induced Expression ◽  
Inflammatory Genes

scholarly journals Lactoferrin suppresses LPS-induced expression of HMGB1, microRNA 155, 146, and TLR4/MyD88/NF-кB pathway in RAW264.7 cells

2021 ◽  
pp. 1-11
Author(s):  
Maryam Nemati ◽  
Saeideh Akseh ◽  
Maryam Amiri ◽  
Hamid Reza Nejabati ◽  
Ahmadreza Jodati ◽  
...  
Keyword(s):  
Raw264.7 Cells ◽  
Induced Expression

Loperamide-induced expression of immune and inflammatory genes in Collies associated with ivermectin sensitivity

2015 ◽  
Vol 39 (2) ◽  
pp. 131-137 ◽  
Author(s):  
M. Zhu ◽  
H. F. Yancy ◽  
C. Deaver ◽  
Y. L. Jones ◽  
M. J. Myers
Keyword(s):  
Induced Expression ◽  
Inflammatory Genes

Differential effects of NF-κB and p38 MAPK inhibitors and combinations thereof on TNF-α- and IL-1β-induced proinflammatory status of endothelial cells in vitro

2005 ◽  
Vol 289 (5) ◽  
pp. C1229-C1239 ◽  
Author(s):  
Joanna M. Kułdo ◽  
Johanna Westra ◽  
Sigridur A. Ásgeirsdóttir ◽  
Robbert J. Kok ◽  
Koen Oosterhuis ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
P38 Mapk ◽  
Umbilical Vein ◽  
Combined Treatment ◽  
Dominant Negative ◽  
Mrna Levels ◽  
Induced Expression ◽  
Inflammatory Genes ◽  
Tnf Α ◽  
Cox 2

Endothelial cells actively participate in inflammatory events by regulating leukocyte recruitment via the expression of inflammatory genes such as E-selectin, VCAM-1, ICAM-1, IL-6, IL-8, and cyclooxygenase (COX)-2. In this study we showed by real-time RT-PCR that activation of human umbilical vein endothelial cells (HUVEC) by TNF-α and IL-1β differentially affected the expression of these inflammatory genes. Combined treatment with TNF-α and IL-1β resulted in nonadditive, additive, and even synergistic induction of expression of VCAM-1, IL-8, and IL-6, respectively. Overexpression of dominant-negative inhibitor κB protein blocking NF-κB signaling confirmed a major role of this pathway in controlling both TNF-α- and IL-1β-induced expression of most of the genes studied. Although dexamethasone exerted limited effects at 1 μM, the thioredoxin inhibitor MOL-294, which regulates the redox state of NF-κB, mainly inhibited adhesion molecule expression. Its most pronounced effect was seen on VCAM-1 mRNA levels, especially in IL-1β-activated endothelium. One micromolar RWJ-67657, an inhibitor of p38 MAPK activity, diminished TNF-α- and IL-1β-induced expression of IL-6, IL-8, and E-selectin but had little effect on VCAM-1 and ICAM-1. Combined treatment of HUVEC with MOL-294 and RWJ-67657 resulted in significant blocking of the expression of E-selectin, IL-6, IL-8, and COX-2. The inhibitory effects were much stronger than those observed with single drug treatment. Application of combinations of drugs that affect multiple targets in activated endothelial cells may therefore be considered as a potential new therapeutic strategy to inhibit inflammatory disease activity.

Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein (MRP) affects transmigration in activated RAW264.7 cells

2009 ◽  
Vol 256 (1-2) ◽  
pp. 92-98 ◽  
Author(s):  
Kwang-Rok Chun ◽  
Eun Mi Bae ◽  
Jae-Kwan Kim ◽  
Kyoungho Suk ◽  
Won-Ha Lee
Keyword(s):  
Raw264.7 Cells ◽  
Related Protein ◽  
Induced Expression

scholarly journals Resveratrol Reduces the Proinflammatory Effects and Lipopolysaccharide- Induced Expression of HMGB1 and TLR4 in RAW264.7 Cells

2014 ◽  
Vol 33 (5) ◽  
pp. 1283-1292 ◽  
Author(s):  
Ying Yang ◽  
Shuping Li ◽  
Qiao Yang ◽  
Yu Shi ◽  
Min Zheng ◽  
...  
Keyword(s):  
Raw264.7 Cells ◽  
Induced Expression
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