Inhibitory Effect of Litchi (Litchi chinensis Sonn.) Flower on Lipopolysaccharide-Induced Expression of Proinflammatory Mediators in RAW264.7 Cells through NF-κB, ERK, and JAK2/STAT3 Inactivation

2014 ◽  
Vol 62 (15) ◽  
pp. 3458-3465 ◽  
Author(s):  
Deng-Jye Yang ◽  
Yuan-Yen Chang ◽  
Hui-Wen Lin ◽  
Yi-Chen Chen ◽  
Shih-Han Hsu ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Raw264.7 Cells ◽  
Induced Expression ◽  
Litchi Chinensis ◽  
Proinflammatory Mediators

scholarly journals The Inhibitory Effect of a Novel Polypeptide Fraction fromArca subcrenataon Cancer-Related Inflammation in Human Cervical Cancer HeLa Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Wu ◽  
Xianjing Hu ◽  
Liyan Song ◽  
Jianhua Zhu ◽  
Rongmin Yu
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Molecular Mechanisms ◽  
Tumor Development ◽  
Basic Research ◽  
Inhibitory Effect ◽  
Fishery Resource ◽  
Proinflammatory Mediators ◽  
Raw264.7 Macrophage ◽  
Human Cervical Cancer

Inflammation is known to be closely associated with the development of cancer. The study was launched in human cervical cancer HeLa cells to investigate the antitumor and anti-inflammatory effects of P2, a marine polypeptide fraction from an important fishery resourceArca subcrenata. The basic research showed that P2 could suppress the production of nitric oxide in LPS-induced RAW264.7 macrophage cells as well as the secretion of inflammatory cytokines IL-6 and TNF-αin human cervical cancer HeLa cells. For the molecular mechanisms, P2 was shown to downregulate the gene expression of proinflammatory cytokines IL-6 and IL-8 and to inhibit the COX-2 and iNOS-related pathways in HeLa cells. In consequence, P2 might inhibit tumor development by blocking the interaction between tumor microenvironment and proinflammatory mediators. All findings indicate that P2 possesses the potential to be developed as a novel agent for cancer therapy.

Sageretia thea Inhibits Inflammation through Suppression of NF-κB and MAPK and Activation of Nrf2/HO-1 Signaling Pathways in RAW264.7 Cells

2019 ◽  
Vol 47 (02) ◽  
pp. 385-403 ◽  
Author(s):  
Ha Na Kim ◽  
Gwang Hun Park ◽  
Su Bin Park ◽  
Jeong Dong Kim ◽  
Hyun Ji Eo ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Mapk Signaling ◽  
Inflammatory Activity ◽  
Nuclear Accumulation ◽  
Raw264.7 Cells ◽  
No Production ◽  
Immunodeficiency Virus ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Signaling Activation

Sageretia thea (S. thea) commonly known as Chinese sweet plum or Chinese bird plum has been used for treating hepatitis and fevers in Korea and China. S. thea has been reported to exert anti-oxidant, anticancer and anti-human immunodeficiency virus activity. However, there is little study on the anti-inflammatory activity of S. thea. Thus, we evaluated the anti-inflammatory effect of extracts of leaves (ST-L) and branches (ST-B) from Sageretia thea in LPS-stimulated RAW264.7 cells. ST-L and ST-B significantly inhibited the production of the pro-inflammatory mediators such as NO, iNOS, COX-2, IL-1[Formula: see text] and IL-6 in LPS-stimulated RAW264.7 cells. ST-L and ST-B blocked LPS-induced degradation of I[Formula: see text]B-[Formula: see text] and nuclear accumulation of p65, which resulted in the inhibition of NF-[Formula: see text]B activation in RAW264.7 cells. ST-L and ST-B also attenuated the phosphorylation of ERK1/2, p38 and JNK in LPS-stimulated RAW264.7 cells. In addition, ST-L and ST-B increased HO-1 expression in RAW264.7 cells, and the inhibition of HO-1 by ZnPP reduced the inhibitory effect of ST-L and ST-B against LPS-induced NO production in RAW264.7 cells. Inhibition of p38 activation and ROS elimination attenuated HO-1 expression by ST-L and ST-B, and ROS elimination inhibited p38 activation induced by ST-L and ST-B. ST-L and ST-B dramatically induced nuclear accumulation of Nrf2, but this was significantly reversed by the inhibition of p38 activation and ROS elimination. Collectively, our results suggest that ST-L and ST-B exerts potential anti-inflammatory activity by suppressing NF-[Formula: see text]B and MAPK signaling activation, and activating HO-1 expression through the nuclear accumulation of Nrf2 via ROS-dependent p38 activation. These findings suggest that ST-L and ST-B may have great potential for the development of anti-inflammatory drug to treat acute and chronic inflammatory disorders.

scholarly journals Anti-Inflammatory Effects of Essential Oils of Amomum aromaticum Fruits in Lipopolysaccharide-Stimulated RAW264.7 Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-5 ◽  
Author(s):  
Nguyen Hai Dang ◽  
Le Thi Van Anh ◽  
Nguyen Tien Dat
Keyword(s):  
Essential Oils ◽  
Chronic Inflammation ◽  
Raw264.7 Cells ◽  
Phytochemical Analysis ◽  
Cellular Injury ◽  
Volatile Oils ◽  
Physiologic Response ◽  
Proinflammatory Mediators ◽  
Anti Inflammatory ◽  
First Time

Inflammation is a vital physiologic response of cellular injury, infection, or autoimmune activation. Overproduction of proinflammatory mediators may result in the chronic inflammation that leads to many diseases such as rheumatoid arthritis, asthma, multiple sclerosis, and atherosclerosis. In this study, we assessed for the first time the anti-inflammatory effects of the essential oils of Amomum aromaticum fruits (AAE) in RAW264.7 murine macrophage model. As a result, AAE potently inhibited the production of nitric oxide in LPS-induced RAW264.7 cells with the IC50 value of 0.45 ± 0.11 μg/ml. AAE also dose-dependently reduced the expression of two proinflammatory proteins iNOS and COX-2 in the stimulated cells. Phytochemical analysis revealed that major compositions of the volatile oils including 1,8 cineole (48.22%), geranial (9.24%), neral (6.72%), α-pinene (2.43%), and α-terpineol (2.28%) may contribute greatly to the inhibition effects due to their anti-inflammatory properties. The results suggest for the potential uses of AAE in chronic inflammation prevention.

Inhibitory effect of activin A on activation of lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells

Cytokine ◽  
2008 ◽  
Vol 42 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Shi-Yao Wang ◽  
Gui-Xiang Tai ◽  
Peng-Yu Zhang ◽  
Da-Peng Mu ◽  
Xue-Jun Zhang ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Activin A ◽  
Raw264.7 Cells ◽  
Mouse Macrophage

scholarly journals Leptomeningeal Cells Transduce Peripheral Macrophages Inflammatory Signal to Microglia in Reponse toPorphyromonas gingivalisLPS

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Yicong Liu ◽  
Zhou Wu ◽  
Xinwen Zhang ◽  
Junjun Ni ◽  
Weixian Yu ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Chronic Periodontitis ◽  
Human Monocyte ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Proinflammatory Mediators ◽  
Raw264.7 Macrophages ◽  
Inflammatory Signals ◽  
The Mean

We report here that the leptomeningeal cells transduce inflammatory signals from peripheral macrophages to brain-resident microglia in response toPorphyromonas gingivalis (P.g.)LPS. The expression of Toll-like receptor 2 (TLR2), TLR4, TNF-α, and inducible NO synthase was mainly detected in the gingival macrophages of chronic periodontitis patients. Inin vitrostudies,P.g.LPS induced the secretion of TNF-αand IL-1βfrom THP-1 human monocyte-like cell line and RAW264.7 mouse macrophages. Surprisingly, the mean mRNA levels of TNF-αand IL-1βin leptomeningeal cells after treatment with the conditioned medium fromP.g.LPS-stimulated RAW264.7 macrophages were significantly higher than those after treatment withP.g.LPS alone. Furthermore, the mean mRNA levels of TNF-αand IL-1βin microglia after treatment with the conditioned medium fromP.g.LPS-stimulated leptomeningeal cells were significantly higher than those afterP.g.LPS alone. These observations suggest that leptomeninges serve as an important route for transducing inflammatory signals from macrophages to microglia by secretion of proinflammatory mediators during chronic periodontitis. Moreover, propolis significantly reduced theP.g.LPS-induced TNF-αand IL-1βproduction by leptomeningeal cells through inhibiting the nuclear factor-κB signaling pathway. Together with the inhibitory effect on microglial activation, propolis may be beneficial in preventing neuroinflammation during chronic periodontitis.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Phenolic-Enriched Extracts ofSmilax glabra

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Chuan-li Lu ◽  
Wei Zhu ◽  
Min Wang ◽  
Xiao-jie Xu ◽  
Chuan-jian Lu
Keyword(s):  
Ascorbic Acid ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ultrahigh Pressure ◽  
Raw264.7 Cells ◽  
Suppression Effect ◽  
Proinflammatory Mediators ◽  
Significant Difference ◽  
Anti Inflammatory

Smilax glabraRoxb. has been used for a long time as both food and folk medicine. In the present study, phenolic-enriched extract ofS. glabra(PEESG) was extracted with 70% ethanol and purified by HP-20 column chromatography. Its antioxidant and anti-inflammatory activities were evaluated by radical scavenging assay, reducing power determination, and lipopolysaccharide (LPS)-induced RAW264.7 cells assays, respectively. PEESG exhibited obviously scavenging capacity for DPPH and ABTS radicals, as well as significant reducing power for ferric ion. Particularly, PEESG (12.5–50 μg/mL) showed a significantly higher efficiency for scavenging ABTS than that of ascorbic acid and no significant difference with ascorbic acid for DPPH scavenging. PEESG also possessed a significant suppression effect on proinflammatory mediators production, such as nitric oxide (NO), tumor necrosis factor-α(TNF-α), and interleukin-6 (IL-6), in LPS-induced RAW264.7 cells. In addition, the main ingredients of PEESG were identified using ultrahigh pressure liquid chromatography coupled to electrospray mass spectrometry (U-HPLC-ESI-MS). Seventeen components, including 5-O-caffeoylshikimic acid, neoastilbin, astilbin, neoisoastilbin, isoastilbin, engetin and isoengeletin were identified. These findings strongly suggest the potential of PEESG as a natural antioxidant and anti-inflammatory agent.

Effect of curcumin on acidic pH-induced expression of IL-6 and IL-8 in human esophageal epithelial cells (HET-1A): role of PKC, MAPKs, and NF-κB

2009 ◽  
Vol 296 (2) ◽  
pp. G388-G398 ◽  
Author(s):  
Parvaneh Rafiee ◽  
Victoria M. Nelson ◽  
Sharon Manley ◽  
Michael Wellner ◽  
Martin Floer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mucosal Injury ◽  
Inhibitory Effect ◽  
Acidic Ph ◽  
Induced Expression ◽  
Potent Inducer ◽  
Mrna And Protein Expression ◽  
Esophageal Epithelial Cells ◽  
P38 Mapk Inhibitor

Human esophageal epithelial cells play a key role in esophageal inflammation in response to acidic pH during gastroesophageal reflux disease (GERD), increasing secretion of IL-6 and IL-8. The mechanisms underlying IL-6 and IL-8 expression and secretion in esophageal epithelial cells after acid stimulation are not well characterized. We investigated the role of PKC, MAPK, and NF-κB signaling pathways and transcriptional regulation of IL-6 and IL-8 expression in HET-1A cells exposed to acid. Exposure of HET-1A cells to pH 4.5 induced NF-κB activity and enhanced IL-6 and IL-8 secretion and mRNA and protein expression. Acid stimulation of HET-1A cells also resulted in activation of MAPKs and PKC (α and ε). Curcumin, as well as inhibitors of NF-κB (SN-50), PKC (chelerythrine), and p44/42 MAPK (PD-098059) abolished the acid-induced expression of IL-6 and IL-8. The JNK inhibitor SP-600125 blocked expression/secretion of IL-6 but only partially attenuated IL-8 expression. The p38 MAPK inhibitor SB-203580 did not inhibit IL-6 expression but exerted a stronger inhibitory effect on IL-8 expression. Together, these data demonstrate that 1) acid is a potent inducer of IL-6 and IL-8 production in HET-1A cells; 2) MAPK and PKC signaling play a key regulatory role in acid-mediated IL-6 and IL-8 expression via NF-κB activation; and 3) the anti-inflammatory plant compound curcumin inhibits esophageal activation in response to acid. Thus IL-6 and IL-8 expression by acid may contribute to the pathobiology of mucosal injury in GERD, and inhibition of the NF-κB/proinflammatory cytokine pathways may emerge as important therapeutic targets for treatment of esophageal inflammation.

Inhibitory effect of citral on NO production by suppression of iNOS expression and NF-κB activation in RAW264.7 cells

2008 ◽  
Vol 31 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Hwa Jeong Lee ◽  
Heon Sang Jeong ◽  
Dae Joong Kim ◽  
Young Hee Noh ◽  
Dong Yeon Yuk ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Inos Expression ◽  
Raw264.7 Cells ◽  
No Production

scholarly journals Suppressive Effects of Alloxanthin and Diatoxanthin from Halocynthia roretzi on LPS-induced Expression of Pro-inflammatory Genes in RAW264.7 Cells

2008 ◽  
Vol 57 (3) ◽  
pp. 181-189 ◽  
Author(s):  
Izumi Konishi ◽  
Masashi Hosokawa ◽  
Tokutake Sashima ◽  
Takashi Maoka ◽  
Kazuo Miyashita
Keyword(s):  
Raw264.7 Cells ◽  
Halocynthia Roretzi ◽  
Induced Expression ◽  
Inflammatory Genes
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