scholarly journals The potential teratogenic effects of interferon beta-1a and interferon beta-1b on in vitro embryonic development

2016 ◽  
Vol 75 (2) ◽  
pp. 257-263 ◽  
Author(s):  
İ. Uçar ◽  
T. Ertekin ◽  
M. Nisari ◽  
D. Ceylan ◽  
Ö. Al ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Interferon Beta ◽  
Teratogenic Effects ◽  
In Vitro Embryonic Development

scholarly journals Hydrosalpingeal fluid inhibits in-vitro embryonic development in a murine model

1997 ◽  
Vol 12 (12) ◽  
pp. 2724-2728 ◽  
Author(s):  
S. A. Beyler ◽  
K. P. James ◽  
M. A. Fritz ◽  
W. R. Meyer
Keyword(s):  
Embryonic Development ◽  
Murine Model ◽  
In Vitro Embryonic Development

137 Kinetics of In Vitro Embryonic Development According to the Follicular Population in Bos Indicus

2018 ◽  
Vol 30 (1) ◽  
pp. 208
Author(s):  
J. G. Soares ◽  
F. M. Morato ◽  
G. F. Rossi ◽  
B. M. Bayeux ◽  
A. S. Oliveira ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Formation Rate ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Lower Percentage ◽  
Embryo Production ◽  
High Category ◽  
Kinetics Of ◽  
In Vitro Embryonic Development

The present study aimed to evaluate the effect of the follicular population from cull Nelore (Bos indicus) on the kinetics of in vitro embryonic development. At random stages of the oestrous cycle (Day –5), a total of 28 cull Nelore cows were synchronized with an intravaginal progesterone device associated with oestradiol benzoate (2.0 mg IM). At the same moment, a dose of prostaglandin F2α (2.0 mg im) was also administered to promote luteolysis and absence of corpus luteum (CL) at the time of ovum pick-up (OPU). Five days later (Day 0), all cows underwent an OPU session and the recovered oocytes were submitted to in vitro embryo production (IVEP). The same procedures were repeated 2 times at 30-day intervals (Day 25 and Day 55). Semen from a single batch of a previously tested bull was used for all IVEP. Blastocyst production and hatching were verified on Days 7, 8, and 9 of the IVEP. Data were analysed by the GLIMMIX procedure of SAS 9.3® (SAS Institute Inc., Cary, NC, USA). Data of the 3 OPU sessions were grouped and the cows were classified into 3 categories according to the follicular population: Low (19.7 ± 0.9 follicles, n = 27), Medium (33.5 ± 0.8 follicles, n = 29), and High (58.7 ± 3.2 follicles, n = 28). The Low category had a lower rate of viable oocytes [(number of viable oocytes/total number of oocytes) × 100; 62.0 v. 69.5%; P = 0.02] and cleavage rate [(number of cleaved/total number of oocytes) × 100; 55.9 v. 66.8%; P = 0.001] than the High category. The blastocyst formation rate [number of blastocysts/total number of oocytes) × 100] on Day 7 and Day 8 was lower for Low and Medium compared with the High category (Day 7: 26.1b v. 29.0b v. 35.1a %; P = 0.001; and Day 8: 29.2b v. 30.2b v. 34.7a %; P = 0.05). No differences were found in blastocysts rate on Day 9 among Low, Medium, and High categories (14.1 v. 15.9 v. 16.2%; P = 0.61). However, Low category had a lower percentage of hatched blastocysts [(number of hatched blastocysts/number of blastocysts) × 100] on Day 7 compared with High category (2.9 v. 12.0%; P = 0.01). These results reported that cull Nelore with High follicular population showed higher rates of embryo production and hatched blastocysts compared with cows with Low follicular population. We concluded that the kinetics of in vitro embryonic development was compromised in cull Nelore (Bos indicus) with low follicular population submitted to OPU-IVEP. This research was supported by Fapesp 2012/50533–2 (GIFT).

Metabolism, protein content, and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose andL-lactate

2006 ◽  
Vol 73 (2) ◽  
pp. 256-266 ◽  
Author(s):  
Jason R. Herrick ◽  
Michelle Lane ◽  
David K. Gardner ◽  
Esmail Behboodi ◽  
Erdogan Memili ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Protein Content ◽  
In Vitro Embryonic Development

Effect of sperm pretreatment with sodium hydroxide and dithiothreitol on the efficiency of bovine intracytoplasmic sperm injection

2014 ◽  
Vol 26 (6) ◽  
pp. 847 ◽  
Author(s):  
M. E. Arias ◽  
R. Sánchez ◽  
J. Risopatrón ◽  
L. Pérez ◽  
R. Felmer
Keyword(s):  
Embryonic Development ◽  
Intracytoplasmic Sperm Injection ◽  
Membrane Integrity ◽  
Control Group ◽  
Developmental Potential ◽  
Bovine Spermatozoa ◽  
Pronuclear Formation ◽  
First Time ◽  
In Vitro Embryonic Development

The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is lower than in other species due, in part, to a lack of optimal conditions for its implementation; this has hindered the achievement of high rates of embryonic development and the birth of live offspring. The aim of the present study was to evaluate the effects of pretreatment of bovine spermatozoa with NaOH and dithiothreitol (DTT) on the viability, plasma membrane integrity, DNA fragmentation and in vitro developmental potential of embryos generated by ICSI. Following pretreatment of spermatozoa with 5 mM DTT for 20 min and a low concentration of NaOH (1 mM for 60 min), there were fewer live and acrosome reacted spermatozoa (44% and 34%, respectively) than in the control group without treatment (82%). Spermatozoa subjected to higher alkali concentrations (10–50 mM) were mostly dead and reacted. However, pronuclear formation, cleavage, blastocyst rate and embryo quality did not differ between these pretreatment groups and the untreated control group. In conclusion, we have described, for the first time, the effects of NaOH treatment on bovine spermatozoa and subsequent in vitro embryonic development after ICSI, and have demonstrated that pretreatment of bovine spermatozoa with NaOH or DTT is not necessary for an appropriate in vitro embryo development in this species.

Effect of visual light on in vitro embryonic development in the hamster

1992 ◽  
Vol 38 (6) ◽  
pp. 1043-1054 ◽  
Author(s):  
Y. Umaoka ◽  
Y. Noda ◽  
T. Nakayama ◽  
K. Narimoto ◽  
T. Mori ◽  
...  
Keyword(s):  
Embryonic Development ◽  
In Vitro Embryonic Development ◽  
Visual Light

scholarly journals Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe diffusa and embryotoxicity assessment in a chicken embryo model

2021 ◽  
Vol 65 (1) ◽  
pp. 47-57
Author(s):  
Mycheli P. da Cruz ◽  
Lucas T. Larentis ◽  
Edgar de S. Vismara ◽  
Lilian de S. Vismara ◽  
Patricia F. de Freitas ◽  
...  
Keyword(s):  
Heart Rate ◽  
Embryonic Development ◽  
Ganoderma Lucidum ◽  
Mycelial Growth ◽  
Antimicrobial Effect ◽  
Teratogenic Effects ◽  
Embryo Survival ◽  
Air Chamber ◽  
Microdilution Broth

This work aimed to evaluate the antimicrobial effect of Ganoderma lucidum mycelial growth filtrates (MGF) on the phytopathogen Erysiphe diffusa and their potential effects on the embryonic development of Gallus gallus. The antimicrobial activity was evaluated on E. diffusa spores by the microdilution broth method. To evaluate embryotoxic and teratogenic effects, fertile eggs of G. gallus received injections of solutions containing the filtrates of G. lucidum through the air chamber. After three days of incubation, we opened the eggs and evaluated egg viability, embryo survival, malformation occurrence, embryonic staging and heart rate. Live embryos were prepared using whole mount technique and the morphological analysis was performed. We used the generalized linear model to fit embryotoxicity and teratogenicity data. We verified that G. lucidum MGF showed inhibitory activity in vitro against E. diffusa and the minimum inhibitory concentrations ranged from 5 to 10 mg/mL. We could also observe that the filtrates did not present embryotoxic or teratogenic effects on the early embryonic development of G. gallus, but induced significant differences in the embryonic mean heart rate and on the stage of embryonic development.

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