scholarly journals Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

2016 ◽  
Vol 85 (2) ◽  
Author(s):  
Saranjeet Kaur ◽  
Kamlesh Kumar Bhutani
Keyword(s):  
Seed Germination ◽  
Shoot Multiplication ◽  
Ex Situ ◽  
Terrestrial Orchid ◽  
Asymbiotic Seed Germination ◽  
Orchid Seed ◽  
Early Seedling ◽  
Potato Powder ◽  
Survival Frequency

The present study was intended to facilitate ex situ conservation of <em>Paphiopedilum venustum</em>, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving maximum seed germination and early seedling development. The effect of darkness and 12-h photoperiod was also tested. Optimum seed germination, i.e., 82.7% was achieved on modified terrestrial orchid medium under a 12-h photoperiod using seeds from undehisced capsules. Shoot multiplication was accomplished using organic [peptone (1.0, 2.0 g L<sup>−1</sup>)] and inorganic [banana homogenate (10, 20, 30 g L<sup>−1</sup>) and potato powder (5.0, 10 g L<sup>−1</sup>)] growth supplements. Peptone at 1.0 g L<sup>−1</sup> was the most effective in multiplying the shoots. Plantlets were acclimatized in the greenhouse with 80% survival frequency.

Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea

2008 ◽  
Vol 94 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Daniela Dutra ◽  
Timothy R. Johnson ◽  
Philip J. Kauth ◽  
Scott L. Stewart ◽  
Michael E. Kane ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Development ◽  
Terrestrial Orchid ◽  
Asymbiotic Seed Germination

scholarly journals Influence of Photoperiod and Culture Medium on the Speed of Asymbiotic Seed Germination and Seedling Development in Spathoglottis plicata

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1570-1575
Author(s):  
Boris Andrés Bran Barrientos ◽  
Jong-Yi Fang
Keyword(s):  
Seed Germination ◽  
Culture Medium ◽  
Developmental Stages ◽  
Culture Media ◽  
Seedling Development ◽  
Advanced Stage ◽  
Terrestrial Orchid ◽  
Asymbiotic Seed Germination ◽  
Orchid Seed ◽  
Half Strength

Spathoglottis plicata is an attractive, easy-to-grow, and floriferous terrestrial orchid that has become the most important horticulturally cultivated Spathoglottis species in Southeast Asia. The present research was conducted to study the asymbiotic seed germination and seedling development of this orchid under the influence of various photoperiod and medium treatments. Seeds from 28-day-old capsule were sown on five culture media, including half-strength Murashige and Skoog medium (1/2 MS), Orchid Seed Sowing Medium (OSSM), BM-1 Terrestrial Orchid Medium (BM-1), Vacin and Went Modified Orchid Medium (VW), and Knudson C Orchid Medium (KC), and incubated under 0/24-hour or 16/8-hour light/dark photoperiod. Seed germination occurred in all photoperiods and media tested but at different paces. Seeds subjected to total darkness germinated more rapidly in the first 3 weeks than those subjected to light. However, seed germination under light overtook or even exceeded seed germination under dark starting on the fourth week of culture. Seedlings grown on the OSSM and VW media showed the fastest development as they reached the advanced stage (Stage 6) within 11 weeks of culture. Seedlings on the BM-1 medium were the slowest to evolve, as they required more than 16 weeks’ time to complete all the developmental stages. Light-incubated advanced stage seedlings were subcultured on the same medium until leaves and roots were well developed and acclimatized in the greenhouse with 100% survival.

scholarly journals In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Growth Regulators ◽  
Subsequent Development ◽  
Ms Medium ◽  
Asymbiotic Seed Germination ◽  
Endangered Orchid ◽  
Maximum Root ◽  
Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   

scholarly journals Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
DWI MURTI PUSPITANINGTYAS ◽  
ELIZABETH HANDINI
Keyword(s):  
Regression Analysis ◽  
Seed Germination ◽  
Life Span ◽  
Seed Storage ◽  
Optimum Conditions ◽  
Presidential Decree ◽  
Orchid Seed ◽  
Leaf Fertilizer

Abstract. Puspitaningtyas DM, Handini E. 2021. Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation. Biodiversitas 22: 5231-5238. Phalaenopsis amabilis (L.) Blume (moon orchid) is one of Indonesia's national flowers, which is mentioned in Presidential Decree No. 4/1993. Study on the orchid seed of P. amabilis was conducted to observe the longevity of seeds storage. The purpose of this study was to determine the viability of P. amabilis seeds after storage for years, then to predict the life span of the seeds, and to find out the best media germination for P. amabilis seeds. After harvesting, P. amabilis seeds were stored at –20 °C and the germination tests were carried out periodically in different periods until the seeds lose their viability to determine the optimum conditions for long-term seed storage. Four trial media cultures have been used to test seed germination by means of in vitro culture. The result showed that seeds of P. amabilis were able to germinate in 4 media i.e. Knudson C, modified Knudson C, modified Vacin & Went and modified leaf fertilizer. The best germination was on leaf fertilizer medium. Based on the research showed that the viability seeds of P. amabilis decreased sharply after 6 years stored. By using regression analysis, the life span of the seeds could be predicted can reach 9 years of storage which is sowing on the best media (leaf fertilizer).

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

scholarly journals Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Potshangbam Nongdam ◽  
Leimapokpam Tikendra
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Germination Percentage ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Seed Culture ◽  
Leaf Formation

An efficientin vitroregeneration protocol from seed culture has been established successfully forDendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values. Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with 0.4% activated charcoal (AC), 2 mg/L 6-benzyl amino purine (BAP), and 2 mg/L indole-3-acetic acid (IAA) produced best seed germination percentage in 2 weeks of culture. Incorporation of higher concentration of kinetin (KN) or BAP in combination with low auxin in medium induced pronounced shooting and leaf formation. Reduction in leaf development was evident when cytokinins exist singly in medium indicating synergistic effect of auxin and cytokinin in leaf induction. Presence of elevated level of indole-3-butyric acid (IBA) or 1-naphthalene acetic acid (NAA) with low cytokinin content in medium generated morein vitrorooting, though IBA was found to be more effective in rooting induction as compared to NAA. Thein vitroprotocol for asymbiotic seed germination developed from the present investigation can be used for rapid mass propagation of this highly importantDendrobiumorchid species.

scholarly journals Asymbiotic in vitro germination and seed quality assessment of Australian terrestrial orchids

2012 ◽  
Vol 60 (7) ◽  
pp. 592 ◽  
Author(s):  
Nicole Dowling ◽  
Manfred Jusaitis
Keyword(s):  
Seed Quality ◽  
Culture Media ◽  
Vital Staining ◽  
Terrestrial Orchid ◽  
Triphenyltetrazolium Chloride ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Terrestrial Orchids ◽  
Asymbiotic Germination

Determining the seed quality and germination requirements for threatened orchid species in storage is vital for future conservation efforts. Seeds of many Australian terrestrial orchid species are held in conservation collections around the country, but few have been germinated in vitro, fuelling concerns over their long-term viability. This study tested three methods of assessing orchid seed quality; asymbiotic germination was compared with vital staining using triphenyltetrazolium chloride or fluorescein diacetate. Six culture media were examined for efficacy in promoting asymbiotic seed germination of four Australian terrestrial orchid species (Pterostylis nutans, Microtis arenaria, Thelymitra pauciflora and Prasophyllum pruinosum). Germination occurred on all media but germination rates were consistently highest on BM1 and development was most advanced on BM1, P723 and Malmgren media. Subsequent trials tested the efficacy of BM1 for asymbiotic germination of additional genera (Caladenia, Calochilus and Diuris), several congeneric species, and two species collected from several different provenances within each of their ranges. The results indicate that asymbiotic germination on BM1 medium is an effective technique for testing the performance of Australian terrestrial orchid seeds. The efficacy of vital stains to determine seed viability, however, remains uncertain, as significant disagreement between degree of staining and germinability was observed for some species.

Sign in / Sign up

Export Citation Format

Share Document