scholarly journals Pilosella bauhinii (Schult.) Arv.-Touv. and P. cymosa subsp. vaillantii (Tausch) S. Bräut. & Greuter (Asteraceae) from new localities in north-central Poland

2015 ◽  
Vol 84 (4) ◽  
pp. 449-451 ◽  
Author(s):  
Radosław Puchałka ◽  
Dominika Wyborska ◽  
Lucjan Rutkowski ◽  
Marcin Piwczyński
Keyword(s):  
Intergenic Spacer ◽  
Pairwise Comparison ◽  
Original Description ◽  
Abundant Species ◽  
Reference Database ◽  
Unique Haplotype ◽  
North Central ◽  
Additional Information ◽  
Central Poland ◽  
New Localities

We provide information about two species, <em>Pilosella bauhinii</em> and <em>P. cymosa</em> subsp. <em>vaillantii</em> (Asteraceae), from new localities in north-central Poland (ATPOL DC18). All individuals of both species grew on a railway embankment in Górzno-Lidzbark Landscape Park. The ecological preferences for <em>P. cymosa</em> and <em>P. bauhinii</em> estimated according to average Ellenberg values nearly coincided with the original description of Ellenberg. We also sequenced the barcode marker, plastid <em>trnH-psbA</em> intergenic spacer, for two individuals of <em>P. bauhinii</em>, three specimens of <em>P. cymosa</em> subsp. <em>vaillantii</em>, and additionally two individuals of <em>P. officinarum</em> L. and one <em>H. murorum</em> L. growing in close proximity. A pairwise comparison of <em>trnH-psbA</em> sequences showed that each species has a unique haplotype. Taking into account their morphological coherence, it is possible that both <em>P. bauhinii</em> and <em>P. cymosa</em> have not hybridized yet, at least locally, with the more abundant species (e.g., <em>P. officinarum</em>). A search for reference sequences did not provide additional information because of the low quality of the reference database for this group in GenBank. Only 14 sequences of <em>trnH-psbA</em> were available with some apparently being misidentified or of low quality. None were identical to sequences of <em>P. cymosa</em> subsp. <em>vaillantii</em> and <em>P. bauhinii</em> found in this study.

scholarly journals A Universal Approach to Molecular Identification of Rumen Fluke Species Across Hosts, Continents, and Sample Types

2021 ◽  
Vol 7 ◽  
Author(s):  
Gillian Mitchell ◽  
Ruth N. Zadoks ◽  
Philip J. Skuce
Keyword(s):  
Species Identification ◽  
Molecular Identification ◽  
Sequence Data ◽  
Intergenic Spacer ◽  
Pairwise Comparison ◽  
Reference Database ◽  
Life Cycle Stages ◽  
Wide Range ◽  
Universal Approach ◽  
Species Comparisons

Rumen fluke are parasitic trematodes that affect domestic and wild ruminants across a wide range of countries and habitats. There are 6 major genera of rumen fluke and over 70 recognized species. Accurate species identification is important to investigate the epidemiology, pathophysiology and economic impact of rumen fluke species but paramphistomes are morphologically plastic, which has resulted in numerous instances of misclassification. Here, we present a universal approach to molecular identification of rumen fluke species, including different life-cycle stages (eggs, juvenile and mature fluke) and sample preservation methods (fresh, ethanol- or formalin-fixed, and paraffin wax-embedded). Among 387 specimens from 173 animals belonging to 10 host species and originating from 14 countries on 5 continents, 10 rumen fluke species were identified based on ITS-2 intergenic spacer sequencing, including members of the genera Calicophoron, Cotylophoron, Fischeroedius, Gastrothylax, Orthocoelium, and Paramphistomum. Pairwise comparison of ITS-2 sequences from this study and GenBank showed &gt;98.5% homology for 80% of intra-species comparisons and &lt;98.5% homology for 97% of inter-species comparisons, suggesting that some sequence data may have been entered into public repositories with incorrect species attribution based on morphological analysis. We propose that ITS-2 sequencing could be used as a universal tool for rumen fluke identification across host and parasite species from diverse technical and geographical origins and form the basis of an international reference database for accurate species identification.

Stromatoporoids from the Famennian (Devonian) Wabamun Formation, Normandville oilfield, north–central Alberta, Canada

1988 ◽  
Vol 62 (03) ◽  
pp. 411-419 ◽  
Author(s):  
Colin W. Stearn
Keyword(s):  
Patch Reef ◽  
Abundant Species ◽  
The Other ◽  
North Central ◽  
Biotic Crisis

Stromatoporoids are the principal framebuilding organisms in the patch reef that is part of the reservoir of the Normandville field. The reef is 10 m thick and 1.5 km2in area and demonstrates that stromatoporoids retained their ability to build reefal edifices into Famennian time despite the biotic crisis at the close of Frasnian time. The fauna is dominated by labechiids but includes three non-labechiid species. The most abundant species isStylostroma sinense(Dong) butLabechia palliseriStearn is also common. Both these species are highly variable and are described in terms of multiple phases that occur in a single skeleton. The other species described areClathrostromacf.C. jukkenseYavorsky,Gerronostromasp. (a columnar species), andStromatoporasp. The fauna belongs in Famennian/Strunian assemblage 2 as defined by Stearn et al. (1988).

Did parity affect bone mineral density of women in past populations? Parturition scars and BMD of Neolithic to modern skeletons from north-central Poland

2020 ◽  
Vol 124 ◽  
pp. 105264
Author(s):  
Wiesław Lorkiewicz ◽  
Justyna Karkus ◽  
Joanna Mietlińska ◽  
Michał Stuss ◽  
Ewa Sewerynek ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Mineral Density ◽  
North Central ◽  
Central Poland

scholarly journals New Localities of Rare Liverworts in the Central Poland

2014 ◽  
Vol 63 (2) ◽  
Author(s):  
Monika Staniaszek-Kik
Keyword(s):  
Peat Bog ◽  
Swamp Forest ◽  
Opencast Mine ◽  
Central Poland ◽  
New Localities ◽  
Depression Cone

Abstract The present study reports new localities of four liverwort species, i.e., Fossombronia foveolata Lindb., Gymnocolea inflata (Huds.) Dumort., Leiomylia anomala (Hook.) J.J. Engel & Braggins and Odontoschisma denudatum (Mart.) Dumort., found in Central Poland. The new sites are situated in peat bog habitats and swamp forest in the depression cone of lignite opencast mine near Bełchatów in Central Poland. All of the reported species are very rare and have only few localities in Central Poland.

scholarly journals Molecular characterization of Aeromonas spp. and Vibrio cholerae O1 isolated during a diarrhea outbreak

2012 ◽  
Vol 54 (6) ◽  
pp. 299-304 ◽  
Author(s):  
Carina Lucena Mendes-Marques ◽  
Larissa Mélo do Nascimento ◽  
Grace Nazareth Diogo Theophilo ◽  
Ernesto Hofer ◽  
Osvaldo Pompílio de Melo Neto ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Intergenic Spacer ◽  
Abundant Species ◽  
Environmental Isolates ◽  
Pathogenic Potential ◽  
Intergenic Spacer Region ◽  
Clonal Relatedness ◽  
Pathogenic Genes ◽  
Vibrio Cholerae O1 ◽  
Definition Of

This work aimed to assess pathogenic potential and clonal relatedness of Aeromonas sp. and Vibrio cholerae isolates recovered during a diarrhea outbreak in Brazil. Clinical and environmental isolates were investigated for the presence of known pathogenic genes and clonal relatedness was assessed by intergenic spacer region (ISR) 16S-23S amplification. Four Aeromonas genes (lip, exu, gcat, flaA/B) were found at high overall frequency in both clinical and environmental isolates although the lip gene was specifically absent from selected species. A fifth gene, aerA, was rarely found in A. caviae, the most abundant species. The ISR profile revealed high heterogeneity among the Aeromonas isolates and no correlation with species identification. In contrast, in all the V. cholerae isolates the four genes investigated (ctxA, tcpA, zot and ace) were amplified and revealed homogeneous ISR and RAPD profiles. Although Aeromonas isolates were the major enteric pathogen recovered, their ISR profiles are not compatible with a unique cause for the diarrhea events, while the clonal relationship clearly implicates V. cholerae in those cases from which it was isolated. These results reinforce the need for a better definition of the role of aeromonads in diarrhea and whether they benefit from co-infection with V. cholerae.

Microperla qinlinga Chen reviewed with supplementary illustrations and the description of the nymph

Zootaxa ◽  
2019 ◽  
Vol 4706 (3) ◽  
pp. 427-438
Author(s):  
QING-BO HUO ◽  
YU-ZHOU DU
Keyword(s):  
Original Description ◽  
Additional Information ◽  
First Time

Microperla qinlinga Chen, 2019 was recently described, but details of the aedeagus were not included in the original description. In this paper, the male aedeagus and nymph of M. qinlinga are described for the first time. Additional information concerning the genus Microperla Chu, 1928 is discussed.

Record of the Vistula ice lobe advances in the Late Weichselian glacial sequence in north-central Poland

2009 ◽  
Vol 207 (1-2) ◽  
pp. 26-41 ◽  
Author(s):  
Wojciech Wysota ◽  
Paweł Molewski ◽  
Robert J. Sokołowski
Keyword(s):  
North Central ◽  
Central Poland ◽  
Late Weichselian

Residual Weeds of Processing Sweet Corn in the North Central Region

2008 ◽  
Vol 22 (4) ◽  
pp. 646-653 ◽  
Author(s):  
Martin M. Williams ◽  
Tom L. Rabaey ◽  
Chris M. Boerboom
Keyword(s):  
Central Region ◽  
Sweet Corn ◽  
Abundant Species ◽  
North Central Region ◽  
Weed Species ◽  
North Central ◽  
Common Lambsquarters ◽  
Weed Density ◽  
Proso Millet ◽  
The North

Knowledge of weed community structure in vegetable crops of the north central region (NCR) is poor. To characterize weed species composition present at harvest (hereafter called residual weeds) in processing sweet corn, 175 fields were surveyed in Illinois, Minnesota, and Wisconsin from 2005 to 2007. Weed density was enumerated by species in thirty 1-m2quadrats placed randomly along a 300- to 500-m loop through the field, and additional species observed outside quadrats were also recorded. Based on weed community composition, population density, and mean plant size, overall weed interference level was rated. A total of 56 residual weed species were observed and no single species dominated the community of NCR processing sweet corn. Several of the most abundant species, such as common lambsquarters and velvetleaf, have been problems for many years, while other species, like wild-proso millet, have become problematic in only the last 20 yr. Compared to a survey of weeds in sweet corn more than 40 yr ago, greater use of herbicides is associated with reductions in weed density by approximately an order of magnitude; however, 57% of fields appeared to suffer yield loss due to weeds. Sweet corn harvest in the NCR ranges from July into early October. Earlier harvests were characterized by some of the highest weed densities, while late-emerging weeds such as eastern black nightshade occurred in fields harvested after August. Fall panicum, giant foxtail, wild-proso millet, common lambsquarters, and velvetleaf were the most abundant species across the NCR, yet each state had some unique dominant weeds.

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