scholarly journals Variation and genetic structure of Serratula lycopifolia populations (Vill.) Kern. (Asteraceae) in Poland and adjacent regions

2013 ◽  
Vol 81 (4) ◽  
pp. 67-75 ◽  
Author(s):  
Elżbieta Cieślak

AFLPs were used to analyze the genetic variability of <em>Serratula lycopifolia</em>, one of the rarest plant species in Central and Western Europe, in six populations from the Wyżyna Małopolska upland (Poland), White Carpathian Mts (Slovakia and Czech Republic) and Podolian Upland (Ukraine). The results of polymorphism, PCoA and neighbor-net analyzes showed similar and relatively low genetic variation and high genetic similarity of individuals within each Polish population but there were differences between those populations. The population from Slovakia also showed comparatively high intrapopulation homogeneity and evident genetic separation from the other studied populations. Intrapopulation genetic variation was higher in the Czech and Ukrainian populations. However, AMOVA analyses revealed no significant differentiation at population and regional levels. The estimated low genetic diversity in the populations from Poland and Slovakia may be due to genetic processes such as genetic drift and inbreeding in local populations resulting from their low abundance, and does not seem characteristic of the species as a whole. These genetic analyzes make it clear that the Polish and Slovakian populations need support programmes to maintain their genetic variation. Measures should focus on increasing the number of individuals in the populations and on protecting their habitat.

2011 ◽  
Vol 60 (1) ◽  
pp. 79-84
Author(s):  
Katarzyna Wąsowicz ◽  
Monika Szczecińska ◽  
Jakub Sawicki

The reasons for low intrapopulation genetic variation in Lamium incisum Willd The paper presents results of a study which aim was preliminary screening of intrapopulation genetic variability in Lamium incisum Willd. This weed species is rarely distributed in Poland and lessening its count during the last years. As a plant inhabiting anthropogenic sites it is exposed to extreme conditions and disturbances caused mostly by the progressive intensification of agriculture. In order to investigate the genetic variability of the selected population markers of ISSR category were used. The analysis of chosen individuals with use of three ISSR primers revealed total of 49 loci, of which only 15 were polymorphic. Nei's gene diversity index (HE=0.099) and the mean number of alleles per locus (AE=1.160) indicated low genetic diversity within the examined population. The research presented in this paper allows for a better learning of the genetic variability of the investigated species and considers probable factors influencing its level.


Author(s):  
Thien Minh Nguyen ◽  
Tien Thi My Pham

The agronomic values of this population have been evaluated in the field experiments based on their phenotypic performance of agronomic traits, but the genetic variability of this population needs to be evaluated via techniques based on genetic material - DNA. In this study, the genetic variability in the investigated population of 71 hybrids and their parents was evaluated by RAPD technique, using eight selected arbitrarily primers; Genetic parameters and dendrogram expressing the genetic relationships among the investigated population were analyzed by GenALEx 6.1, Popgene 1.31 and NTSYSpc 2.1 softwares. Eight primers were used to generate the amplify products on each individual in the investigated population. From 74 genotypes, a total of 109 fragments were generated, among which, there were 89 polymorphic bands representing 81.65% with an average of 11 polymorphic bands/primer. Genetic similarity coefficient among the investigated population, based on DICE coefficient, ranged from 0.560 (LH05/0822 and PB260) to 0.991 (LH05/0781 and LH05/0841) with an average of 0,796, meaning that the genetic distance among ranged from 0.009 to 0.440 with an average of 0.231. The Shannon index and mean heterozygosity values were 0.328 and 0,176, respectively. This indicated that the progenies of the two investigated crosses possessed a relatively high range of genetic variability. The analysis of molecular variance (AMOVA) showed that genetic variation within population represented 62%, while genetic variation among two different crosses contributes 38% to the total genetic variability. Dendrogram based on DICE’s genetic similarity using UPGMA method showed that the hybrids divide into two major genetic groups (0.75), but the crosses were scattered independently of the hybrid.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hedvig Csapó ◽  
Paula Krzywoźniak ◽  
Michał Grabowski ◽  
Remi Wattier ◽  
Karolina Bącela-Spychalska ◽  
...  

Abstract Gammarus roeselii Gervais, 1835 is a morphospecies with a wide distribution range in Europe. The Balkan Peninsula is known as an area of pre-Pleistocene cryptic diversification within this taxon, resulting in at least 13 Molecular Operational Taxonomic Units (MOTUs). The morphospecies diversified there during Neogene and has probably invaded other parts of the continent very recently, in postglacial or even historical times. Thus, the detailed goals of our study were to (1) identify which lineage(s) colonized Central-Western Europe (CWE), (2) determine their possible geographical origin, (3) verify, whether the colonisation was associated with demographic changes. In total, 663 individuals were sequenced for the cytochrome oxidase I (COI) barcoding fragment and 137 individuals for the internal transcribed spacer II (ITS2). We identified two MOTUs in the study area with contrasting Barcode Index Number and haplotype diversities. The Pannonian Basin (PB) appeared to be a potential ice age refugium for the species, while CWE was colonised by a single lineage (also present in PB), displaying low genetic diversity. Our results suggest that G. roeselii is a relatively recent coloniser in CWE, starting demographic expansion around 10 kya.


Nova Hedwigia ◽  
2020 ◽  
Vol 110 (1) ◽  
pp. 79-89
Author(s):  
Vincent Hugonnot ◽  
Leica Chavoutier

The rare Fissidens jansenii Sérgio & Pursell is reported on plateau de Millevaches, Limousin (France), nearly 1000 km north of the nearest known population. As a result, F. jansenii is known from the Iberian Peninsula and now from western France. An updated distribution map of the species in Europe is provided. The new material is fully described and illustrated and the ecology in France is outlined. Gametophytically, French specimens of F. jansenii do not deviate significantly from Portuguese material and the variability of characters is discussed. In France, on plateau de Millevaches, F. jansenii is relatively frequent but constitutes rather small populations, with a low number of individuals. Sporophytes were regularly encountered and reproduction is most likely to be by spore dispersal. On plateau de Millevaches, F. pusillus (Wilson) Milde shares a comparable habitat but is easily distinguished from F. jansenii. Morphologically similar species occurring in Western Europe and that could potentially be confused with F. jansenii (F. rivularis and F. rufulus Bruch & Schimp.) are compared.


2006 ◽  
Vol 159 (2-3) ◽  
pp. 241-243 ◽  
Author(s):  
Ireneusz Soltyszewski ◽  
Magdalena Spolnicka ◽  
Ewa Kartasinska ◽  
Magda Konarzewska ◽  
Witold Pepinski ◽  
...  

2005 ◽  
Vol 53 (8) ◽  
pp. 781 ◽  
Author(s):  
Mayra S. Caldiz ◽  
Andrea C. Premoli

We evaluated the amount and distribution of genetic variation in large and small isolated populations of Luma apiculata (DC.) Burret (Myrtaceae) in north-western Patagonia. The hypothesis tested was that isolated smaller populations were more affected by drift and isolation than large stands. Higher genetic diversity was predicted in the latter. Fresh leaf material for isozyme electrophoresis was collected from 30 individuals in four isolated and two large and continuous stands (Quetrihue Peninsula and Punta Norte, Isla Victoria). Five subpopulations were sampled in both large stands, and in addition, three regeneration gaps in Punta Norte. Eleven loci were resolved; 91% were polymorphic in at least one population. Isolated and large populations had similar levels of genetic variation. Reduced observed heterozygosity and elevated inbreeding were measured in subpopulations and regeneration gaps within dense stands. Although small populations consist of a reduced number of individuals they are mostly coastal populations nearby rivers and lakes that may maintain considerable gene flow with other faraway populations counteracting the effects of drift. In addition to potential selfing, increased inbreeding within large populations and regeneration gaps may be due to an intra-population Wahlund effect from local seedling establishment and vegetative spread, resulting in clustered cohorts of similar genotypes.


2007 ◽  
Vol 6 (8) ◽  
pp. 1421-1430 ◽  
Author(s):  
Smilja Teodorovic ◽  
John M. Braverman ◽  
Heidi G. Elmendorf

ABSTRACT Giardia lamblia, an intestinal pathogen of mammals, including humans, is a significant cause of diarrheal disease around the world. Additionally, the parasite is found on a lineage which separated early from the main branch in eukaryotic evolution. The extent of genetic diversity among G. lamblia isolates is insufficiently understood, but this knowledge is a prerequisite to better understand the role of parasite variation in disease etiology and to examine the evolution of mechanisms of genetic exchange among eukaryotes. Intraisolate genetic variation in G. lamblia has never been estimated, and previous studies on interisolate genetic variation have included a limited sample of loci. Here we report a population genetics study of intra- and interisolate genetic diversity based on six coding and four noncoding regions from nine G. lamblia isolates. Our results indicate exceedingly low levels of genetic variation in two out of three G. lamblia groups that infect humans; this variation is sufficient to allow identification of isolate-specific markers. Low genetic diversity at both coding and noncoding regions, with an overall bias towards synonymous substitutions, was discovered. Surprisingly, we found a dichotomous haplotype structure in the third, more variable G. lamblia group, represented by a haplotype shared with one of the homogenous groups and an additional group-specific haplotype. We propose that the distinct patterns of genetic-variation distribution among lineages are a consequence of the presence of genetic exchange. More broadly, our findings have implications for the regulation of gene expression, as well as the mode of reproduction in the parasite.


BMC Genetics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Marcin Słomka ◽  
Marta Sobalska-Kwapis ◽  
Małgorzata Korycka-Machała ◽  
Grzegorz Bartosz ◽  
Jarosław Dziadek ◽  
...  

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