scholarly journals 'Elaioplasts' identified as lipotubuloids in Althaea rosea, Funkia sieboldiana and Vanilla planifolia contain lipid bodies connected with microtubules

2011 ◽  
Vol 80 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Maria Kwiatkowska ◽  
Dariusz Stępiński ◽  
Katarzyna Popłońska ◽  
Agnieszka Wojtczak ◽  
Justyna Teresa Polit
Keyword(s):  
Developmental Stages ◽  
Lipid Bodies ◽  
Vanilla Planifolia ◽  
Cytoplasmic Domains ◽  
Immunogold Technique

"Elaioplasts" observed in <em>Vanilla planifolia</em>, <em>Funkia Sieboldiana</em> and <em>Althaea rosea </em>exhibit all the features characteristic of lipotubuloids earlier described in <em>Ornithogalum umbellatum</em>. They are cytoplasmic domains containing aggregates of lipid bodies connected with microtubules. The immunogold technique confirmed the presence of tubulin in this domain. These structures do not have their own membranes but they are surrounded by a tonoplast at the side of a vacuole since they invaginate into it. In cytoplasm of this domain among lipid bodies there are numerous ribosomes, ER cisternae and vesicles as well as few mitochondria, Golgi structures and microbodies while at older developmental stages there are also autolytic vacuoles. The fact that they are so similar to <em>O. umbellatum</em> lipotubuloids suggest that "elaioplasts" of <em>V. planifolia</em>, <em>F. Sieboldiana </em>and <em>A. rosea </em>can also be named lipotubuloids.

scholarly journals ON THE PLASTIDS, MITOCHONDRIA, AND OTHER CELL CONSTITUENTS DURING OÖGENESIS OF A PLANT

1966 ◽  
Vol 28 (3) ◽  
pp. 527-543 ◽  
Author(s):  
Lothar Diers
Keyword(s):  
Electron Microscopy ◽  
Cell Volume ◽  
Mother Cell ◽  
Developmental Stages ◽  
Three Dimensional ◽  
Egg Cell ◽  
Lipid Bodies ◽  
Growth Phases ◽  
Quantitative Investigation ◽  
Nucleus Volume

In the liverwort Sphaerocarpus donnellii Aust., the behavior of the cell constituents, especially of mitochondria and plastids, was studied by electron microscopy during the development of the egg and its preceding cells. A degeneration and elimination of mitochondria and plastids was not found in any of the developmental stages. In all growth phases of the archegonium, the plastids may deposit starch which becomes especially frequent in the maturing egg cell. No indications have been observed that new mitochondria or plastids generate from the nuclear evaginations, which often penetrate deeply into the cytoplasm of the maturing and fully developed eggs. A quantitative investigation based on general micrographs elucidates the numerical aspects of the cell constituents during oögenesis. With the increase of cell volume, the numbers of dictyosomes, mitochondria, plastids, and lipid bodies increase. From the stages of the mother cell of the axial row up to that of the mature egg, the cell volume enlarges about 8 times and the nucleus volume about 15 times. Simultaneously, the numbers of mitochondria and plastids increase up to 8 to 15 times. On the basis of these findings, mitochondria and plastids with three-dimensional narrow constrictions are interpreted as divisional stages.

The fine structure of conidium development in Phialocephala dimorphospora

1974 ◽  
Vol 52 (10) ◽  
pp. 2119-2128 ◽  
Author(s):  
George C. Carroll ◽  
Fanny E. Carroll
Keyword(s):  
Developmental Stages ◽  
Lateral Wall ◽  
The Body ◽  
Lipid Bodies ◽  
Dense Granules ◽  
Conidium Formation ◽  
Secondary Conidium ◽  
Apical Vesicles ◽  
Myelin Figures ◽  
Primary Conidium

Sequential developmental stages from synchronously sporulating cultures of Phialocephala dimorphospora Kendrick have been examined. Apical vesicles characterize growth of the primary conidium. These are not, however, seen during secondary conidium formation; instead, large vesicles containing minute membrane fragments develop just below the phialide neck and contribute to wall formation as they fuse with the plasmalemma. The occurrence of microtubules in the neck of the phialide is restricted to primary conidium formation. Just as the primary conidial initial begins to swell, electron-dense granules 50-75 nm in diameter arise in association with the plasmalemma. Such granules are not seen during secondary conidium formation; they are thought to be involved in pigment deposition in the collarette. During both primary and secondary conidium production the conidia go through a sequence of maturation in which the cytoplasm becomes very dense and contains lipid bodies and flocculent aggregations of electron-transparent granules. Subsequently the conidia become less electron dense and reveal a simple internal substructure consisting of a nucleus, mitochondria, micro-bodies, free ribosomes, and presumed storage bodies. The plasmalemma becomes convoluted in a network of interdigitated grooves. Septum formation occurs rapidly and involves the fusion of vesicles with the lateral wall. Mature septa are non-perforate. Although Woronin bodies have been repeatedly observed in young conidia, they are never seen in mature conidia. Myelin figures associated with mitochondria occur consistently in the body of the phialide during conidiation. These may be the source of the lipid bodies in the conidia. In phialides from cultures over 1 week old, signs of senescence are apparent. These include vacuolization of the upper portion of the phialide and the presence of many disorganized sheets of membrane. Spores still present within the collarette of such phialides are smaller than those produced early in the life of the phialide.

Actin filaments connected with the microtubules of lipotubuloids, cytoplasmic domains rich in lipid bodies and microtubules

PROTOPLASMA ◽  
2005 ◽  
Vol 226 (3-4) ◽  
pp. 163-167 ◽  
Author(s):  
M. Kwiatkowska ◽  
K. Popłońska ◽  
D. Stępiński
Keyword(s):  
Actin Filaments ◽  
Lipid Bodies ◽  
Cytoplasmic Domains

scholarly journals In silico identification, characterization and expression profile of WUSCHEL-related homeobox (WOX) gene family in Vanilla planifolia

2020 ◽  
Vol 7 (2) ◽  
pp. 206-213
Author(s):  
Thiveyarajan Victorathisayam ◽  
Madhvi Kanchan ◽  
` Himani ◽  
Thandullu R. Suriyanarayanan ◽  
Jaspreet K. Sembi ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Profile ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Alpha Helix ◽  
Vanilla Planifolia ◽  
In Planta ◽  
Specific Expression ◽  
Spatio Temporal

Vanilla planifolia is an economically important orchid, which is being commercially exploited by the food industry for the highly valued secondary metabolite vanillin. WUSCHEL-related homeobox (WOX) gene family encodes for WUSCHEL-related homeobox (WOX) transcription factors that participate in embryogenesis, organogenesis and florigenesis and in diverse plant developmental processes as well. In the present study, we analysed V. planifolia transcriptome and identified 6 WOX (VpWOX) transcripts, that encode putative WOX (VpWOX) transcription factor proteins. Domain analysis was done which indicates the presence of helix-loop-helix-turn-helix which is identifying feature of WOX gene family proteins. We executed phylogenetic clustering for the VpWOX proteins with their counterpart from the model plant Arabidopsis thaliana (AtWOX) and other closely related orchid species, Phalaenopsis equestris (PeWOX), Dendrobium catenatum (DcWOX) and Apostasia shenzhenica (AsWOX) and established their clade specific grouping. Spatio-temporal expression profile for VpWOX genes was analysed for different plant developmental stages which shows that VpWOX13 is expressing uniformly in all the developmental stages whereas, other genes have tissue specific expression. Based on gene expression patterns, we selected four VpWOX proteins and carried out secondary and tertiary structural analysis which indicates the presence of alpha helix and beta turn in the protein structure. The present study provides basic understanding of the functioning of WOX gene family in V. planifolia and paves the path for functional characterization of selected VpWOX genes in planta and in heterologous system in future for commercial utilization.

scholarly journals DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules

2012 ◽  
Vol 50 (3) ◽  
pp. 427-431 ◽  
Author(s):  
Maria Kwiatkowska ◽  
Dariusz Stępiński ◽  
Katarzyna Popłońska ◽  
Agnieszka Wojtczak ◽  
Justyna T. Polit
Keyword(s):  
Arabidopsis Thaliana ◽  
Lipid Bodies ◽  
Immunogold Technique

scholarly journals Metabolic Changes in Different Developmental Stages of Vanilla planifolia Pods

2009 ◽  
Vol 57 (17) ◽  
pp. 7651-7658 ◽  
Author(s):  
Tony Lionel Palama ◽  
Alfi Khatib ◽  
Young Hae Choi ◽  
Bertrand Payet ◽  
Isabelle Fock ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Metabolic Changes ◽  
Vanilla Planifolia

“Elaioplasts” of Haemanthus albiflos are true lipotubuloids: cytoplasmic domains rich in lipid bodies entwined by microtubules

2010 ◽  
Vol 32 (6) ◽  
pp. 1189-1196 ◽  
Author(s):  
M. Kwiatkowska ◽  
D. Stępiński ◽  
K. Popłońska ◽  
A. Wojtczak ◽  
J. Polit
Keyword(s):  
Lipid Bodies ◽  
Cytoplasmic Domains ◽  
Haemanthus Albiflos

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

Electron investigations of rhabdovirus-like particles in mexican bean beetles and crickets

1978 ◽  
Vol 36 (2) ◽  
pp. 378-379
Author(s):  
J. R. Adams ◽  
G. J Tompkins ◽  
A. M. Heimpel ◽  
E. Dougherty
Keyword(s):  
Biological Control ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Developmental Stages ◽  
Management Program ◽  
Acheta Domesticus ◽  
Epilachna Varivestis ◽  
House Cricket ◽  
Similar Morphology ◽  
Bean Beetle

As part of a continual search for potential pathogens of insects for use in biological control or on an integrated pest management program, two bacilliform virus-like particles (VLP) of similar morphology have been found in the Mexican bean beetle Epilachna varivestis Mulsant and the house cricket, Acheta domesticus (L. ).Tissues of diseased larvae and adults of E. varivestis and all developmental stages of A. domesticus were fixed according to procedures previously described. While the bean beetles displayed no external symptoms, the diseased crickets displayed a twitching and shaking of the metathoracic legs and a lowered rate of activity.Examinations of larvae and adult Mexican bean beetles collected in the field in 1976 and 1977 in Maryland and field collected specimens brought into the lab in the fall and reared through several generations revealed that specimens from each collection contained vesicles in the cytoplasm of the midgut filled with hundreds of these VLP's which were enveloped and measured approximately 16-25 nm x 55-110 nm, the shorter VLP's generally having the greater width (Fig. 1).

The Fine Structure of the Sheath of Volvox Carteri f. Weismannia

1977 ◽  
Vol 35 ◽  
pp. 346-347
Author(s):  
Regina Birchem
Keyword(s):  
Developmental Stages ◽  
Ruthenium Red ◽  
Sulfated Polysaccharides ◽  
Volvox Carteri ◽  
High Voltage Electron Microscopy ◽  
Ultrastructural Studies ◽  
Voltage Electron ◽  
Sheath Formation ◽  
And Inversion ◽  
Sheath Material

Spheroids of the green colonial alga Volvox consist of biflagellate Chlamydomonad-like cells embedded in a transparent sheath. The sheath, important as a substance through which metabolic materials, light, and the sexual inducer must pass to and from the cells, has been shown to have an ordered structure (1,2). It is composed of both protein and carbohydrate (3); studies of V. rousseletii indicate an outside layer of sulfated polysaccharides (4).Ultrastructural studies of the sheath material in developmental stages of V. carteri f. weismannia were undertaken employing variations in the standard fixation procedure, ruthenium red, diaminobenzidine, and high voltage electron microscopy. Sheath formation begins after the completion of cell division and inversion of the daughter spheroids. Golgi, rough ER, and plasma membrane are actively involved in phases of sheath synthesis (Fig. 1). Six layers of ultrastructurally differentiated sheath material have been identified.

Sign in / Sign up

Export Citation Format

Share Document