scholarly journals RAPD markers and black pine (Pinus nigra Arnold) intraspecies taxonomy - Evidence from the study of nine populations

2011 ◽  
Vol 72 (3) ◽  
pp. 249-257 ◽  
Author(s):  
Zlatko Liber ◽  
Toni Nikolić ◽  
Bożena Mitić ◽  
Zlatko Śatović
Keyword(s):  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Environmental Influences ◽  
Pinus Nigra ◽  
High Variability ◽  
Black Pine ◽  
Molecular Systematic ◽  
Anatomical Analysis

Although intraspecies researches within the black pine (<em>Pinus nigra </em>Arnold) have a long tradition, the intraspecies taxonomy, classification and chorology are still unclear. Among the numerous reasons that have caused this situation the most important are: the absence of a study that would completely cover the whole range of this species, the impossibility of connection of results of the existing detailed studies of certain areas, and the high variability of traits which have been used so far. Since the characteristics of the molecular systematic techniques could make possible the research free of the mentioned shortages, the intention of this study was to determine the relationships among nine populations of black pine using the random amplified polymorphic DNA (RAPD). The obtained results were compared to the recent results of the morphological and anatomical analysis of the leaves of the same populations. The RAPD results clearly divided the Croatian populations from populations of Austria (subsp. <em>nigra</em>) and Turkey (subsp. <em>pallasiana</em>), while among Croatian populations, as in previous study, the existence of several groups (subsp. <em>illyrica</em>, subsp. <em>dalmatica </em>and transitional population between them) was noticed. It is assumed that the optimisations conducted in this study will finally make possible estimating the relationships on the level of the whole range of the black pine and the classification based on molecular traits that are probably less dependent on environmental influences than it has been the case with the characteristics mostly used so far.

Monitoring male reproductive success in a Japanese black pine clonal seed orchard with RAPD markers

2002 ◽  
Vol 32 (6) ◽  
pp. 983-988 ◽  
Author(s):  
S Goto ◽  
F Miyahara ◽  
Y Ide
Keyword(s):  
Reproductive Success ◽  
Rapd Markers ◽  
Total Population ◽  
Random Amplified Polymorphic Dna ◽  
Seed Orchard ◽  
Male Reproductive Success ◽  
Black Pine ◽  
Clonal Seed Orchard ◽  
Japanese Black Pine ◽  
Management Techniques

Overall male reproductive success was investigated in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones, using random amplified polymorphic DNA (RAPD) markers. We genotyped all 16 of the orchard clones at selected RAPD loci by analyzing diploid needles and 10 haploid megagametophytes from each of them. In addition, the male parent of open-pollinated seedlings was identified by comparing their phenotypes with the genotypes of the orchard clones. In this way the male parents of 559 seedlings of 648 seedlings of progenies from 14 parental (orchard) clones were determined. The level of external pollen contamination in the seed orchard was estimated to be 2.2% for the total population. The effective mean selfing rate observed was 1.5%. Male reproductive success among clones varied widely, from 0.8 to 24.4%, for the total population. Our data suggest that the significant deviation in male reproductive success of individual clones from panmictic ratios may be a problem for the genetic quality in seed orchard crops. To improve the parental balance of seed orchard crops, the use of artificial management techniques such as gibberellin A4/7 treatment, supplemental mass pollination, and rearrangement of clonal ramets is recommended in seed orchards.

scholarly journals Genetic diversity of Austrian pine (Pinus nigra Arnold) populations in Serbia revealed by RAPD

2010 ◽  
Vol 62 (2) ◽  
pp. 329-336 ◽  
Author(s):  
A. Lucic ◽  
Snezana Mladenovic-Drinic ◽  
N. Stavretovic ◽  
V. Isajev ◽  
Vera Lavadinovic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pinus Nigra

The ability of random amplified polymorphic DNA (RAPD) to distinguish among populations of Austrian pine from Serbia was evaluated. Ten arbitrary 10mer primers employed in the analysis produced 113 fragments of which 100 were polymorphic (88.5%). All populations had specific RAPD phenotypes. Certain rare and genotype specific bands were identified which could be effectively used to distinguish the populations. Polymorphism in RAPD markers among P. nigra population was high and sufficient to distinguish each of the populations. The results obtained suggest that RAPD markers are valuable for the genetic diversity estimation in Pinus nigra and for the study of the divergence among populations.

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

Effect of traffic-induced air pollution on seed germination of Arizona Cypress (Cupressus arizonica Green) and Black Pine (Pinus nigra Arnold)

2020 ◽  
Vol 55 ◽  
pp. 126841
Author(s):  
Zahra Babapour Aliyar ◽  
Abbas Banj Shafiei ◽  
Nasrin Seyedi ◽  
Salar Rezapour ◽  
Saeed Musavi Moghanjugi
Keyword(s):  
Air Pollution ◽  
Seed Germination ◽  
Pinus Nigra ◽  
Black Pine ◽  
Cupressus Arizonica

scholarly journals Detection and analysis of genetic variation in Salicornieae (Chenopodiaceae) using random amplified polymorphic DNA (RAPD) markers

Taxon ◽  
1995 ◽  
Vol 44 (1) ◽  
pp. 53-63 ◽  
Author(s):  
T. Luque ◽  
C. Ruiz ◽  
J. Avalos ◽  
I. L. Calderón ◽  
M. E. Figueroa
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna

scholarly journals DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

2013 ◽  
Vol 12 (30) ◽  
pp. 4730-4735 ◽  
Author(s):  
Prasad Bylla ◽  
Gulab Khan R ◽  
Radha T ◽  
Ravi Ch ◽  
Venkataiah P ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Dna Profiling ◽  
Chilli Pepper ◽  
Capsicum Annuum L

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

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