scholarly journals Ultrastructural response of cabbage outer leaf mesophyll cells (Brassica oleracea L.) to excess of nickel

2014 ◽  
Vol 66 (3-4) ◽  
pp. 307-317 ◽  
Author(s):  
Jolanta Molas
Keyword(s):  
Brassica Oleracea ◽  
Light And Electron Microscopy ◽  
Cell Organelles ◽  
Mesophyll Cells ◽  
Volume Increase ◽  
Leaf Mesophyll ◽  
Rough Er ◽  
Cytoplasm Vacuolization ◽  
Nucleus Volume ◽  
Plasmalemma Invaginations

Changes in the structure and in the ultrastructure of cabbage outer leaf mesophyll cells [<em>Brassica oleracea</em> L.] cv. Sława from Enkhouizen were examined by means of light and electron microscopy. The examined plants were grown on the basic Murashige and Skoog medium with addition of excesive concentrations of nickel (added as NiSO<sub>4</sub> x 7H<sub>2</sub>O),i.e. Ni 5, Ni 10 and Ni 20 mg/dm<sup>3</sup>. In Ni 5 mg samples mainly adaptation changes to the conditions of stress were observed. These changes were manifested by the increase of cytoplasm content and by cytoplasm vacuolization, by the increase of nucleus and nucleous volume, nucleolus vacuolization, the increase of plasmalemma invaginations and of the amount of rough ER, by the central arrangement of smooth ER and of the thylakoids of chloroplasts; it was also shown by the growth of the number of mitochondria and of peroxisomes in the cell. In Ni 10 mg samples, apart from adaptation changes, such as the increase of the nucleus volume, increase of plasmalemma invaginations, cytoplasm and nucleolus vacuolization, degeneration changes were also observed. They concerned mainly the nucleus (the increasing amount of condensed chromatin), ER (swelling and fragmentation of rER and sER), mitochondrium (swelling and reduction of cristae), Golgi apparatus (disintegration and decay) and chloroplasts (changes of shape, swelling and reduction of thylakoids, disappearance of starch and presence of big plastoglobuli). In Ni 20 mg samples cell protoplasts were in different stages of degeneration and the cell organelles that were identifiable, were usually damaged.

STRUCTURAL-FUNCTIONAL PECULIARITIES OF LEAF MESOPHYLL CELLS OF TRITICUM AESTIVUM CULTIVARS WITH DIFFERENT COLD/HEAT TOLERANCE UNDER SHORT-TERM TEMPERATURE STRESSES

Tsitologiya ◽  
2018 ◽  
Vol 60 (2) ◽  
pp. 128-135 ◽  
Author(s):  
L. M. Babenko ◽  
◽  
N. N. Scherbatiuk ◽  
D. A. Klimchuk ◽  
I. V. Kosakovskaya ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Heat Tolerance ◽  
Short Term ◽  
Temperature Stresses ◽  
Mesophyll Cells ◽  
Leaf Mesophyll

Water transport in porous hydrogel structures analogous to leaf mesophyll cells

2014 ◽  
Vol 18 (5-6) ◽  
pp. 775-784 ◽  
Author(s):  
Sang Joon Lee ◽  
Hyejeong Kim ◽  
Sungsook Ahn
Keyword(s):  
Water Transport ◽  
Mesophyll Cells ◽  
Leaf Mesophyll ◽  
Porous Hydrogel

scholarly journals Antimicrobial Activity and Chemical Constitution of the Crude, Phenolic-Rich Extracts of Hibiscus sabdariffa, Brassica oleracea and Beta vulgaris

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4280 ◽  
Author(s):  
Seham Abdel-Shafi ◽  
Abdul-Raouf Al-Mohammadi ◽  
Mahmoud Sitohy ◽  
Basma Mosa ◽  
Ahmed Ismaiel ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Beta Vulgaris ◽  
Pathogenic Bacteria ◽  
Aspergillus Ochraceus ◽  
Total Phenolic ◽  
Klebsiella Pneumonia ◽  
Hibiscus Sabdariffa ◽  
Cell Organelles ◽  
Cell Shapes ◽  
Bacteria And Fungi

Crude, phenolic-rich extracts (CPREs) were isolated from different sources, such as Hibiscus sabdariffa (H. sabdariffa), Brassica oleracea var. capitata f. rubra (B. oleracea) and Beta vulgaris (B. vulgaris) and characterized. These CPREs showed potential antibacterial and antifungal activities. H. sabdariffa CPRE (HCPRE) is the most potent, as it inhibited all tested bacteria and fungi. Total anthocyanins content (TAC), total phenolic content (TPC) and total flavonoid content (TFC) were estimated in all three CPREs. H. sabdariffa contained 4.2 mg/100 g TAC, 2000 mg/100 g of TPC and 430 mg/100 g of TFC in a dry weight sample. GC–MS analysis of HCPRE showed 10 different active compounds that have antimicrobial effects against pathogenic bacteria and fungi, especially alcoholic compounds, triazine derivatives and esters. Scanning and transmission electron microscopy images of Staphylococcus aureus DSM 1104 and Klebsiella pneumonia ATCC 43816 treated with HCPRE (50 μg/mL) exhibited signs of asymmetric, wrinkled exterior surfaces, cell deformations and loss of cell shapes; and adherence of lysed cell content led to cell clumping, malformations, blisters, cell depressions and diminished cell numbers. This indicates death of bacterial cells and loss of cell contents. Aspergillus ochraceus EMCC516 (A. ochraceus, when treated with 100 μg/mL of HCPRE showed irregular cell organelles and cell vacuolation.

Phenolic Deposits and Kranz Syndrome in Leaf Tissues of Spotted (Euphorbia maculata) and Prostrate (Euphorbia supina) Spurge

Weed Science ◽  
1983 ◽  
Vol 31 (1) ◽  
pp. 131-136 ◽  
Author(s):  
C. Dennis Elmore ◽  
Rex N. Paul
Keyword(s):  
Electron Microscopy ◽  
Phenolic Compounds ◽  
Light And Electron Microscopy ◽  
Bundle Sheath ◽  
Fixation Technique ◽  
Mesophyll Cells ◽  
Kranz Anatomy ◽  
Bundle Sheath Cells ◽  
Leaf Tissues ◽  
Sheath Cells

Spotted spurge (Euphorbia maculataL.) and prostrate spurge (E. supinaRaf.), both in subgenusChamesyce,were examined by light and electron microscopy using a caffeine - fixation technique to sequester the phenolic pools intercellularly. Both species have typical dicotyledon-type Kranz anatomy. Sequestered phenolic pools were located in vacuoles in epidermal and mesophyll cells. Only in spotted spurge, however, were additional phenolic pools formed in bundle - sheath cells. This study was undertaken because allelopathy has been demonstrated in prostrate spurge and because phenolic compounds have been implicated in allelopathy. These results would indicate that spotted spurge should also be allelopathic.

Histopathological and ultrastructural comparison of Stemphylium sarcinaeforme and S. botryosum on red clover foliage

1978 ◽  
Vol 56 (17) ◽  
pp. 2097-2108 ◽  
Author(s):  
Verna J. Higgins ◽  
G. L. Lazarovits
Keyword(s):  
Cell Walls ◽  
Light And Electron Microscopy ◽  
Red Clover ◽  
Epidermal Cells ◽  
Blue Staining ◽  
Mesophyll Cells ◽  
Toluidine Blue Staining ◽  
Different Types ◽  
Wall Appositions ◽  
Palisade Cells

As part of a continuing study of non-host resistance, red clover leaves inoculated with the clover pathogen Stemphylium sarcinaeforme, or the closely related alfalfa pathogen S. botryosum, were examined by light and electron microscopy to compare the events occurring in the initial stages of infection. Stemphylium botryosum penetrated leaves primarily via the stomata with resultant death of the guard cells and with varying effects on adjacent epidermal cells. Appressoria were frequently formed, and although they rarely resulted in successful penetrations, the contacted epidermal cells were often markedly affected as judged by toluidine blue staining. Growth of hyphae was intercellular but very limited in its extent. At some infection sites, one to several mesophyll cells underwent extensive plasmolysis and cytoplasmic disruption. Less severely affected mesophyll cells contained large lipid bodies, abundant rough endoplasmic reticulum, and Golgi vesicles and had wall appositions at points of contact with necrotic cells or with hyphae. Stemphylium sarcinaeforme generally penetrated between or directly through the epidermal cells, causing death of the contacted cells. Hyphae grew intercellularly or intracellularly in the palisade tissue and hyphal elongation was considerably more rapid than that of S. botryosum. Palisade cells adjacent to, or containing, the hyphae underwent several different types of cytoplasmic deterioration. Mesophyll cells surrounding these areas showed the same features as comparable cells in tissue infected by S. botryosum. In tissue infected by either fungus, the exterior of host cell walls was coated with a layer of extracellular material.

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