scholarly journals Micropropagation of Urginea maritima (L.) Baker s. str.

2014 ◽  
Vol 62 (1-2) ◽  
pp. 11-15 ◽  
Author(s):  
Anna Stojakowska
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation ◽  
Urginea Maritima

A method of micropropagation of <i>Urginea maritima</i> (L.) Baker s.str. (<i>Liliaceae</i>) by adventitious shoot formation was developed. Bulb scales and leaf fragments were used as primary and secondary explants, respectively. The most favourable for shoot regeneration were media: MS supplemented with BAP or kinetin (bulb scales) and medium C containing NAA along with BAP (leaf explants). No difficulties in rooting and adapting of plants to greenhouse conditions were observed.

scholarly journals Particle Bombardment of Apple Leaf Explants Influences Adventitious Shoot Formation

HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1536-1538 ◽  
Author(s):  
P. Gercheva ◽  
R.H. Zimmerman ◽  
L.D. Owens ◽  
C. Berry ◽  
F.A. Hammerschlag
Keyword(s):  
Shoot Regeneration ◽  
Particle Bombardment ◽  
Dark Period ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Medium ◽  
Adventitious Shoot Formation ◽  
Shoot Production ◽  
And Control

Shoot regeneration from apple (Malus domestica Borkh.) leaf explants following particle bombardment at various acceleration pressures was studied. Basal leaf segments of micropropagated `Royal Gala' apple were bombarded with 1 μm gold particles, accelerated using helium pressures of 4.5, 6.2, 7.6, 9.3, or 13.8 MPa (650–2000 psi), and cultured on shoot regeneration medium consisting of N6 salts supplemented with 10 μM TDZ for 5, 10, or 20 days in darkness. Bombarded and control explants exhibited 63% to 100% shoot regeneration. With a 5-day dark period, average shoot production per explant ranged from 6.1 to 14; bombardments of 4.5 and 6.2 MPa significantly increased shoot production over the controls. With a 10-day dark period, average shoot production per explant ranged from 9.1 to 22 following bombardment at 9.3 and 6.2 MPa, respectively. Following bombardment at 6.2 MPa, 75% of the explants produced more than 20 regenerants per explant. With a 20-day dark period, average shoot production per explant ranged from 8.9 to 19 following bombardment at 13.8 MPa and following no bombardment, respectively. Shoot production per explant was significantly less than the controls following bombardments ranging from 6.2 to 13.8 MPa. Shoot production was highest per explant with particle bombardment at 6.2 MPa followed by incubation in darkness for 10 days. Chemical name used: thidiazuron (TDZ).

scholarly journals Regenerating Plants from in Vitro Culture of Black Locust Cotyledon and Leaf Explants

HortScience ◽  
1993 ◽  
Vol 28 (9) ◽  
pp. 942-945 ◽  
Author(s):  
I. Arrillaga ◽  
S.A. Merkle
Keyword(s):  
Shoot Regeneration ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Frequency ◽  
Adventitious Shoot Formation

A protocol to achieve efficient plant regeneration from juvenile black locust (Robinia pseudoacacia L.) explants is described. Direct adventitious shoots were induced from cotyledon explants on woody plant medium containing 22.2 μm BA and 0.4 μm 2,4-D. Shoots developed and new shoots were induced when the explants were transferred to medium without growth regulators. The effect of dark incubation on shoot regeneration from cotyledons indicated that 15 days of darkness resulted in a high regeneration frequency (91.7%). Adventitious shoot formation also was induced from sections of in vitro-derived leaves cultured in darkness on Murashige and Skoog medium supplemented with 4.4 μm BA and 24.6 μm IBA. A shoot regeneration frequency of 89% was obtained when explants were subcultured on a medium containing 4.4 μm BA and 0.5 μm IAA. Shoots were rooted on Schenk and Hildebrandt medium with or without IBA. Plantlets were acclimatized and grown in the greenhouse. Chemical names used: N -(phenylmethyl)-1H -purin-6-amine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA).

Adventitious shoot formation from leaf explants of Rhododendron

1992 ◽  
Vol 30 (3) ◽  
pp. 237-241 ◽  
Author(s):  
Giovanni Iapichino ◽  
Steve MeCullech ◽  
Tony H. H. Chen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

scholarly journals MICROPROPAGATION OF SELECTED DESERT-ADAPTED SPECIES

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124c-1124
Author(s):  
Dao-Shun Huang ◽  
Jingkun Ho ◽  
Ralph Backhaus
Keyword(s):  
Economic Value ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Axillary Shoot ◽  
Culture Methods ◽  
Axillary Shoot Proliferation ◽  
Adventitious Shoot Formation ◽  
Gum Tragacanth ◽  
Urginea Maritima

Tissue culture methods have been developed for a number of desert-adapted species which have potential economic value. These species include gum tragacanth (Astragalus gossypinus), candelilla (Euphorbia antisyphilitica), guayule (Parthenium argentatum), cliff rose (Cowania stansburiana and C. subintegra), bottle tree (Brachychiton populneum), red squill (Urginea maritima), Arizona agave (Agave arizonica), and spider lily (Pancratium littorale). Axillary shoot proliferation for the dicotyledenous species of this group is induced using concentrations of 2 mg/L BAP, whereas the rooting requirements vary considerably. Micropropagation of the bulb-producing species, is accomplished by aseptic culture of bulb scales in darkness, followed by shoot proliferation of the bulblets and rooting. Micropropagation of Agave requires adventitious shoot formation from a callus intermediate followed by direct caulogenesis from subculture shoots and subsequent rooting.The potential benefit of these desert-adapted species and the usefulness of the micropropagation procedures will be discussed.

Effect of genotype and medium composition on linseed (Linum usitatissimum) ovary culture

Biologia ◽  
2011 ◽  
Vol 66 (3) ◽  
Author(s):  
Natalija Burbulis ◽  
Aušra Blinstrubienė ◽  
Ramunė Kuprienė
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Linum Usitatissimum ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
Induction Medium ◽  
Ovary Culture ◽  
Adventitious Shoot Formation

AbstractBreeding linseed (Linum usitatissimum L.) using haploid techniques allows breeders to develop new cultivars in a shorter time period. Many research groups successfully created new linseed genotypes through anther culture; however ovary culture has been the subject of only a few earlier studies. In the present study, the effect of genotype and growth regulators combination on callus induction and shoots regeneration in ovary culture of nine commercially important linseed cultivars was investigated. Ovaries were cultured on modified MS medium supplemented with three different combinations of plant growth regulators. Variable callogenic responses were expressed by all of the genotypes tested on different induction media. The results suggested that specific combination of growth regulators for callus induction must be designed for each genotype. Shoot regeneration from ovary derived callus is a critical phase of the whole gynogenetic process. Differences in adventitious shoot formation frequency among genotypes were demonstrated and four responsive genotypes have been selected. Ovary derived callus from cultivar ‘Mikael’ manifested the highest adventitious shoot formation frequency with a high number of shoots per explant. The optimum ratio of growth regulators for shoot regeneration was shown to depend on the genotype. Cultivars ‘Linola’, ‘Mikael’ and ‘Szaphir’ showed the highest shoot regeneration frequency when callus had originated on induction medium supplemented with 2 mg L−1 BAP and 2 mg L−1 NAA, while combination of 1 mg L−1 BAP and 2 mg L−1 IAA promoted shoot formation in ovary-derived callus of ‘Barbara’. The highest rate of shoots per explant has been obtained in second subculture.

scholarly journals Regeneration of Pelargonium in vitro

2013 ◽  
Vol 56 (1-2) ◽  
pp. 177-190
Author(s):  
Agnieszka Wojtania ◽  
Wiesław Szafrański
Keyword(s):  
Somatic Embryos ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
High Tendency ◽  
Adventitious Shoot Formation ◽  
In Vitro Techniques ◽  
Tissue Culture Propagation

<i>Pelargonium</i> sp. has been a subject of numerous studies to deterimine the effec tiveness of in vitro techniques to produce a large number of pathogen-free plants. Regeneration of pelargonium plants from the different initial explants as well via organogenesis as via somatic embryogenesis has been obtained. The most effective adventitious shoot formation has been achieved from shoot tips and axillary buds using cytokinin or cytokinin/auxin combinations. Leaf explants, whose general have lower organogenic potency, regenerate better in the presence of thidiazuron. This growth regulator stimulate the somatic embryos production from hypocotyl and cotyledone explants too. The main problem in tissue culture propagation of <i>Pelargonium</i> has been the high tendency to formation of vigorously growing callus with low organogenic potency and rapid senescence of cultures. Moreover, the significant differen ces in requirements to the medium composition (minerals, organic compounds and growth regulators) between <i>Pelargonium</i> cultivars has been observed. This makes difficult to develop an universaI method of <i>Pelargonium</i> micropropagation.

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