Adventitious shoot formation from leaf explants of Rhododendron

1992 ◽  
Vol 30 (3) ◽  
pp. 237-241 ◽  
Author(s):  
Giovanni Iapichino ◽  
Steve MeCullech ◽  
Tony H. H. Chen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

scholarly journals Particle Bombardment of Apple Leaf Explants Influences Adventitious Shoot Formation

HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1536-1538 ◽  
Author(s):  
P. Gercheva ◽  
R.H. Zimmerman ◽  
L.D. Owens ◽  
C. Berry ◽  
F.A. Hammerschlag
Keyword(s):  
Shoot Regeneration ◽  
Particle Bombardment ◽  
Dark Period ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Medium ◽  
Adventitious Shoot Formation ◽  
Shoot Production ◽  
And Control

Shoot regeneration from apple (Malus domestica Borkh.) leaf explants following particle bombardment at various acceleration pressures was studied. Basal leaf segments of micropropagated `Royal Gala' apple were bombarded with 1 μm gold particles, accelerated using helium pressures of 4.5, 6.2, 7.6, 9.3, or 13.8 MPa (650–2000 psi), and cultured on shoot regeneration medium consisting of N6 salts supplemented with 10 μM TDZ for 5, 10, or 20 days in darkness. Bombarded and control explants exhibited 63% to 100% shoot regeneration. With a 5-day dark period, average shoot production per explant ranged from 6.1 to 14; bombardments of 4.5 and 6.2 MPa significantly increased shoot production over the controls. With a 10-day dark period, average shoot production per explant ranged from 9.1 to 22 following bombardment at 9.3 and 6.2 MPa, respectively. Following bombardment at 6.2 MPa, 75% of the explants produced more than 20 regenerants per explant. With a 20-day dark period, average shoot production per explant ranged from 8.9 to 19 following bombardment at 13.8 MPa and following no bombardment, respectively. Shoot production per explant was significantly less than the controls following bombardments ranging from 6.2 to 13.8 MPa. Shoot production was highest per explant with particle bombardment at 6.2 MPa followed by incubation in darkness for 10 days. Chemical name used: thidiazuron (TDZ).

scholarly journals Regeneration of Pelargonium in vitro

2013 ◽  
Vol 56 (1-2) ◽  
pp. 177-190
Author(s):  
Agnieszka Wojtania ◽  
Wiesław Szafrański
Keyword(s):  
Somatic Embryos ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
High Tendency ◽  
Adventitious Shoot Formation ◽  
In Vitro Techniques ◽  
Tissue Culture Propagation

<i>Pelargonium</i> sp. has been a subject of numerous studies to deterimine the effec tiveness of in vitro techniques to produce a large number of pathogen-free plants. Regeneration of pelargonium plants from the different initial explants as well via organogenesis as via somatic embryogenesis has been obtained. The most effective adventitious shoot formation has been achieved from shoot tips and axillary buds using cytokinin or cytokinin/auxin combinations. Leaf explants, whose general have lower organogenic potency, regenerate better in the presence of thidiazuron. This growth regulator stimulate the somatic embryos production from hypocotyl and cotyledone explants too. The main problem in tissue culture propagation of <i>Pelargonium</i> has been the high tendency to formation of vigorously growing callus with low organogenic potency and rapid senescence of cultures. Moreover, the significant differen ces in requirements to the medium composition (minerals, organic compounds and growth regulators) between <i>Pelargonium</i> cultivars has been observed. This makes difficult to develop an universaI method of <i>Pelargonium</i> micropropagation.

Adventitious shoot formation from in vitro leaf explants of carnation (Dianthus caryophyllus L.)

1992 ◽  
Vol 51 (3-4) ◽  
pp. 223-235 ◽  
Author(s):  
A.C. van Altvorst ◽  
H.J.J. Koehorst ◽  
T. Bruinsma ◽  
J. Jansen ◽  
J.B.M. Custers ◽  
...  
Keyword(s):  
Dianthus Caryophyllus ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

scholarly journals 861 PB 540 In Vitro Adventitious Shoot Formation and Plant Regeneration from Culantro (Eryngium foetidium L.) Leaf

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 557a-557
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Perennial Herb ◽  
Shoot Induction ◽  
Adventitious Shoot Formation ◽  
Leaf Petiole ◽  
Shoot Number ◽  
Tropical America

Culantro is a perennial herb with odor like that of corinder, native to tropical America and the West Indies. Explants were excised from leaf petiole of mature culantro plant. Explants were cultured on MS alone or supplemented with 4.4, or 13.3 uM BA with 0.5 uM NAA, or supplemented with 0.3, 1.8, 4.5, or 3 uM Thidiazuron (TDZ) wth 0.5 uM NAA. leaf explants formed callus and were transferred to the same medium for shoot induction. Only explants which were cultured on MS supplemented with 13.3 uM BA or 0.9, l.8, 4.5, or 3 UM TDZ produced shoots. Shoots were regenerated in all TDZ-containing media with high 100% frequency. Shoot number increased with the increase of TDZ concentration but shoot length decreased. Although cytokinins are reported to inhibit root initiation, regenerated shoots formed roots with 100% frequency in BA-and TDZ-containing media. Regenerated shoots were transferred to MS containing 3.9 UM TDZ for further growth. Rooted shoots were transferred to soil and normal plants were obtained.

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