scholarly journals Effect of sulphite ions on the proline and polyamine content in the bean Phaseolus vulgaris

2014 ◽  
Vol 59 (1-4) ◽  
pp. 55-64 ◽  
Author(s):  
Piotr Karolewski ◽  
Nina I. Shevyakowa
Keyword(s):  
Phaseolus Vulgaris ◽  
Free Proline ◽  
Free Polyamines ◽  
Imino Acids ◽  
Polyamine Content

The influence of sulphite (0.0125-0.2%) on changes in the levels of free proline, protein bound proline and hydroxyproline and of free polyamines in the roots and leaves of two cultivars of beans (<i>Phaseolus vulgaris</i>) differing in sensitivity to these ions was investigated. It was found that both cultivars reacted similarily, the response of the seedlings of the more sensitive cultivar occuring at lower concentrations of sulphite.The observed changes in the content of imino acids and polyamines are discussed.

Nodal distribution of free polyamines in cotton ovaries

2011 ◽  
Vol 150 (3) ◽  
pp. 365-372 ◽  
Author(s):  
A. C. BIBI ◽  
D. M. OOSTERHUIS ◽  
E. D. GONIAS ◽  
J. D. MATTICE
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Ovarian Tissue ◽  
Total Yield ◽  
Main Stem ◽  
Free Polyamines ◽  
Polyamine Content ◽  
Main Effects ◽  
Nodal Position ◽  
Nodal Distribution

SUMMARYThe nodal distribution of free polyamines (important regulators of flower induction) in cotton (Gossypium hirsutum L.) ovaries was determined by high-performance liquid chromatography (HPLC). The objective of the study was to investigate the nodal distribution of putrescine (Put), spermidine (Spd) and spermine (Sp) of cotton lines (G. hirsutum L.), and to determine whether there are differences in ovarian polyamine content among three commercial cotton cultivars. A field study was conducted in 2005 and 2006 using the cultivars FM960BR, ST5599BR and DP444BR. Free polyamines Put, Spd and Sp were determined in ovarian tissue of first-position white flowers starting at the beginning of anthesis and collected from the 7th, 9th, 11th and 13th main-stem nodes for four consecutive weeks. There was no significant nodal position by cultivar interaction; thus, the main effects were tested. Put content decreased acropetally along the main stem of the cotton plant with the highest content observed at the 7th node and the lowest at the 13th node. Spd content decreased below and above the 9th node, with the 9th node showing the highest amount of Spd and the 13th node the lowest in both years of the study. Similarly, Sp content decreased below and above the 9th node. In general, the 7th and the 9th node had the highest titre of free polyamines. Among the cultivars tested, FM960BR showed higher polyamine content in one season; however, the observation was not consistent from year to year. The highest amounts of polyamines were observed at the 7th and the 9th node of cotton and this may be associated with the known yield distribution: almost 0·80 of the total yield of cotton is derived from these nodal positions.

scholarly journals Variability in accumulation of free proline on in vitro calli of four bean (Phaseolus vulgaris L.) varieties exposed to salinity and induced moisture stress

Phyton ◽  
2006 ◽  
Vol 75 (1) ◽  
pp. 103-108 ◽  
Author(s):  
C醨denas-Avila ◽  
ML ◽  
J Verde-Star ◽  
RK Maiti ◽  
R Foroughbakhch-P ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Moisture Stress ◽  
Free Proline ◽  
Phaseolus Vulgaris L

Determination of Free Polyamines in Human Bile by High-Performance Liquid Chromatography

1993 ◽  
Vol 85 (4) ◽  
pp. 451-454 ◽  
Author(s):  
A. Hallak ◽  
R. Rosenberg ◽  
T. Gilat ◽  
G. J. Sömjen
Keyword(s):  
High Performance ◽  
The Body ◽  
Gallbladder Bile ◽  
Human Bile ◽  
T Tube ◽  
Free Polyamines ◽  
Polyamine Content ◽  
Model Bile ◽  
Bile Samples

1. Polyamines are widely distributed in the body and may have cholesterol-nucleating activity in model bile and human bile. There are only partial and scant data available on the type of polyamines in human bile. 2. In this study methods for extraction of free polyamines, benzoylation and an h.p.l.c.-based method for the quantitative determination of putrescine, cadaverine, spermidine and spermine in bile are described. The h.p.l.c. methodology was validated and compared with separation on t.l.c. after dansylation. 3. The polyamine content of 11 gallbladder bile samples and 11 T-tube bile samples was analysed, all from patients with gallstones. Polyamines were found in three out of 11 gallbladder bile samples and eight out of 11 T-tube bile samples. Putrescine levels were 0.5-287 μmol/l and cadaverine levels were 2.4-645.4 μmol/l; these were considerably higher than spermine and spermidine levels. 4. As many of these gallstones bile samples were devoid of polyamines, it is questionable whether polyamines play an important role in cholesterol nucleation in human bile.

Identification of some major cytokinins in Phaseolus vulgaris and their distribution

1996 ◽  
Vol 96 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Russell D. Hammerton ◽  
Bjorn Nicander ◽  
Elisabeth Tillberg
Keyword(s):  
Phaseolus Vulgaris

Effect of repeated severe pruning on endogenous polyamine content in hazelnut trees

1994 ◽  
Vol 92 (3) ◽  
pp. 487-492 ◽  
Author(s):  
Manuel Rey ◽  
Carmen Diaz-Sala ◽  
Roberto Rodriguez
Keyword(s):  
Polyamine Content

Chromium-induced inhibition of ethylene evolution in bean (Phaseolus vulgaris) leaves

1993 ◽  
Vol 89 (2) ◽  
pp. 404-408
Author(s):  
Charlotte Poschenrieder ◽  
Benito Gunse ◽  
Juan Barcelo
Keyword(s):  
Phaseolus Vulgaris ◽  
Ethylene Evolution

Evaluation of Azadirachtin on Arthrospira plantensis Gomont growth parameters and antioxidant enzymes

2020 ◽  
Vol 56 ◽  
pp. 8
Author(s):  
Hatice Tunca ◽  
Ali Doğru ◽  
Feray Köçkar ◽  
Burçin Önem ◽  
Tuğba Ongun Sevindik
Keyword(s):  
Enzyme Activity ◽  
Growth Parameters ◽  
Arthrospira Platensis ◽  
Proline Content ◽  
Enzyme Analysis ◽  
Free Proline ◽  
Sod Activity ◽  
Pesticide Pollution ◽  
Antioxidant Parameters ◽  
Mda Content

Azadirachtin (Aza) used as insecticide due to inhibiting growth of insects and preventing them from feeding on plants. To understand the effects of contamination of this insecticide on phototrophs, and to determine the responses of these organisms against these insecticides are extremely important in understanding how the ecosystem is affected. In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, Proline, MDA and H2O2) were determined in order to understand the effect of Aza on Arthrospira platensis Gomont. Aza was applied between 0–20 μg mL−1 concentrations for 7 days in the study. Enzyme analysis was conducted at the end of the 7th day. There was a statistically significant decrease in the absorbance of OD560 and the chlorophyll-a content in A. platensis cultures exposed to the Aza (0–20 μg mL−1) during 7 days due to the increase in pesticide levels. SOD activity decreased at 8, 16 and 20 μg mL−1 concentrations; GR enzyme activity showed a significant decrease compared to the control at a concentration of 20 μg mL−1. APX activity did not change significantly compared to control. The MDA content increased significantly at 16 and 20 μg mL−1 concentrations. The H2O2 content significantly increased at 12, 16 and 20 μg mL−1 concentrations (p < 0.05) while the free proline content decreased at 4 μg mL−1 concentration (p < 0.05). As a result, regarding the Aza concentrations used in this study may be a step to prevent pesticide pollution in the environment.

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