Determination of Free Polyamines in Human Bile by High-Performance Liquid Chromatography

1993 ◽  
Vol 85 (4) ◽  
pp. 451-454 ◽  
Author(s):  
A. Hallak ◽  
R. Rosenberg ◽  
T. Gilat ◽  
G. J. Sömjen
Keyword(s):  
High Performance ◽  
The Body ◽  
Gallbladder Bile ◽  
Human Bile ◽  
T Tube ◽  
Free Polyamines ◽  
Polyamine Content ◽  
Model Bile ◽  
Bile Samples

1. Polyamines are widely distributed in the body and may have cholesterol-nucleating activity in model bile and human bile. There are only partial and scant data available on the type of polyamines in human bile. 2. In this study methods for extraction of free polyamines, benzoylation and an h.p.l.c.-based method for the quantitative determination of putrescine, cadaverine, spermidine and spermine in bile are described. The h.p.l.c. methodology was validated and compared with separation on t.l.c. after dansylation. 3. The polyamine content of 11 gallbladder bile samples and 11 T-tube bile samples was analysed, all from patients with gallstones. Polyamines were found in three out of 11 gallbladder bile samples and eight out of 11 T-tube bile samples. Putrescine levels were 0.5-287 μmol/l and cadaverine levels were 2.4-645.4 μmol/l; these were considerably higher than spermine and spermidine levels. 4. As many of these gallstones bile samples were devoid of polyamines, it is questionable whether polyamines play an important role in cholesterol nucleation in human bile.

Human Bile Proteins

1962 ◽  
Vol 8 (3) ◽  
pp. 310-317 ◽  
Author(s):  
Arnold J Rawson
Keyword(s):  
Gamma Globulin ◽  
Serum Proteins ◽  
Gallbladder Bile ◽  
Antigenic Determinants ◽  
Human Bile ◽  
Tube Drainage ◽  
T Tube ◽  
Normal Gallbladder

Abstract A study of human bile by immunologic technics designed to identify proteins which are also found in serum shows that both albumin and gamma globulin are consistently present in both normal gallbladder bile and T-tube drainage bile. Also present in most specimens are several proteins of alpha and beta mobility sharing antigenic determinants with serum proteins.

Nodal distribution of free polyamines in cotton ovaries

2011 ◽  
Vol 150 (3) ◽  
pp. 365-372 ◽  
Author(s):  
A. C. BIBI ◽  
D. M. OOSTERHUIS ◽  
E. D. GONIAS ◽  
J. D. MATTICE
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Ovarian Tissue ◽  
Total Yield ◽  
Main Stem ◽  
Free Polyamines ◽  
Polyamine Content ◽  
Main Effects ◽  
Nodal Position ◽  
Nodal Distribution

SUMMARYThe nodal distribution of free polyamines (important regulators of flower induction) in cotton (Gossypium hirsutum L.) ovaries was determined by high-performance liquid chromatography (HPLC). The objective of the study was to investigate the nodal distribution of putrescine (Put), spermidine (Spd) and spermine (Sp) of cotton lines (G. hirsutum L.), and to determine whether there are differences in ovarian polyamine content among three commercial cotton cultivars. A field study was conducted in 2005 and 2006 using the cultivars FM960BR, ST5599BR and DP444BR. Free polyamines Put, Spd and Sp were determined in ovarian tissue of first-position white flowers starting at the beginning of anthesis and collected from the 7th, 9th, 11th and 13th main-stem nodes for four consecutive weeks. There was no significant nodal position by cultivar interaction; thus, the main effects were tested. Put content decreased acropetally along the main stem of the cotton plant with the highest content observed at the 7th node and the lowest at the 13th node. Spd content decreased below and above the 9th node, with the 9th node showing the highest amount of Spd and the 13th node the lowest in both years of the study. Similarly, Sp content decreased below and above the 9th node. In general, the 7th and the 9th node had the highest titre of free polyamines. Among the cultivars tested, FM960BR showed higher polyamine content in one season; however, the observation was not consistent from year to year. The highest amounts of polyamines were observed at the 7th and the 9th node of cotton and this may be associated with the known yield distribution: almost 0·80 of the total yield of cotton is derived from these nodal positions.

scholarly journals Penentuan β-karoten dalam buah wortel (Daucus Carota) secara kromatografi cair kinerja tinggi (U-HPLC)

2019 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Sonlimar Mangunsong ◽  
Rifqi Assiddiqy ◽  
Eka Puspa Sari ◽  
Priscila Natalia Marpaung ◽  
Rahma Arum Sari
Keyword(s):  
Free Radicals ◽  
Daucus Carota ◽  
High Performance ◽  
Calcium Salt ◽  
Beta Carotene ◽  
The Body ◽  
Organic Chemical ◽  
Chloroform Methanol ◽  
Β Carotene

The lifestyle of people who tend to be unhealthy causes a lot of free radicals in the body which can cause various diseases, especially degenerative diseases. To protect the body from attacks by free radicals, the body needs antioxidants such as β-carotene. One of the vegetables containing β-carotene is “wortel” Daucus carota, that  is very large in agriculture. The spread of beta carotene from carrots with chemical solvents has been done a lot, but without chemical solvents it has never been done. Based on the color, the content of β-carotene contained in carrots determines the content of beta carotene. The juice obtained by calcium salt was then centrifuged for 3000 rpm 15 minutes. The pellets are separated from the solution, evaporated to dryness, measured at a wavelength of 450-460. Pellets as beta-carotene calcium salts were analyzed by U-HPLC. The next pellet is measured at 460 wavelengths. The levels are determined by high performance liquid chromatography using C18 column and the mobile phase of chloroform-methanol (95:5) with a flow rate of 1 ml / minute at a wavelength of 460 nm. The content of β-carotene in the examination is up to 92,5%. The retention time obtained is 1,903 minutes. The results showed that this method can be used for the withdrawal and determination of β-carotene levels without organic chemical solvents.Pola  hidup masyarakat yang cenderung tidak sehat menyebabkan  banyak radikal bebas di dalam tubuh yang dapat mengakibatkan berbagai penyakit terutama penyakit degeneratif. Perlindungan tubuh dari serangan radikal bebas, perlu antioksidan seperti β-karoten. Salah satu sayuran  yang mengandung β-karoten adalah wortel, jumlahnya sangat banyak dalam pertanian. Berdasarkan warnanya, maka kandungan β-karoten yang terdapat dalam wortel menjadi penentu  kandungan beta karoten Metode ektraksi yang aman dari bahan pelarut kimia mutlak diperlukan.  Untuk mengetahui kandungan β-karoten dalam wortel,  lebih  dahulu dilakukan penghalusan  dengan blender, kemudian sari difilter /dipisahkan dari ampasnya. Sari  yangdiperleh ditambahkan garam kalsium kemudian disentrifus 3000 rpm  15 menit. Pelet dipisahkan dari larutannya, diuapkan hingga kering, Diukur pada panjang gelombang 450-460. Pelet sebagai garam kalsium betakaroten dianalisa dengan U-HPLC.  Pelet selanjutkan diukur pada panjang gelombang 460. ditetapkan kadarnya secara kromatografi cair kinerja tinggi menggunakan kolom C18 dan fase gerak kloroform–metanol (95:5) dengan laju alir 1 ml/menit pada panjang gelombang 460 nm. Kandungan β-karoten dalam pemeriksaan  sampai 92,5%. Waktu retensi yang diperoleh adalah 1,903 menit.  Hasil penelitian menunjukkan bahwa metode ini dapat digunakan untuk penarikan dan penetapan kadar β-karoten dalam   tanpa pelarut kimia organik.

scholarly journals ANALYSIS OF VITAMIN B-COMPLEX OF EEL FISH (ANGUILLA MARMORATA (Q.) GAIMARD) ON ELVER PHASE ORIGIN LAKE POSO

2019 ◽  
Vol 2 (2) ◽  
pp. 49
Author(s):  
Fenti Fenti ◽  
Agustinus Widodo ◽  
Jamaluddin Jamaluddin
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Vitamin B1 ◽  
Nutrient Content ◽  
The Body ◽  
Vitamin B ◽  
Anguilla Marmorata ◽  
B Vitamin ◽  
Complex Organic

Background & Objective: Vitamins are complex organic substances that are needed by the body in small amounts, usually cannot be synthesized by the body but are important in metabolic processes one of which is vitamin B. Vitamin B consists of vitamins B1, B2, B3, B6, B9 and B12. Eel is one of the fish that has a high nutrient content, one of which is vitamin B. This study aims to determine the levels of vitamin B (B1, B2, B3, B6, B9 and B12) in Eel fish (Anguilla marmorata (Q.) Gaimard) on Elver phase from Lake Poso. Material and Methods: Determination of vitamin B1, B2, B3, B6 and B9 using HPLC (High Performance Liquid Chromatography), and vitamin B12 using LC-MS (Liquid Chromatography-Mass Spectrometry) . Results: The levels of vitamin B2, B3 and B12is 0.133 mg/100g, 1.895 mg/100g, and 0.017mg/100g, whereas in vitamin B1, B6 and B9 is not detected. Conclusion: Eel fish (Anguilla marmorata (Q.) Gaimard) on Elver phase from Lake Poso can be used as a source of vitamins B2, B3 and B12.

Validation of a Method for Quantification of Lutein in Spinach Using High-Performance Liquid Chromatography: Interlaboratory Study

2020 ◽  
Vol 103 (4) ◽  
pp. 1073-1080
Author(s):  
Takefumi Sonoda ◽  
*Yusuke Hiejima ◽  
Tomohiro Koiwa ◽  
Masahiro Asano ◽  
Eiichi Kotake ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Spinacia Oleracea ◽  
The Body ◽  
Interlaboratory Study ◽  
Spinacia Oleracea L ◽  
Study Results ◽  
Relative Standard

Abstract Background Lutein is gaining attention as a strong antioxidant contained in foods. It accumulates in the human blood and retina, and is considered to play an important role in the body, especially in the eyes. Objective A method to determine the lutein content of raw spinach (Spinacia oleracea L.) was developed with the aim of its enactment as a Japanese agricultural standard (JAS) measurement method for components beneficial to human health. Methods An interlaboratory study was conducted to evaluate an analytical method for the determination of lutein in spinach. The detection limit and quantification limit of lutein for this method were 0.2 and 0.7 mg/kg, respectively. Twelve participating laboratories independently analyzed test samples (five pairs of blind duplicates) using high-performance liquid chromatography (HPLC). Results After removal of a few outliers, the repeatability relative standard deviation (RSDr), reproducibility (RSDR), and predicted RSDR of the evaluated method were 3.4–7.5, 4.6–13, and 7.5–8.5%, respectively, in a concentration range from 64.9–150 mg/kg. Conclusions The HorRat values (RSDR/predicted RSDR) of the lutein concentration were calculated to be 0.61–1.6. Highlights The study results indicate the acceptable precision of this method.

scholarly journals Simultaneous Determination of Oxyresveratrol and Resveratrol in Rat Bile and Urine by HPLC after Oral Administration of Smilax china Extract

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Hui-lian Huang ◽  
Jin-qiang Zhang ◽  
Guang-tong Chen ◽  
Zhi-qiang Lu ◽  
Na Sha ◽  
...  
Keyword(s):  
Oral Administration ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Cumulative Excretion ◽  
Rat Bile ◽  
Bile Samples

Oxyresveratrol ( trans-2,4,3′,5′-tetrahydroxystilbene, OXY) and resveratrol ( trans-3,5,4′-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 × 4.6 mm, 5 μm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively.

scholarly journals Determination of Very Low Concentration of Bisphenol A in Toys and Baby Pacifiers Using Dispersive Liquid–Liquid Microextraction by In Situ Ionic Liquid Formation and High-Performance Liquid Chromatography

2020 ◽  
Vol 13 (10) ◽  
pp. 301
Author(s):  
Yesica Vicente-Martínez ◽  
Manuel Caravaca ◽  
Antonio Soto-Meca
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
The Body ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Total Extraction

Bisphenol A (BPA) is a chemical compound used in the manufacturing of plastics and resins whose presence in the body in low concentrations can cause serious health problems. Due to this, there is a growing interest in the scientific community to develop analytical methods that allow quantifying trace concentrations of BPA in different types of samples. The determination of this compound in toys made of plastics that can be manipulated by children leads to an extra concern, because it is possible for BPA to enter the body by introducing these toys into the mouth. This work presents a novel procedure to the quickly and easily quantification of trace levels of BPA in samples of toys and pacifiers according to the current demanding regulations. The determination of very low levels of BPA was carried out by ionic liquid dispersive liquid–liquid microextraction (IL-DLLME) followed by high-performance liquid chromatography (HPLC). The formation in situ of the ionic liquid (IL) 1-octyl-3-methylimidazolium bis((trifluoromethane)sulfonyl)imide ([C8MIm] [NTf2]), was achieved by mixing 1-octyl-3-methylimidazolium chloride ([C8MIm]Cl) and lithium bis(trifluoromethanesulfonyl)imide ([NTf2]Li) aqueous solutions, reaching an instant dispersion whose cloud of microdrops allows the total extraction of BPA in the IL from aqueous solutions. After centrifugation, BPA concentration in the sedimented phase was determined by HPLC. The optimal experimental conditions for the microextraction and determination of BPA in the IL were studied. The total extraction was achieved at pH 4, heating the sample at 30 °C for 5 min, using 100 µL of IL precursor volume, and spinning after the formation of dispersion at 3000 rpm for 10 min. The enrichment factor (EF) and detection limit (LOD) reached with the procedure were 299 and 0.19 µg L−1, respectively. The relative standard deviation for ten replications at the 0.5 µg L−1 level was 5.2%. Recovery studies showed a mean value for BPA recovery percentage in the samples of 99%. Additionally, a hybrid model was applied to characterize the extraction kinetics. This simple, low cost and fast method simplifies traditional microextraction techniques, representing an outstanding alternative.

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