scholarly journals Investigations on carotenoids in lichens. XII. Some species from the Pyrenean Peninsula

2014 ◽  
Vol 55 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Bazyli Czeczuga ◽  
Blanca Cifuentes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Dry Weight ◽  
Layer Chromatography ◽  
Β Carotene

By means of column and thin-layer chromatography, the authors investigated the presence of various carotenoids in thalli of 12 species of lichens from the Pyrenean Peninsula. The following carotenoids were found: lycophyll, lycoxanthin, β-carotene, α-, β-cryptoxanthin, lutein, lutein epoxide, zeaxanthin, antheraxanthin, canthaxanthin, phoenicoxanthin, adonixanthin, α-doradexanthin, astaxanthin, diatoxanthin, neoxanthin, violaxanthin, mutatochrome, mutatoxanthin and rhodoxanthin. The total content of carotenoids ranged from 2.299 (<em>Cetraria cucullata</em>) to 39.402 mg•g<sup>-1</sup> dry weight (<em>Pseudoevernia furfuraceae</em>).

scholarly journals Investigations on carotenoids in Embryophyta. IV. The presence of apocarotenals in peatmosses

2014 ◽  
Vol 54 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Dry Weight ◽  
Layer Chromatography ◽  
Β Carotene

By means of column and thin-layer chromatography, the author investigated tit presence of various carotenoids in stems of 3 species of the Sphagnum genus. Apocarotenals (β-apo-2', β-apo-10' -carotenal and apo-12' -violaxanthal) and the following carotenoids were found: α-, β-, ;-carotene, β-cryptoxantin, lutein, lutein epoxide, zeaxanthin, adonixanthin, antheroxanthin, rhodoxanthin, rubixanthin, neoxanthin, vio-laxanthin and mutatoxanthin. The total content of carotenoids ranged from 11.954 to 41.579 mg•g<sup>-1</sup> dry weight.

Carotenoids in Lichens From Tasmanian Forests

1990 ◽  
Vol 38 (5) ◽  
pp. 517 ◽  
Author(s):  
B Czeczuga ◽  
G Kantvilas
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
First Time ◽  
Layer Chromatography ◽  
Β Carotene

Column and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 20 lichen species from Tasmanian forests: α-carotene, β-carotene, β -cryptoxanthin, lutein, zeaxanthin, 5-hydroxy-5,6-dihydrozeaxanthin, β -carotene epoxide, lutein epoxide, antheraxanthin,mutatoxanthin, violaxanthin, neoxanthin, α-doradexanthin, canthaxanthin and astaxanthin. 5-Hydroxy-5,6-dihydrozeaxanthin is reported for the first time in lichens. The total content of carotenoids ranged from 15.28�g-1 dry wt in Menegazzia confusa to 48.53�g-1 dry wt in Pseudocyphellaria glabra.

scholarly journals Carotenoids in certain lichens of Białowieża Forest

2014 ◽  
Vol 63 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
Białowieża Forest ◽  
Carotenoid Composition ◽  
Xanthoria Parietina ◽  
Layer Chromatography ◽  
Cladonia Rangiferina ◽  
Β Carotene

Column-, and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 29 lichen species from Białowieża Forest: α-carotene, β-carotene, α-cryptoxanthin, β-cryptoxanthin, lutein, 3'-epilutein, zeaxanthin, β-carotene monoepoxide, lutein epoxide, antheraxanthin, 3'-hydroxyechinenone, α-doradexanthin, canthaxanthin, astaxanthin, neoxani thin, violaxanthin and mutatoxanthin. The total content of carotenoids ranged from 16.83 (<i>Cladonia rangiferina</i>) to 92.98 µg g dry wt (<i>Xanthoria parietina</i>). There were differences in carotenoid composition, concentration of each carotenoid, and in the total content in the thalli of four species collected from niches with different insolation.

Carotenoids in Some Lichen Species from Ireland

1989 ◽  
Vol 21 (4) ◽  
pp. 363-367 ◽  
Author(s):  
B. Czeczuga ◽  
D. H. S. Richardson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Content ◽  
Lichen Species ◽  
Layer Chromatography ◽  
Β Carotene

AbstractColumn and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of seven lichen species from Ireland: α-carotene, β-carotene, β-cryptoxanthin, lutein, 3'-epilutein, adonixanthin, α-doradexanthin, canthaxanthin, astaxanthin, antheraxanthin, lutein epoxide, neoxanthin and violaxanthin. The total content of carotenoids ranged from 11.9 μg g−1 dry wt in Menegazzia terebrata to 21·3 μg g−1 dry wt in Usnea rubicunda.

scholarly journals Investigations on carotenoids in lichens. XXII. Lichens from the Upper Tracja Valley (Bulgaria)

2014 ◽  
Vol 57 (4) ◽  
pp. 447-456 ◽  
Author(s):  
Bazyli Czeczuga
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Dry Weight ◽  
Lichen Species ◽  
Total Carotenoid ◽  
Layer Chromatography ◽  
Β Carotene

Column and thin-layer chromatography were used to study the occurrence of various carotenoids in 17 lichen, species collected in the Upper Tracja Valley in Bulgaria. The following carotenoids were found: lycoxanthin, lycophyll, α-, β-carotene, rubixanthin, α-, β-cryptoxanthin, lutein, lutein epoxide, β-carotene epoxide, zeaxanthin, antheraxanthin, canthaxanthin, adonixanthin, α-doradexanthin, phoenicoxanthin, mutatochrome, mutato-xanthin, diatoxanthin and neoxanthin. The total carotenoid contents ranged from 5.737 (<em>Peltigera horizontalis</em>) to 63.659 µg g<sup>-1</sup> dry weight (<em>Xanthoria perietina</em>).

Notizen:Untersuchungen über Carotinoide in Gametangien und Sporen von Allomyces arbuscula/ Studies on Carotenoids in Gametangia and Spores of Allomyces arbuscula

1977 ◽  
Vol 32 (5-6) ◽  
pp. 442-443
Author(s):  
P. Fähnrich ◽  
L. Chenaux
Keyword(s):  
Thin Layer ◽  
Visible Light ◽  
Thin Layer Chromatography ◽  
Allomyces Arbuscula ◽  
Lag Period ◽  
Development Analysis ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids were extracted from gametophytes of Allomyces arbuscula at different periods during gametogenesis. After separation by thin-layer chromatography γ-carotene, lycopene and β-carotene were identified from their absorp­tion spectra in visible light. No other carotenoids were detected. After a lag period of 30 min following induction of gametogenesis γ-carotene was the only carotenoid present in extracts, followed by traces of β-carotene at 60 min and by lycopene at 90 min. Accumulation of carotenoids continued during the later stages of gametangia development. Analysis of pigments that had been extracted from asexual mitospores showed that these spores contained both γ-carotene and lycopene.

Resin production and glandular hairs in Beyeria viscosa (Labill.) Miq. (Euphorbiaceae)

1974 ◽  
Vol 22 (2) ◽  
pp. 195 ◽  
Author(s):  
B Dell ◽  
AJ Mccomb
Keyword(s):  
Endoplasmic Reticulum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Thick Layer ◽  
Dry Weight ◽  
Young Leaf ◽  
Mesophyll Cells ◽  
Glandular Hairs ◽  
Resin Layer ◽  
Layer Chromatography

Leaves of Beyeria viscosa secrete resin with components related to the gibberellins. Two-celled glandular hairs are well developed on the young leaf, and are coated with a thick layer of resin, which makes up almost half the dry weight associated with the young leaf. Plastids of the glandular hairs have poorly developed internal membranes but are enveloped in tubules, apparently derived from endoplasmic reticulum. As the leaf expands, resin secretion ceases; the resin layer is torn apart and is seen largely as caps over the hairs. Resin accounts for some 20% of the dry weight associated with the mature leaf. Resin components also accumulate in the epidermis and certain mesophyll cells. No significant changes take place in the chemistry of the secreted components as the leaves mature, as seen by thin-layer chromatography.

scholarly journals ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  
Keyword(s):  
Vitamin A ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Photosynthetic Bacteria ◽  
Carotenoid Pigments ◽  
Carotenoid Pigment ◽  
Bacteria And Fungi ◽  
Layer Chromatography ◽  
Β Carotene

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  

Determination of α-, β and γ-Amanitin by High Performance Thin-Layer Chromatography in Amanita phalloides (Vaill. ex Fr.) Seer, from Various Origin

1979 ◽  
Vol 34 (12) ◽  
pp. 1133-1138 ◽  
Author(s):  
Tjakko Stijve ◽  
Ruth Seeger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Dry Weight ◽  
Amanita Phalloides ◽  
Prolonged Storage ◽  
Methanolic Extracts ◽  
Layer Chromatography

A fast, sensitive high performance thin-layer chromatographic method for the determination of α-, β-, and γ-amanitin in crude, methanolic extracts of Amanita phalloides is described. The limit of detection is 50 ng of each amanitin. With this method amanitin was determined in 24 pooled samples of Amanita phalloides, collect­ed between 1970 and 1977 in Germany and Switzerland. The total amanitin content varied be­tween 2010 and 7300 mg/kg dry weight and the average value was 4430 mg/kg of which 43% was α-amanitin, 49% β-amanitin and 8% γ-amanitin. The origin of the fungi hardly influenced their amanitin content: in samples collected during the same year at different sites it fluctuated within a factor of 1.7. The amanitin content of samples from the same site, but collected in different years, maximally varied within a factor of 3.7. The partial decomposition of amanitins during prolonged storage of the lyophilized samples undoubtedly contributed to this variation. Phalloidin, which was determined by conventional thin-layer-chromatography, could not be de­tected in a sample from 1970, whereas its concentration in material collected during 1977 amount­ed to 2400 mg/kg dry weight. The toxicity of the samples (LD50 of lyophilized defatted methanolic extracts intravenously for mice) varied within a factor of 2.5.

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