scholarly journals In vitro androgenetic cultures of Hyoscyamus niger L., H. albus L. and alkaloid content assay

2014 ◽  
Vol 54 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Maria Wesołwska ◽  
Lutosław Skrzypczak
Keyword(s):  
Callus Cultures ◽  
Alkaloid Content ◽  
Quantitative Composition ◽  
Hyoscyamus Niger ◽  
Qualitative And Quantitative ◽  
Derivatives Of

<em>In vitro</em> cultures of <em>Hyoscyamus niger</em> L. and <em>H. albus</em> L. anthers were initiated which resulted in obtaining androgenectic plants and callus cultures. The leaves of these pants and the callus cultures were subjected to analysis (TLC, GC for the presence of alkaloids, derivatives of tropane. In the studied material, alkaloids of different qualitative and quantitative composition from that of ground-grown plants were found.

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry (Vaccinium macrocarpon Aiton)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1379
Author(s):  
Ieva Gudžinskaitė ◽  
Elicija Stackevičienė ◽  
Mindaugas Liaudanskas ◽  
Kristina Zymonė ◽  
Vaidotas Žvikas ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vaccinium Macrocarpon ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
American Cranberry ◽  
Fruit Samples ◽  
Phytochemical Profile ◽  
Ethanol Extracts ◽  
The Individual

The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry (Vaccinium macrocarpon Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin–Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the ‘Bain’ clone, and the highest total amount of flavonoids was found in samples of the ‘Drever’ and ‘Baiwfay’ cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the ‘Searles’ cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that ‘Woolman’, ‘Holliston’, ‘Pilgrim, and ‘Searles’ fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of ‘Baiwfay’, ‘Drever’, ‘Bain’, and ‘Bergman’ were similar in their phytochemical profile.

In vitro Propagation and Chemical and Biological Studies of the Essential Oil of Salvia przewalskii Maxim

2007 ◽  
Vol 62 (11-12) ◽  
pp. 839-848 ◽  
Author(s):  
Ewa Skała ◽  
Danuta Kalemba ◽  
Anna Wajs ◽  
Marek Róźalski ◽  
Urszula Krajewska ◽  
...  
Keyword(s):  
Essential Oil ◽  
Shoot Multiplication ◽  
Quantitative Composition ◽  
Human Leukemia ◽  
Qualitative And Quantitative ◽  
H Nmr ◽  
Regenerated Plants ◽  
Biological Studies

The procedure of Salvia przewalskii shoot multiplication and the ability of regenerated plants to produce essential oil is reported. The essential oil was obtained by hydrodistillation from leaves and flowering stems of field-grown plants, and their chemical composition was examined by GC, GC-MS and 1H NMR. The differences in yield as well as qualitative and quantitative composition between the oils isolated from in vitro and in vivo plants were observed. S. przewalskii essential oil was tested for its antimicrobial and cytotoxic properties. It was found that cytotoxicity against human leukemia HL-60 cells and antimicrobial activity (especially, against Staphylococcus aureus and S. epidermidis strains) of oils isolated from in vitro plants were higher than those for oils from in vivo S. przewalskii plants.

Chemical Composition and Biological Activity of Sapropel from the Lake Glubokoe (Tatarstan)

10.12737/5935 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Хадарцев ◽  
A. Khadartsev ◽  
Платонов ◽  
V. Platonov ◽  
Фридзон ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bactericidal Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Composition ◽  
High Biological Activity ◽  
E Coli ◽  
Lake Glubokoe ◽  
Qualitative And Quantitative ◽  
Derivatives Of ◽  
Close Genetic Relationship

Detailed information about qualitative and quantitative composition of individual components of the group sapropel from the lake Glubokoe (Tatarstan), as well as on the biological activity of different sapropel preparations were obtained by means of the methods Fourier spectroscopy, UV/Vis, NMR spectroscopy, gas chromatography-mass spectrometry, preparative Thin-layer chro-matography (TLC) with witnesses, elemental and functional analysis. Sequential scheme of extraction, acid-alkaline hydrolysis, preparative TLC source of sapropel and individual components of organic matter was developed. Amino acids, sugars, carboxylic acids, alcohols, ketones, flavonida, n-, ISO - and cycloalkanes, vitamins, derivatives of phenol, naftalan, chlorophyll, carotenoids, quinones, anthocyanins, metalloporphyrins were identified. Most of them have close genetic relationship with the source material, which is involved in sapropel formation, having a high biological activity. Biological testing of different sapropel preparations using bacteria St. Aureus, E. Coli, C. Diphythriac gravis, the fungi of the Candida albicans type were made. Significant bactericidal effect of sapropel preparations was established and it is comparable to those for synthetic antibiotics type hydro-cortisone, prednisolone.

scholarly journals Flavonoids in in vitro cultures of Astragalus hamosus

Pharmacia ◽  
2021 ◽  
Vol 68 (4) ◽  
pp. 927-931
Author(s):  
Aleksandar Shkondrov ◽  
Pavlinka Popova ◽  
Iliana Ionkova ◽  
Ilina Krasteva
Keyword(s):  
High Performance ◽  
Callus Cultures ◽  
In Vitro Cultures ◽  
Biologically Active ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Lower Quantity ◽  
Ionisation Mass Spectrometry ◽  
Initial Research

Astragalus hamosus contains valuable biologically active compounds, incl. flavonoids. The possibility for in vitro cultivation of the species as a source of important flavonoids was studied. Shoot and callus cultures were established and successfully cultivated on different nutrition media, complemented or not with growth regulators. An ultra-high performance liquid chromatography – high-resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) qualitative and quantitative analysis of non-purified methanol extracts of these cultures was performed. It was found that the cultures produced rutin in comparable quantity. Interestingly, both shoots and callus cultures accumulated the rare triglycosides alcesefoliside and mauritianin. The quantity of mauritianin, biosynthesized in shoots, was significantly higher to that in callus cultures. Alcesefoliside, was in lower quantity, compared to mauritianin. In addition, callus cultures produced alcesefoliside trice as the shoots, besides their lower level of differentiation. These findings could serve as initial research to establish the value of in vitro cultures from A. hamosus as an alternative mean of production of pharmaceutically important flavonol glycosides.

scholarly journals RAPID IN VITRO CALLOGENESIS AND PHYTOCHEMICAL SCREENING OF LEAF, STEM AND LEAF CALLUS OF MUSSAENDA FRONDOSA LINN. - A MEDICINAL PLANT.

2017 ◽  
Vol 10 (6) ◽  
pp. 81 ◽  
Author(s):  
Manasa Dj ◽  
Chandrashekar Kr ◽  
Bhagya N
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leaf Explants ◽  
Reducing Power ◽  
Callus Cultures ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative

Objective: To standardise the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem and callus cultures.Methods: The leaf explants were inoculated onto MS medium supplemented with varying concentrations of growth regulators such as 2, 4 - D, NAA, BAP, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoids and alkaloids contents of leaf, stem and callus were tested by standard methods.  The antioxidant activities were investigated using DPPH radical scavenging method and reducing power assay. The anti - inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable and fast growing callus was obtained on the medium enriched with NAA (2mg/l) + Kn (4mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10 ± 1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of Quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10g of extract). The methanolic leaf extract exhibited highest free radical scavenging activity with IC50 value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%).Conclusion: The present preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.   

scholarly journals Selection of an optimal method for screening the collection of narrow-leaved lupine held by the Vavilov Institute for the qualitative and quantitative composition of seed alkaloids

2020 ◽  
Vol 24 (8) ◽  
pp. 829-835
Author(s):  
A. V. Kushnareva ◽  
Т. V. Shelengа ◽  
I. N. Perchuk ◽  
G. P. Egorova ◽  
L. L. Malyshev ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Genetic Resources ◽  
Gene Pool ◽  
Plant Genetic Resources ◽  
Alkaloid Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Vavilov Institute ◽  
Selection Of

Narrow-leaved lupine (Lupinus аngustifolius L.) is a widely cultivated leguminous forage and green manure crop with a potential for human nutrition. However, the presence of secondary metabolites – alkaloids – in lupine seeds considerably affects the quality of raw produce, reducing its nutritive value; in addition, high concentrations of alkaloids are toxic to humans and animals. Therefore, plant breeders working with lupine need to gain knowledge about the variability of alkaloid content in seeds of different genotypes and search for the sources of their low concentrations in the crop’s gene pool. The collection of narrow-leaved lupine genetic resources held by the N.I. Vavilov Institute of Plant Genetic Resources (VIR) offers wide opportunities for such search by means of mass screening. For its part, largescale gene pool screening requires the selection of an optimal technique to measure alkaloid content in seeds, so that it would be easily reproducible and as little labor-, time- and fund-consuming as possible. The results of the search for such method are presented. Qualitative and quantitative indices were compared when target compounds had been extracted with multicomponent mixtures and individual reagents (chloroform, methanol, etc.) and the extracts analyzed using gas chromatography-mass spectrometry. High-performance liquid chromatography combined with mass spectrometry was also employed. Five major alkaloids were found to be present in all types of extracts: lupanine, 13-hydroxylupanine (dominant ones), angustifoline, sparteine, and isolupanine. The fullest extraction of alkaloids was observed when the extractant with an added alkaline agent was used (425 mg/100 g). The lowest level of extraction was registered with chloroform (216 mg/100 g). The significance of the differences was confirmed statistically.

scholarly journals Analysis of indole derivatives in methanolic extracts from mycelium of Agaricus bisporus cultured in vitro on liquid Oddoux medium

2014 ◽  
Vol 10 ◽  
pp. 66-72 ◽  
Author(s):  
Bożena Muszyńska ◽  
Katarzyna Sułkowska-Ziaja ◽  
Patrycja Hałaszczuk ◽  
Remigiusz Krężałek ◽  
Maciej Łojewski
Keyword(s):  
Agaricus Bisporus ◽  
Total Content ◽  
In Vitro Cultures ◽  
Quantitative Composition ◽  
Indole Derivatives ◽  
Fruiting Bodies ◽  
Qualitative And Quantitative ◽  
Indole Compounds ◽  
Methanolic Extracts

Methanolic extracts obtained from biomass of Agaricus bisporus (J.E. Lange) Imbach cultured in vitro were analyzed for qualitative and quantitative composition of non-hallucinogenic indole compounds in order to compare their amount with fruiting bodies of these species. Extracts demonstrated to contain six indole compounds. Contents of individual compounds ranged from 0.01 to 21.33 mg/100 g d.w. in biomass from in vitro cultures. The quantitatively dominating compounds included: 5-hydroxytryptophan (12.50 mg/100 g d.w.), L-tryptophan (14.00 mg/100 g d.w.) and serotonin (7.00 mg/100 g d.w.). Total content of the remaining indole compounds under analysis in the study was 55.32 mg/100 g d.w.

scholarly journals Seasonal variation of the qualitative and quantitative composition of triterpenic compounds and antioxidant activity in vitro in the apple extracts of cultivars grown in Lithuania

Chemija ◽  
2020 ◽  
Vol 31 (2) ◽  
Author(s):  
A. Butkevičiūtė ◽  
R. Urbštaitė ◽  
M. Liaudanskas ◽  
D. Kviklys ◽  
J. Viškelis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oleanolic Acid ◽  
Biological Effects ◽  
Biologically Active ◽  
Quantitative Composition ◽  
Phenological Stage ◽  
Phenological Stages ◽  
Qualitative And Quantitative ◽  
Triterpenic Acids

Apple (Malus domestica Borkh.) fruits are rich in phenolic glycosides, triterpenic acids and other biologically active compounds. The apples are widely used as food products due to their biologically active compounds that have specific biological effects. It is important to use high quality apples or their recycling products, so it is necessary to investigate the qualitative and quantitative composition of the bioactive compounds. The amount of triterpenic acids varies during different phenological stages of apple development and maturation. In order to determine in which phenological stage of apple growth the quantity of triterpenic acids was highest, high-performance liquid chromatography was applied. The highest total amounts of triterpenic compounds were detected at the beginning of the phenological stage of fruit development. The study showed that the highest amounts of triterpenic acids were detected at the beginning of the phenological stage of apple development, while as the apple matured, the amount of triterpenic acids decreased from 2.63 ± 0.26 mg/g to 1.6 ± 0.28 mg/g. In this study, we identified and quantified four triterpenic compounds, which by the quantitative composition of triterpenic acids could be arranged in the following ascending order: betulinic acid < corosolic acid < oleanolic acid < ursolic acid. In order to use herbal extracts for medical practice it is important to perform biological effects study in vitro and in vivo. Antioxidants with different mechanisms of action neutralize harmful reactive oxygen and nitrogen forms and enhance antioxidants protection systems. The antioxidant activity of apple extracts in vitro varied during different phenological stages of the fruit. The strongest antiradical and reductive activities were observed at the beginning of apple development. In order to determine the relationship between the antioxidant activity of the acetone extracts of apples assessed by DPPH, ABTS, CUPRAC and FRAP assays and the total amount of triterpenic acids in these extracts, a correlation analysis was carried out. The strongest positive correlation was observed between the amount of oleanolic acid and the antioxidant activity assessed by DPPH, ABTS and CUPRAC methods (respectively, r = 0.778, r = 0.784 and r = 0.720).

scholarly journals The role of poliphenols in formation of fungicidal activity and resistance to exometabolites of the fungus on the example of in vitro crops sunflower and savory

2021 ◽  
Vol 40 ◽  
pp. 01014
Author(s):  
Elena Kalashnikova ◽  
Svetlana Zaytseva ◽  
Nguen Tan Thay ◽  
Rima Kirakosyan ◽  
Mikhail Cherednichenko ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Fungicidal Activity ◽  
Sharp Decrease ◽  
Callus Cultures ◽  
Biosynthetic Activity ◽  
Qualitative And Quantitative ◽  
Ethanol Extracts

When studying the formation of secondary metabolites represented by phenolic compounds in callus cultures of sunflower plants, it was found that under in vitro conditions under the influence of a stress factor, the accumulation of polyphenols increases. It was found that the biosynthetic activity of callus cultures with respect to polyphenol increases in proportion to the concentration of the culture filtrate of the fungus (Sclerotinia Sclerotiorum) in the nutrient medium. This is confirmed by the qualitative and quantitative determination of the content of polyphenols in callus cultures. In savory plants, when introduced into culture in vitro, there is a sharp decrease in the biosynthesis of phenolic compounds. Ethanol extracts of microclones and callus cultures showed no fungicidal activity.

Sign in / Sign up

Export Citation Format

Share Document