Chemical Composition and Biological Activity of Sapropel from the Lake Glubokoe (Tatarstan)

10.12737/5935 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Хадарцев ◽  
A. Khadartsev ◽  
Платонов ◽  
V. Platonov ◽  
Фридзон ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bactericidal Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Composition ◽  
High Biological Activity ◽  
E Coli ◽  
Lake Glubokoe ◽  
Qualitative And Quantitative ◽  
Derivatives Of ◽  
Close Genetic Relationship

Detailed information about qualitative and quantitative composition of individual components of the group sapropel from the lake Glubokoe (Tatarstan), as well as on the biological activity of different sapropel preparations were obtained by means of the methods Fourier spectroscopy, UV/Vis, NMR spectroscopy, gas chromatography-mass spectrometry, preparative Thin-layer chro-matography (TLC) with witnesses, elemental and functional analysis. Sequential scheme of extraction, acid-alkaline hydrolysis, preparative TLC source of sapropel and individual components of organic matter was developed. Amino acids, sugars, carboxylic acids, alcohols, ketones, flavonida, n-, ISO - and cycloalkanes, vitamins, derivatives of phenol, naftalan, chlorophyll, carotenoids, quinones, anthocyanins, metalloporphyrins were identified. Most of them have close genetic relationship with the source material, which is involved in sapropel formation, having a high biological activity. Biological testing of different sapropel preparations using bacteria St. Aureus, E. Coli, C. Diphythriac gravis, the fungi of the Candida albicans type were made. Significant bactericidal effect of sapropel preparations was established and it is comparable to those for synthetic antibiotics type hydro-cortisone, prednisolone.

scholarly journals A New Family of Benzo[h]Chromene Based Azo Dye: Synthesis, In-Silico and DFT Studies with In Vitro Antimicrobial and Antiproliferative Assessment

2021 ◽  
Vol 22 (6) ◽  
pp. 2807
Author(s):  
Alaa S. Abd-El-Aziz ◽  
Azhaar Alsaggaf ◽  
Eman Assirey ◽  
Arshi Naqvi ◽  
Rawda M. Okasha ◽  
...  
Keyword(s):  
Biological Activity ◽  
Cell Lines ◽  
Diffusion Method ◽  
High Biological Activity ◽  
E Coli ◽  
Relationship Analysis ◽  
Chemical Descriptor ◽  
Three Component Reaction ◽  
Hct 116 ◽  
Mcf 7

The high biological activity of the chromene compounds coupled with the intriguing optical features of azo chromophores prompted our desire to construct novel derivatives of chromene incorporating azo moieties 4a-l, which have been prepared via a three-component reaction of 1-naphthalenol-4-[(4-ethoxyphenyl) azo], 1, with the benzaldehyde derivatives and malononitrile. The structural identities of the azo-chromene 4a-l were confirmed on the basis of their spectral data and elemental analysis, and a UV–visible study was performed in a Dimethylformamide (DMF) solution for these molecules. Additionally, the antimicrobial activity was investigated against four human pathogens (Gram-positive and Gram-negative bacteria) and four fungi, employing an agar well diffusion method, with their minimum inhibitory concentrations being reported. Molecules 4a, 4g, and 4h were discovered to be more efficacious against Syncephalastrum racemosum (RCMB 05922) in comparison to the reference drugs, while compounds 4b and 4h demonstrated the highest inhibitory activity against Escherichia coli (E. coli) in evaluation against the reference drugs. Moreover, their cytotoxicity was assessed against three different human cell lines, including human colon carcinoma (HCT-116), human hepatocellular carcinoma (HepG-2), and human breast adenocarcinoma (MCF-7) with a selection of molecules illustrating potency against the HCT-116 and MCF-7 cell lines. Furthermore, the molecular modeling results depicted the binding interactions of the synthesized compounds 3b and 3h in the active site of the E. coli DNA gyrase B enzyme with a clear SAR (structure–activity relationship) analysis. Lastly, the density functional theory’s (DFTs) theoretical calculations were performed to quantify the energy levels of the Frontier Molecular Orbitals (FMOs) and their energy gaps, dipole moments, and molecular electrostatic potentials. These data were utilized in the chemical descriptor estimations to confirm the biological activity.

scholarly journals The influence of meteorological factors on phytochemical composition of Artemisia pontica L.

Chemija ◽  
2020 ◽  
Vol 31 (4) ◽  
Author(s):  
Sandra Saunoriūtė ◽  
Ona Ragažinskienė ◽  
Liudas Ivanauskas ◽  
Mindaugas Marksa ◽  
Erika Šeinauskienė
Keyword(s):  
Essential Oils ◽  
Meteorological Factors ◽  
Botanical Garden ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Composition ◽  
Aromatic Plants ◽  
Medicinal And Aromatic Plants ◽  
Qualitative And Quantitative ◽  
Phytochemical Composition

In order to increase the diversity of medicinal plants and biologically active compounds accumulated in them, considerable attention is given to introduction of Artemisia L. genus plants in Lithuania. The aim of our study was to determinate the qualitative and quantitative composition of essential oils of Artemisia pontica L. introduced in Lithuania. The object of investigation – Artemisiae pontici herba were prepared in the Scientific Sector of Medicinal and Aromatic Plants, Scientific Department of Botanical Garden at Vytautas Magnus University in 2018–2019. Essential oils were separated by hydrodistillation method, their composition was analysed by gas chromatography/mass spectrometry methods in the Faculty of Pharmacy at Lithuanian University of Health Sciences in 2018–2020. The obtained results showed that a statistically significant (p > 0.05) higher amount and diversity of compounds from Artemisiae pontici herba essential oils were assessed in 2018 when HTC = 1.32. A strong correlation (r = 0.740) was assessed between the average of active temperatures and precipitation in 2019. The content of compounds was by 33.3% lower when HTC = 0.91. The other major compounds were 1,8-cineole (46.86%), 1,4-cineole (37.92%) and camphor (29.41–33.02%).

scholarly journals Selection of an optimal method for screening the collection of narrow-leaved lupine held by the Vavilov Institute for the qualitative and quantitative composition of seed alkaloids

2020 ◽  
Vol 24 (8) ◽  
pp. 829-835
Author(s):  
A. V. Kushnareva ◽  
Т. V. Shelengа ◽  
I. N. Perchuk ◽  
G. P. Egorova ◽  
L. L. Malyshev ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Genetic Resources ◽  
Gene Pool ◽  
Plant Genetic Resources ◽  
Alkaloid Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Vavilov Institute ◽  
Selection Of

Narrow-leaved lupine (Lupinus аngustifolius L.) is a widely cultivated leguminous forage and green manure crop with a potential for human nutrition. However, the presence of secondary metabolites – alkaloids – in lupine seeds considerably affects the quality of raw produce, reducing its nutritive value; in addition, high concentrations of alkaloids are toxic to humans and animals. Therefore, plant breeders working with lupine need to gain knowledge about the variability of alkaloid content in seeds of different genotypes and search for the sources of their low concentrations in the crop’s gene pool. The collection of narrow-leaved lupine genetic resources held by the N.I. Vavilov Institute of Plant Genetic Resources (VIR) offers wide opportunities for such search by means of mass screening. For its part, largescale gene pool screening requires the selection of an optimal technique to measure alkaloid content in seeds, so that it would be easily reproducible and as little labor-, time- and fund-consuming as possible. The results of the search for such method are presented. Qualitative and quantitative indices were compared when target compounds had been extracted with multicomponent mixtures and individual reagents (chloroform, methanol, etc.) and the extracts analyzed using gas chromatography-mass spectrometry. High-performance liquid chromatography combined with mass spectrometry was also employed. Five major alkaloids were found to be present in all types of extracts: lupanine, 13-hydroxylupanine (dominant ones), angustifoline, sparteine, and isolupanine. The fullest extraction of alkaloids was observed when the extractant with an added alkaline agent was used (425 mg/100 g). The lowest level of extraction was registered with chloroform (216 mg/100 g). The significance of the differences was confirmed statistically.

Gas chromatography – mass spectrometry of osulose derivatives: O-trimethylsilyl, O-acetyl, O-isopropylidene, methyl glycosides, and 1,1-dimethyl acetals

1984 ◽  
Vol 62 (5) ◽  
pp. 870-877 ◽  
Author(s):  
Pedro Antonio García Ruiz ◽  
Inmaculada Cartagena Travesedo ◽  
Antonio Soler Martinez
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Relative Abundance ◽  
Structure Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
Qualitative And Quantitative ◽  
Unambiguous Determination ◽  
Isopropylidene Derivatives ◽  
Derivatives Of

Structure determination of the O-trimethylsilyl, O-acetyl, and O-isopropylidene derivatives of some hexosuloses, pentosuloses, and one tetrosulose, as well as their methyl glycosides and 1,1-dimethyl acetals has been achieved by gas chromatography – mass spectrometry.By study based on the qualitative and quantitative differences in the 36 derivatives obtained, and their analogues prepared from tetradeuterio methanol and/or hexadeuterio acetone, and comparison with results reported in the literature, criteria for the unambiguous determination of the structural grouping are proposed. The relative abundance of each compound when the osulose forms more than one isomeric derivative in the same reaction, as well as chromatographic data for the isolated products, are reported.

scholarly journals Antibacterial Potency of Medicinal Plants including Artemisia annua and Oxalis corniculata against Multi-Drug Resistance E. coil

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Hassan Golbarg ◽  
Mohammad Javad Mehdipour Moghaddam
Keyword(s):  
Drug Resistance ◽  
Medicinal Plants ◽  
High Performance ◽  
Artemisia Annua ◽  
Inhibition Zone ◽  
Bactericidal Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Multi Drug Resistance ◽  
Food Preservatives ◽  
E Coli

Antibacterial activity of ethanolic and aqueous extracts of two medicinal plants including Oxalis corniculata (EtOc, AqOc) and Artemisia annua (EtAa, AqAa) as well as A. annua essential oil (EoAa) was investigated on multi-drug resistance (MDR) E. coli. Microdilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) as well as the inhibition zone. The phytconstituents of these products were analyzed using Reverse-phase High- performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-mass). The order of bacteriostatic and bacteriocide rate of the products can be shown as follows: EoAa>AqOc>EtAa = AqAa>EtOc, but the bactericidal effect of A. annua extracts is higher than of O. corniculata based on the MIC/MBC ratio and the order is as follows: EoAa>EtAa = AqAa>EtOc>AqOc. The most potent product, i.e. EoAa with a 56.7% inhibition of all isolates, has the potential to substitute 13 used antibiotics including oxacillin, amoxicillin, ampicillin, amoxicillin-clavulanic acid, tetracycline, streptomycin, ciprofloxacin, ceftriaxone, cefazolin, cefuroxime, cefotaxime, ceftazidime and cefixime (P <0.05). Different terpenoids were detected and measured in EoAa and catechin flavonoids in extracts of both plants, quercetin in extracts of O. corniculata but it was only possible to detect chlorogenic acid polyphenol in AqAa. Due to the antibacterial activities of the studied products, more effective than some antibiotics and their edible consumption, these products can be suggested as an alternative to some antibiotics and food preservatives to fight against MDR E. coli.

scholarly journals In vitro androgenetic cultures of Hyoscyamus niger L., H. albus L. and alkaloid content assay

2014 ◽  
Vol 54 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Maria Wesołwska ◽  
Lutosław Skrzypczak
Keyword(s):  
Callus Cultures ◽  
Alkaloid Content ◽  
Quantitative Composition ◽  
Hyoscyamus Niger ◽  
Qualitative And Quantitative ◽  
Derivatives Of

<em>In vitro</em> cultures of <em>Hyoscyamus niger</em> L. and <em>H. albus</em> L. anthers were initiated which resulted in obtaining androgenectic plants and callus cultures. The leaves of these pants and the callus cultures were subjected to analysis (TLC, GC for the presence of alkaloids, derivatives of tropane. In the studied material, alkaloids of different qualitative and quantitative composition from that of ground-grown plants were found.

scholarly journals Research of the behaviour of gidazepam and it metabolites under the conditions of acid hydrolysis

2013 ◽  
Vol 17 (3 (67) p.1) ◽  
pp. 143-147
Author(s):  
M. A. Savchenko ◽  
G. P. Petyunin
Keyword(s):  
Acid Hydrolysis ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Classical Scheme ◽  
Toxicological Research ◽  
Derivatives Of

The paper deals with results of a research of the product of gidazepam hydrolysis and the products of its metabolism, as well as their qualitative and quantitative composition, depending on the conditions of hydrolysis. The behaviour of aminobenzophenons created under the conditions of the classical scheme of a toxicological research of the derivatives of 1,4-benzodiazepine has been ascertained.

SYNTHESIS OF 3- (1,1-DIOXIDOTHIETAN-3-YL) -6-METHYLURACIL AND HYDRAZONE DERIVATIVES

2021 ◽  
pp. 181-189
Author(s):  
Melnikov A.S. ◽  
Meshcheryakova S.A.
Keyword(s):  
Biological Activity ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Statistical Processing ◽  
Biologically Active ◽  
High Biological Activity ◽  
Treatment And Prevention ◽  
Methods Of Synthesis ◽  
New Compounds ◽  
Derivatives Of

Uracil derivatives containing various N1, N3 substituents are characterized by various pharmacological and biological activities. Since the beginning of the XXI century, among them, new biologically active substances have been discovered that have pronounced antiparasitic, antibacterial, antioxidant properties. Known that modern methods of treatment and prevention of many diseases involve the inclusion of substances that have the ability to stimulate immune processes. Such compounds, in particular, include derivatives of nucleoside bases with high biological activity. Derivatives of 6-methyluracil containing a hydrazone system are widely studied in the treatment of Alzheimer's disease, antifungal, antiviral, antianginal, anti-eczema and other types of activity. The study of the alkylation of 6-methyluracil, which contains hydrazone systems in the role of pharmacophore fragments, is one of the topical trends in the synthesis of new chains of biologically active compounds. Purpose: For this purpose, we studied the alkylation of 6-methyl-3- (1,1-dioxidothietan-3-yl) pyrimidin-2,4 (1H, 3H)-dione with 1-chloropropan-2-one and the interaction of the resulting N1- 2-oxopropyl derivative with various hydrazines. Materials and Methods: The individuality of the new compounds was confirmed by the method of thin layer chromatography and determination of the melting point. The structure of the synthesized substances was confirmed by elemental analysis and NMR spectroscopy. Statistical data processing was carried out using variational analysis using the STATISTICA 8.0 software package. Student's test was chosen as a criterion for the representativeness of statistical processing. A preliminary analysis of toxicity and types of potential biological activity was carried out in silico using the resources of the Internet platform Way2Drag. Conclusions: Methods of synthesis have been developed and the most probable types of biological and pharmacological influence have been statistically processed, proceeding from the value of the probability of being active and the probability of inertness. Based on the analysis of data, modeled types of biological activity, some patterns of "structure-activity" are determined. In the conclusion, further directions of research are identified.

scholarly journals Synthesis and Characterization of New Compounds Derived from Pyrrolidine-2-One and Evaluation of their Biological Activities

2020 ◽  
Vol 23 (4) ◽  
pp. 5-12
Author(s):  
Nadia A. Betti ◽  
◽  
Redha Ib. Hussain ◽  
Sahar Ab. Kadhem ◽  
Abdul Jabar Kh. Atia ◽  
...  
Keyword(s):  
Biological Activity ◽  
Mass Spectrum ◽  
Biological Activities ◽  
The Other ◽  
Synthesis And Characterization ◽  
E Coli ◽  
Ft Ir ◽  
New Compounds ◽  
Derivatives Of

New derivatives of pyrrolidine-2-one have been prepared by lactamization of -butyrolactone GBL with hydrazine hydrate (NH2NH2(80%)) to afford (1-aminopyrrolidin-2-one) which undergo many reactions to prepare the other derivatives. The prepared derivatives were determined by utilizing their FT-IR,1H-NMR and some by Mass spectrum. These derivatives were evaluated biologically against (Staphylococcus aureusand E. coli).Some of these derivatives exhibited good biological activity against one or both kind of bacteria while some exhibited no biological activity at all.

EGF-mCherry Fusion Protein Expressed in E. coli Shows Product Heterogeneity but a High Biological Activity

Biochemistry ◽  
2019 ◽  
Vol 58 (8) ◽  
pp. 1043-1047 ◽  
Author(s):  
Rebecca C. Feiner ◽  
Ina Pennè ◽  
Benjamin Müller ◽  
Kristian M. Müller
Keyword(s):  
Biological Activity ◽  
Fusion Protein ◽  
High Biological Activity ◽  
E Coli ◽  
Product Heterogeneity
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