scholarly journals Investigations on the cytotoxic influence of zinc on Allium cepa L. roots

2014 ◽  
Vol 51 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Halina Kocik ◽  
Barbara Wojciechowska ◽  
Alicja Liguzińska
Keyword(s):  
Mitotic Activity ◽  
Allium Cepa ◽  
Zinc Chloride ◽  
Zinc Acetate ◽  
Adventitious Roots ◽  
Apical Meristem ◽  
Root Elongation ◽  
Allium Cepa L

The influence of various (0.1, 0.08, 0.04, 0.02, 0.01, 0.005, 0.0025 and 0.00125 M) zinc chloride and zinc acetate concentrations on the mitotic activity of the apical meristem of onion adventitious roots was investigated after 24 h of incubation. It was found that the tested compounds have a mitodepressive action and distinctly inhibit root elongation. The results of topochemical analysis are reported.

scholarly journals Influence of 1-p-D-arabinofuranosylcytosine on mitotic activity of apical meristem of onion (Allium cepa L.) roots

2015 ◽  
Vol 47 (1–2) ◽  
pp. 173-187 ◽  
Author(s):  
M. Kuraś ◽  
A. Malinowska
Keyword(s):  
Mitotic Activity ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Cytosine Arabinoside ◽  
Adventitious Roots ◽  
Apical Meristem ◽  
Allium Cepa L ◽  
Cells With Micronuclei

The influence of increasing cytosine arabinoside (ara-C) concentration (50, 100, 300 and 500 μg/ml) on the mitotic activity of the apical meristem of onion adventitious roots was investigated during 24-h incubation in ara-C and postincubation in water. Incubation in ara-C inhibits reversibly mitosis, the degree of inhibition being dependent on the concentration used. 50 μg/ml ara-C causes only a slight and transitional mitotic depression, while 100—500 μg/ml reduces the per cent of mitoses in various degrees after 12-h incubation whereas after 24 h all concentrations (100—500) reduce mitosis to the same level of about 2 per cent (80% inhibition). During postincubation in water there occurs after treatment with 100—500 μg/ml ara-C, a wave of highly synchronized mitoses with a peak after 12 h. The highest frequency of synchronized mitoses (60°/o) appeared in roots treated with 300 μg/ml ara-C. The main wave is preceded by a small one with a peak after 2-4 h of postincubation. This seems to be the consequence of phases S and G<sub>2</sub> inhibition by ara-C. It was found that under the influence of ara-C the per cent of mitoses with chromosome aberration and of cells with micronuclei increased only slightly.

Comparison of the Effects of He:Neon and Nd:YAG Laser Radiation on Mitotic Activity of Meristematic Cells of Allium cepa L. Bulbs

1990 ◽  
Vol 8 (6) ◽  
pp. 35-38 ◽  
Author(s):  
M. Fiol de Cuneo ◽  
S. Rubiales de Barioglio ◽  
G. Stutz ◽  
H. Juri ◽  
J. L. Lacuara
Keyword(s):  
Laser Radiation ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Nd:Yag Laser ◽  
Meristematic Cells ◽  
Allium Cepa L

scholarly journals Appraisal of Cell Cycle Delay and Cytotoxicity Inducing Potential of 3-epicaryoptin in Allium Cepa L.

2021 ◽  
Author(s):  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Cell Cycle ◽  
Medicinal Plant ◽  
Traditional Medicine ◽  
Allium Cepa ◽  
Apical Meristem ◽  
Root Apical Meristem ◽  
Cytotoxic Effects ◽  
Cell Cycle Delay ◽  
Allium Cepa L

Diterpenoid 3-epicaryoptin (C26H36O9) is abundant in leaves of Clerodendrum inerme, a traditionally used medicinal plant, having insect antifeedant activities. Here, we aim to explore its cell cycle delay and cytotoxic effects in Allium cepa root apical meristem cells. Allium cepa roots were treated with 3-epicaryoptin and colchicine for 2, 4, 4+16 h (4 h treatment followed by 16 h recovery) and the induced cell cycle delay and cytotoxic effects were analyzed. The highest metaphase cells frequency for 3-epicaryoptin and colchicine treated samples were found to be 66.2% and 82.35% respectively at 4 h treatment. Treatment of 3-epicaryoptin and colchicine increased the aberrant cells %, CA, MN, c-metaphase, and PP cells. Therefore, indiscriminate use of C. inerme in traditional medicine should be restricted and the cell cycle delay and cytotoxicity inducing effects of 3-epicaryoptin needs further exploration for its cancer chemotherapeutic applications.

scholarly journals Effect of triazophos on mitotic activity and chromosomal behavior in root meristems of Allium cepa L.

2010 ◽  
Vol 6 ◽  
pp. 4-7 ◽  
Author(s):  
Archana Pandey ◽  
Shyam R Sakya
Keyword(s):  
Mitotic Activity ◽  
Allium Cepa ◽  
Root Meristem ◽  
Plant Science ◽  
Root Tip ◽  
Allium Cepa L ◽  
Meristematic Region ◽  
Cellular Abnormalities ◽  
Tip Cells ◽  
Root Tip Cells

Effect of triazophos (an organophosphorous insecticide) on mitotic activity and chromosomal behavior in the meristematic region of root tip cells of Allium cepa L. was assessed. The insecticide showed mitotic depression and positive chromo-toxic effects. Abnormalities, such as stickiness, plasmolysed cells, equatorial plate shifting, polar shifting, irregular chromosome arrangement, precocious arms formation, bridge formation, C-metaphase, fragmentation of chromosomes, unequal cytokinesis, diagonal cytokinesis, delayed cytokinesis and formation of binucleated cells, were recorded in the chemically pretreated root meristem. Key-words: chromosomal and cellular abnormalities; cytotoxic effect; mitotic index; phase indices.DOI: 10.3126/botor.v6i0.2903 Botanica Orientalis - Journal of Plant Science (2009) 6: 4-7

scholarly journals Binding of 1-nitro-9- (3-dimethylaminopropylamino)-acridine to the DNA of the apical meristem cells of adventitious onion (Allium cepa L.) roots

2014 ◽  
Vol 53 (2) ◽  
pp. 217-222
Author(s):  
Danuta Antosiewicz
Keyword(s):  
Allium Cepa ◽  
Apical Meristem ◽  
Root Tips ◽  
Onion Root ◽  
Allium Cepa L ◽  
Covalently Bound

It was established that one half of the ledakrin (I-nitro-9-(3-dimethylaminopropylamino)-acridine) bound to the DNA in the cells of the studied onion root tips (<em>Allium cepa</em> L.), forms labile complexes with it, the remaining half is covalently attached to only one strand of the DNA. One molecule of covalently bound ledakrin falls on average to 10<sup>4</sup>-2X10<sup>4</sup> pairs of bases.

scholarly journals Effects of sodium hypochlorite on some physiological and cytogenetical parameters in Allium cepa L. exposed to salt stress

2019 ◽  
Vol 48 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Kürşat Çavuşoğlu ◽  
Fadime Doğu ◽  
Dilek Çavuşoğlu
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Sodium Hypochlorite ◽  
Seedling Growth ◽  
Chromosomal Aberrations ◽  
Root Tip ◽  
Fresh Weight ◽  
Allium Cepa L

The effects of sodium hypochlorite (NaClO) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under salt stress were studied. Salt stress considerably inhibited the seed germination and seedling growth of A. cepa. Furthermore, it markedly reduced the mitotic index in root tip meristems of the seeds and increased the number of chromosomal aberrations. Whereas, the detrimental effects of salt on the seed germination, seedling growth, mitotic activitiy and chromosomal aberrations were dramatically alleviated in varying degrees by NaClO application.

scholarly journals On the effects of secretions of saprophytic bacteria on the course of mitosis in adventitious roots of Allium cepa L.

2015 ◽  
Vol 42 (1) ◽  
pp. 171-179
Author(s):  
Z. Kobierzyńska
Keyword(s):  
Allium Cepa ◽  
Chromosome Aberrations ◽  
Adventitious Roots ◽  
Bacterial Strains ◽  
Liquid Cultures ◽  
Allium Cepa L

The influence of 21 strains of saprophytic bacteria isolated from onion cultures on the course of mitosis and on the level of chromosome aberrations in adventitious roots of <i>Allium cepa</i> L. was studied. Liquid cultures of all bacterial strains caused no changes in divisions of the cells. However, ten of the strains were responsible for disturbances in this process. The nature of these disturbances depended to a large extent on the kind of medium in which the bacteria were grown.

scholarly journals Introductory studies of bacterial effects on the course of mitosis in adventitious roots of Allium cepa L.

2015 ◽  
Vol 41 (2) ◽  
pp. 205-216
Author(s):  
J. Cebrat ◽  
K. Czerwińska ◽  
K. Bohdanowicz-Murek
Keyword(s):  
Allium Cepa ◽  
Chromosome Aberrations ◽  
Adventitious Roots ◽  
Water Culture ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Spontaneous Chromosome

The level of spontaneous chromosome aberrations and other phenomena concomitant with mitoses in the meristematic cells of <i>Allium cepa</i> L. adventitious roots grown in water, depends to a large extent on the intensity of bacteria multiplication. From the water culture two strains of bacteria, which were most numerous, were isolated - <i>Agrobacterium</i> and <i>Flavobacterium</i>. A supernatant from bacteria grown on Davis medium induced chromosome sticking together, c-mitoses and the formation of polyploid nuclei in the roots of onion.

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